LOCUS       pBluescriptIISK(-)-IRES-LacZ        6830 bp    DNA     circular      2-NOV-2012
DEFINITION  LMBP 7845.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7845
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 2-NOV-2012
            
COMMENT     VNTDATE|641635040|
COMMENT     VNTNAME|pBluescriptIISK(-)-IRES-LacZ|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT            4583 0 0 "") 
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COMMENT            4253 0 6036 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 4581 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 817 0 0 "") 
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COMMENT            (CStringList) 1 1 1 1 "626..642") 
COMMENT        #8=(CFSignal (CObList) "T3 promoter" 30 0 1 4640 4659 0 (CStringList) 
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COMMENT        #231=(CSampFrag "pBluescriptIISK(-)-IRES-LacZ-)-rob" 2916 4583 "AvaI" 669 "AvaI" 
COMMENT              (LOGPEN 0 0 0) 6.46578e+170 1.05744e-301) 
COMMENT        #232=(CSampFrag "pBluescriptIISK(-)-IRES-LacZ-)-rob" 1959 2624 "AvaI" 4583 "AvaI" 
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FEATURES             Location/Qualifiers
     CDS             complement(5842..6702)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      4..459
                     /vntifkey="33"
                     /label=f1\ori
     promoter        626..642
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(4640..4659)
                     /vntifkey="30"
                     /label=T3\promoter
     RBS             complement(4686..4723)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(4724..4807)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(4899..5301)
                     /vntifkey="33"
                     /label=pMB1\ori
     RBS             complement(3979..4552)
                     /vntifkey="32"
                     /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES)
     CDS             complement(909..3968)
                     /vntifkey="4"
                     /label=lacZ\mutant
BASE COUNT     1554 a      1857 c      1810 g      1609 t 
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 
      661 gccccccctc gagtgggtgg tgagcagctc gccgtcgcgc agcaggcgcc agcgctccat 
      721 ccacgcagtg acctcaggcg gaaaacggga agacacactc atgagatgcc tgcaagcaat 
      781 tcgttctgta tcaggcgcag gagcgtcccg tccgggtcga ctcgagcaaa ttcgagctcg 
      841 cccaaatagt acataatgga tttccttacg cgaaatacgg gcagacatgg cctgcccggt 
      901 tattattatt tttgacacca gaccaactgg taatggtagc gaccggcgct cagctggaat 
      961 tccgccgata ctgacgggct ccaggagtcg tcgccaccaa tccccatatg gaaaccgtcg 
     1021 atattcagcc atgtgccttc ttccgcgtgc agcagatggc gatggctggt ttccatcagt 
     1081 tgctgttgac tgtagcggct gatgttgaac tggaagtcgc cgcgccactg gtgtgggcca 
     1141 taattcaatt cgcgcgtccc gcagcgcaga ccgttttcgc tcgggaagac gtacggggta 
     1201 tacatgtctg acaatggcag atcccagcgg tcaaaacagg cggcagtaag gcggtcggga 
     1261 tagttttctt gcggccctaa tccgagccag tttacccgct ctgctacctg cgccagctgg 
     1321 cagttcaggc caatccgcgc cggatgcggt gtatcgctcg ccacttcaac atcaacggta 
     1381 atcgccattt gaccactacc atcaatccgg taggttttcc ggctgataaa taaggttttc 
     1441 ccctgatgct gccacgcgtg agcggtcgta atcagcaccg catcagcaag tgtatctgcc 
     1501 gtgcactgca acaacgctgc ttcggcctgg taatggcccg ccgccttcca gcgttcgacc 
     1561 caggcgttag ggtcaatgcg ggtcgcttca cttacgccaa tgtcgttatc cagcggtgca 
     1621 cgggtgaact gatcgcgcag cggcgtcagc agttgttttt tatcgccaat ccacatctgt 
     1681 gaaagaaagc ctgactggcg gttaaattgc caacgcttat tacccagctc gatgcaaaaa 
     1741 tccatttcgc tggtggtcag atgcgggatg gcgtgggacg cggcggggag cgtcacactg 
     1801 aggttttccg ccagacgcca ctgctgccag gcgctgatgt gcccggcttc tgaccatgcg 
     1861 gtcgcgttcg gttgcactac gcgtactgtg agccagagtt gcccggcgct ctccggctgc 
     1921 ggtagttcag gcagttcaat caactgttta ccttgtggag cgacatccag aggcacttca 
     1981 ccgcttgcca gcggcttacc atccagcgcc accatccagt gcaggagctc gttatcgcta 
     2041 tgacggaaca ggtattcgct ggtcacttcg atggtttgcc cggataaacg gaactggaaa 
     2101 aactgctgct ggtgttttgc ttccgtcagc gctggatgcg gcgtgcggtc ggcaaagacc 
     2161 agaccgttca tacagaactg gcgatcgttc ggcgtatcgc caaaatcacc gccgtaagcc 
     2221 gaccacgggt tgccgttttc atcatattta atcagcgact gatccaccca gtcccagacg 
     2281 aagccgccct gtaaacgggg atactgacga aacgcctgcc agtatttagc gaaaccgcca 
     2341 agactgttac ccatcgcgtg ggcgtattcg caaaggatca gcgggcgcgt ctctccaggt 
     2401 agcgaaagcc attttttgat ggaccatttc ggcacagccg ggaagggctg gtcttcatcc 
     2461 acgcgcgcgt acatcgggca aataatatcg gtggccgtgg tgtcggctcc gccgccttca 
     2521 tactgcaccg ggcgggaagg atcgacagat ttgatccagc gatacagcgc gtcgtgatta 
     2581 gcgccgtggc ctgattcatt ccccagcgac cagatgatca cactcgggtg attacgatcg 
     2641 cgctgcacca ttcgcgttac gcgttcgctc atcgccggta gccagcgcgg atcatcggtc 
