LOCUS       pCAGGS-E-hbeclin-1-C        5770 bp    DNA     circular     21-JUN-2012
DEFINITION  LMBP 7286.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7286
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 21-JUN-2012
COMMENT     VNTDATE|594919946|
COMMENT     VNTNAME|pCAGGS-E-hbeclin-1-C|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-hbeclin-1-C" 0 5770 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglII" "HincII" "KpnI" "NcoI" "NotI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5770 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BglII" "agatct" 2 0 2 120 0 1060 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HincII" "gtyrac" 4 0 1 4043 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 12 0 1803 0 4419 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "intron AG promoter" 15 0 0 4795 5719 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4796..5720") 
COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3047 3907 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3048..3908)") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 2105 2506 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2106..2507)") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1916 1990 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(1917..1991)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1916 1990 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1917..1991") 
COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 1810 1885 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1811..1886") 
COMMENT        #11=(CFSignal (CObList) "lac promoter" 30 0 1 1586 1668 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1587..1669)") 
COMMENT        #12=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 1060 1151 0 (CStringList) (CStringList) 1 1 1 1 "1061..1152") 
COMMENT        #13=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "16..54") 
COMMENT        #14=(CFSignal (CObList) "AG promoter" 29 0 0 4426 5767 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4427..5768") 
COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4043 4420 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4044..4421") 
COMMENT        #16=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 1152 1508 0 (CStringList) (CStringList) 1 1 1 1 "1153..1509") 
COMMENT        #17=(CFSignal (CObList) "hBECN1-C fragment" 4 0 0 64 972 0 (CStringList) 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "Last modified: 21-JUN-2012" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-hbeclin-1-C'" 1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 07/05/11 03:32" 1) 
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COMMENT                          "Last Modified: 06/21/12 10:03" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5770 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 8-AUG-2011" 1))) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
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COMMENT                                   #73=(CLinePar 
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COMMENT                                        "   4044..4421" 1))) "hCMV-IE enhancer") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                        "   complement(1587..1669)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     intron          4795..5719
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(3047..3907)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2105..2506)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(1916..1990)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      1916..1990
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      1810..1885
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1586..1668)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1060..1151
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        4426..5767
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4043..4420
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1152..1508
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..972
                     /vntifkey="4"
                     /label=hBECN1-C\fragment
BASE COUNT     1278 a      1468 c      1615 g      1409 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gcaatgactc agctcaacgt cactgaaaat gagtgtcaga actacaaacg ctgtttggag 
      121 atcttagagc aaatgaatga ggatgacagt gaacagttac agatggagct aaaggagctg 
      181 gcactagagg aggagaggct gatccaggag ctggaagacg tggaaaagaa ccgcaagata 
      241 gtggcagaaa atctcgagaa ggtccaggct gaggctgaga gactggatca ggaggaagct 
      301 cagtatcaga gagaatacag tgaatttaaa cgacagcagc tggagctgga tgatgagctg 
      361 aagagtgttg aaaaccagat gcgttatgcc cagacgcagc tggataagct gaagaaaacc 
      421 aacgtcttta atgcaacctt ccacatctgg cacagtggac agtttggcac aatcaataac 
      481 ttcaggctgg gtcgcctgcc cagtgttccc gtggaatgga atgagattaa tgctgcttgg 
      541 ggccagactg tgttgctgct ccatgctctg gccaataaga tgggtctgaa atttcagaga 
      601 taccgacttg ttccttacgg aaaccattca tatctggagt ctctgacaga caaatctaag 
      661 gagctgccgt tatactgttc tggggggttg cggtttttct gggacaacaa gtttgaccat 
      721 gcaatggtgg ctttcctgga ctgtgtgcag cagttcaaag aagaggttga gaaaggcgag 
      781 actcgttttt gtcttcccta caggatggat gtggagaaag gcaagattga agacacagga 
      841 ggcagtggcg gctcctattc catcaaaacc cagtttaact ctgaggagca gtggacaaaa 
      901 gctctcaagt tcatgctgac gaatcttaag tggggtcttg cttgggtgtc ctcacaattt 
      961 tataacaaat gagtcgagga attcactcct caggtgcagg ctgcctatca gaaggtggtg 
     1021 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa 
     1081 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt 
     1141 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg 
     1201 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc 
     1261 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag 
     1321 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt 
     1381 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta 
     1441 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta 
     1501 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt 
     1561 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 
     1621 agcctgggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
     1681 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag 
     1741 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     1801 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 
     1861 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt 
     1921 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     1981 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     2041 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 
     2101 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     2161 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag 
     2221 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 
     2281 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 
     2341 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 
     2401 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 
     2461 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 
     2521 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 
     2581 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 
     2641 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 
     2701 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc 
     2761 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 
     2821 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 
     2881 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 
     2941 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 
     3001 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 
     3061 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 
     3121 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 
     3181 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 
     3241 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 
     3301 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 
     3361 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 
     3421 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 
     3481 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 
     3541 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 
     3601 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 
     3661 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 
     3721 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 
     3781 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 
     3841 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 
     3901 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 
     3961 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 
     4021 cccgaaaagt gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt 
     4081 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 
     4141 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 
     4201 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 
     4261 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 
     4321 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 
     4381 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg 
     4441 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt 
     4501 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg 
     4561 gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag 
     4621 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa 
     4681 aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc 
     4741 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc 
     4801 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt 
     4861 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg 
     4921 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg 
     4981 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg 
     5041 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac 
     5101 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc 
     5161 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt 
     5221 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag 
     5281 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg 
     5341 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt 
     5401 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa 
     5461 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg 
     5521 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc 
     5581 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc 
     5641 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc 
     5701 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt 
     5761 tggcaaagaa 
//
