LOCUS       BH20                    7301 bp    DNA     circular     14-SEP-2012
DEFINITION  LMBP 7765.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7765
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 14-SEP-2012
            
COMMENT     VNTDATE|628880324|
COMMENT     VNTNAME|BH20|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "BH20" 0 7301 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 350 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "HindIII" "KpnI" "BglI" "HincII" "PvuII" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 3475 3474 1 7301 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 174 0 358 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 2118 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 1 4120 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HincII" "gtyrac" 4 0 6 766 0 999 0 1113 0 1561 
COMMENT            0 1630 0 3562 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 1262 0 3656 0 3749 0 
COMMENT            4748 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 826 0 2264 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "T7 terminator" 43 0 1 26 73 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(26..73)") 
COMMENT        #7=(CFSignal (CObList) "His-tag" 22 0 1 140 157 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(140..157)") 
COMMENT        #8=(CFSignal (CObList) "His-tag" 22 0 1 2206 2223 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2206..2223)") 
COMMENT        #9=(CFSignal (CObList) "T7 promoter with lac operator" 30 0 1 2273 2314 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "complement(2271..2314)") 
COMMENT        #10=(CFSignal (CObList) "lacI promoter, incl. RBS" 30 0 0 2627 2704 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2627..2704") 
COMMENT        #11=(CFSignal (CObList) "rop" 4 0 0 4596 4787 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4596..4787") 
COMMENT        #12=(CFSignal (CObList) "pMB1 ori" 33 0 1 5033 5435 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5033..5435)") 
COMMENT        #13=(CFSignal (CObList) "lacI" 4 0 0 2705 3787 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2705..3787") 
COMMENT        #14=(CFSignal (CObList) "Tn903 neomycin resistance" 4 0 0 5927 6742 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5927..6742") 
COMMENT        #15=(CFSignal (CObList) "f1 ori" 33 0 1 6835 7290 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(6835..7290)") 
COMMENT        #16=(CFSignal (CObList) "A" 4 0 1 822 2003 0 (CStringList) (CStringList) 
COMMENT             1 1 1 1 "complement(822..2003)") 
COMMENT        #17=(CFSignal (CObList) "COAT" 4 0 1 406 798 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(406..798)") 
COMMENT        #18=(CFSignal (CObList) "RT-PCR positive control DNA" 21 0 1 179 356 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(179..356)") 
COMMENT        #19=(CFSignal (CObList) "5' UTR of T7 gene 10, incl. RBS" 32 0 1 2227 
COMMENT             2271 0 (CStringList) (CStringList) 1 1 1 1 "complement(2227..2270)") 
COMMENT        #20=(CFSignal (CObList) "thrombin recognition site" 22 0 1 2179 2196 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2179..2196)") 
COMMENT        #21=(CFSignal (CObList) "S-tag" 22 0 1 2128 2172 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2128..2172)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #22=(CFSignal (CObList) "LMBP 7765" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "Last modified: 14-SEP-2012" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #24=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #25=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "BH20" 2) 5 "" 0 4) 
COMMENT              #26=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'BH20'" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 7765." 
