ID   pUC18     standard; circular DNA; UNA; 2686 BP.
DE   LMBP 7874.
CC   Accession number: LMBP 7874
CC   (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium)
CC   Last modified: 12-OCT-2012
CC
CC   VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
CC   VNTAUTHORTEL|0032-9-33-13-843|
CC   VNTAUTHOREML|bccm.genecorner@UGent.be|
CC   VNTAUTHORWWW|www.genecorner.ugent.be|
CC   VNTAUTHORAD1|Ghent University|
CC   VNTAUTHORAD2|Technologiepark 927|
CC   VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
CC
CC   VNTREPLTYPE|Plasmid
FH   Key             Location/Qualifiers
FH
FT   CDS             complement(236..469)
FT                   /vntifkey="4"
FT                   /label=lacZ\fragment
FT   RBS             complement(470..507)
FT                   /vntifkey="32"
FT                   /label=5'\UTR\of\lacZ,\incl.\RBS
FT   promoter        complement(508..591)
FT                   /vntifkey="30"
FT                   /label=lac\promoter
FT                   /note="E. coli lac operon promoter"
FT   rep_origin      complement(683..1085)
FT                   /vntifkey="33"
FT                   /label=pMB1\ori
FT   CDS             complement(1626..2486)
FT                   /vntifkey="4"
FT                   /label=ampicillin\resistance
SQ   Sequence 2686 BP; 666 A; 677 C; 684 G; 659 t;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca        60
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg       120
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc       180
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc       240
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat       300
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt       360
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg       420
     actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct       480
     gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt       540
     aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc       600
     gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg       660
     agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg       720
     gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca       780
     gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac       840
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac       900
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg       960
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac      1020
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat      1080
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag      1140
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac      1200
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt      1260
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt      1320
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc      1380
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga      1440
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac      1500
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc      1560
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct      1620
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca      1680
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct      1740
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca      1800
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc      1860
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg      1920
     cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct      1980
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa      2040
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta      2100
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc      2160
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg      2220
     agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa      2280
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg      2340
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc      2400
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg      2460
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat      2520
     cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata      2580
     ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc      2640
     atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc                     2686
//