LOCUS pCAGGS-FLAG-mA20-R565A 7099 bp DNA circular 16-MAR-2010 DEFINITION LMBP 7162. SOURCE ORGANISM COMMENT VNTDATE|548868821| COMMENT VNTNAME|pCAGGS-FLAG-mA20-R565A| COMMENT VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| COMMENT VNTOAUTHORTEL|0032-9-33-13-843| COMMENT VNTOAUTHOREML|bccm.genecorner@UGent.be| COMMENT VNTOAUTHORWWW|www.genecorner.ugent.be| COMMENT VNTOAUTHORAD1|Ghent University| COMMENT VNTOAUTHORAD2|Technologiepark 927| COMMENT VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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"") COMMENT #3=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 1745 0 0 "") COMMENT #4=(CRSite (CStringList) "NheI" "gctagc" 2 0 2 1670 0 1919 0 0 "")) COMMENT (CObList COMMENT #5=(CFSignal (CObList) "FLAG epitope" 22 0 0 13 36 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "13..36") COMMENT #6=(CFSignal (CObList) "mA20" 4 0 0 46 2373 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "46..2373") COMMENT #7=(CFSignal (CObList) "\337-globin polyA" 43 0 0 2385 2476 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2385..2476") COMMENT #8=(CFSignal (CObList) "3' FR of \337-globin" 21 0 0 2477 2833 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2477..2833") COMMENT #9=(CFSignal (CObList) "lac promoter" 30 0 1 2911 2993 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2911..2993)") COMMENT #10=(CFSignal (CObList) "SV40 ori" 33 0 0 3135 3210 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "3135..3210") COMMENT #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3241 3315 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "3241..3315") COMMENT #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3241 3315 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(3241..3315)") COMMENT #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 3430 3831 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(3430..3831)") COMMENT #14=(CFSignal (CObList) "ampicillin" 4 0 1 4372 5232 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(4372..5232)") COMMENT #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 5372 5749 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "5372..5749") COMMENT #16=(CFSignal (CObList) "AG promoter" 29 0 0 5755 7096 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5755..7096") COMMENT #17=(CFSignal (CObList) "intron AG promoter" 15 0 0 6124 7048 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "6124..7048") COMMENT #18=(CFSignal (CObList) "R565A" 4 0 0 1741 1743 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) 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4.17106 0.89915 #212#) COMMENT #214=(CScratch COMMENT (CWidget 2 (1 #4# 2) 1 2 0 0 #122# 871 100) COMMENT (LOGPEN 0 6 10053171) 8 1 4.5783 1.9 0.082322 COMMENT 1) COMMENT #215=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #114# 33213332 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Nhe}I (1919)" "@N (@S)" 5 14.2394 COMMENT -3.48466 0 -10 4.09613 0.89915 #214#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #216=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -699 100) COMMENT (CObjectList) (CObjectList)) COMMENT #217=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -640 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 34406911 COMMENT (CStringList) 34341059 22077400 (CObList))) FEATURES Location/Qualifiers misc_marker 13..36 /vntifkey="22" /label=FLAG\epitope CDS 46..2373 /vntifkey="4" /label=mA20 terminator 2385..2476 /vntifkey="43" /label=ß-globin\polyA misc_feature 2477..2833 /vntifkey="21" /label=3'\FR\of\ß-globin promoter complement(2911..2993) /vntifkey="30" /label=lac\promoter rep_origin 3135..3210 /vntifkey="33" /label=SV40\ori terminator 3241..3315 /vntifkey="43" /label=SV40\polyA\early terminator complement(3241..3315) /vntifkey="43" /label=SV40\polyA\late rep_origin complement(3430..3831) /vntifkey="33" /label=pMB1\ori CDS complement(4372..5232) /vntifkey="4" /label=ampicillin promoter 5372..5749 /vntifkey="29" /label=hCMV-IE\enhancer promoter 5755..7096 /vntifkey="29" /label=AG\promoter intron 6124..7048 /vntifkey="15" /label=intron\AG\promoter CDS 1741..1743 /vntifkey="4" /label=R565A /note="WT mA20 mutated from CGG to CGC" BASE COUNT 1639 a 1879 c 1895 g 1686 t ORIGIN 1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccgccatggc tgaacaactt 61 cttcctcagg ctttgtattt gagcaatatg cggaaagctg tgaagatacg agagagaacc 121 ccagaagaca ttttcaaacc taccaatggg atcatctatc actttaaaac catgcaccga 181 tacacgctgg agatgttcag aacatgccag ttttgcccac agttccgaga gatcatccac 241 aaagcactta ttgacagaag tgtccaggct tccctggaaa gccagaagaa gctcaactgg 301 tgtcgtgaag tcaggaagct cgtggctctg aaaaccaatg gtgatggaaa ctgcctcatg 361 catgcagctt gtcagtacat gtggggtgtt caggatactg acctggtcct gaggaaggcc 421 ctctgcagca cccttaagga gacagacact cggaacttta aattccgctg gcagctggaa 481 tctctgaaat ctcaggaatt tgtggaaaca ggactttgct acgacactcg gaactggaat 541 gacgaatggg acaacttggt caaaatggca tcagcagaca cacctgcagc ccgaagtgga 601 cttcagtaca attccctgga agaaatccac atatttgtcc tcagcaacat cctcagaaga 661 cccatcattg tcatttcaga caaaatgcta agaagtttgg aatctggttc caattttgct 721 cctttgaaag tgggtgggat ttatctgcct cttcactggc ctgcccagga gtgttacaga 781 tatcccatcg tcctaggcta tgacagccag cactttgtac ccctggtgac cctgaaggac 841 agtggacctg aacttcgcgc tgttccactt gttaacagag accggggtag gtttgaagac 901 ttaaaagttc acttcttgac agatcctgag aatgagatga aggaaaagct tctaaaggag 961 tacttgatag tgatggagat ccctgtgcaa ggctgggacc acggcacgac tcacctgatc 1021 aacgctgcaa aattggatga agctaactta cccaaagaaa taaatttggt agacgattac 1081 tttgagcttg ttcagcacga atacaagaaa tggcaggaga acagcgatca ggccaggaga 1141 gcggcacatg cgcagaaccc cttggagcct tccacacccc agctatcact catggatata 1201 aaatgtgaga cacccaactg tcctttcttc atgtccgtga acactcagcc tttatgccac 1261 gaatgctcag agaggcgcca aaagaatcag agcaagctcc caaagctgaa ctcgaagcta 1321 ggccctgaag gactcccagg cgtgggactt ggctcctcaa actggagccc cgaggaaacc 1381 gctggaggac ctcattcagc cccacccaca gcacccagcc tttttctctt