ID pCAGGS-NLS-Cre-PGK-Puro standard; circular DNA; ; 7632 BP. DE LMBP 7779. CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 21-AUG-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator 1220..1311 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT misc_feature 1312..1668 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT promoter complement(3448..3530) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin 3672..3747 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT terminator 3778..3852 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(3778..3852) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(3967..4368) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(4909..5769) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter 5905..6282 FT /vntifkey="29" FT /label=hCMV-IE\enhancer FT /note="human CMV-IE enhancer" FT promoter 6288..7629 FT /vntifkey="29" FT /label=AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid promoter" FT intron 6657..7581 FT /vntifkey="15" FT /label=intron\AG\promoter FT /note="chicken ß-actin/rabbit ß-globin hybrid intron" FT CDS 79..1107 FT /vntifkey="4" FT /label=cre FT CDS 58..78 FT /vntifkey="4" FT /label=SV40\large\T\NLS FT CDS 2263..2862 FT /vntifkey="4" FT /label=puromycin\resistance FT terminator 2936..3214 FT /vntifkey="43" FT /label=3'\UTR\of\mPGK1,\incl.\polyA FT promoter 1710..2217 FT /vntifkey="29" FT /label=mPGK1\promoter SQ Sequence 7632 BP; 1553 A; 1985 C; 2078 G; 1720 t; 296 other; ttcgacctgc agcccaagct gtaccccctc gaaactgaca ggagaaccac catgggccca 60 aagaagaaga gaaaggtttc caatttactg accgtacacc aaaatttgcc tgcattaccg 120 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag 180 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg 240 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt 300 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta 360 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg 420 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta 480 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc 540 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc 600 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc 660 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg 720 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat 780 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag 840 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct 900 aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc 960 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg 1020 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg 1080 gtgcgcctgc tggaagatgg cgattagcca ttaacgcgcg accagcttga tatcgaatta 1140 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct 1200 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa 1260 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg 1320 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca 1380 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa 1440 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt 1500 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt 1560 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct 1620 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatcnn nnnnnnnnnn 1680 nnnnnnnnnn nnnnnngagc tcnnnngaat tctaccgggt aggggaggcg cttttcccaa 1740 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc 1800 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg 1860 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc 1920 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg 1980 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt 2040 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg 2100 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc 2160 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgannn 2220 nnnnnnnnnn nnnnnnnnnc tcgagcagct gnnaagcttn nnatgaccga gtacaagccc 2280 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 2340 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 2400 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 2460 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 2520 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 2580 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 2640 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 2700 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 2760 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 2820 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gannnnngat atcnnnnnnn 2880 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnagaaa 2940 ttgatgatct attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt 3000 tgttaagaag ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg 3060 gtgggggtgg ggtgggatta gataaatgcc tgctctttac tgaaggctct ttactattgc 3120 tttatgataa tgtttcatag ttggatatca taatttaaac aagcaaaacc aaattaaggg 3180 ccagctcatt cctcccactc atgatctata gatcnnnnnn nnnnnnnnnn nnnnnnnnnn 3240 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3300 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 3360 nnnnnnnnnn nnnnnnnnnn nnnngcggcc gcggcgtaat catggtcata gctgtttcct 3420 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 3480 aaagcctggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 3540 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat 3600 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 3660 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 3720 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac 3780 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 3840 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 3900 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 3960 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 4020 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca gggataacgc 4080 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 4140 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 4200 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 4260 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 4320 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 4380 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 4440 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 4500 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 4560 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 4620 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 4680 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4740 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4800 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4860 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 4920 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 4980 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 5040 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 5100 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 5160 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 5220 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 5280 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5340 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5400 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 5460 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 5520 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5580 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5640 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5700 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5760 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 5820 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 5880 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa 5940 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa 6000 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 6060 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 6120 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 6180 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 6240 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca 6300 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta 6360 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg 6420 gggcggggcg gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc 6480 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 6540 aaaagcgaag cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct 6600 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga 6660 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg 6720 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg 6780 ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg 6840 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg 6900 tgcgcgaggg gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga 6960 acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg 7020 tcgggctgca accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg 7080 gtgcggggct ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc 7140 aggtgggggt gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg 7200 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt 7260 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg 7320 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc 7380 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc 7440 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg 7500 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat 7560 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat 7620 tttggcaaag aa 7632 //