LOCUS       SHC005_(sh-eGFP)        7086 bp    DNA     circular      6-DEC-2016
DEFINITION  LMBP 7275.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7275
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 06-DEC-2016
            
COMMENT     VNTDATE|590583528|
COMMENT     VNTNAME|SHC005 (sh-eGFP)|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTREPLTYPE|Virus
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "SHC005 (sh-eGFP)" 0 7086 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "AgeI" "EcoRI" "HindIII" "PvuII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 1 0 0 0 0 1 7086 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 6823 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 6881 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 5 1391 0 4735 0 5047 0 
COMMENT            5604 0 6188 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 1221 0 1997 0 4510 0 0 
COMMENT            "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "puromycin resistance" 4 0 0 533 1132 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "529..1128") 
COMMENT        #5=(CFSignal (CObList) "SV40 ori" 33 0 0 1775 1850 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1771..1846") 
COMMENT        #6=(CFSignal (CObList) "T7 promoter" 30 0 1 1884 1900 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1880..1896)") 
COMMENT        #7=(CFSignal (CObList) "f1 ori" 33 0 0 2068 2523 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2064..2519") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 0 2654 3514 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2650..3510") 
COMMENT        #9=(CFSignal (CObList) "pMB1 ori" 33 0 0 4055 4457 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4051..4453") 
COMMENT        #10=(CFSignal (CObList) "lac promoter" 30 0 0 4549 4632 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4545..4628") 
COMMENT        #11=(CFSignal (CObList) "T3 promoter" 30 0 0 4696 4712 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4692..4708") 
COMMENT        #12=(CFSignal (CObList) "RSV/HIV-1 5' LTR" 29 0 0 4821 5149 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4817..5145") 
COMMENT        #13=(CFSignal (CObList) "HIV-1 psi packaging signal" 21 0 0 5260 5304 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5256..5300") 
COMMENT        #14=(CFSignal (CObList) "HIV-1 Rev response element" 21 0 0 5815 6048 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5811..6044") 
COMMENT        #15=(CFSignal (CObList) "RSV/HIV-1 dU3/3' LTR" 43 0 0 1259 1493 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1255..1489") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1565 1639 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1561..1635") 
COMMENT        #17=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1565 1639 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1561..1635)") 
COMMENT        #18=(CFSignal (CObList) "hU6 promoter" 29 0 0 6576 6822 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6572..6818") 
COMMENT        #19=(CFSignal (CObList) "HIV-1 cPPT" 21 0 0 6925 6940 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6921..6936") 
COMMENT        #20=(CFSignal (CObList) "HIV-1 cPPT" 21 0 0 1243 1258 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1239..1254") 
COMMENT        #21=(CFSignal (CObList) "hPGK1 promoter" 29 0 0 1 352 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1..507") 
COMMENT        #22=(CFSignal (CObList) "sh-EGFP" 21 0 0 6827 6874 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6819..6875")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #24=(CFSignal (CObList) "Last modified: 06-DEC-2016" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid Phage Virus 'SHC005 (sh-eGFP)'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 05/19/11 10:58" 1) 
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COMMENT                          "Last Modified: 12/06/16 12:07" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7086 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 21-SEP-2016" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 7275") 
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COMMENT                                   #73=(CLinePar 
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COMMENT                                   #77=(CLinePar 
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COMMENT                          "Misc. Feature (5 total)") 
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COMMENT                                   #82=(CLinePar 
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COMMENT                                        "RSV promoter: 4817-4964 / HIV-1 5' LTR: 4965-5145" 
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COMMENT                          "Promoter Eukaryotic (3 total)") 
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COMMENT                                           "   complement(1561..1635)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                                            " N1: 6823 " 1))))) "AgeI: 1 site") 
COMMENT                            1 5 "ACCGGT" "TGGCCA") 
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FEATURES             Location/Qualifiers
     CDS             533..1132
                     /vntifkey="4"
                     /label=puromycin\resistance
     rep_origin      1775..1850
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1884..1900)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      2068..2523
                     /vntifkey="33"
                     /label=f1\ori
     CDS             2654..3514
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      4055..4457
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        4549..4632
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        4696..4712
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        4821..5149
                     /vntifkey="29"
                     /label=RSV/HIV-1\5'\LTR
                     /note="RSV promoter: 4817-4964 / HIV-1 5' LTR: 4965-5145"
     misc_feature    5260..5304
                     /vntifkey="21"
                     /label=HIV-1\psi\packaging\signal
     misc_feature    5815..6048
                     /vntifkey="21"
                     /label=HIV-1\Rev\response\element
     terminator      1259..1493
                     /vntifkey="43"
                     /label=RSV/HIV-1\dU3/3'\LTR
                     /note="dU3: 1255-1308 / 3' LTR: 1309-1489"
     terminator      1565..1639
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1565..1639)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        6576..6822
                     /vntifkey="29"
                     /label=hU6\promoter
     misc_feature    6925..6940
                     /vntifkey="21"
                     /label=HIV-1\cPPT
     misc_feature    1243..1258
                     /vntifkey="21"
                     /label=HIV-1\cPPT
     promoter        1..352
                     /vntifkey="29"
                     /label=hPGK1\promoter
     misc_feature    6827..6874
                     /vntifkey="21"
                     /label=sh-EGFP
BASE COUNT     1814 a      1755 c      1866 g      1651 t 
ORIGIN
        1 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc 
       61 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc 
      121 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc 
      181 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac 
