LOCUS       pCAGGS-E-hbeclin-1-a        6215 bp    DNA     circular      8-AUG-2011
DEFINITION  LMBP 7298.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7298
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 8-AUG-2011
            
            
            
            
            
            
            
COMMENT     VNTDATE|594924143|
COMMENT     VNTNAME|pCAGGS-E-hbeclin-1-a|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HincII" "gtyrac" 4 0 1 4488 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 12 0 387 0 2248 0 4864 0 
COMMENT            0 "") 
COMMENT        #4=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 128 0 1966 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3492 4352 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3492..4352)") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 2550 2951 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2550..2951)") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2361 2435 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2361..2435)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2361 2435 0 
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COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 2255 2330 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "lac promoter" 30 0 1 2031 2113 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
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COMMENT                          "Created: 07/05/11 04:42" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
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FEATURES             Location/Qualifiers
     intron          5240..6164
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(3492..4352)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(2550..2951)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(2361..2435)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      2361..2435
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      2255..2330
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(2031..2113)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      1505..1596
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        4871..6212
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        4488..4865
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    1597..1953
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..1416
                     /vntifkey="4"
                     /label=hBECN1
BASE COUNT     1400 a      1592 c      1730 g      1493 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctatggaag ggtctaagac gtccaacaac agcaccatgc aggtgagctt cgtgtgccag 
      121 cgctgcagcc agcccctgaa actggacacg agtttcaaga tcctggaccg tgtcaccatc 
      181 caggaactca cagctccatt acttaccaca gcccaggcga aaccaggaga gacccaggag 
      241 gaagagacta actcaggaga ggagccattt attgaaactc ctcgccagga tggtgtctct 
      301 cgcagattca tccccccagc caggatgatg tccacagaaa gtgccaacag cttcactctg 
      361 attggggagg catctgatgg cggcaccatg gagaacctca gccgaagact gaaggtcact 
      421 ggggaccttt ttgacatcat gtcgggccag acagatgtgg atcacccact ctgtgaggaa 
      481 tgcacagata ctcttttaga ccagctggac actcagctca acgtcactga aaatgagtgt 
      541 cagaactaca aacgctgttt ggagatctta gagcaaatga atgaggatga cagtgaacag 
      601 ttacagatgg agctaaagga gctggcacta gaggaggaaa ggctgatcca ggagctggaa 
      661 gacgtggaaa agaaccgcaa gatagtggca gaaaatctcg agaaggtcca ggctgaggct 
      721 gaaagactgg atcaggagga agctcagtat cagagagaat acagtgaatt taaacgacag 
      781 cagctggagc tggatgatga gctgaagagt gttgaaaacc agatgcgtta tgcccagacg 
      841 cagctggata agctgaagaa aaccaacgtc tttaatgcaa ccttccacat ctggcacagt 
      901 ggacagtttg gcacaatcaa taacttcagg ctgggtcgcc tgcccagtgt tcccgtggaa 
      961 tggaatgaga ttaatgctgc ttggggccag actgtgttgc tgctccatgc tctggccaat 
     1021 aagatgggtc tgaaatttca gagataccga cttgttcctt acggaaacca ttcatatctg 
     1081 gagtctctga cagacaaatc taaggagctg ccgttatact gttctggggg gttgcggttt 
     1141 ttctgggaca acaagtttga ccatgcaatg gtggctttcc tggactgtgt gcagcagttc 
     1201 aaagaagagg ttgagaaagg tgagacacgt ttttgtcttc cctacaggat ggatgtggag 
     1261 aaaggcaaga ttgaagacac aggaggcagt ggcggctcct attccatcaa aacccagttt 
     1321 aactctgagg agcagtggac aaaagctctc aagttcatgc tgacgaatct taagtggggt 
     1381 cttgcttggg tgtcctcaca attttataac aaatgaggtc gaggaattca ctcctcaggt 
     1441 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca 
     1501 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct 
     1561 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg 
     1621 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt 
     1681 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata 
     1741 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc 
     1801 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc 
     1861 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc 
     1921 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt 
     1981 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
     2041 tacgagccgg aagcataaag tgtaaagcct gggtgcctaa tgagtgagct aactcacatt 
     2101 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccg 
     2161 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 
     2221 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 
     2281 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 
     2341 ctaggctttt gcaaaaagct aacttgttta ttgcagctta taatggttac aaataaagca 
     2401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     2461 ccaaactcat caatgtatct tatcatgtct ggatccgctg cattaatgaa tcggccaacg 
     2521 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     2581 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     2641 atccacagaa tcagggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     2701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     2761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     2821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     2881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     2941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     3001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     3061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     3121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 
     3181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     3241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     3301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     3361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 
     3421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     3481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     3541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     3601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     3661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     3721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     3781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 
     3841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     3901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     3961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     4021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     4081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 
     4141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 
     4201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 
     4261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 
     4321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 
     4381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 
     4441 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt 
     4501 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt 
     4561 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc 
     4621 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 
     4681 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat 
     4741 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca 
     4801 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat 
     4861 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 
     4921 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 
     4981 gggggggggg ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg 
     5041 gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag 
     5101 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg 
     5161 ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact 
     5221 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta 
     5281 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct 
     5341 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt 
     5401 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc 
     5461 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg 
     5521 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg 
     5581 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt 
     5641 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc 
     5701 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc 
     5761 cggggagggc tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg 
     5821 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt 
     5881 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc 
     5941 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt 
     6001 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct 
     6061 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta 
     6121 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc 
     6181 tggttattgt gctgtctcat cattttggca aagaa 
//
