ID AU2; SV 1; circular; unknown; STD; UNC; 7117 BP. AC unknown; DT 01-DEC.-2020 DE LMBP 7766. OS synthetic DNA construct OC CC Accession number: LMBP 7766 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 1-DEC-2020 CC NGS data implemented FH Key Location/Qualifiers FH FT terminator complement(26..73) FT /label="T7 terminator" FT misc_feature complement(140..157) FT /label="His-tag" FT CDS complement(222..614) FT /label="COAT" FT /note="MS2 sequence encoding the coat protein" FT CDS complement(638..1819) FT /label="A" FT /note="MS2 sequence encoding the assembly protein" FT misc_feature complement(1944..1988) FT /label="S-tag" FT misc_feature complement(1995..2012) FT /label="thrombin recognition site" FT misc_feature complement(2022..2039) FT /label="His-tag" FT RBS complement(2043..2087) FT /label="5' UTR of T7 gene 10, incl. RBS" FT promoter complement(2089..2130) FT /label="T7 promoter with lac operator" FT promoter 2443..2520 FT /label="lacI promoter, incl. RBS" FT CDS 2521..3603 FT /label="lacI" FT CDS 4412..4603 FT /label="rop" FT rep_origin complement(4849..5251) FT /label="pMB1 ori" FT CDS 5743..6558 FT /label="Tn903 neomycin resistance" FT rep_origin complement(6651..7106) FT /label="f1 ori" SQ SEQUENCE 7117 AA; 2200461 MW; ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA 60 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT 120 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGTGCGGCC GCAAGCTTTC 180 TTCGACATGG GTAATCCTCA TGTTTGAATG GCCGGCGTCT ATTAGTAGAT GCCGGAGTTT 240 GCTGCGATTG CTGAGGGAAT CGGGTTTCCA TCTTTTAGGA GACCTTGCAT TGCCTTAACA 300 ATAAGCTCGC AGTCGGAATT CGTGGCGAAA ATTGGAATGG TTAGTTCCAT ATTTAAGTAC 360 GAACGCCATG CGGCTACAGG AAGCTCTACA CCACCAACAG TCTGGGTTGC CACTTTAGGC 420 ACCTCGACTT TGATGGTGTA TTTGCGATTC TGCGCAGAGC TCTGACGAAC GCTACAGGTT 480 ACTTTGTAAG CCTGTGAACG CGAGTTAGAG CTGATCCATT CAGCGACCCC GTTAGCGAAG 540 TTGCTTGGGG CGACAGTCAC GTCGCCAGTT CCGCCATTGT CGACGAGAAC GAACTGAGTA 600 AAGTTAGAAG CCATGCTTCA AACTCCGGTT GAGGGCTCTA TCTAGAGAGC CGTTGCCTGA 660 TTAATGCTAA CGCATCTAAG GTATGGACCA TCGAGAAAGG AGACTTTACG TACGCGCCAG 720 TTGTTGGCCA TACGGATTGT ACCCCTCGAT GCATGGCTGA GATTTGGGCC TTAGCAGTGC 780 CCTGTCTCTC CACAGTCCAC CCGTAGGGAG CGTCAACGCT TATGATGGAC TCACCCGTTA 840 TTACGTCAGT AACTGTTCCT GACATGTAGG AGCATCCCAC GGGGGCCGTA AGGCCCTCGA 900 GCATGTTACC TACAGGTAGG AGCCAGTCGA CAACGAATGA GAAAGGCACC TTTTCCCACA 960 CTATACCTAG TGGGTTCAAG ATACCTAGAG ACGACAACCA TGCCAAACGT GCATCGTTTA 1020 TGTAAAACCA TATCACGATA CGTCGCGATA TGTTGCACGT TGTCTGGAAG TTTGCAGCTG 1080 GATACGACAG ACGGCCATCT AACTTGATGT TAGTACCGAC CTGACGTACG GCTCTCATAG 1140 GAAGAAACTC TTGAAGGTGA ACCTTCGTAA GCATCTCATA TGCACCCTGG ATATCACTCA 1200 TTAGTGGTAA CCAACCGAAC TGCAACTCCA ACCACCTGCC GGCCACGTGT TTTGATCGAA 1260 ACTTTCGATC TTCGTTTAGG GCAAGGTAGC GGAGCGCCTG GCGCCAATTA CCGCGACGAG 1320 CGGCAGTGTA CGCCTTCACG AGCGCAATGG TTTGCGTCGC GAGTTGTGAG GCTGTCGACC 1380 TGGCCTCTGC TAAAGCAACA CCAAGGTTAA AATTACCCTG GGTGACCTTT TGCAGGACTT 1440 CGGTCGACGC CCGGTTCGCA ACGTTCTGCG GCACTTCGAT GTAAGTCAAG TTTTGGCTTA 1500 CAGGGAAGAG GCTGTAGCAG GAGCGTGCGT CGAGGGAGAA GCCGAAACCG GCTTTCTCCT 1560 CGTACGGGCG ACCCCACGAT GACCCACTTC GCTTGTAGGC ACCTTGATCT ATCGATGTGA 1620 CACTTAACGC CCCCCGTGAA TACGGAGAGG GGTAGTGCCA