COMMENT                          1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 06/14/12 04:58" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 09/14/12 01:55" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7301 bp" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #35=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 14-SEP-2012" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #38=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #39=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #40=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #41=(CLinePar (CParagraph 0 (15 #22# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 7765") 
COMMENT                     #42=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #43=(CLinePar (CParagraph 0 (15 #23# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 14-SEP-2012"))) 
COMMENT                   "Annotations") 
COMMENT              #44=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #46=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 406 (3 #17# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 406   End: 798  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "MS2 sequence encoding the coat protein" 
COMMENT                                        1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(406..798)" 1))) "COAT") 
COMMENT                            #51=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 822 (3 #16# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 822   End: 2003 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "MS2 sequence encoding the assembly protein" 
COMMENT                                        1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(822..2003)" 1))) "A") 
COMMENT                            #56=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2705 (3 #13# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #57=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2705  End: 3787" 1) 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2705..3787" 1))) "lacI") 
COMMENT                            #60=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4596 (3 #11# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4596  End: 4787" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4596..4787" 1))) "rop") 
COMMENT                            #64=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5927 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5927  End: 6742" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5927..6742" 1))) 
COMMENT                                 "Tn903 neomycin resistance"))) "CDS (5 total)") 
COMMENT                     #68=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #69=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 179 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 179   End: 356  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(179..356)" 1))) 
COMMENT                                 "RT-PCR positive control DNA"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #73=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 140 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 140   End: 157  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(140..157)" 1))) "His-tag") 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2128 (3 #21# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2128  End: 2172 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2128..2172)" 1))) "S-tag") 
COMMENT                            #82=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2179 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2179  End: 2196 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2179..2196)" 1))) 
COMMENT                                 "thrombin recognition site") 
COMMENT                            #86=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2206 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2206  End: 2223 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2206..2223)" 1))) 
COMMENT                                 "His-tag"))) "Misc. Marker (4 total)") 
COMMENT                     #90=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2273 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2273  End: 2314 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "T7 promoter at position 2298 to 2314; lac operator at position 2273 to 2293" 
COMMENT                                        1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2271..2314)" 1))) 
COMMENT                                 "T7 promoter with lac operator") 
COMMENT                            #96=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2627 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2627  End: 2704" 1) 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2627..2704" 1))) 
COMMENT                                 "lacI promoter, incl. RBS"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
COMMENT                     #100=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 32 (7 32 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #101=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2227 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #102=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2227  End: 2271 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #103=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #104=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(2227..2270)" 1))) 
COMMENT                                   "5' UTR of T7 gene 10, incl. RBS"))) 
COMMENT                           "RBS (1 total)") 
COMMENT                     #105=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #106=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5033 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #107=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5033  End: 5435 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(5033..5435)" 1))) 
COMMENT                                   "pMB1 ori") 
COMMENT                             #110=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 6835 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 6835  End: 7290 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(6835..7290)" 1))) 
COMMENT                                   "f1 ori"))) "Replication Origin (2 total)") 
