LOCUS       pCAGGS-FLAG-mA20-R546A        7099 bp    DNA     circular     16-MAR-2010
DEFINITION  LMBP 7161.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|548868033|
COMMENT     VNTNAME|pCAGGS-FLAG-mA20-R546A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "NheI" "BstXI" "HindIII" "EcoRI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (CMolPar 0 0 1 1684 1686 1 7099 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT        #1=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 1745 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 947 0 2851 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 7098 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 7002 0 0 "")) 
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COMMENT        #7=(CFSignal (CObList) "\337-globin polyA" 43 0 0 2385 2476 0 
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COMMENT        #8=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 2477 2833 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2477..2833") 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 2911 2993 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 3430 3831 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "ampicillin" 4 0 1 4372 5232 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 5372 5749 0 
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COMMENT        #18=(CFSignal (CObList) "R546A" 4 0 0 1684 1686 0 (CStringList) 
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FEATURES             Location/Qualifiers
     misc_marker     13..36
                     /vntifkey="22"
                     /label=FLAG\epitope
     CDS             46..2373
                     /vntifkey="4"
                     /label=mA20
     terminator      2385..2476
                     /vntifkey="43"
                     /label=ß-globin\polyA
     misc_feature    2477..2833
                     /vntifkey="21"
                     /label=3'\FR\of\ß-globin
     promoter        complement(2911..2993)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      3135..3210
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      3241..3315
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(3241..3315)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(3430..3831)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(4372..5232)
                     /vntifkey="4"
                     /label=ampicillin
     promoter        5372..5749
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     promoter        5755..7096
                     /vntifkey="29"
                     /label=AG\promoter
     intron          6124..7048
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             1684..1686
                     /vntifkey="4"
                     /label=R546A
                     /note="Mutated WT mA20 from CGC to GCC"
BASE COUNT     1639 a      1878 c      1896 g      1686 t 
ORIGIN
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 
       61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 
      121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 
      181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 
      241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 
      301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 
      361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 
      421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 
      481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 
      541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 
      601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 
      661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 
      721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 
      781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 
      841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 
      901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 
      961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 
     1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 
     1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 
     1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 
     1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 
     1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 
     1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 
     1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 
     1441 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga 
     1501 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag 
     1561 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc 
     1621 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc tagctgtcac 
     1681 caagcctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac 
     1741 cggactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac 
     1801 agagcaggga caagcaagtg caggaaagct ggctgcatgt attttgggac tccagaaaac 
     1861 aagggctttt gcactctatg tttcatcgaa tacagagaaa ataagcagtc tgttactgct 
     1921 agcgcgaaag ctggttcccc ggcccccagg ttccagaaca atgtcccgtg cctgggcagg 
     1981 gagtgcggca cactcggaag caccatgttt gaagggtact gtcagaagtg tttcatcgaa 
     2041 gctcagaacc agagattcca tgaagcaaga agaacggaag aacagctgag atcaagccag 
     2101 catagagaca tgcctcgaac tacacaggta gcctcaaggc cgaaatgtgc ccgggcctcc 
     2161 tgcaagaaca ttctggcctg tcgcagtgag gaactctgta tggagtgcca gcacctaagc 
     2221 caacgagtag gttctgtggc ccaccggggt gagcccacgc ctgaagagcc ccctaaacag 
     2281 cgctgccggg cccctgcttg tgatcacttt ggcaatgcca agtgtaatgg ttactgcaat 
     2341 gagtgctacc agttcaagca gatgtatggc taagtgtcga cgaagatctt tttccctctg 
     2401 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga 
     2461 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat 
     2521 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat 
     2581 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga 
     2641 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt 
     2701 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg 
     2761 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc 
     2821 tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt 
     2881 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 
     2941 tgtaaagcct gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 
     3001 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac 
     3061 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     3121 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 
     3181 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 
     3241 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     3301 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     3361 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 
     3421 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 
     3481 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcagggataa 
     3541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 
     3601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 
     3661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 
     3721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 
     3781 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 
     3841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 
     3901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 
     3961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 
     4021 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 
     4081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 
     4141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 
     4201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 
     4261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 
     4321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 
     4381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 
     4441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 
     4501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 
     4561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 
     4621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 
     4681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 
     4741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 
     4801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 
     4861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 
     4921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 
     4981 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 
     5041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 
     5101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 
     5161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 
     5221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 
     5281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
     5341 atttccccga aaagtgccac ctgggtcgat cgacattgat tattgactag ttattaatag 
     5401 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt 
     5461 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg 
     5521 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat 
     5581 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct 
     5641 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg 
     5701 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg 
     5761 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat 
     5821 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcccccc 
     5881 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca 
     5941 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg 
     6001 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg 
     6061 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca 
     6121 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga 
     6181 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 
     6241 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc 
     6301 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc 
     6361 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc 
     6421 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc 
     6481 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg 
     6541 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg 
     6601 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg 
     6661 gaaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca 
     6721 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc 
     6781 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg 
     6841 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc 
     6901 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg 
     6961 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca 
     7021 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc 
     7081 tcatcatttt ggcaaagaa 
//