CTGTTTCGTT TTGGCCCCAG 1680 TCGAGTTAAA ACGACCGGGA GTCCAGTTCG AACGATATTT TAAAGAGAAT GAGTTATCTT 1740 CAGTCTCACC GTCCGCGTAA ACGCGAACGG AGGGGACGAA GGTCTCGTTC TCCCTATCAA 1800 GGGTACTAAA AGCTCGCACA GGTCAAACCT CCTAGGAATG GAATTCCGGC TACCTACAGC 1860 GATAGCCATG GTAGCGTCTC GCTAAAGACA TTAAAAATGG CATTAGCTCG ACAGGAAGTT 1920 GAGCAGGAGG TACCCAGATC TGGGCTGTCC ATGTGCTGGC GTTCGAATTT AGCAGCAGCG 1980 GTTTCTTTCA TACCAGAACC GCGTGGCACC AGACCAGAAG AATGATGATG ATGATGGTGC 2040 ATATGTATAT CTCCTTCTTA AAGTTAAACA AAATTATTTC TAGAGGGGAA TTGTTATCCG 2100 CTCACAATTC CCCTATAGTG AGTCGTATTA ATTTCGCGGG ATCGAGATCG ATCTCGATCC 2160 TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT GCTGGCGCCT 2220 ATATCGCCGA CATCACCGAT GGGGAAGATC GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT 2280 GTTTCGGCGT GGGTATGGTG GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT 2340 TGCATGCACC ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT 2400 TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGAGATCC CGGACACCAT CGAATGGCGC 2460 AAAACCTTTC GCGGTATGGC ATGATAGCGC CCGGAAGAGA GTCAATTCAG GGTGGTGAAT 2520 GTGAAACCAG TAACGTTATA CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT 2580 TCCCGCGTGG TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG 2640 GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC AACAACTGGC GGGCAAACAG 2700 TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC ACGCGCCGTC GCAAATTGTC 2760 GCGGCGATTA AATCTCGCGC CGATCAACTG GGTGCCAGCG TGGTGGTGTC GATGGTAGAA 2820 CGAAGCGGCG TCGAAGCCTG TAAAGCGGCG GTGCACAATC TTCTCGCGCA ACGCGTCAGT 2880 GGGCTGATCA TTAACTATCC GCTGGATGAC CAGGATGCCA TTGCTGTGGA AGCTGCCTGC 2940 ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA CACCCATCAA CAGTATTATT 3000 TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC TGGTCGCATT GGGTCACCAG 3060 CAAATCGCGC TGTTAGCGGG CCCATTAAGT TCTGTCTCGG CGCGTCTGCG TCTGGCTGGC 3120 TGGCATAAAT ATCTCACTCG CAATCAAATT CAGCCGATAG CGGAACGGGA AGGCGACTGG 3180 AGTGCCATGT CCGGTTTTCA ACAAACCATG CAAATGCTGA ATGAGGGCAT CGTTCCCACT 3240 GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA TGCGCGCCAT TACCGAGTCC 3300 GGGCTGCGCG TTGGTGCGGA CATCTCGGTA GTGGGATACG ACGATACCGA AGACAGCTCA 3360 TGTTATATCC CGCCGTTAAC CACCATCAAA CAGGATTTTC GCCTGCTGGG GCAAACCAGC 3420 GTGGACCGCT TGCTGCAACT CTCTCAGGGC CAGGCGGTGA AGGGCAATCA GCTGTTGCCC 3480 GTCTCACTGG TGAAAAGAAA AACCACCCTG GCGCCCAATA CGCAAACCGC CTCTCCCCGC 3540 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG 3600 TGAGCGCAAC GCAATTAATG TAAGTTAGCT CACTCATTAG GCACCGGGAT CTCGACCGAT 3660 GCCCTTGAGA GCCTTCAACC CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT 3720 CGCCGCACTT ATGACTGTCT TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCT 3780 CTGGGTCATT TTCGGCGAGG ACCGCTTTCG CTGGAGCGCG ACGATGATCG GCCTGTCGCT 3840 TGCGGTATTC GGAATCTTGC ACGCCCTCGC TCAAGCCTTC GTCACTGGTC CCGCCACCAA 3900 ACGTTTCGGC GAGAAGCAGG CCATTATCGC CGGCATGGCG GCCCCACGGG TGCGCATGAT 3960 CGTGCTCCTG TCGTTGAGGA CCCGGCTAGG CTGGCGGGGT TGCCTTACTG GTTAGCAGAA 4020 TGAATCACCG ATACGCGAGC GAACGTGAAG CGACTGCTGC TGCAAAACGT CTGCGACCTG 4080 AGCAACAACA TGAATGGTCT TCGGTTTCCG TGTTTCGTAA AGTCTGGAAA CGCGGAAGTC 4140 AGCGCCCTGC ACCATTATGT TCCGGATCTG CATCGCAGGA TGCTGCTGGC TACCCTGTGG 4200 AACACCTACA TCTGTATTAA CGAAGCGCTG GCATTGACCC