LOCUS       pCAGGS-NLS-Cre-PGK-Puro        7632 bp    DNA     circular     21-AUG-2012
DEFINITION  LMBP 7779.
SOURCE      
  ORGANISM  
COMMENT     Accesson number: LMBP 7779
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 21-AUG-2012
COMMENT     VNTDATE|630942543|
COMMENT     VNTNAME|pCAGGS-NLS-Cre-PGK-Puro|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAGGS-NLS-Cre-PGK-Puro" 0 7632 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AgeI" "EcoRI" "EcoRV" "HincII" "NotI" "PvuI" "SacII" 
COMMENT         "XbaI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 2748 2747 1 7632 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 2 118 0 1828 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1708 0 7631 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 4 628 0 1132 0 2871 0 3147 
COMMENT            0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HincII" "gtyrac" 4 0 1 5905 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3387 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 535 0 5353 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 4 2478 0 3393 0 6937 0 
COMMENT            7466 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 7535 0 0 "")) 
COMMENT       (CObList 
COMMENT        #8=(CFSignal (CObList) "3' UTR of rabbit \337-globin, incl. polyA" 43 0 
COMMENT            0 1220 1311 0 (CStringList) (CStringList) 1 1 1 1 "1220..1311") 
COMMENT        #9=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 0 
COMMENT            1312 1668 0 (CStringList) (CStringList) 1 1 1 1 "1312..1668") 
COMMENT        #10=(CFSignal (CObList) "lac promoter" 30 0 1 3448 3530 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3449..3531)") 
COMMENT        #11=(CFSignal (CObList) "SV40 ori" 33 0 0 3672 3747 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3673..3748") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3778 3852 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3779..3853") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3778 3852 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3779..3853)") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 1 3967 4368 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3968..4369)") 
COMMENT        #15=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4909 5769 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4910..5770)") 
COMMENT        #16=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 5905 6282 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5906..6283") 
COMMENT        #17=(CFSignal (CObList) "AG promoter" 29 0 0 6288 7629 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6289..7630") 
COMMENT        #18=(CFSignal (CObList) "intron AG promoter" 15 0 0 6657 7581 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6658..7582") 
COMMENT        #19=(CFSignal (CObList) "cre" 4 0 0 79 1107 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "79..1107") 
COMMENT        #20=(CFSignal (CObList) "SV40 large T NLS" 4 0 0 58 78 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "52..78") 
COMMENT        #21=(CFSignal (CObList) "puromycin resistance" 4 0 0 2263 2862 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2264..2863") 
COMMENT        #22=(CFSignal (CObList) "3' UTR of mPGK1, incl. polyA" 43 0 0 2936 3214 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "2937..3215") 
COMMENT        #23=(CFSignal (CObList) "mPGK1 promoter" 29 0 0 1710 2217 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #24=(CFSignal (CObList) "LMBP 7779" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #25=(CFSignal (CObList) "Last modified: 21-AUG-2012" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                    "pCAGGS-NLS-Cre-PGK-Puro" 2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'pCAGGS-NLS-Cre-PGK-Puro'" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 7779." 
COMMENT                          1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 07/06/12 01:49" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 08/21/12 01:29" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7632 bp" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                    (CObjectList 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 20-AUG-2012" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #40=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #41=(CFolderPar 
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COMMENT                     #42=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 7779") 
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COMMENT                           (CObjectList 
COMMENT                            #45=(CLinePar (CParagraph 0 (15 #25# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 21-AUG-2012"))) 
COMMENT                   "Annotations") 
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COMMENT                    (CObjectList 
COMMENT                     #47=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #48=(CFolderPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 58    End: 78  " 1) 
COMMENT                                   #50=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   52..78" 1))) "SV40 large T NLS") 
COMMENT                            #52=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 79 (3 #19# 0) 1 2 2 0 327) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 79    End: 1107" 1) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   79..1107" 1))) "cre") 
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COMMENT                                        "Start: 2263  End: 2862" 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2264..2863" 1))) 
COMMENT                                 "puromycin resistance") 
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COMMENT                                        "Start: 4909  End: 5769 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4910..5770)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (4 total)") 
COMMENT                     #64=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 15 (7 15 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #65=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6657  End: 7581" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid intron" 
COMMENT                                        1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   6658..7582" 1))) 
COMMENT                                 "intron AG promoter"))) "Intron (1 total)") 
COMMENT                     #70=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #71=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1312 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1312  End: 1668" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1312..1668" 1))) 
COMMENT                                 "3' flanking region of rabbit \337-globin"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #75=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1710 (3 #23# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1710  End: 2217" 1))) 
COMMENT                                 "mPGK1 promoter") 
COMMENT                            #78=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5905 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5905  End: 6282" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human CMV-IE enhancer" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5906..6283" 1))) "hCMV-IE enhancer") 
COMMENT                            #83=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 6288 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 6288  End: 7629" 1) 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chicken \337-actin/rabbit \337-globin hybrid promoter" 
COMMENT                                        1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   6289..7630" 1))) "AG promoter"))) 
COMMENT                          "Promoter Eukaryotic (3 total)") 
COMMENT                     #88=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #89=(CFolderPar 
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COMMENT                                        "Start: 3448  End: 3530 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "E. coli lac operon promoter" 1) 
COMMENT                                   #92=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3449..3531)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "Start: 3672  End: 3747" 1) 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "bidirectional origin of SV40" 1) 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "   3673..3748" 1))) "SV40 ori") 
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COMMENT                                          "origin plasmid" 1) 
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COMMENT                                          "   complement(3968..4369)" 1))) 
COMMENT                                  "pMB1 ori"))) "Replication Origin (2 total)") 
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COMMENT                                           "   2937..3215" 1))) 
COMMENT                                   "3' UTR of mPGK1, incl. polyA") 
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COMMENT                                           "Start: 3778  End: 3852" 1) 
COMMENT                                     #116=(CLinePar 
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COMMENT                                           "polyA signal of the SV40 early region" 
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COMMENT                                           "   3779..3853" 1))) 
COMMENT                                   "SV40 polyA early") 
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COMMENT                                           "polyA signal of the SV40 late region" 
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COMMENT                                           "   complement(3779..3853)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (4 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                                            " N1: 5905 " 1))))) "HincII: 1 site") 
COMMENT                            3 3 "GTYRAC" "CARYTG") 
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COMMENT                                            " N1: 3387 " 1))))) "NotI: 1 site") 
COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                                            " N1: 7535 " 1))))) "XbaI: 1 site") 
COMMENT                            1 5 "TCTAGA" "AGATCT") 
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COMMENT                                            " N1: 118 " 1) 
COMMENT                                      #137=(CLinePar 
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FEATURES             Location/Qualifiers
     terminator      1220..1311
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    1312..1668
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(3448..3530)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      3672..3747
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      3778..3852
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(3778..3852)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(3967..4368)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(4909..5769)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        5905..6282
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        6288..7629
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          6657..7581
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
     CDS             79..1107
                     /vntifkey="4"
                     /label=cre
     CDS             58..78
                     /vntifkey="4"
                     /label=SV40\large\T\NLS
     CDS             2263..2862
                     /vntifkey="4"
                     /label=puromycin\resistance
     terminator      2936..3214
                     /vntifkey="43"
                     /label=3'\UTR\of\mPGK1,\incl.\polyA
     promoter        1710..2217
                     /vntifkey="29"
                     /label=mPGK1\promoter
BASE COUNT     1553 a      1985 c      2078 g      1720 t       296 others
ORIGIN
        1 ttcgacctgc agcccaagct gtaccccctc gaaactgaca ggagaaccac catgggccca 
       61 aagaagaaga gaaaggtttc caatttactg accgtacacc aaaatttgcc tgcattaccg 
      121 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag 
      181 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg 
      241 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt 
      301 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta 
      361 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg 
      421 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta 
      481 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc 
      541 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc 
      601 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc 
      661 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg 
      721 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat 
      781 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag 
      841 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct 
      901 aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc 
      961 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg 
     1021 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg 
     1081 gtgcgcctgc tggaagatgg cgattagcca ttaacgcgcg accagcttga tatcgaatta 
     1141 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct 
     1201 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa 
     1261 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg 
     1321 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca 
     1381 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa 
     1441 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt 
     1501 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt 
     1561 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct 
     1621 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatcnn nnnnnnnnnn 
     1681 nnnnnnnnnn nnnnnngagc tcnnnngaat tctaccgggt aggggaggcg cttttcccaa 
     1741 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc 
     1801 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg 
     1861 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc 
     1921 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg 
     1981 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt 
     2041 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg 
     2101 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc 
     2161 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgannn 
     2221 nnnnnnnnnn nnnnnnnnnc tcgagcagct gnnaagcttn nnatgaccga gtacaagccc 
     2281 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 
     2341 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 
     2401 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 
     2461 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 
     2521 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 
     2581 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 
     2641 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 
     2701 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 
     2761 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 
     2821 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gannnnngat atcnnnnnnn 
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagaaa 
     2941 ttgatgatct attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt 
     3001 tgttaagaag ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg 
     3061 gtgggggtgg ggtgggatta gataaatgcc tgctctttac tgaaggctct ttactattgc 
     3121 tttatgataa tgtttcatag ttggatatca taatttaaac aagcaaaacc aaattaaggg 
     3181 ccagctcatt cctcccactc atgatctata gatcnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
     3361 nnnnnnnnnn nnnnnnnnnn nnnngcggcc gcggcgtaat catggtcata gctgtttcct 
     3421 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 
     3481 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     3541 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat 
     3601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     3661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     3721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac 
     3781 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     3841 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 
     3901 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     3961 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     4021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc 
     4081 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 
     4141 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 
     4201 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 
     4261 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 
     4321 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 
     4381 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 
     4441 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 
     4501 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 
     4561 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 
     4621 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 
     4681 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 
     4741 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 
     4801 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 
     4861 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 
     4921 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 
     4981 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 
     5041 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 
     5101 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 
     5161 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 
     5221 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 
     5281 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 
     5341 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 
     5401 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 
     5461 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 
     5521 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 
     5581 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 
     5641 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 
     5701 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 
     5761 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 
     5821 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 
     5881 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa 
     5941 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 
     6001 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     6061 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     6121 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     6181 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     6241 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca 
     6301 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 
     6361 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg 
     6421 gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc 
     6481 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 
     6541 aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct 
     6601 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga 
     6661 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg 
     6721 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg 
     6781 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg 
     6841 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg 
     6901 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga 
     6961 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg 
     7021 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg 
     7081 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc 
     7141 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg 
     7201 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 
     7261 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 
     7321 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 
     7381 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 
     7441 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 
     7501 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 
     7561 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 
     7621 tttggcaaag aa 
//
