ID pCAGGS-FLAG-hCASP-1-C285A standard; circular DNA; ; 5969 BP. DE LMBP 7424. CC Accession number: LMBP 7424 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 6-FEB-2015 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator 1262..1353 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT misc_feature 1354..1710 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT promoter complement(1788..1870) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin 2012..2087 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 2118..2192 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(2118..2192) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(2307..2708) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(3249..4109) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter 4245..4622 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="human CMV-IE enhancer" FT promoter 4628..5969 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 4997..5921 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid intron" FT misc_marker 13..39 FT /vntifkey="22" FT /label=FLAG\epitope FT CDS 46..1260 FT /vntifkey="4" FT /label=hCASP1\mutant FT mutation 898..900 FT /vntifkey="62" FT /label=C285A FT /note="WT: TGC" SQ Sequence 5969 BP; 1369 A; 1516 C; 1610 G; 1474 t; gaattcctcg agatggacta caaggacgac gatgacaagg gtaccatggc cgacaaggtc 60 ctgaaggaga agagaaagct gtttatccgt tccatgggtg aaggtacaat aaatggctta 120 ctggatgaat tattacagac aagggtgctg aacaaggaag agatggagaa agtaaaacgt 180 gaaaatgcta cagttatgga taagacccga gctttgattg actccgttat tccgaaaggg 240 gcacaggcat gccaaatttg catcacatac atttgtgaag aagacagtta cctggcaggg 300 acgctgggac tctcagcaga tcaaacatct ggaaattacc ttaatatgca agactctcaa 360 ggagtacttt cttcctttcc agctcctcag gcagtgcagg acaacccagc tatgcccaca 420 tcctcaggct cagaagggaa tgtcaagctt tgctccctag aagaagctca aaggatatgg 480 aaacaaaagt cggcagagat ttatccaata atggacaagt caagccgcac acgtcttgct 540 ctcattatct gcaatgaaga atttgacagt attcctagaa gaactggagc tgaggttgac 600 atcacaggca tgacaatgct gctacaaaat ctggggtaca gcgtagatgt gaaaaaaaat 660 ctcactgctt cggacatgac tacagagctg gaggcatttg cacaccgccc agagcacaag 720 acctctgaca gcacgttcct ggtgttcatg tctcatggta ttcgggaagg catttgtggg 780 aagaaacact ctgagcaagt cccagatata ctacaactca atgcaatctt taacatgttg 840 aataccaaga actgcccaag tttgaaggac aaaccgaagg tgatcatcat ccaggccgcc 900 cgtggtgaca gccctggtgt ggtgtggttt aaagattcag taggagtttc tggaaaccta 960 tctttaccaa ctacagaaga gtttgaggat gatgctatta agaaagccca catagagaag 1020 gattttatcg ctttctgctc ttccacacca gataatgttt cttggagaca tcccacaatg 1080 ggctctgttt ttattggaag actcattgaa catatgcaag aatatgcctg ttcctgtgat 1140 gtggaggaaa ttttccgcaa ggttcgattt tcatttgagc agccagatgg tagagcgcag 1200 atgcccacca ctgaaagagt gactttgaca agatgtttct acctcttccc aggacattaa 1260 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac 1320 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct 1380 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt 1440 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag 1500 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt 1560 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct 1620 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt 1680 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat 1740 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 1800 gagccggaag cataaagtgt aaagcctggg tgcctaatga gtgagctaac tcacattaat 1860 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat 1920 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 1980 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 2040 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 2100 ggcttttgca aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata 2160 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 2220 aactcatcaa tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc 2280 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 2340 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 2400 cacagaatca gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 2460 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 2520 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 2580 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 2640 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 2700 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 2760 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 2820 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 2880 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 2940 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 3000 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 3060 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 3120 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 3180 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 3240 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 3300 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 3360 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 3420 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 3480 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 3540 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 3600 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 3660 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 3720 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 3780 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 3840 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 3900 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 3960 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 4020 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 4080 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 4140 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 4200 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac 4260 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 4320 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 4380 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 4440 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 4500 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 4560 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 4620 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc 4680 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg 4740 gggggggccc cccccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggaa 4800 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg 4860 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg 4920 ccttcccccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac 4980 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg 5040 cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg aggggctccg 5100 ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg 5160 gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg 5220 gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg 5280 cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc 5340 agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc cccgagttgc 5400 tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt 5460 gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc ctcgggccgg 5520 ggagggctcg ggggaagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg 5580 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt 5640 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg 5700 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc 5760 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc 5820 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag 5880 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg 5940 ttattgtgct gtctcatcat tttggcaaa 5969 //