LOCUS       pCAGGS-E-hbeclin-1-N        5263 bp    DNA     circular     21-JUN-2012
DEFINITION  LMBP 7299.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7299
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 21-JUN-2012
            
COMMENT     VNTDATE|594922620|
COMMENT     VNTNAME|pCAGGS-E-hbeclin-1-N|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-hbeclin-1-N" 0 5263 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "HindIII" "KpnI" "NcoI" "NotI" "PstI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 1 0 0 0 0 1 5263 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1019 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 12 0 387 0 1296 0 3912 0 
COMMENT            0 "") #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 57 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 128 0 1014 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #6=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2540 3400 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2540..3400)") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 1598 1999 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1598..1999)") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1409 1483 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(1409..1483)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1409 1483 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1409..1483") 
COMMENT        #10=(CFSignal (CObList) "SV40 ori" 33 0 0 1303 1378 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1303..1378") 
COMMENT        #11=(CFSignal (CObList) "lac promoter" 30 0 1 1079 1161 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT             0 553 644 0 (CStringList) (CStringList) 1 1 1 1 "553..644") 
COMMENT        #13=(CFSignal (CObList) "E-tag" 22 0 0 16 54 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "AG promoter" 29 0 0 3919 5260 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3919..5260") 
COMMENT        #15=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3536 3913 0 
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COMMENT        #16=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
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COMMENT        #17=(CFSignal (CObList) "hBECN1-N fragment" 4 0 0 64 465 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "LMBP 7299" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-hbeclin-1-N'" 1) 
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COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 07/05/11 04:17" 1) 
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COMMENT                          "Last Modified: 06/21/12 09:28" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.lmbp@UGent.be</a>" 
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FEATURES             Location/Qualifiers
     intron          4288..5212
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(2540..3400)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(1598..1999)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(1409..1483)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      1409..1483
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      1303..1378
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1079..1161)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      553..644
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_marker     16..54
                     /vntifkey="22"
                     /label=E-tag
     promoter        3919..5260
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     promoter        3536..3913
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     misc_feature    645..1001
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     CDS             64..465
                     /vntifkey="4"
                     /label=hBECN1-N\fragment
BASE COUNT     1120 a      1403 c      1467 g      1273 t 
ORIGIN
        1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc 
       61 gctatggaag ggtctaagac gtccaacaac agcaccatgc aggtgagctt cgtgtgccag 
      121 cgctgcagcc agcccctgaa actggacacg agtttcaaga tcctggaccg tgtcaccatc 
      181 caggaactca cagctccatt acttaccaca gcccaggcga aaccaggaga gacccaggag 
      241 gaagagacta actcaggaga ggagccattt attgaaactc ctcgccagga tggtgtctct 
      301 cgcagattca tccccccagc caggatgatg tccacagaaa gtgccaacag cttcactctg 
      361 attggggagg catctgatgg cggcaccatg gagaacctca gccgaagact gaaggtcact 
      421 ggggaccttt ttgacatcat gtcgggccag acagatgtgg attgagtcga ggaattcact 
      481 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac 
      541 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt 
      601 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat 
      661 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat 
      721 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt 
      781 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat 
      841 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct 
      901 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga 
      961 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag 
     1021 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 
     1081 acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg agtgagctaa 
     1141 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     1201 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 
     1261 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 
     1321 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 
     1381 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa 
     1441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     1501 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc 
     1561 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     1621 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     1681 atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca aaaggccagc 
     1741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     1801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     1861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     1921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     1981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     2041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     2101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     2161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     2221 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     2281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     2341 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     2401 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     2461 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     2521 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     2581 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     2641 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     2701 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     2761 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     2821 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     2881 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     2941 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     3001 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     3061 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     3121 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     3181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     3241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     3301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     3361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     3421 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     3481 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgacat 
     3541 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat 
     3601 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
     3661 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
     3721 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 
     3781 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
     3841 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
     3901 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc 
     3961 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg 
     4021 gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg gcggggcggg 
     4081 gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt 
     4141 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc 
     4201 gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg 
     4261 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc 
     4321 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt 
     4381 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt 
     4441 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg 
     4501 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt 
     4561 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg 
     4621 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct 
     4681 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg 
     4741 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg 
     4801 cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg ccggcggctg 
     4861 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg 
     4921 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc 
     4981 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct 
     5041 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg 
     5101 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg 
     5161 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg 
     5221 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa 
//
