LOCUS       pCAGGS-E-plcH           6968 bp    DNA     circular     11-MAR-2011
DEFINITION  LMBP 6936.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 6936
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-MAR-2011
            
COMMENT     VNTDATE|584368753|
COMMENT     VNTNAME|pCAGGS-E-plcH|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-plcH" 0 6968 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "EcoRI" "NotI" "PvuI" "SacI" "XhoI" "BglII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 4510 5851 1 6968 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 2908 0 3245 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 4689 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1073 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "BglII" "agatct" 2 0 1 2258 0 0 "")) 
COMMENT       (CObList 
COMMENT        #7=(CFSignal (CObList) "intron AG promoter" 15 0 0 5993 6917 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6303..7227") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4245 5105 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4555..5415)") 
COMMENT        #9=(CFSignal (CObList) "pMB1 ori" 33 0 1 3303 3704 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3613..4014)") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3114 3188 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3424..3498)") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3114 3188 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3424..3498") 
COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 3008 3083 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3318..3393") 
COMMENT        #13=(CFSignal (CObList) "lac promoter" 30 0 1 2784 2866 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3094..3176)") 
COMMENT        #14=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 2258 2349 0 (CStringList) (CStringList) 1 1 1 1 "2568..2659") 
COMMENT        #15=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #16=(CFSignal (CObList) "AG promoter" 29 0 0 5624 6965 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5934..7275") 
COMMENT        #17=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 5241 5618 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5551..5928") 
COMMENT        #18=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 2350 2706 0 (CStringList) (CStringList) 1 1 1 1 "2660..3016") 
COMMENT        #19=(CFSignal (CObList) "PlcH" 4 0 0 64 2256 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #20=(CFSignal (CObList) "Last modified: 11-MAR-2011" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #21=(CFSignal (CObList) "LMBP 6936" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-plcH'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/11/11 12:39" 1) 
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COMMENT                          "Last Modified: 03/11/11 12:39" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6968 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 25-SEP-2008" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                          "Misc. Marker (1 total)") 
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COMMENT                                   #72=(CLinePar 
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COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
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COMMENT                                   #77=(CLinePar 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "   complement(3094..3176)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     intron          5993..6917
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(4245..5105)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(3303..3704)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(3114..3188)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      3114..3188
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      3008..3083
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(2784..2866)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      2258..2349
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        5624..6965
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        5241..5618
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    2350..2706
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..2256
                     /vntifkey="4"
                     /label=PlcH
BASE COUNT     1417 a      2051 c      1989 g      1511 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcaatgaccg aaaactggaa attccgccgt cgaacctttc tcaagcacgg cgcccaggcc 
      121 gctaccctgg ccggcctttc cgggctgttc ccggagacgc tccggcgcgc cctggccgtc 
      181 gagccggaca tccgcaccgg aaccatccag gacgtccagc acgtggtcat cctgatgcag 
      241 gagaaccgca gcttcgatca ctacttcggc cacctcaacg gcgtccgcgg cttcaacgac 
      301 cctcgcgcgc tcaagcgcca ggacggcaag ccggtctggt accagaacta caagtacgag 
      361 ttctccccct atcactggga taccaaggtc accagcgccc agtgggtgag ttcgcagaac 
      421 catgaatggt cggccttcca cgcgatctgg aaccagggcc gcaacgacaa gtggatggcg 
      481 gtgcagtacc ccgaagccat gggctacttc aagcgcggcg acattcccta ctactacgcc 
      541 ctggccgacg ccttcaccct gtgcgaggcc taccaccagt cgatgatggg cccgaccaat 
      601 ccgaaccgcc tctaccacat gagcggacgc gccgccccca gcggcgacgg caaggacgta 
      661 cacatcggca acgacatggg cgacggcacc atcggcgcca gcggcacggt ggactggacc 
      721 acctatcccg agcggctaag cgccgctggc gtggactggc gggtctacca ggaaggcggc 
      781 taccgctcct cgtcactctg gtacctgtac gtcgacgcct actggaaata ccggctccag 
      841 gagcagaaca actacgactg caacgccctc gcctggttca ggaacttcaa gaacgctccg 
      901 cgcgattcgg acctctggca gcgcgccatg ctcgcccgcg gcgtcgacca gttgcgcaag 
      961 gatgtgcagg agaacacgct gccgcaggtg tcgtggatcg tcgcgcccta ctgctactgc 
     1021 gagcatccct ggtggggccc ctcgttcggc gagtactacg tgacccgggt gctcgaggcg 
     1081 ctgaccagca accccgaggt ctgggccagg accgtgttca tcctcaacta cgacgagggc 
     1141 gacggcttct atgaccacgc cagcgccccg gtgccgccgt ggaaggatgg cgtcggcctt 
     1201 tccacggtca gcaccgccgg cgagatcgag gtatccagcg ggctgcccat cgggctcggc 
     1261 caccgcgtcc cgctgatcgc catctcgccc tggtcgaagg gcggcaaggt cagcgccgaa 
     1321 gtgttcgacc atacctcggt gctgcgcttc ctcgaacgcc gcttcggcgt cgtcgaggaa 
