LOCUS       pCD-F-bio-Bcl10         6150 bp    DNA     circular     22-JUN-2011
DEFINITION  LMBP 7362.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|593615193|
COMMENT     VNTDBDATE|593615337|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCD-F-bio-Bcl10|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
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COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 1076 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 5432 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 5343 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 962 0 1697 0 5042 0 5350 
COMMENT            0 5443 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1318 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 1078 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 1076 0 0 "")) 
COMMENT       (CObList 
COMMENT        #10=(CFSignal (CObList) "flag epitope" 21 0 0 5351 5377 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "920..946") 
COMMENT        #11=(CFSignal (CObList) "AmpR" 22 0 1 3435 4296 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4677..5538)") 
COMMENT        #12=(CFSignal (CObList) "ColE1" 33 0 0 2617 3290 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3859..4532") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA" 25 0 0 1946 2185 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3188..3427") 
COMMENT        #14=(CFSignal (CObList) "NeoR" 86 0 0 1136 1930 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2378..3172") 
COMMENT        #15=(CFSignal (CObList) "SV40" 29 0 0 775 1100 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2017..2342") 
COMMENT        #16=(CFSignal (CObList) "f1" 33 0 0 297 710 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1539..1952") 
COMMENT        #17=(CFSignal (CObList) "BGH polyA" 25 0 0 20 234 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1262..1476") 
COMMENT        #18=(CFSignal (CObList) "T7" 30 0 0 5294 5313 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "863..882") 
COMMENT        #19=(CFSignal (CObList) "pCMV" 29 0 0 4640 5294 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "209..863") 
COMMENT        #20=(CFSignal (CObList) "hBcl-10" 4 0 0 5444 6145 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "968..1669") 
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FEATURES             Location/Qualifiers
     misc_feature    5351..5377
                     /vntifkey="21"
                     /label=flag\epitope
     misc_marker     complement(3435..4296)
                     /vntifkey="22"
                     /label=AmpR
     rep_origin      2617..3290
                     /vntifkey="33"
                     /label=ColE1
     polyA_signal    1946..2185
                     /vntifkey="25"
                     /label=SV40\polyA
     misc_recomb     1136..1930
                     /vntifkey="86"
                     /label=NeoR
     promoter        775..1100
                     /vntifkey="29"
                     /label=SV40
     rep_origin      297..710
                     /vntifkey="33"
                     /label=f1
     polyA_signal    20..234
                     /vntifkey="25"
                     /label=BGH\polyA
     promoter        5294..5313
                     /vntifkey="30"
                     /label=T7
     promoter        4640..5294
                     /vntifkey="29"
                     /label=pCMV
     CDS             5444..6145
                     /vntifkey="4"
                     /label=hBcl-10
     CDS             5378..5437
                     /vntifkey="4"
                     /label=bio-tag
BASE COUNT     1500 a      1569 c      1533 g      1548 t 
ORIGIN
        1 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 
       61 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 
      121 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 
      181 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 
      241 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 
      301 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 
      361 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 
      421 cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc 
      481 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 
      541 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 
      601 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 
      661 gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 
      721 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc 
      781 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc 
      841 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt 
      901 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc 
      961 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct 
     1021 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg 
     1081 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat 
     1141 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 
     1201 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 
     1261 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 
     1321 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 
     1381 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 
     1441 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 
     1501 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 
     1561 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 
     1621 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 
     1681 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 
     1741 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 
     1801 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 
     1861 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 
     1921 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca 
     1981 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 
     2041 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 
     2101 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 
     2161 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 
     2221 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag 
     2281 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 
     2341 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 
     2401 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 
     2461 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 
     2521 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     2581 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
     2641 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
     2701 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
     2761 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
     2821 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc 
     2881 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
     2941 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
     3001 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
     3061 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
     3121 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
     3181 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
     3241 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
     3301 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
     3361 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
     3421 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
     3481 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
     3541 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
     3601 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
     3661 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
     3721 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 
     3781 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
     3841 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
     3901 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
     3961 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
     4021 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 
     4081 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
     4141 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
     4201 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
     4261 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
     4321 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
     4381 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg 
     4441 ggagatctcc cgatccccta tggtcgactc tcagtacaat ctgctctgat gccgcatagt 
     4501 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa 
     4561 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta 
     4621 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga 
     4681 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc 
     4741 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat 
     4801 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc 
     4861 aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc 
     4921 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt 
     4981 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta 
     5041 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg 
     5101 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac 
     5161 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg 
     5221 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact 
     5281 ggcttatcga aattaatacg actcactata gggagaccca agctggctag cgtttaaact 
     5341 taagcttacc atggattaca aggacgatga cgataaggta ccaggcctga acgacatctt 
     5401 cgaggcccag aagatcgagt ggcacgagga ggatcctgcc accatggagc ccaccgcacc 
     5461 gtccctcacc gaggaggacc tcactgaagt gaagaaggac gccttagaaa atttacgtgt 
     5521 atacctgtgt gagaaaatca tagctgagag acattttgat catctacgtg caaaaaaaat 
     5581 actcagtaga gaagacactg aagaaatttc ttgtcgaaca tcaagtagaa aaagggctgg 
     5641 aaaattgtta gactacttac aggaaaaccc aaaaggtctg gacacccttg ttgaatctat 
     5701 tcggcgagaa aaaacacaga acttcctgat acagaagatt acagatgaag tgctgaaact 
     5761 tagaaatata aaactagaac atctgaaagg actaaaatgt agcagttgtg aaccttttcc 
     5821 agatggagcc acgaacaacc tctccagatc aaattcagat gagagtaatt tctctgaaaa 
     5881 actgagggca tccactgtca tgtaccatcc agaaggagaa tccagcacga cgcccttttt 
     5941 ttctactaat tcttctctga atttgcctgt tctagaagta ggcagaactg aaaataccat 
     6001 cttctcttca actacacttc ccagacctgg ggacccaggg gctcctcctt tgccaccaga 
     6061 tctacagtta gaagaagaag gaacttgtgc aaactctagt gagatgtttc ttcccttaag 
     6121 atcacgtact gtttcacgac aatgagggcc 
//