      241 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc 
      301 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag 
      361 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct 
      421 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct 
      481 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga 
      541 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 
      601 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 
      661 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 
      721 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 
      781 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 
      841 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 
      901 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 
      961 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 
     1021 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 
     1081 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc 
     1141 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag 
     1201 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 
     1261 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct 
     1321 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 
     1381 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 
     1441 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 
     1501 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 
     1561 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     1621 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     1681 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact 
     1741 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 
     1801 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 
     1861 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg 
     1921 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 
     1981 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 
     2041 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg 
     2101 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 
     2161 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 
     2221 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 
     2281 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 
     2341 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 
     2401 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 
     2461 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa 
     2521 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 
     2581 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 
     2641 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 
     2701 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 
     2761 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 
     2821 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 
     2881 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 
     2941 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 
     3001 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 
     3061 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 
     3121 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 
     3181 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 
     3241 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 
     3301 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 
     3361 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 
     3421 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 
     3481 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 
     3541 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 
     3601 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 
     3661 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 
     3721 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 
     3781 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 
     3841 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 
     3901 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 
     3961 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 
     4021 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 
     4081 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 
     4141 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 
     4201 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 
     4261 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 
     4321 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 
     4381 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 
     4441 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 
     4501 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 
     4561 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 
     4621 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 
     4681 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta 
     4741 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 
     4801 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 
     4861 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 
     4921 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 
     4981 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 
     5041 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 
     5101 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 
     5161 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 
     5221 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 
     5281 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 
     5341 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 
     5401 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 
     5461 aaacatcacg aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
     5521 tcagaagaac ttacatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
     5581 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
     5641 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga 
     5701 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     5761 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     5821 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct 
     5881 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     5941 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     6001 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 
     6061 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     6121 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     6181 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     6241 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     6301 ttggctgtgg tatataaaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 
     6361 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 
     6421 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 
     6481 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgat 
     6541 cacgagacta gcctcgagcg gccgccccct tcaccgaggg cctatttccc atgattcctt 
     6601 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa 
     6661 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg 
     6721 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt 
     6781 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccggtaca acagccacaa 
     6841 cgtctatctc gagatagacg ttgtggctgt tgtatttttg aattctcgac ctcgagacaa 
     6901 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg 
     6961 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt 
     7021 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg 
     7081 ctcgag 
//