COMMENT                     #114=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #115=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 26 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 26    End: 73   (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #117=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #118=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(26..73)" 1))) 
COMMENT                                   "T7 terminator"))) "Terminator (1 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #119=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #120=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #121=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #122=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2118 " 1))))) "KpnI: 1 site") 
COMMENT                            5 1 "GGTACC" "CCATGG") 
COMMENT                      #123=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #124=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #125=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 4120 " 1))))) "BglI: 1 site") 
COMMENT                            7 4 "GCCNNNNNGGC" "CGGNNNNNCCG") 
COMMENT                      #126=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #127=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #128=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 174 " 1) 
COMMENT                                      #129=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 358 " 1))))) 
COMMENT                             "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #130=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 2 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #131=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #132=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 826 " 1) 
COMMENT                                      #133=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2264 " 1))))) "XbaI: 2 sites") 
COMMENT                            1 5 "TCTAGA" "AGATCT") 
COMMENT                      #134=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 4 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #135=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #136=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1262 " 1) 
COMMENT                                      #137=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3656 " 1) 
COMMENT                                      #138=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 3749 " 1) 
COMMENT                                      #139=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 4748 " 1))))) "PvuII: 4 sites") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
COMMENT                      #140=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 6 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #141=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #142=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 766 " 1) 
COMMENT                                      #143=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 999 " 1) 
COMMENT                                      #144=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                            " N3: 1113 " 1) 
COMMENT                                      #145=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 4) 1 2 2 0 191) 
COMMENT                                            " N4: 1561 " 1) 
COMMENT                                      #146=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 5) 1 2 2 0 191) 
COMMENT                                            " N5: 1630 " 1) 
COMMENT                                      #147=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 6) 1 2 2 0 191) 
COMMENT                                            " N6: 3562 " 1))))) 
COMMENT                             "HincII: 6 sites") 3 3 "GTYRAC" "CARYTG"))) 
COMMENT                    "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #148=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #149=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #150=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #151=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #152=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #153=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #154=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) 
COMMENT          #155=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #156=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #157=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #158=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #159=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #160=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #161=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #162=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #163=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #164=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #165=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #166=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) #167=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #168=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #169=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #170=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #171=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #172=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #173=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #174=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #175=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #176=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #177=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #178=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #179=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #180=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #181=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #182=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #183=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #184=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #185=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #186=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #187=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #188=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #189=