LOCUS       pCAGGS-E-hCASP-1CARD        5158 bp    DNA     circular      3-OCT-2011
DEFINITION  LMBP 7418.
SOURCE      
  ORGANISM  
COMMENT     (800)357-3114 or http://www.informaxinc.com/
COMMENT     VNTDATE|602597018|
COMMENT     VNTNAME|pCAGGS-E-hCASP-1CARD|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-E-hCASP-1CARD" 0 5158 
COMMENT       (CDBMol 0 0 1 4784 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 1 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 5 40 150 0 1 0 
COMMENT        (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "BglII" "NotI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5158 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BglII" "agatct" 2 0 1 1 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 4785 0 0 "")) 
COMMENT       (CObList 
COMMENT        #2=(CFSignal (CObList) "ETAG" 21 0 0 4737 4787 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4737..4787") 
COMMENT        #3=(CFSignal (CObList) "E-Casp1 CARD WT" 4 0 0 4741 5154 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "AmpR" 4 0 1 2008 2865 0 (CStringList) 
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COMMENT                  "pCAGGS-E-hCASP-1CARD" 2) 5 "" 0 4) 
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COMMENT                  (CObjectList 
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COMMENT                       "DNA Plasmid 'pCAGGS-E-hCASP-1CARD'" 1) 
COMMENT                   #9=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                       "INCA was inserted as a NotI-Bgl II fragment in pCAGGS-Etag-mCasp1" 
COMMENT                       1) 
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COMMENT                        "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                   #11=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "Created: 09/30/11 12:03" 1) 
COMMENT                   #12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "Last Modified: 10/03/11 10:19" 1) 
COMMENT                   #13=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "length: 5158 bp" 1) 
COMMENT                   #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                        "storage type: Basic" 1) 
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COMMENT                                       "Start: 2008  End: 2865 (Complementary)" 
COMMENT                                       1))) "AmpR") 
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COMMENT                                "E-Casp1 CARD WT"))) "CDS (2 total)") 
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COMMENT                                       "Start: 4737  End: 4787" 1) 
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COMMENT                                       "E-tag" 1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                       " N1: 1 " 1))))) "BglII: 1 site") 1 5 
COMMENT                         "AGATCT" "TCTAGA") 
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COMMENT                                       " N1: 4785 " 1))))) "NotI: 1 site") 2 6 
COMMENT                         "GCGGCCGC" "CGCCGGCG"))) 
COMMENT                  "Restriction/Methylation Map:  (no more than 1 site)")))) 
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FEATURES             Location/Qualifiers
     misc_feature    4737..4787
                     /vntifkey="21"
                     /label=ETAG
                     /note="E-tag"
     CDS             4741..5154
                     /vntifkey="4"
                     /label=E-Casp1\CARD\WT
     CDS             complement(2008..2865)
                     /vntifkey="4"
                     /label=AmpR
BASE COUNT     1112 a      1331 c      1424 g      1261 t        30 others
ORIGIN
        1 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact 
       61 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc 
      121 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt 
      181 tagagtttgg caacatatgc catatgctgg ctgccatgaa caaaggtggc tataaagagg 
      241 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac 
      301 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa 
      361 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat 
      421 agctgtccct cttctcttat gaagatcnnn nctcgacctg cagcccaagc ttggcgtaat 
      481 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 
      541 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 
      601 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag nnnnnnnnnn 
      661 nggatccnat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 
      721 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 
      781 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct 
      841 tttttggagg cctaggcttt tgcaaaaagc tnaacttgtt tattgcagct tataatggtt 
      901 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
      961 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccnc tgcattaatg 
     1021 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 
     1081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
     1141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
     1201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
     1261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     1321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     1381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     1441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     1501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     1561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     1621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     1681 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     1741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     1801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     1861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     1921 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     1981 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     2041 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     2101 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     2161 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     2221 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     2281 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 
     2341 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     2401 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     2461 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     2521 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     2581 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 
     2641 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     2701 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     2761 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     2821 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     2881 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     2941 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgnnnn 
     3001 ngtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat 
     3061 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 
     3121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 
     3181 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta 
     3241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 
     3301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 
     3361 gtattagtca tcgctattac catggnnnnn gtcgaggtga gccccacgtt ctgcttcact 
     3421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt 
     3481 tgtgcagcga tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga 
     3541 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg 
     3601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg 
     3661 gcgggcggga gtcgctgcgt tgccttcgcc ccgtgccccg ctccgcgccg cctcgcgccg 
     3721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 
     3781 tcctccgggc tgtaattagc gcttggttta atgacggctc gtttcttttc tgtggctgcg 
     3841 tgaaagcctt aaagggctcc gggagggccc tttgtgcggg ggggagcggc tcggggggtg 
     3901 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggccc gcgctgcccg gcggctgtga 
     3961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcgtg tgcgcgaggg gagcgcggcc 
     4021 gggggcggtg ccccgcggtg cgggggggct gcgaggggaa caaaggctgc gtgcggggtg 
     4081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcggcggt cgggctgtaa cccccccctg 
     4141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtgcgggg 
     4201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg 
     4261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc cggagcgccg 
     4321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg 
     4381 cgcagggact tcctttgtcc caaatctggc ggagccgaaa tctgggaggc gccgccgcac 
     4441 cccctctagc gggcgcgggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag 
     4501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccatc tccagcctcg gggctgccgc 
     4561 agggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga 
     4621 ccggcgggnt ctagancctc tgctaaccat gttcatgcct tcttcttttt cctacagctc 
     4681 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt gaattccacc 
     4741 atgggtgcgc cggtgccgta tccggacccg ctggaaccgc gtgcggccgc tgataccatg 
     4801 gccgacaagg tcctgaagga gaagagaaag ctgtttatcc gttccatggg tgaaggtaca 
     4861 ataaatggct tactggatga attattacag acaagggtgc tgaacaagga agagatggag 
     4921 aaagtaaaac gtgaaaatgc tacagttatg gataagaccc gagctttgat tgactccgtt 
     4981 attccgaaag gggcacaggc atgccaaatt tgcatcacat acatttgtga agaagacagt 
     5041 tacctggcag ggacgctggg actctcagca gatcaaacat ctggaaatta ccttaatatg 
     5101 caagactctc aaggagtact ttcttccttt ccagctcctc aggcagtgca ggactaaa 
//


