LOCUS       pCD-F-MLT_DD-Ig2        6483 bp    DNA     circular     28-SEP-2012
DEFINITION  LMBP 7866.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|638374008|
COMMENT     VNTNAME|pCD-F-MLT_DD-Ig2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCD-F-MLT_DD-Ig2" 0 6483 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 1 0 10 10 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "PmeI" "ApaI" "KpnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 4274 4969 1 6483 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #0=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 2 4259 0 5412 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 5338 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 4305 0 0 "")) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "flag epitope" 21 0 0 4274 4300 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4274..4300") 
COMMENT        #4=(CFSignal (CObList) "2506" 27 0 0 4217 4236 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4217..4236") 
COMMENT        #5=(CFSignal (CObList) "AmpR" 22 0 1 2358 3219 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2358..3219)") 
COMMENT        #6=(CFSignal (CObList) "ColE1" 33 0 0 1540 2213 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1540..2213") 
COMMENT        #7=(CFSignal (CObList) "SV40 polyA" 25 0 0 869 1108 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "869..1108") 
COMMENT        #8=(CFSignal (CObList) "NeoR" 86 0 0 59 853 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "59..853") 
COMMENT        #9=(CFSignal (CObList) "T7" 30 0 0 4217 4236 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4217..4236") 
COMMENT        #10=(CFSignal (CObList) "pCMV" 29 0 0 3563 4217 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3563..4217") 
COMMENT        #11=(CFSignal (CObList) "f1" 33 0 0 5703 6116 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7659..8072") 
COMMENT        #12=(CFSignal (CObList) "BGH pA" 25 0 0 5426 5640 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7382..7596") 
COMMENT        #13=(CFSignal (CObList) "6His" 21 0 0 5387 5404 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7343..7360") 
COMMENT        #14=(CFSignal (CObList) "Myc" 87 0 0 5342 5371 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "MALT1 DD-Ig2" 4 0 0 4307 5332 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: Jens Staal" 
COMMENT                          1) 
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COMMENT                          "Created: 09/28/12 02:06" 1) 
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COMMENT                          "Last Modified: 09/28/12 02:08" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6483 bp" 1) 
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FEATURES             Location/Qualifiers
     misc_feature    4274..4300
                     /vntifkey="21"
                     /label=flag\epitope
     primer          4217..4236
                     /vntifkey="27"
                     /label=2506
     misc_marker     complement(2358..3219)
                     /vntifkey="22"
                     /label=AmpR
     rep_origin      1540..2213
                     /vntifkey="33"
                     /label=ColE1
     polyA_signal    869..1108
                     /vntifkey="25"
                     /label=SV40\polyA
     misc_recomb     59..853
                     /vntifkey="86"
                     /label=NeoR
     promoter        4217..4236
                     /vntifkey="30"
                     /label=T7
     promoter        3563..4217
                     /vntifkey="29"
                     /label=pCMV
     rep_origin      5703..6116
                     /vntifkey="33"
                     /label=f1
     polyA_signal    5426..5640
                     /vntifkey="25"
                     /label=BGH\pA
     misc_feature    5387..5404
                     /vntifkey="21"
                     /label=6His
     misc_signal     5342..5371
                     /vntifkey="87"
                     /label=Myc
     CDS             4307..5332
                     /vntifkey="4"
                     /label=MALT1\DD-Ig2
BASE COUNT     1545 a      1655 c      1659 g      1624 t 
ORIGIN
        1 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 
       61 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
      121 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
      181 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
      241 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
      301 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
      361 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
      421 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
      481 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
      541 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 
      601 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
      661 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
      721 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
      781 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
      841 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 
      901 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 
      961 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 
     1021 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     1081 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     1141 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 
     1201 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 
     1261 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 
     1321 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 
     1381 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 
     1441 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     1501 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
     1561 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
     1621 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
     1681 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
     1741 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca 
     1801 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
     1861 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
     1921 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
     1981 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 
     2041 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
     2101 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
     2161 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
     2221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
     2281 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
     2341 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
     2401 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
     2461 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
     2521 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
     2581 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
     2641 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 
     2701 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
     2761 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
     2821 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
     2881 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
     2941 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 
     3001 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
     3061 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
     3121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
     3181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
     3241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
     3301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga 
     3361 tcgggagatc tcccgatccc ctatggtcga ctctcagtac aatctgctct gatgccgcat 
     3421 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca 
     3481 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg 
     3541 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat 
     3601 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt 
     3661 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 
     3721 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 
     3781 gtcaatgggt ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 
     3841 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 
     3901 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 
     3961 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 
     4021 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 
     4081 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 
     4141 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt 
     4201 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagcgtttaa 
     4261 acttaagctt accatggatt acaaggacga tgacgataag gtaccaatgg cgctgttggg 
     4321 ggacccgcta caggccctgc cgccctcggc cgcccccacg gggccgctgc tcgcccctcc 
     4381 ggccggcgcg accctcaacc gcctgcggga gccgctgctg cggaggctca gcgagctcct 
     4441 ggatcaggcg cccgagggcc ggggctggag gagactggcg gagctggcgg ggagtcgcgg 
     4501 gcgcctccgc ctcagttgcc tagacctgga gcagtgttct cttaaggtac tggagcctga 
     4561 aggaagcccc agcctgtgtc tgctgaagtt aatgggtgaa aaaggttgca cagtcacaga 
     4621 attgagtgat ttcctgcagg ctatggaaca cactgaagtt cttcagcttc tcagcccccc 
     4681 aggaataaag attactgtaa acccagagtc aaaggcagtc ttggctggac agtttgtgaa 
     4741 actgtgttgc cgggcaactg gacatccttt tgttcaatat cagtggttca aaatgaataa 
     4801 agagattcca aatggaaata catcagagct tatttttaat gcagtgcatg taaaagatgc 
     4861 aggcttttat gtctgtcgag ttaataacaa tttcaccttt gaattcagcc agtggtcaca 
     4921 gctggatgtt tgcgacatcc cagagagctt ccagagaagt gttgatggcg tctctgaatc 
     4981 caagttgcaa atctgtgttg aaccaacttc ccaaaagctg atgccaggca gcacattggt 
     5041 tttacagtgt gttgctgttg gaagccctat tcctcactac cagtggttca aaaatgaatt 
     5101 accattaaca catgagacca aaaagctata catggtgcct tatgtggatt tggaacacca 
     5161 aggaacctac tggtgtcatg tatataatga tcgagacagt caagatagca agaaggtaga 
     5221 aatcatcata ggaagaacag atgaggcagt ggagtgcact gaagatgaat taaataatct 
     5281 tggtcatcct gataataaag agcaaacaac tgaccagcct ttggcgaagg acgggccctt 
     5341 cgaacaaaaa ctcatctcag aagaggatct gaatatgcat accggtcatc atcaccatca 
     5401 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt 
     5461 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 
     5521 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 
     5581 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 
     5641 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc 
     5701 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 
     5761 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 
     5821 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt 
     5881 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 
     5941 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 
     6001 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 
     6061 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 
     6121 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 
     6181 ccaggcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 
     6241 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     6301 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     6361 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 
     6421 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 
     6481 ccc 
//
