LOCUS       pCAGGS-E-hCASP-1p30      5633 bp    DNA     linear   UNA dsDNACircular
DEFINITION  LMBP 7661.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     Misc_marker     11..50
                     /note="E-tag"
                     /label=E-tag
     CDS             59..916
                     /note="human caspase-1p30 coding sequence"
                     /label=hCASP-1p30
     Terminator      918..1009
                     /note="3' UTR of the rabbit ß-globin gene, incl. polyA"
                     /label="ß-globin polyA"
     Misc_feature    1010..1366
                     /note="3' flanking region of the rabbit ß-globin gene"
                     /label="3FR of ß-globin"
     Promoter        complement(1444..1526)
                     /note="E. coli lac operon promoter"
                     /label="lac promoter"
     Replication_ori 1668..1743
                     /note="bidirectional origin of SV40"
                     /label="SV40 ori"
     Terminator      1774..1848
                     /note="polyA signal of the SV40 early region"
                     /label="SV40 polyA early"
     Terminator      complement(1774..1848)
                     /note="polyA signal of the SV40 late region"
                     /label="SV40 polyA late"
     Replication_ori complement(1963..2364)
                     /note="origin plasmid"
                     /label="pMB1 ori"
     CDS             complement(2905..3765)
                     /note="antibiotic resistance gene; coding sequence"
                     /label=AMP
     Promoter        3901..4278
                     /note="human CMV-IE enhancer"
                     /label="hCMV enhancer"
     Promoter        4284..5625
                     /note="chicken ß-actin/ rabbit ß-globin hybrid promoter"
                     /label="AG promoter"
     Intron          4653..5577
                     /note="chicken ß-actin/ rabbit ß-globin hybrid intron"
                     /label="intron AG promoter"
ORIGIN
        1 TTCCACCATG GGTGCGCCGG TGCCGTATCC GGACCCGCTG GAACCGCGTG CGGCCGCTAA
       61 CCCAGCTATG CCCACATCCT CAGGCTCAGA AGGGAATGTC AAGCTTTGCT CCCTAGAAGA
      121 AGCTCAAAGG ATATGGAAAC AAAAGTCGGC AGAGATTTAT CCAATAATGG ACAAGTCAAG
      181 CCGCACACGT CTTGCTCTCA TTATCTGCAA TGAAGAATTT GACAGTATTC CTAGAAGAAC
      241 TGGAGCTGAG GTTGACATCA CAGGCATGAC AATGCTGCTA CAAAATCTGG GGTACAGCGT
      301 AGATGTGAAA AAAAATCTCA CTGCTTCGGA CATGACTACA GAGCTGGAGG CATTTGCACA
      361 CCGCCCAGAG CACAAGACCT CTGACAGCAC GTTCCTGGTG TTCATGTCTC ATGGTATTCG
      421 GGAAGGCATT TGTGGGAAGA AACACTCTGA GCAAGTCCCA GATATACTAC AACTCAATGC
      481 AATCTTTAAC ATGTTGAATA CCAAGAACTG CCCAAGTTTG AAGGACAAAC CGAAGGTGAT
      541 CATCATCCAG GCCTGCCGTG GTGACAGCCC TGGTGTGGTG TGGTTTAAAG ATTCAGTAGG
      601 AGTTTCTGGA AACCTATCTT TACCAACTAC AGAAGAGTTT GAGGATGATG CTATTAAGAA
      661 AGCCCACATA GAGAAGGATT TTATCGCTTT CTGCTCTTCC ACACCAGATA ATGTTTCTTG
      721 GAGACATCCC ACAATGGGCT CTGTTTTTAT TGGAAGACTC ATTGAACATA TGCAAGAATA
      781 TGCCTGTTCC TGTGATGTGG AGGAAATTTT CCGCAAGGTT CGATTTTCAT TTGAGCAGCC
      841 AGATGGTAGA GCGCAGATGC CCACCACTGA AAGAGTGACT TTGACAAGAT GTTTCTACCT
      901 CTTCCCAGGA CATTAAAGAT CTTTTTCCCT CTGCCAAAAA TTATGGGGAC ATCATGAAGC
      961 CCCTTGAGCA TCTGACTTCT GGCTAATAAA GGAAATTTAT TTTCATTGCA ATAGTGTGTT
     1021 GGAATTTTTT GTGTCTCTCA CTCGGAAGGA CATATGGGAG GGCAAATCAT TTAAAACATC
     1081 AGAATGAGTA TTTGGTTTAG AGTTTGGCAA CATATGCCCA TATGCTGGCT GCCATGAACA
     1141 AAGGTTGGCT ATAAAGAGGT CATCAGTATA TGAAACAGCC CCCTGCTGTC CATTCCTTAT
     1201 TCCATAGAAA AGCCTTGACT TGAGGTTAGA TTTTTTTTAT ATTTTGTTTT GTGTTATTTT
     1261 TTTCTTTAAC ATCCCTAAAA TTTTCCTTAC ATGTTTTACT AGCCAGATTT TTCCTCCTCT
     1321 CCTGACTACT CCCAGTCATA GCTGTCCCTC TTCTCTTATG GAGATCCCTC GACCTGCAGC
     1381 CCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA
     1441 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGTGCC TAATGAGTGA
     1501 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT
     1561 GCCAGCGGAT CCGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT
     1621 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT
     1681 TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG
     1741 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT TTATTGCAGC TTATAATGGT
     1801 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT
     1861 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCG CTGCATTAAT
