ID BH20 standard; circular DNA; ; 7301 BP. DE LMBP 7765. CC Accession number: LMBP 7765 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 14-SEP-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator complement(26..73) FT /vntifkey="43" FT /label=T7\terminator FT misc_marker complement(140..157) FT /vntifkey="22" FT /label=His-tag FT misc_marker complement(2206..2223) FT /vntifkey="22" FT /label=His-tag FT promoter complement(2273..2314) FT /vntifkey="30" FT /label=T7\promoter\with\lac\operator FT /note="T7 promoter at position 2298 to 2314; lac operator at position 2273 to 2293" FT promoter 2627..2704 FT /vntifkey="30" FT /label=lacI\promoter,\incl.\RBS FT CDS 4596..4787 FT /vntifkey="4" FT /label=rop FT rep_origin complement(5033..5435) FT /vntifkey="33" FT /label=pMB1\ori FT CDS 2705..3787 FT /vntifkey="4" FT /label=lacI FT CDS 5927..6742 FT /vntifkey="4" FT /label=Tn903\neomycin\resistance FT rep_origin complement(6835..7290) FT /vntifkey="33" FT /label=f1\ori FT CDS complement(822..2003) FT /vntifkey="4" FT /label=A FT /note="MS2 sequence encoding the assembly protein" FT CDS complement(406..798) FT /vntifkey="4" FT /label=COAT FT /note="MS2 sequence encoding the coat protein" FT misc_feature complement(179..356) FT /vntifkey="21" FT /label=RT-PCR\positive\control\DNA FT RBS complement(2227..2271) FT /vntifkey="32" FT /label=5'\UTR\of\T7\gene\10,\incl.\RBS FT misc_marker complement(2179..2196) FT /vntifkey="22" FT /label=thrombin\recognition\site FT misc_marker complement(2128..2172) FT /vntifkey="22" FT /label=S-tag SQ Sequence 7301 BP; 1749 A; 1873 C; 1942 G; 1737 t; atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttc 180 tgcaaaaaca tcttcaagtc tctgagctgt tgcaacccca agcctgagga gaggcatttg 240 ctaagatcta ctagaaggca gggatgatgt tctggagagc cctctattgc acttgccccg 300 tagctggatg tcctcacctg catacgcgtt cgacctcggt tagaagactc atctccaagc 360 tttcttcgac atgggtaatc ctcatgtttg aatggccggc gtctattagt agatgccgga 420 gtttgctgcg attgctgagg gaatcgggtt tccatctttt aggagacctt gcattgcctt 480 aacaataagc tcgcagtcgg aattcgtggc gaaaattgga atggttagtt ccatatttaa 540 gtacgaacgc catgcggcta caggaagctc tacaccacca acagtctggg ttgccacttt 600 aggcacctcg actttgatgg tgtatttgcg attctgcgca gagctctgac gaacgctaca 660 ggttactttg taagcctgtg aacgcgagtt agagctgatc cattcagcga ccccgttagc 720 gaagttgctt ggggcgacag tcacgtcgcc agttccgcca ttgtcgacga gaacgaactg 780 agtaaagtta gaagccatgc ttcaaactcc ggttgagggc tctatctaga gagccgttgc 840 ctgattaatg ctaacgcatc taaggtatgg accatcgaga aaggagactt tacgtacgcg 900 ccagttgttg gccatacgga ttgtacccct cgatgcatgg ctgagatttg ggccttagca 960 gtgccctgtc tctccacagt ccacccgtag ggagcgtcaa cgcttatgat ggactcaccc 1020 gttattacgt cagtaactgt tcctgacatg taggagcatc ccacgggggc cgtaaggccc 1080 tcgagcatgt tacctacagg taggagccag tcgacaacga atgagaaagg caccttttcc 1140 cacactatac ctagtgggtt caagatacct agagacgaca accatgccaa acgtgcatcg 1200 tttatgtaaa accatatcac gatacgtcgc gatatgttgc acgttgtctg gaagtttgca 1260 gctggatacg acagacggcc atctaacttg atgttagtac cgacctgacg tacggctctc 1320 ataggaagaa actcttgaag gtgaaccttc gtaagcatct catatgcacc ctggatatca 1380 ctcattagtg gtaaccaacc gaactgcaac tccaaccacc tgccggccac gtgttttgat 1440 cgaaactttc gatcttcgtt tagggcaagg tagcggagcg cctggcgcca attaccgcga 1500 cgagcggcag tgtacgcctt cacgagcgca atggtttgcg tcgcgagttg tgaggctgtc 1560 gacctggcct ctgctaaagc aacaccaagg ttaaaattac cctgggtgac cttttgcagg 1620 acttcggtcg acgcccggtt cgcaacgttc tgcggcactt cgatgtaagt caagttttgg 1680 cttacaggga agaggctgta gcaggagcgt gcgtcgaggg agaagccgaa accggctttc 1740 tcctcgtacg ggcgacccca cgatgaccca cttcgcttgt aggcaccttg atctatcgat 1800 gtgacactta acgccccccg tgaatacgga gaggggtagt gccactgttt cgttttggcc 1860 ccagtcgagt taaaacgacc gggagtccag ttcgaacgat attttaaaga gaatgagtta 1920 tcttcagtct caccgtccgc gtaaacgcga acggagggga cgaaggtctc gttctcccta 1980 tcaagggtac taaaagctcg cacaggtcaa acctcctagg aatggaattc cggctaccta 2040 cagcgatagc catggtagcg tctcgctaaa gacattaaaa atggcattag ctcgacagga 2100 agttgagcag gaggtaccca gatctgggct gtccatgtgc tggcgttcga atttagcagc 2160 agcggtttct ttcataccag aaccgcgtgg caccagacca gaagaatgat gatgatgatg 2220 gtgcatatgt atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta 2280 tccgctcaca attcccctat agtgagtcgt attaatttcg cgggatcgag atcgatctcg 2340 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc 2400 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc 2460 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc 2520 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc 2580 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg 2640 gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt 2700 gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac 2760 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga 2820 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa 2880 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat 2940 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt 3000 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt 3060 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc 3120 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat 3180 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca 3240 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc 3300 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga 3360 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc 3420 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga 3480 gtccgggctg cgcgttggtg cggacatctc ggtagtggga tacgacgata ccgaagacag 3540 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 3600 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt 3660 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc 3720 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 3780 gcagtgagcg caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac 3840 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta 3900 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag 3960 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt 4020 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca 4080 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggcccca cgggtgcgca 4140 tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc 4200 agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga 4260 cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga 4320 agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct 4380 gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct 4440 ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat 4500 gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc 4560 ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc 4620 ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc 4680 tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt 4740 accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 4800 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 4860 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 4920 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 4980 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc 5040 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 5100 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 5160 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 5220 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 5280 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 5340 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 5400 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 5460 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 5520 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 5580 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 5640 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 5700 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 5760 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 5820 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 5880 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca 5940 acgggaaacg tcttgctcta ggccgcgatt aaattccaac atggatgctg atttatatgg 6000 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg 6060 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt 6120 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa 6180 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac 6240 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc 6300 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg 6360 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga 6420 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact 6480 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat 6540 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg 6600 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa 6660 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt 6720 gatgctcgat gagtttttct aagaattaat tcatgagcgg atacatattt gaatgtattt 6780 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg 6840 taaacgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 6900 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 6960 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 7020 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 7080 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 7140 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 7200 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 7260 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc a 7301 //