LOCUS       pCAGGS-E-mCYLD-N        5833 bp    DNA     circular      8-SEP-2011
DEFINITION  LMBP 7204.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7204
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 8-SEP-2011
            
            
COMMENT     VNTDATE|600701466|
COMMENT     VNTNAME|pCAGGS-E-mCYLD-N|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-mCYLD-N" 0 5833 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "EcoRI" "NotI" "PvuI" "SacI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5833 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1773 0 2110 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 4 0 1043 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 3554 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1031 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "intron AG promoter" 15 0 0 4858 5782 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "6303..7227") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3110 3970 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(4555..5415)") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 2168 2569 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3613..4014)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1979 2053 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3424..3498)") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1979 2053 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3424..3498") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 1873 1948 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3318..3393") 
COMMENT        #12=(CFSignal (CObList) "lac promoter" 30 0 1 1649 1731 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3094..3176)") 
COMMENT        #13=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 1123 1214 0 (CStringList) (CStringList) 1 1 1 1 "2568..2659") 
COMMENT        #14=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #15=(CFSignal (CObList) "AG promoter" 29 0 0 4489 5830 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5934..7275") 
COMMENT        #16=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4106 4483 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5551..5928") 
COMMENT        #17=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 1215 1571 0 (CStringList) (CStringList) 1 1 1 1 "2660..3016") 
COMMENT        #18=(CFSignal (CObList) "mCYLD fragment" 4 0 0 64 1026 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #19=(CFSignal (CObList) "Last modified: 8-SEP-2011" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "LMBP 7204" 2 0 0 0 0 0 (CStringList) 
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COMMENT                    2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-mCYLD-N'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 09/08/11 01:31" 1) 
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COMMENT                          "Last Modified: 09/08/11 01:31" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5833 bp" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 25-SEP-2008" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
COMMENT                          1))) "Original Author") 
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COMMENT                                 "mCYLD fragment") 
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COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
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COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
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COMMENT                                 "3' flanking region of rabbit \337-globin"))) 
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COMMENT                          "Misc. Marker (1 total)") 
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COMMENT                                   #71=(CLinePar 
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COMMENT                                        "   5551..5928" 1))) "hCMV-IE enhancer") 
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COMMENT                                        "Start: 4489  End: 5830" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
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COMMENT                                   #76=(CLinePar 
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COMMENT                                        "   5934..7275" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "   complement(3094..3176)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                   #86=(CLinePar 
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COMMENT                    "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     intron          4858..5782
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(3110..3970)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2168..2569)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(1979..2053)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      1979..2053
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      1873..1948
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1649..1731)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1123..1214
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        4489..5830
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4106..4483
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1215..1571
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..1026
                     /vntifkey="4"
                     /label=mCYLD\fragment
BASE COUNT     1310 a      1457 c      1617 g      1449 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcaatgagtt caggcttatg gagccaagag aaagttactt caccctactg ggaagaacgg 
      121 attttttatc tgcttcttca agaatgcagt gtaacagaca aacaaactca gaagctgctg 
      181 aaagtaccca aagggagtat aggacagtac atccaagacc gttctgtggg gcattcaaga 
      241 gttccttcca caaaaggcaa gaaaaatcag attggattaa aaatcttgga gcaaccgcat 
      301 gcagttctgt ttgttgatga aaaggatgtt gtagaaataa atgaaaaatt cacagagtta 
      361 ctgttggcaa ttaccaactg tgaggagagg ctcagcctat ttagaaacag actccgacta 
      421 agtaaaggcc tccaggtaga cgtgggcagt cctgtgaaag tacagctgcg atctggggaa 
      481 gagaaatttc caggagttgt acgcttcaga ggacctttat tagcggagag gacggtgtcg 
      541 gggattttct ttggagtaga attattggaa gaaggtcgtg gtcaaggttt cacggatggg 
      601 gtatatcaag ggaagcagct tttccagtgt gatgaggact gtggcgtgtt tgttgcattg 
      661 gacaaactag aacttataga agatgatgac aatggattgg aaagtgattt tgcaggccca 
      721 ggagatacaa tgcaggttga acctccccct ttggaaataa actccagagt ttctttgaag 
      781 gttggagaaa gtacagaatc tggaacagta atattctgtg atgttttacc aggaaaagag 
      841 agtctaggat attttgttgg tgtggacatg gataacccta ttggcaactg ggatggaagg 
      901 tttgatggag tacagctctg tagttttgca agtgttgaaa gtacaattct cctgcacatc 
      961 aatgacatca tcccagatag cgtgacacag gaaaggaggc ctcccaaact tgcctttatg 
     1021 tcaagagacc tcgaggtcga ggaattcact cctcaggtgc aggctgccta tcagaaggtg 
     1081 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc 
     1141 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa 
     1201 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat 
     1261 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat 
     1321 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa 
     1381 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt 
     1441 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt 
     1501 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc 
     1561 ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc 
     1621 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     1681 taaagcctgg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 
     1741 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca 
     1801 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 
     1861 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 
     1921 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa 
     1981 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     2041 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 
     2101 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     2161 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     2221 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc agggataacg 
     2281 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 
     2341 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 
     2401 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 
     2461 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 
     2521 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 
     2581 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 
     2641 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 
     2701 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 
     2761 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga 
     2821 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 
     2881 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 
     2941 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 
     3001 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 
     3061 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 
     3121 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 
     3181 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 
     3241 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 
     3301 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 
     3361 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 
     3421 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 
     3481 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 
     3541 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 
     3601 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 
     3661 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 
     3721 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
     3781 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
     3841 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
     3901 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
     3961 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 
     4021 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 
     4081 ttccccgaaa agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca 
     4141 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta 
     4201 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat 
     4261 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 
     4321 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac 
     4381 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt 
     4441 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc 
     4501 acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt 
     4561 attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcc ccccccaggc 
     4621 ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat 
     4681 cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat 
     4741 aaaaagcgaa gcgcgcggcg ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc 
     4801 tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg 
     4861 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt 
     4921 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg 
     4981 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc 
     5041 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt 
     5101 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg 
     5161 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg 
     5221 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg 
     5281 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg 
     5341 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggaaggg 
     5401 gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct 
     5461 tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc 
     5521 gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc 
     5581 cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct 
     5641 ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag 
     5701 ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca 
     5761 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca 
     5821 ttttggcaaa gaa 
//