     1921 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC
     1981 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG
     2041 CGGTAATACG GTTATCCACA GAATCAGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
     2101 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
     2161 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     2221 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     2281 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     2341 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     2401 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     2461 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     2521 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     2581 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     2641 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     2701 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
     2761 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     2821 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     2881 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     2941 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     3001 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
     3061 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC
     3121 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG
     3181 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG
     3241 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG
     3301 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG
     3361 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT
     3421 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA
     3481 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC
     3541 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC
     3601 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC
     3661 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC
     3721 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA
     3781 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT
     3841 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGGGTC
     3901 GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC
     3961 CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA
     4021 ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA
     4081 CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC
     4141 AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT
     4201 GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT
     4261 TAGTCATCGC TATTACCATG GTCGAGGTGA GCCCCACGTT CTGCTTCACT CTCCCCATCT
     4321 CCCCCCCCTC CCCACCCCCA ATTTTGTATT TATTTATTTT TTAATTATTT TGTGCAGCGA
     4381 TGGGGGCGGG GGGGGGGGGG GGGCCCCCCC CAGGCGGGGC GGGGCGGGGC GAGGGGCGGG
     4441 GCGGGGCGAG GCGGAAAGGT GCGGCGGCAG CCAATCAGAG CGGCGCGCTC CGAAAGTTTC
     4501 CTTTTATGGC GAGGCGGCGG CGGCGGCGGC CCTATAAAAA GCGAAGCGCG CGGCGGGCGG
     4561 GAGTCGTTGC GCGCTGCCTT CCCCCCGTGC CCCGCTCCGC CGCCGCCTCG CGCCGCCCGC
     4621 CCCGGCTCTG ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC CCTTCTCCTC
     4681 CGGGCTGTAA TTAGCGCTTG GTTTAATGAC GGCTTGTTTC TTTTCTGTGG CTGCGTGAAA
     4741 GCCTTGAGGG GCTCCGGGAG GGCCCTTTGT GCGGGGGGAG CGGCTCGGGG GGTGCGTGCG
     4801 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCTCCGCGCT GCCCGGCGGC TGTGAGCGCT
     4861 GCGGGCGCGG CGCGGGGCTT TGTGCGCTCC GCAGTGTGCG CGAGGGGAGC GCGGCCGGGG
     4921 GCGGTGCCCC GCGGTGCGGG GGGGGCTGCG AGGGGAACAA AGGCTGCGTG CGGGGTGTGT
     4981 GCGTGGGGGG GTGAGCAGGG GGTGTGGGCG CGTCGGTCGG GCTGCAACCC CCCCTGCACC
     5041 CCCCTCCCCG AGTTGCTGAG CACGGCCCGG CTTCGGGTGC GGGGCTCCGT ACGGGGCGTG
     5101 GCGCGGGGCT CGCCGTGCCG GGCGGGGGGT GGCGGCAGGT GGGGGTGCCG GGCGGGGCGG
     5161 GGCCGCCTCG GGCCGGGGAG GGCTCGGGGG AAGGGGCGCG GCGGCCCCCG GAGCGCCGGC
     5221 GGCTGTCGAG GCGCGGCGAG CCGCAGCCAT TGCCTTTTAT GGTAATCGTG CGAGAGGGCG
     5281 CAGGGACTTC CTTTGTCCCA AATCTGTGCG GAGCCGAAAT CTGGGAGGCG CCGCCGCACC
     5341 CCCTCTAGCG GGCGCGGGGC GAAGCGGTGC GGCGCCGGCA GGAAGGAAAT GGGCGGGGAG
     5401 GGCCTTCGTG CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC TCCAGCCTCG GGGCTGTCCG
     5461 CGGGGGGACG GCTGCCTTCG GGGGGGACGG GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG
     5521 ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC
     5581 CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT GAA
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