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #190=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #191=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #192=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #193=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #194=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #195=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #196=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #197=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #198=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #199=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #200=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #201=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #202=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #203=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #204=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #205=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #206=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #207=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -3261) 1 1 0 1 1 
COMMENT        (mapper: 22.9002 -19.9857 40.3368 40.3368 0.01 10 -1 7301 7301 1 0 0) 
COMMENT        #208=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -461 100) 
COMMENT              (CObjectList 
COMMENT               #209=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -722 100) 
COMMENT                     (CObjectList 
COMMENT                      #210=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #154# 28565484 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27504 
COMMENT                             6.27304 1 0.0214037) 0.0859223) 
COMMENT                      #211=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #151# 1 100) 
COMMENT                            (LOGPEN 0 0 3381657) 1 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "BH20" "@N" 1 0 0.871165 0 -10 
COMMENT                            3.44598 1.56185 Nil) 
COMMENT                      #212=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #150# -956 100) 
COMMENT                            (LOGPEN 0 0 3381657) 1 
COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "7301 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 4.75992 1.80976 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #213=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1132 100) 
COMMENT                     (CObjectList 
COMMENT                      #214=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -889 100) 
COMMENT                            (CObjectList 
COMMENT                             #215=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #152# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    2.16009 2.32506 1 0.082322) 0.8 1.8 0) 
COMMENT                             #216=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #192# 27640644 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "rop" "@N" 1 
COMMENT                                   -11.7563 -10.1991 0 -10 1.4131 1.16519 #215#) 
COMMENT                             #217=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #152# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    3.01931 3.94985 1 0.082322) 0.8 1.8 0) 
COMMENT                             #218=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #192# 2081903016 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lacI" "@N" 1 
COMMENT                                   4.59097 -12.5602 0 -10 1.4131 1.16519 #217#) 
COMMENT                             #219=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #152# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.480306 1.18143 1 0.082322) 0.8 1.8 0) 
COMMENT                             #220=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #192# 1195852611 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tn903 neomycin resistance" "@N" 1 -15.6144 
COMMENT                                   9.38101 0 -10 11.0817 1.16519 #219#) 
COMMENT                             #221=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #16# 0) 1 2 0 0 #152# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.55216 5.56776 1 0.082322) 0.8 1.8 1) 
COMMENT                             #222=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #192# 27415268 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "A" "@N" 1 
COMMENT                                   14.0616 5.51457 0 -10 0.768529 1.16519 #221#) 
COMMENT                             #223=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #152# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    5.58753 5.9252 1 0.082322) 0.8 1.8 1) 
COMMENT                             #224=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #192# 14746136 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "COAT" "@N" 1 
COMMENT                                   7.68915 12.349 0 -10 2.50392 1.16519 #223#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #225=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -2014 100) 
COMMENT                            (CObjectList 
COMMENT                             #226=(CLine 
COMMENT                                   (CWidget 0 (3 #18# 0) 1 2 0 0 #155# 
COMMENT                                    1886220099 100) (LOGPEN 0 6 16711680) 8 
COMMENT                                   0.835356 5.9673 6.12024) 
COMMENT                             #227=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #193# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "RT-PCR positive control DNA" "@N" 1 8.81748 
COMMENT                                   14.9908 0 -10 11.4784 1.16519 #226#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #228=(CGroupWidget 
COMMENT                            (CWidget 22 (7 22 0) 1 2 0 0 Nil -397 100) 
COMMENT                            (CObjectList 
COMMENT                             #229=(CScratch 
COMMENT                                   (CWidget 0 (3 #7# 0) 1 2 0 0 #191# 495 100) 
COMMENT                                   (LOGPEN 0 4 10040064) 6 1 6.14688 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #230=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #192# 28412716 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "His-tag" "@N" 1 
COMMENT                                   3.30719 17.6573 0 -10 2.90058 1.16519 #229#) 
COMMENT                             #231=(CScratch 