TGAGTGATTT TTCTCTGGTC 4260 CCGCCGCATC CATACCGCCA GTTGTTTACC CTCACAACGT TCCAGTAACC GGGCATGTTC 4320 ATCATCAGTA ACCCGTATCG TGAGCATCCT CTCTCGTTTC ATCGGTATCA TTACCCCCAT 4380 GAACAGAAAT CCCCCTTACA CGGAGGCATC AGTGACCAAA CAGGAAAAAA CCGCCCTTAA 4440 CATGGCCCGC TTTATCAGAA GCCAGACATT AACGCTTCTG GAGAAACTCA ACGAGCTGGA 4500 CGCGGATGAA CAGGCAGACA TCTGTGAATC GCTTCACGAC CACGCTGATG AGCTTTACCG 4560 CAGCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA 4620 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC 4680 AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG TCACGTAGCG ATAGCGGAGT 4740 GTATACTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATATGC 4800 GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC TCTTCCGCTT 4860 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT 4920 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG 4980 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 5040 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 5100 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 5160 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 5220 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 5280 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 5340 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 5400 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 5460 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 5520 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 5580 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 5640 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAACA 5700 ATAAAACTGT CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG 5760 GAAACGTCTT GCTCTAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT 5820 AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGATT GTATGGGAAG 5880 CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA TGATGTTACA 5940 GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC CATCAAGCAT 6000 TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG GAAAACAGCA 6060 TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG 6120 TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG CGATCGCGTA 6180 TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC GAGTGATTTT 6240 GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA TAAACTTTTG 6300 CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT 6360 GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC 6420 CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT ACAGAAACGG 6480 CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT TCATTTGATG 6540 CTCGATGAGT TTTTCTAAGA ATTAATTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 6600 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG AAATTGTAAG 6660 CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA 6720 ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG 6780 TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG 6840 GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT 6900 TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG 6960 AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC 7020 GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC 7080 GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCA 7117 //