LOCUS       AU2      7117 bp    DNA     circular UNA 1-DEC-2020
DEFINITION  LMBP 7766.
COMMENT     Accession number: LMBP 7766
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 1-DEC-2020
            NGS data implemented
FEATURES             Location/Qualifiers
     terminator      complement(26..73)
                     /label="T7 terminator"
     misc_feature    complement(140..157)
                     /label="His-tag"
     CDS             complement(222..614)
                     /label="COAT"
                     /note="MS2 sequence encoding the coat protein"
     CDS             complement(638..1819)
                     /label="A"
                     /note="MS2 sequence encoding the assembly protein"
     misc_feature    complement(1944..1988)
                     /label="S-tag"
     misc_feature    complement(1995..2012)
                     /label="thrombin recognition site"
     misc_feature    complement(2022..2039)
                     /label="His-tag"
     RBS             complement(2043..2087)
                     /label="5' UTR of T7 gene 10, incl. RBS"
     promoter        complement(2089..2130)
                     /label="T7 promoter with lac operator"
     promoter        2443..2520
                     /label="lacI promoter, incl. RBS"
     CDS             2521..3603
                     /label="lacI"
     CDS             4412..4603
                     /label="rop"
     rep_origin      complement(4849..5251)
                     /label="pMB1 ori"
     CDS             5743..6558
                     /label="Tn903 neomycin resistance"
     rep_origin      complement(6651..7106)
                     /label="f1 ori"
ORIGIN
        1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
       61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
      121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTTC
      181 TTCGACATGG GTAATCCTCA TGTTTGAATG GCCGGCGTCT ATTAGTAGAT GCCGGAGTTT
      241 GCTGCGATTG CTGAGGGAAT CGGGTTTCCA TCTTTTAGGA GACCTTGCAT TGCCTTAACA
      301 ATAAGCTCGC AGTCGGAATT CGTGGCGAAA ATTGGAATGG TTAGTTCCAT ATTTAAGTAC
      361 GAACGCCATG CGGCTACAGG AAGCTCTACA CCACCAACAG TCTGGGTTGC CACTTTAGGC
      421 ACCTCGACTT TGATGGTGTA TTTGCGATTC TGCGCAGAGC TCTGACGAAC GCTACAGGTT
      481 ACTTTGTAAG CCTGTGAACG CGAGTTAGAG CTGATCCATT CAGCGACCCC GTTAGCGAAG
      541 TTGCTTGGGG CGACAGTCAC GTCGCCAGTT CCGCCATTGT CGACGAGAAC GAACTGAGTA
      601 AAGTTAGAAG CCATGCTTCA AACTCCGGTT GAGGGCTCTA TCTAGAGAGC CGTTGCCTGA
      661 TTAATGCTAA CGCATCTAAG GTATGGACCA TCGAGAAAGG AGACTTTACG TACGCGCCAG
      721 TTGTTGGCCA TACGGATTGT ACCCCTCGAT GCATGGCTGA GATTTGGGCC TTAGCAGTGC
      781 CCTGTCTCTC CACAGTCCAC CCGTAGGGAG CGTCAACGCT TATGATGGAC TCACCCGTTA
      841 TTACGTCAGT AACTGTTCCT GACATGTAGG AGCATCCCAC GGGGGCCGTA AGGCCCTCGA
      901 GCATGTTACC TACAGGTAGG AGCCAGTCGA CAACGAATGA GAAAGGCACC TTTTCCCACA
      961 CTATACCTAG TGGGTTCAAG ATACCTAGAG ACGACAACCA TGCCAAACGT GCATCGTTTA
     1021 TGTAAAACCA TATCACGATA CGTCGCGATA TGTTGCACGT TGTCTGGAAG TTTGCAGCTG
     1081 GATACGACAG ACGGCCATCT AACTTGATGT TAGTACCGAC CTGACGTACG GCTCTCATAG
     1141 GAAGAAACTC TTGAAGGTGA ACCTTCGTAA GCATCTCATA TGCACCCTGG ATATCACTCA
     1201 TTAGTGGTAA CCAACCGAAC TGCAACTCCA ACCACCTGCC GGCCACGTGT TTTGATCGAA
     1261 ACTTTCGATC TTCGTTTAGG GCAAGGTAGC GGAGCGCCTG GCGCCAATTA CCGCGACGAG
     1321 CGGCAGTGTA CGCCTTCACG AGCGCAATGG TTTGCGTCGC GAGTTGTGAG GCTGTCGACC
     1381 TGGCCTCTGC TAAAGCAACA CCAAGGTTAA AATTACCCTG GGTGACCTTT TGCAGGACTT
     1441 CGGTCGACGC CCGGTTCGCA ACGTTCTGCG GCACTTCGAT GTAAGTCAAG TTTTGGCTTA
     1501 CAGGGAAGAG GCTGTAGCAG GAGCGTGCGT CGAGGGAGAA GCCGAAACCG GCTTTCTCCT
     1561 CGTACGGGCG ACCCCACGAT GACCCACTTC GCTTGTAGGC ACCTTGATCT ATCGATGTGA
     1621 CACTTAACGC CCCCCGTGAA TACGGAGAGG GGTAGTGCCA CTGTTTCGTT TTGGCCCCAG
     1681 TCGAGTTAAA ACGACCGGGA GTCCAGTTCG AACGATATTT TAAAGAGAAT GAGTTATCTT
     1741 CAGTCTCACC GTCCGCGTAA ACGCGAACGG AGGGGACGAA GGTCTCGTTC TCCCTATCAA
     1801 GGGTACTAAA AGCTCGCACA GGTCAAACCT CCTAGGAATG GAATTCCGGC TACCTACAGC
     1861 GATAGCCATG GTAGCGTCTC GCTAAAGACA TTAAAAATGG CATTAGCTCG ACAGGAAGTT
     1921 GAGCAGGAGG TACCCAGATC TGGGCTGTCC ATGTGCTGGC GTTCGAATTT AGCAGCAGCG
     1981 GTTTCTTTCA TACCAGAACC GCGTGGCACC AGACCAGAAG AATGATGATG ATGATGGTGC
     2041 ATATGTATAT CTCCTTCTTA AAGTTAAACA AAATTATTTC TAGAGGGGAA TTGTTATCCG
     2101 CTCACAATTC CCCTATAGTG AGTCGTATTA ATTTCGCGGG ATCGAGATCG ATCTCGATCC
     2161 TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT GCTGGCGCCT
     2221 ATATCGCCGA CATCACCGAT GGGGAAGATC GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT
     2281 GTTTCGGCGT GGGTATGGTG GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT
     2341 TGCATGCACC ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT
     2401 TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGAGATCC CGGACACCAT CGAATGGCGC
     2461 AAAACCTTTC GCGGTATGGC ATGATAGCGC CCGGAAGAGA GTCAATTCAG GGTGGTGAAT
     2521 GTGAAACCAG TAACGTTATA CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT
     2581 TCCCGCGTGG TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG
     2641 GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC GGGCAAACAG
     2701 TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC ACGCGCCGTC GCAAATTGTC
     2761 GCGGCGATTA AATCTCGCGC CGATCAACTG GGTGCCAGCG TGGTGGTGTC GATGGTAGAA