COMMENT                                   (CWidget 0 (3 #8# 0) 1 2 0 0 #191# 1085 100) 
COMMENT                                   (LOGPEN 0 4 10040064) 6 1 4.37173 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #232=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #192# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "His-tag" "@N" 1 
COMMENT                                   16.4382 -4.76587 0 -10 2.90058 1.16519 #231#) 
COMMENT                             #233=(CScratch 
COMMENT                                   (CWidget 0 (3 #20# 0) 1 2 0 0 #191# 1081 100) 
COMMENT                                   (LOGPEN 0 4 10040064) 6 1 4.39492 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #234=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #192# 1886220099 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "thrombin recognition site" "@N" 1 19.454 
COMMENT                                   -2.94917 0 -10 9.74296 1.16519 #233#) 
COMMENT                             #235=(CScratch 
COMMENT                                   (CWidget 0 (3 #21# 0) 1 2 0 0 #191# 765 100) 
COMMENT                                   (LOGPEN 0 4 10040064) 6 1 4.42672 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #236=(CLabel (CWidget 0 (0 0) 1 2 0 0 #192# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "S-tag" "@N" 1 
COMMENT                                   15.6207 -1.62829 0 -10 2.256 1.16519 #235#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #237=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -797 100) 
COMMENT                            (CObjectList 
COMMENT                             #238=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #171# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 0.835356 4.32103 4.4083) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #239=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #192# 14746160 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "T7 promoter with lac operator" "@N" 1 20.108 
COMMENT                                   -9.13608 0 -10 11.8998 1.16519 #238#) 
COMMENT                             #240=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #194# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    3.94985 4.01687 1 0.082322) 0.8 1.8 0) 
COMMENT                             #241=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #192# 1094796097 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "lacI promoter, incl. RBS" "@N" 1 14.2538 
COMMENT                                   -10.4956 0 -10 9.24714 1.16519 #240#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #242=(CGroupWidget 
COMMENT                            (CWidget 32 (7 32 0) 1 2 0 0 Nil -722 100) 
COMMENT                            (CObjectList 
COMMENT                             #243=(CScratch 
COMMENT                                   (CWidget 0 (3 #19# 0) 1 2 0 0 #169# 765 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 1 4.36056 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #244=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #193# 2424836 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "5' UTR of T7 gene 10, incl. RBS" "@N" 1 
COMMENT                                   20.885 -6.34161 0 -10 12.47 1.16519 #243#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #245=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -1100 100) 
COMMENT                            (CObjectList 
COMMENT                             #246=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #164# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    1.60331 1.94958 1 0.082322) 0.8 1.8 1) 
COMMENT                             #247=(CLabel (CWidget 0 (0 0) 1 2 0 0 #192# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -14.9083 -3.15543 0 -10 3.56994 1.16519 
COMMENT                                   #246#) 
COMMENT                             #248=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #164# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    0.00945145 0.401257 1 0.082322) 0.8 1.8 1) 
COMMENT                             #249=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #192# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 ori" "@N" 1 
COMMENT                                   -3.87079 14.1796 0 -10 2.15684 1.16519 #248#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #250=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -889 100) 
COMMENT                            (CObjectList 
COMMENT                             #251=(CScratch 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #155# 765 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 1 6.2517 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #252=(CLabel (CWidget 0 (0 0) 1 2 0 0 #202# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 terminator" 
COMMENT                                   "@N" 1 -2.49276 15.8297 0 -10 5.30533 1.16519 
COMMENT                                   #251#)) (CObjectList))) (CObjectList)) 
COMMENT               #253=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil 601 100) 
COMMENT                     (CObjectList 
COMMENT                      #254=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil 1701672291 100) 
COMMENT                            (CObjectList 
COMMENT                             #255=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #207# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 4.45421 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #256=(CLabel (CWidget 0 (0 0) 1 2 0 0 #204# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Kpn}I (2118)" "@N (@S)" 5 16.351 
COMMENT                                   -0.133875 0 -10 4.2641 0.99165 #255#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #257=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil 1953067617 100) 
COMMENT                            (CObjectList 
COMMENT                             #258=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #207# 491 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 2.73405 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #259=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #204# 1094918202 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Bgl}I (4120)" "@N (@S)" 5 -6.8202 