cagtgagacc 1441 actgcaatga agtgcaggag tcctgggtgc ccttttactt tgaatgtgca gcataatgga 1501 ttctgtgagc gttgccacgc ccggcagatt aatgccagcc acaccgcaga ccctggaaag 1561 tgccaagcct gccttcagga tgtcactcgg acctttaatg gcatctgcag tacctgtttc 1621 aaaaggacta cagcagagcc cagctccagc ctcacttcca gtatccctgc tagctgtcac 1681 caacgctcca agtctgaccc ctcacaactc atccaaagtc tcactccaca ctcttgccac 1741 cgcactggaa atgtctctcc ttctggctgc ctctcccagg ctgcacggac tccaggagac 1801 agagcaggga caagcaagtg caggaaagct ggctgcatgt attttgggac tccagaaaac 1861 aagggctttt gcactctatg tttcatcgaa tacagagaaa ataagcagtc tgttactgct 1921 agcgcgaaag ctggttcccc ggcccccagg ttccagaaca atgtcccgtg cctgggcagg 1981 gagtgcggca cactcggaag caccatgttt gaagggtact gtcagaagtg tttcatcgaa 2041 gctcagaacc agagattcca tgaagcaaga agaacggaag aacagctgag atcaagccag 2101 catagagaca tgcctcgaac tacacaggta gcctcaaggc cgaaatgtgc ccgggcctcc 2161 tgcaagaaca ttctggcctg tcgcagtgag gaactctgta tggagtgcca gcacctaagc 2221 caacgagtag gttctgtggc ccaccggggt gagcccacgc ctgaagagcc ccctaaacag 2281 cgctgccggg cccctgcttg tgatcacttt ggcaatgcca agtgtaatgg ttactgcaat 2341 gagtgctacc agttcaagca gatgtatggc taagtgtcga cgaagatctt tttccctctg 2401 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga 2461 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat 2521 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat 2581 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga 2641 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt 2701 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg 2761 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc 2821 tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt 2881 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 2941 tgtaaagcct gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 3001 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg catctcaatt agtcagcaac 3061 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 3121 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 3181 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 3241 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 3301 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 3361 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 3421 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 3481 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcagggataa 3541 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 3601 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 3661 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 3721 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 3781 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 3841 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 3901 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 3961 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 4021 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 4081 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 4141 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 4201 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 4261 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 4321 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 4381 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 4441 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 4501 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 4561 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 4621 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 4681 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 4741 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 4801 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 4861 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 4921 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 4981 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 5041 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 5101 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 5161 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 5221 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 5281 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 5341 atttccccga aaagtgccac ctgggtcgat cgacattgat tattgactag ttattaatag 5401 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt 5461 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg 5521 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat 5581 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct 5641 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg 5701 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg 5761 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat 5821 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg ggggcccccc 5881 ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggaaagg tgcggcggca 5941 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg 6001 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgttg cgcgctgcct tccccccgtg 6061 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca 6121 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga 6181 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 6241 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc 6301 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc 6361 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc 6421 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc 6481 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg 6541 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg 6601 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccgggga gggctcgggg 6661 gaaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca 6721 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc 6781 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg 6841 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc 6901 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg 6961 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca 7021 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc 7081 tcatcatttt ggcaaagaa //