LOCUS       pUC57-Seq1-mA20-R546A                   2983 bp    DNA     circular     15-DEC-2009
DEFINITION  LMBP 7165.
SOURCE      
  ORGANISM  
COMMENT     This is pUC57-Seq1-mA20-R546A vector map, provided by GenScript.
            cDNA is ligated in SmaI - EcoRV.
COMMENT     VNTDATE|537700853|
COMMENT     VNTNAME|pUC57-Seq1-mA20-R546A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 709 0 0 "") 
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COMMENT        #10=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
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COMMENT                          "Last Modified: 12/15/09 09:20" 1) 
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FEATURES             Location/Qualifiers
     CDS             432..704
                     /vntifkey="4"
                     /label=mA20
     mutation        456..458
                     /vntifkey="62"
                     /label=R546A
BASE COUNT      744 a       763 c       751 g       725 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa 
      421 tgcatctaga tagtatccct gctagctgtc accaagcctc caagtctgac ccctcacaac 
      481 tcatccaaag tctcactcca cactcttgcc accggactgg aaatgtctct ccttctggct 
      541 gcctctccca ggctgcacgg actccaggag acagagcagg gacaagcaag tgcaggaaag 
      601 ctggctgcat gtattttggg actccagaaa acaagggctt ttgcactcta tgtttcatcg 
      661 aatacagaga aaataagcag tctgttactg ctagcgcgaa agctatcgga tcccgggccc 
      721 gtcgactgca gaggcctgca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg 
      781 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 
      841 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
      901 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 
      961 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     1021 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     1081 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     1141 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     1201 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     1261 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     1321 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     1381 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     1441 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     1501 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     1561 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 
     1621 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     1681 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     1741 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     1801 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     1861 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     1921 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     1981 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     2041 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     2101 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     2161 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     2221 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     2281 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     2341 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     2401 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     2461 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     2521 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     2581 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     2641 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     2701 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     2761 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     2821 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     2881 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 
     2941 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc 
//