COMMENT                                   -12.3629 0 -10 4.01618 0.99165 #258#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #260=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #0# 0) 1 2 0 0 Nil -468 100) 
COMMENT                            (CObjectList 
COMMENT                             #261=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #206# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 6.12454 1.9 0.082322 1) 
COMMENT                             #262=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 28412460 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (174)" "@N (@S)" 5 4.75007 
COMMENT                                   16.2621 0 -10 4.56159 0.99165 #261#) 
COMMENT                             #263=(CScratch 
COMMENT                                   (CWidget 2 (1 #0# 2) 1 2 0 0 #206# 765 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 5.96644 1.9 0.082322 1) 
COMMENT                             #264=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 131074 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hin}dIII (358)" "@N (@S)" 5 6.3563 
COMMENT                                   13.6699 0 -10 4.56159 0.99165 #263#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #265=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #5# 0) 1 2 0 0 Nil 1953067617 100) 
COMMENT                            (CObjectList 
COMMENT                             #266=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #206# 1013 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 5.56433 1.9 0.082322 1) 
COMMENT                             #267=(CLabel (CWidget 0 (0 0) 1 2 0 0 #205# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Xba}I (826)" "@N (@S)" 5 10.1696 
COMMENT                                   9.95518 0 -10 3.74348 0.99165 #266#) 
COMMENT                             #268=(CScratch 
COMMENT                                   (CWidget 2 (1 #5# 2) 1 2 0 0 #206# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 4.32876 1.9 0.082322 1) 
COMMENT                             #269=(CLabel (CWidget 0 (0 0) 1 2 0 0 #205# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Xba}I (2264)" "@N (@S)" 5 16.6304 
COMMENT                                   -7.66249 0 -10 4.16493 0.99165 #268#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #270=(CGroupWidget 
COMMENT                            (CWidget 4 (10 #4# 0) 1 2 0 0 Nil 1953067617 100) 
COMMENT                            (CObjectList 
COMMENT                             #271=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #206# 761 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 5.18971 1.9 0.082322 1) 
COMMENT                             #272=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 27598208 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Pvu}II (1262)" "@N (@S)" 5 14.0085 
COMMENT                                   6.78586 0 -10 4.31368 0.99165 #271#) 
COMMENT                             #273=(CScratch 
COMMENT                                   (CWidget 2 (1 #4# 2) 1 2 0 0 #206# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.13273 1.9 0.082322 1) 
COMMENT                             #274=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 1668238368 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Pvu}II (3656)" "@N (@S)" 5 -2.48702 
COMMENT                                   -15.4261 0 -10 4.31368 0.99165 #273#) 
COMMENT                             #275=(CScratch 
COMMENT                                   (CWidget 3 (1 #4# 3) 1 2 0 0 #206# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.05282 1.9 0.082322 1) 
COMMENT                             #276=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 2082602612 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Pvu}II (3749)" "@N (@S)" 5 -3.40125 
COMMENT                                   -13.8573 0 -10 4.31368 0.99165 #275#) 
COMMENT                             #277=(CScratch 
COMMENT                                   (CWidget 4 (1 #4# 4) 1 2 0 0 #206# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 2.19445 1.9 0.082322 1) 
COMMENT                             #278=(CLabel (CWidget 0 (0 0) 1 2 0 0 #205# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Pvu}II (4748)" "@N (@S)" 5 -13.8797 
COMMENT                                   -8.4568 0 -10 4.31368 0.99165 #277#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #279=(CGroupWidget 
COMMENT                            (CWidget 6 (10 #3# 0) 1 2 0 0 Nil 1953067617 100) 
COMMENT                            (CObjectList 
COMMENT                             #280=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #206# 765 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 5.61588 1.9 0.082322 1) 
COMMENT                             #281=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 27591612 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (766)" "@N (@S)" 5 9.64589 
COMMENT                                   11.1025 0 -10 4.23931 0.99165 #280#) 
COMMENT                             #282=(CScratch 
COMMENT                                   (CWidget 2 (1 #3# 2) 1 2 0 0 #206# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 5.41568 1.9 0.082322 1) 
COMMENT                             #283=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (999)" "@N (@S)" 5 11.9247 
COMMENT                                   8.78305 0 -10 4.23931 0.99165 #282#) 
COMMENT                             #284=(CScratch 
COMMENT                                   (CWidget 3 (1 #3# 3) 1 2 0 0 #206# 765 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 5.31773 1.9 0.082322 1) 
COMMENT                             #285=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 1096045635 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (1113)" "@N (@S)" 5 13.0454 
COMMENT                                   7.75966 0 -10 4.63597 0.99165 #284#) 
COMMENT                             #286=(CScratch 
COMMENT                                   (CWidget 4 (1 #3# 4) 1 2 0 0 #206# 1210 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 4.9328 1.9 0.082322 1) 
COMMENT                             #287=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #205# 1094796097 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (1561)" "@N (@S)" 5 15.6909 
COMMENT                                   3.62349 0 -10 4.63597 0.99165 #286#) 
COMMENT                             #288=(CScratch 
