LOCUS       pCAGGS-FLAGmA20d6        6850 bp    DNA     circular     30-SEP-2009
DEFINITION  LMBP 7158.
SOURCE      
  ORGANISM  
COMMENT     Deletion of aa 548 - 634 from mA20.
            Also introduced a completely silent Restriction Enzyme site, NheI.
COMMENT     VNTDATE|530705512|
COMMENT     VNTNAME|pCAGGS-FLAGmA20d6|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     misc_marker     13..36
                     /vntifkey="22"
                     /label=FLAG\epitope
     CDS             46..2124
                     /vntifkey="4"
                     /label=mA20
     terminator      2136..2227
                     /vntifkey="43"
                     /label=ß-globin\polyA
     misc_feature    2228..2584
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
     promoter        complement(2662..2744)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      2886..2961
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      2992..3066
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2992..3066)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(3181..3582)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(4123..4983)
                     /vntifkey="4"
                     /label=ampicillin
     promoter        5123..5500
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        5506..6847
                     /vntifkey="29"
                     /label=AG\promoter
     intron          5875..6799
                     /vntifkey="15"
                     /label=intron\AG\promoter
BASE COUNT     1573 a      1805 c      1842 g      1630 t 
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 
       61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 
      121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 
      181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 
      241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 
      301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 
      361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 
      421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 
      481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 
      541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 
      601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 
      661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 
      721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 
      781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 
      841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 
      901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 
      961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 
     1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 
     1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 
     1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 
     1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 
     1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 
     1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 
     1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 
     1441 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga 
     1501 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag 
     1561 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc 
     1621 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc tagcgcgaaa 
     1681 gctggttccc cggcccccag gttccagaac aatgtcccgt gcctgggcag ggagtgcggc 
     1741 acactcggaa gcaccatgtt tgaagggtac tgtcagaagt gtttcatcga agctcagaac 
     1801 cagagattcc atgaagcaag aagaacggaa gaacagctga gatcaagcca gcatagagac 
     1861 atgcctcgaa ctacacaggt agcctcaagg ccgaaatgtg cccgggcctc ctgcaagaac 
     1921 attctggcct gtcgcagtga ggaactctgt atggagtgcc agcacctaag ccaacgagta 
     1981 ggttctgtgg cccaccgggg tgagcccacg cctgaagagc cccctaaaca gcgctgccgg 
     2041 gcccctgctt gtgatcactt tggcaatgcc aagtgtaatg gttactgcaa tgagtgctac 
     2101 cagttcaagc agatgtatgg ctaagtgtcg acgaagatct ttttccctct gccaaaaatt 
     2161 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt 
     2221 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg 
     2281 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata 
     2341 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc 
     2401 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat 
     2461 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag 
     2521 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga 
     2581 gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga 
     2641 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 
     2701 tgggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 
     2761 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc 
     2821 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     2881 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 
     2941 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt 
     3001 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 
     3061 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 
     3121 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 
     3181 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
     3241 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcagggata acgcaggaaa 
     3301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     3361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     3421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     3481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     3541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     3601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     3661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     3721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     3781 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     3841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     3901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     3961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     4021 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     4081 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     4141 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     4201 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     4261 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     4321 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     4381 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     4441 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     4501 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     4561 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     4621 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     4681 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     4741 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     4801 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     4861 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     4921 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     4981 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     5041 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     5101 aaaagtgcca cctgggtcga tcgacattga ttattgacta gttattaata gtaatcaatt 
     5161 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 
     5221 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 
     5281 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 
     5341 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 
     5401 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 
     5461 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg 
     5521 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt 
     5581 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg 
     5641 gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag 
     5701 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 
     5761 aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc 
     5821 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc 
     5881 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt 
     5941 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg 
     6001 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg 
     6061 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg 
     6121 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac 
     6181 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc 
     6241 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt 
     6301 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag 
     6361 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg 
     6421 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 
     6481 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 
     6541 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 
     6601 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 
     6661 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 
     6721 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 
     6781 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 
     6841 tggcaaagaa 
//