     2701 agacgattca ttggcaccat gccgtgggtt tcaatattgg cttcatccac cacatacagg 
     2761 ccgtagcggt cgcacagcgt gtaccacagc ggatggttcg gataatgcga acagcgcacg 
     2821 gcgttaaagt tgttctgctt catcagcagg atatcctgca ccatcgtctg ctcatccatg 
     2881 acctgaccat gcagaggatg atgctcgtga cggttaacgc ctcgaatcag caacggcttg 
     2941 ccgttcagca gcagcagacc attttcaatc cgcacctcgc ggaaaccgac atcgcaggct 
     3001 tctgcttcaa tcagcgtgcc gtcggcggtg tgcagttcaa ccaccgcacg atagagattc 
     3061 gggatttcgg cgctccacag tttcgggttt tcgacgttca gacgtagtgt gacgcgatcg 
     3121 gcataaccac cacgctcatc gataatttca ccgccgaaag gcgcggtgcc gctggcgacc 
     3181 tgcgtttcac cctgccataa agaaactgtt acccgtaggt agtcacgcaa ctcgccgcac 
     3241 atctgaactt cagcctccag tacagcgcgg ctgaaatcat cattaaagcg agtggcaaca 
     3301 tggaaatcgc tgatttgtgt agtcggttta tgcagcaacg agacgtcacg gaaaatgccg 
     3361 ctcatccgcc acatatcctg atcttccaga taactgccgt cactccagcg cagcaccatc 
     3421 accgcgaggc ggttttctcc ggcgcgtaaa aatgcgctca ggtcaaattc agacggcaaa 
     3481 cgactgtcct ggccgtaacc gacccagcgc ccgttgcacc acagatgaaa cgccgagtta 
     3541 acgccatcaa aaataattcg cgtctggcct tcctgtagcc agctttcatc aacattaaat 
     3601 gtgagcgagt aacaacccgt cggattctcc gtgggaacaa acggcggatt gaccgtaatg 
     3661 ggataggtca cgttggtgta gatgggcgca tcgtaaccgt gcatctgcca gtttgagggg 
     3721 acgacgacag tatcggcctc aggaagatcg cactccagcc agctttccgg caccgcttct 
     3781 ggtgccggaa accaggcaaa gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 
     3841 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 
     3901 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacgggatct 
     3961 tccatggttg tggcaagctt atcatcgtgt ttttcaaagg aaaaccacgt ccccgtggtt 
     4021 cggggggcct agacgttttt ttaacctcga ctaaacacat gtaaagcatg tgcaccgagg 
     4081 ccccagatca gatcccatac aatggggtac cttctgggca tccttcagcc ccttgttgaa 
     4141 tacgcttgag gagagccatt tgactctttc cacaactatc caactcacaa cgtggcactg 
     4201 gggttgtgcc gcctttgcag gtgtatctta tacacgtggc ttttggccgc agaggcacct 
     4261 gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg tttgtcttca 
     4321 agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca ttcctttggc 
     4381 gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt tccgggccct 
     4441 cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg cacaccggcc 
     4501 ttattccaag cggcttcggc cagtaacgtt aggggggggg gagggagagg gggggggggg 
     4561 gggggggcgg aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg 
     4621 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg 
     4681 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 
     4741 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 
     4801 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 
     4861 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 
     4921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 
     4981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 
     5041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 
     5101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 
     5161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 
     5221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 
     5281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 
     5341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 
     5401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 
     5461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 
     5521 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 
     5581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 
     5641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 
     5701 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 
     5761 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 
     5821 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 
     5881 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 
     5941 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 
     6001 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 
     6061 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 
     6121 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 
     6181 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 
     6241 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 
     6301 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 
     6361 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 
     6421 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 
     6481 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 
     6541 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 
     6601 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 
     6661 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 
     6721 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 
     6781 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 
//