     2821 CGAAGCGGCG TCGAAGCCTG TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT
     2881 GGGCTGATCA TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC
     2941 ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA CAGTATTATT
     3001 TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC TGGTCGCATT GGGTCACCAG
     3061 CAAATCGCGC TGTTAGCGGG CCCATTAAGT TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC
     3121 TGGCATAAAT ATCTCACTCG CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG
     3181 AGTGCCATGT CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT
     3241 GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT TACCGAGTCC
     3301 GGGCTGCGCG TTGGTGCGGA CATCTCGGTA GTGGGATACG ACGATACCGA AGACAGCTCA
     3361 TGTTATATCC CGCCGTTAAC CACCATCAAA CAGGATTTTC GCCTGCTGGG GCAAACCAGC
     3421 GTGGACCGCT TGCTGCAACT CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC
     3481 GTCTCACTGG TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC
     3541 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG
     3601 TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG GCACCGGGAT CTCGACCGAT
     3661 GCCCTTGAGA GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT
     3721 CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCT
     3781 CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG ACGATGATCG GCCTGTCGCT
     3841 TGCGGTATTC GGAATCTTGC ACGCCCTCGC TCAAGCCTTC GTCACTGGTC CCGCCACCAA
     3901 ACGTTTCGGC GAGAAGCAGG CCATTATCGC CGGCATGGCG GCCCCACGGG TGCGCATGAT
     3961 CGTGCTCCTG TCGTTGAGGA CCCGGCTAGG CTGGCGGGGT TGCCTTACTG GTTAGCAGAA
     4021 TGAATCACCG ATACGCGAGC GAACGTGAAG CGACTGCTGC TGCAAAACGT CTGCGACCTG
     4081 AGCAACAACA TGAATGGTCT TCGGTTTCCG TGTTTCGTAA AGTCTGGAAA CGCGGAAGTC
     4141 AGCGCCCTGC ACCATTATGT TCCGGATCTG CATCGCAGGA TGCTGCTGGC TACCCTGTGG
     4201 AACACCTACA TCTGTATTAA CGAAGCGCTG GCATTGACCC TGAGTGATTT TTCTCTGGTC
     4261 CCGCCGCATC CATACCGCCA GTTGTTTACC CTCACAACGT TCCAGTAACC GGGCATGTTC
     4321 ATCATCAGTA ACCCGTATCG TGAGCATCCT CTCTCGTTTC ATCGGTATCA TTACCCCCAT
     4381 GAACAGAAAT CCCCCTTACA CGGAGGCATC AGTGACCAAA CAGGAAAAAA CCGCCCTTAA
     4441 CATGGCCCGC TTTATCAGAA GCCAGACATT AACGCTTCTG GAGAAACTCA ACGAGCTGGA
     4501 CGCGGATGAA CAGGCAGACA TCTGTGAATC GCTTCACGAC CACGCTGATG AGCTTTACCG
     4561 CAGCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA
     4621 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC
     4681 AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG TCACGTAGCG ATAGCGGAGT
     4741 GTATACTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATATGC
     4801 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC TCTTCCGCTT
     4861 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
     4921 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
     4981 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
     5041 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
     5101 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
     5161 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
     5221 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
     5281 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
     5341 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
     5401 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
     5461 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
     5521 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
     5581 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
     5641 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAACA
     5701 ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG
     5761 GAAACGTCTT GCTCTAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT
     5821 AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGATT GTATGGGAAG
     5881 CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA
     5941 GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT
     6001 TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG GAAAACAGCA
     6061 TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG
     6121 TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA
     6181 TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT
     6241 GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG
     6301 CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT
     6361 GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC
     6421 CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG
     6481 CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG
     6541 CTCGATGAGT TTTTCTAAGA ATTAATTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA
     6601 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG AAATTGTAAG
     6661 CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA
     6721 ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG
     6781 TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG
     6841 GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT
     6901 TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG
     6961 AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC
     7021 GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC
     7081 GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCA
//