COMMENT                                   (CWidget 5 (1 #3# 5) 1 2 0 0 #206# 748 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 4.87351 1.9 0.082322 1) 
COMMENT                             #289=(CLabel (CWidget 0 (0 0) 1 2 0 0 #205# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (1630)" "@N (@S)" 5 16.1357 
COMMENT                                   2.35219 0 -10 4.63597 0.99165 #288#) 
COMMENT                             #290=(CScratch 
COMMENT                                   (CWidget 6 (1 #3# 6) 1 2 0 0 #206# 0 100) 
COMMENT                                   (LOGPEN 0 0 39321) 1 1 3.21349 1.9 0.082322 1) 
COMMENT                             #291=(CLabel (CWidget 0 (0 0) 1 2 0 0 #205# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381657) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32896 
COMMENT                                   "{\\i Hinc}II (3562)" "@N (@S)" 5 3.53255 
COMMENT                                   -15.4678 0 -10 4.63597 0.99165 #290#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #292=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 1953067617 100) 
COMMENT                     (CObjectList 
COMMENT                      #293=(CLabel 
COMMENT                            (CWidget 0 (15 #22# 0) 1 2 0 0 #177# 2082602612 100) 
COMMENT                            (LOGPEN 12 0 1277193313) 1818583649 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 7765" 
COMMENT                            "LMBP 7765" 65535 0 -2.87579 0 -10 6.84239 1.56185 
COMMENT                            Nil) 
COMMENT                      #294=(CLabel 
COMMENT                            (CWidget 0 (15 #23# 0) 1 2 0 0 #177# 14746016 100) 
COMMENT                            (LOGPEN 1 -365 1400137063) 0 
COMMENT                            (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 14-SEP-2012" 
COMMENT                            "Last modified: 14-SEP-2012" 65535 0 -5.03263 0 -10 
COMMENT                            10.8338 1.06602 Nil)) (CObjectList)) 
COMMENT               #295=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1953067617 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 1196705607 
COMMENT       (CStringList) 1094795585 1195852099 (CObList)))
FEATURES             Location/Qualifiers
     terminator      complement(26..73)
                     /vntifkey="43"
                     /label=T7\terminator
     misc_marker     complement(140..157)
                     /vntifkey="22"
                     /label=His-tag
     misc_marker     complement(2206..2223)
                     /vntifkey="22"
                     /label=His-tag
     promoter        complement(2273..2314)
                     /vntifkey="30"
                     /label=T7\promoter\with\lac\operator
                     /note="T7 promoter at position 2298 to 2314; lac operator at position 2273 to 2293"
     promoter        2627..2704
                     /vntifkey="30"
                     /label=lacI\promoter,\incl.\RBS
     CDS             4596..4787
                     /vntifkey="4"
                     /label=rop
     rep_origin      complement(5033..5435)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             2705..3787
                     /vntifkey="4"
                     /label=lacI
     CDS             5927..6742
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     rep_origin      complement(6835..7290)
                     /vntifkey="33"
                     /label=f1\ori
     CDS             complement(822..2003)
                     /vntifkey="4"
                     /label=A
                     /note="MS2 sequence encoding the assembly protein"
     CDS             complement(406..798)
                     /vntifkey="4"
                     /label=COAT
                     /note="MS2 sequence encoding the coat protein"
     misc_feature    complement(179..356)
                     /vntifkey="21"
                     /label=RT-PCR\positive\control\DNA
     RBS             complement(2227..2271)
                     /vntifkey="32"
                     /label=5'\UTR\of\T7\gene\10,\incl.\RBS
     misc_marker     complement(2179..2196)
                     /vntifkey="22"
                     /label=thrombin\recognition\site
     misc_marker     complement(2128..2172)
                     /vntifkey="22"
                     /label=S-tag
BASE COUNT     1750 a      1873 c      1941 g      1737 t 
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttc 
      181 tgcaaaaaca tcttcaagtc tctgagctgt tgcaacccca agcctgagga gaggcatttg 
      241 ctaagatcta ctagaaggca gggatgatgt tctggagagc cctctattgc acttgccccg 
      301 tagctggatg tcctcacctg catacgcgtt cgacctcggt tagaagactc atctccaagc 
      361 tttcttcgac atgggtaatc ctcatgtttg aatggccggc gtctattagt agatgccgga 
      421 gtttgctgcg attgctgagg gaatcgggtt tccatctttt aggagacctt gcattgcctt 
      481 aacaataagc tcgcagtcgg aattcgtggc gaaaattgga atggttagtt ccatatttaa 
      541 gtacgaacgc catgcggcta caggaagctc tacaccacca acagtctggg ttgccacttt 
      601 aggcacctcg actttgatgg tgtatttgcg attctgcgca gagctctgac gaacgctaca 
      661 ggttactttg taagcctgtg aacgcgagtt agagctgatc cattcagcga ccccgttagc 
      721 gaagttgctt ggggcgacag tcacgtcgcc agttccgcca ttgtcgacga gaacgaactg 
      781 agtaaagtta gaagccatgc ttcaaactcc ggttgagggc tctatctaga gagccgttgc 
      841 ctgattaatg ctaacgcatc taaggtatgg accatcgaga aaggagactt tacgtacgcg 
      901 ccagttgttg gccatacgga ttgtacccct cgatgcatgg ctgagatttg ggccttagca 
      961 gtgccctgtc tctccacagt ccacccgtag ggagcgtcaa cgcttatgat ggactcaccc 
     1021 gttattacgt cagtaactgt tcctgacatg taggagcatc ccacgggggc cgtaaggccc 
     1081 tcgagcatgt tacctacagg taggagccag tcgacaacga atgagaaagg caccttttcc 
     1141 cacactatac ctagtgggtt caagatacct agagacgaca accatgccaa acgtgcatcg 
     1201 tttatgtaaa accatatcac gatacgtcgc gatatgttgc acgttgtctg gaagtttgca 
     1261 gctggatacg acagacggcc atctaacttg atgttagtac cgacctgacg tacggctctc 
     1321 ataggaagaa actcttgaag gtgaaccttc gtaagcatct catatgcacc ctggatatca 
     1381 ctcattagtg gtaaccaacc gaactgcaac tccaaccacc tgccggccac gtgttttgat 
     1441 cgaaactttc gatcttcgtt tagggcaagg tagcggagcg cctggcgcca attaccgcga 
     1501 cgagcggcag tgtacgcctt cacgagcgca atggtttgcg tcgcgagttg tgaggctgtc 
     1561 gacctggcct ctgctaaagc aacaccaagg ttaaaattac cctgggtgac cttttgcagg 
     1621 acttcggtcg acgcccggtt cgcaacgttc tgcggcactt cgatgtaagt caagttttgg 
