LOCUS       pCAGGS-E-ASC            5363 bp    DNA     circular      2-NOV-2012
DEFINITION  LMBP 7414.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7414
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 2-NOV-2012
                        
COMMENT     VNTDATE|641645269|
COMMENT     VNTNAME|pCAGGS-E-ASC|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-ASC" 0 5363 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglII" "EcoRI" "HincII" "NcoI" "SacII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5363 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BglII" "agatct" 2 0 1 656 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 7 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HincII" "gtyrac" 4 0 1 3639 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 15 0 204 0 1399 0 4015 0 
COMMENT            0 "") 
COMMENT        #4=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 3 188 0 4671 0 5200 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "SV40 ori" 33 0 0 1406 1481 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1664..1739") 
COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 1 1182 1264 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1440..1522)") 
COMMENT        #7=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT            0 656 747 0 (CStringList) (CStringList) 1 1 1 1 "914..1005") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 1701 2102 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1959..2360)") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1512 1586 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1770..1844") 
COMMENT        #10=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1512 1586 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1770..1844)") 
COMMENT        #11=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 
COMMENT             0 748 1104 0 (CStringList) (CStringList) 1 1 1 1 "1006..1362") 
COMMENT        #12=(CFSignal (CObList) "AG promoter" 29 0 0 4022 5363 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4280..5621") 
COMMENT        #13=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 3639 4016 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3897..4274") 
COMMENT        #14=(CFSignal (CObList) "intron AG promoter" 15 0 0 4391 5315 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4649..5573") 
COMMENT        #15=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2643 3503 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2901..3761)") 
COMMENT        #16=(CFSignal (CObList) "E-tag" 22 0 0 19 57 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "hPYCARD" 4 0 0 67 654 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "LMBP 7414" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-E-ASC'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 11/02/12 10:47" 1) 
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COMMENT                          "Last Modified: 11/02/12 11:07" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5363 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 14-DEC-2011" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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FEATURES             Location/Qualifiers
     rep_origin      1406..1481
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1182..1264)
                     /vntifkey="30"
                     /label=lac\promoter
     terminator      656..747
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     rep_origin      complement(1701..2102)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      1512..1586
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1512..1586)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     misc_feature    748..1104
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        4022..5363
                     /vntifkey="29"
                     /label=AG\promoter
     promoter        3639..4016
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     intron          4391..5315
                     /vntifkey="15"
                     /label=intron\AG\promoter
     CDS             complement(2643..3503)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_marker     19..57
                     /vntifkey="22"
                     /label=E-tag
     CDS             67..654
                     /vntifkey="4"
                     /label=hPYCARD
BASE COUNT     1105 a      1457 c      1529 g      1272 t 
ORIGIN
        1 gaattgaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg 
       61 gccgctatgg ggcgcgcgcg cgacgccatc ctggatgcgc tggagaacct gaccgccgag 
      121 gagctcaaga agttcaagct gaagctgctg tcggtgccgc tgcgcgaggg ctacgggcgc 
      181 atcccgcggg gcgcgctgct gtccatggac gccttggacc tcaccgacaa gctggtcagc 
      241 ttctacctgg agacctacgg cgccgagctc accgctaacg tgctgcgcga catgggcctg 
      301 caggagatgg ccgggcagct gcaggcggcc acgcaccagg gctctggagc cgcgccagct 
      361 gggatccagg cccctcctca gtcggcagcc aagccaggcc tgcactttat agaccagcac 
      421 cgggctgcgc ttatcgcgag ggtcacaaac gttgagtggc tgctggatgc tctgtacggg 
      481 aaggtcctga cggatgagca gtaccaggca gtgcgggccg agcccaccaa cccaagcaag 
      541 atgcggaagc tcttcagttt cacaccagcc tggaactgga cctgcaagga cttgctcctc 
      601 caggccctaa gggagtccca gtcctacctg gtggaggacc tggagcggag ctgaagatct 
      661 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 
      721 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 
      781 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag 
      841 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca 
      901 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg 
      961 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt 
     1021 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc 
     1081 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt 
     1141 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     1201 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt 
     1261 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat 
     1321 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
     1381 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
     1441 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 
     1501 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     1561 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     1621 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag 
     1681 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     1741 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     1801 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     1861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     1921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     1981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     2041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     2101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     2161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     2221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     2281 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 
     2341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     2401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     2461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     2521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     2581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     2641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     2701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     2761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     2821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     2881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     2941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     3001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     3061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     3121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     3181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     3241 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     3301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     3361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     3421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     3481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     3541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     3601 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta 
     3661 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 
     3721 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 
     3781 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 
     3841 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac 
     3901 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 
     3961 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt 
     4021 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat 
     4081 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg 
     4141 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc 
     4201 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg 
     4261 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc 
     4321 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt 
     4381 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt 
     4441 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg 
     4501 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc 
     4561 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg 
     4621 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg 
     4681 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg 
     4741 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca 
     4801 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg 
     4861 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg 
     4921 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc 
     4981 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa 
     5041 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga 
     5101 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc 
     5161 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg 
     5221 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg 
     5281 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg 
     5341 tgctgtctca tcattttggc aaa 
//