     1381 aacatctcgc cctggcggcg tgccgtctgc ggcgacctga cttcgctgtt cgacttccag 
     1441 ggcgcaggcg atacccaggt cgcccccgac ctgaccaacg tcccgcagag cgacgcgcgc 
     1501 aaggaggacg cctactggca gcagttctac cggcccagtc ccaaatactg gtcctacgag 
     1561 cccaagagcc tgcccggcca ggaaaagggc cagcgcccta ccctcgcggt gccctaccag 
     1621 ttgcacgcca cgctggccct cgacatcgcc gccggcaagc tgcgcctgac cctgggcaac 
     1681 gacggcatga gcctgccggg caatccgcag ggacactccg ctgcggtatt ccaggtgcag 
     1741 ccgcgggaag tcggcaatcc gcgcttctat accgtgacca gctatccggt ggtccaggaa 
     1801 agcggagagg aactgggccg gaccctcaac gacgaactcg acgacctgct cgacgccaac 
     1861 ggccgctacg ccttcgaggt gcacggcccc aacggcttct tccgcgagtt ccacggcaac 
     1921 ctgcatctcg ccgcgcagat ggcgcggccc gaggtatcgg tcacctatca acgcaacggc 
     1981 aacctgcagt tgaacatccg caatctcggc cgcctgccgt gcagcgtgac ggtgacgccg 
     2041 aacccggcct atacccggga gggcagccgt cgctatgaac tcgaaccgaa ccaggcgatc 
     2101 agtgaagtgt ggctgttacg cagcagccag ggctggtacg acctcagcgt gaccgccagc 
     2161 aatacggaag ccaactatct ccgccgcctg gccggacacg tcgagaccgg caagccgagc 
     2221 aggagcgatc cattgctcga catcgcagcg acctgaagat ctttttccct ctgccaaaaa 
     2281 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat 
     2341 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag 
     2401 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca 
     2461 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc 
     2521 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat 
     2581 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact 
     2641 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg 
     2701 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt 
     2761 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 
     2821 cctgggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 
     2881 ccagtcggga aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc 
     2941 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 
     3001 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 
     3061 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt 
     3121 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     3181 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 
     3241 tctggatccg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 
     3301 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 
     3361 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga 
     3421 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     3481 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     3541 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     3601 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     3661 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     3721 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     3781 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     3841 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     3901 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 
     3961 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     4021 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     4081 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     4141 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     4201 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     4261 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     4321 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     4381 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     4441 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     4501 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     4561 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     4621 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     4681 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     4741 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     4801 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     4861 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     4921 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     4981 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     5041 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     5101 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     5161 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     5221 cgaaaagtgc cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac 
     5281 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 
     5341 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 
     5401 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 
     5461 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 
     5521 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 
     5581 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt 
     5641 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt 
     5701 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc 
     5761 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag 
     5821 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa 
     5881 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc 
     5941 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg 
     6001 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc 
     6061 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag 
     6121 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct 
     6181 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg 
     6241 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa 
     6301 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg 
     6361 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc 
     6421 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt 
     6481 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg 
     6541 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat 
     6601 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat 
     6661 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca 
     6721 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc 
     6781 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg 
     6841 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct 
     6901 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg 
     6961 gcaaagaa 
//