     1681 cttacaggga agaggctgta gcaggagcgt gcgtcgaggg agaagccgaa accggctttc 
     1741 tcctcgtacg ggcgacccca cgatgaccca cttcgcttgt aggcaccttg atctatcgat 
     1801 gtgacactta acgccccccg tgaatacgga gaggggtagt gccactgttt cgttttggcc 
     1861 ccagtcgagt taaaacgacc gggagtccag ttcgaacgat attttaaaga gaatgagtta 
     1921 tcttcagtct caccgtccgc gtaaacgcga acggagggga cgaaggtctc gttctcccta 
     1981 tcaagggtac taaaagctcg cacaggtcaa acctcctagg aatggaattc cggctaccta 
     2041 cagcgatagc catggtagcg tctcgctaaa gacattaaaa atggcattag ctcgacagga 
     2101 agttgagcag gaggtaccca gatctgggct gtccatgtgc tggcgttcga atttagcagc 
     2161 agcggtttct ttcataccag aaccgcgtgg caccagacca gaagaatgat gatgatgatg 
     2221 gtgcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta 
     2281 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atcgatctcg 
     2341 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc 
     2401 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc 
     2461 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc 
     2521 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc 
     2581 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg 
     2641 gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt 
     2701 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac 
     2761 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga 
     2821 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa 
     2881 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat 
     2941 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt 
     3001 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt 
     3061 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc 
     3121 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat 
     3181 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca 
     3241 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc 
     3301 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga 
     3361 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc 
     3421 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga 
     3481 gtccgggctg cgcgttggtg cggacatctc ggtagtggga tacgacgata ccgaagacag 
     3541 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 
     3601 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt 
     3661 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc 
     3721 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 
     3781 gcagtgagcg caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac 
     3841 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta 
     3901 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag 
     3961 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt 
     4021 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca 
     4081 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggcccca cgggtgcgca 
     4141 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc 
     4201 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga 
     4261 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga 
     4321 agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct 
     4381 gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct 
     4441 ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat 
     4501 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc 
     4561 ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc 
     4621 ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc 
     4681 tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt 
     4741 accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 
     4801 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 
     4861 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 
     4921 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 
     4981 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc 
     5041 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     5101 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 
     5161 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 
     5221 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 
     5281 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 
     5341 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 
     5401 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 
     5461 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 
     5521 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 
     5581 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 
     5641 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 
     5701 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 
     5761 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 
     5821 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 
     5881 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca 
     5941 acgggaaacg tcttgctcta ggccgcgatt aaattccaac atggatgctg atttatatgg 
     6001 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg 
     6061 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt 
     6121 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa 
     6181 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac 
     6241 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc 
     6301 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg 
     6361 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga 
     6421 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact 
     6481 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat 
     6541 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg 
     6601 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa 
     6661 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt 
     6721 gatgctcgat gagtttttct aagaattaat tcatgagcgg atacatattt gaatgtattt 
     6781 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg 
     6841 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 
     6901 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 
     6961 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 
     7021 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 
     7081 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 
     7141 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 
     7201 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 
     7261 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc a 
//
