LOCUS       pCD-F-bio-Card11-L232LI        8916 bp    DNA     circular     30-NOV-2015
DEFINITION  LMBP 7889.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|751902764|
COMMENT     VNTNAME|pCD-F-bio-Card11-L232LI|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCD-F-bio-Card11-L232LI" 0 8916 
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COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 4869 4881 1 8916 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT            (CStringList) 1 1 1 1 "2242..2567") 
COMMENT        #1=(CFSignal (CObList) "f1" 33 0 0 4782 5195 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1764..2177") 
COMMENT        #2=(CFSignal (CObList) "BGH polyA" 25 0 0 4505 4719 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1487..1701") 
COMMENT        #3=(CFSignal (CObList) "flag epitope" 21 0 0 920 946 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "920..946") 
COMMENT        #4=(CFSignal (CObList) "AmpR" 22 0 1 7920 8781 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4902..5763)") 
COMMENT        #5=(CFSignal (CObList) "ColE1" 33 0 0 7102 7775 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4084..4757") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA" 25 0 0 6431 6670 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "NeoR" 86 0 0 5621 6415 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "T7" 30 0 0 863 882 0 (CStringList) (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "mCard11 mutant" 4 0 0 1013 4480 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "L232LI" 62 0 0 1709 1711 0 (CStringList) 
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COMMENT                          "DNA 'pCD-F-bio-Card11-L232LI'" 1) 
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COMMENT                          "Currently local object. Original author: Peter Marynen" 
COMMENT                          1) 
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COMMENT                          "Created: 11/30/15 01:52" 1) 
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COMMENT                          "Last Modified: 11/30/15 01:52" 1) 
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COMMENT                          "length: 8916 bp" 1) 
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FEATURES             Location/Qualifiers
     promoter        5260..5585
                     /vntifkey="29"
                     /label=SV40
     rep_origin      4782..5195
                     /vntifkey="33"
                     /label=f1
     polyA_signal    4505..4719
                     /vntifkey="25"
                     /label=BGH\polyA
     misc_feature    920..946
                     /vntifkey="21"
                     /label=flag\epitope
     misc_marker     complement(7920..8781)
                     /vntifkey="22"
                     /label=AmpR
     rep_origin      7102..7775
                     /vntifkey="33"
                     /label=ColE1
     polyA_signal    6431..6670
                     /vntifkey="25"
                     /label=SV40\polyA
     misc_recomb     5621..6415
                     /vntifkey="86"
                     /label=NeoR
     promoter        863..882
                     /vntifkey="30"
                     /label=T7
     promoter        209..863
                     /vntifkey="29"
                     /label=pCMV
     misc_feature    953..997
                     /vntifkey="21"
                     /label=bio
                     /note="synthetic biotinylation sequence"
     CDS             1013..4480
                     /vntifkey="4"
                     /label=mCard11\mutant
     mutation        1709..1711
                     /vntifkey="62"
                     /label=L232LI
                     /note="insertion of isoleucin codon"
BASE COUNT     2173 a      2385 c      2383 g      1975 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac caggcctgaa 
      961 cgacatcttc gaggcccaga agatcgagtg gcacgaggag gatcctgcca ccatgccagg 
     1021 aggagggcca gctatggatg actacatgga gacgctgaag gatgaagagg aggccctatg 
     1081 ggataacgtg gaatgcaacc ggcacatgct gagccgttac atcaaccccg ccaagctcac 
     1141 cccctacctg cgccagtgca aggtcatcga tgagcaagat gaagacgagg tgctcaatgc 
     1201 gcccatgctg ccgtccaaga tcaaccgtgc aggccgattg ttggacattc ttcacaccaa 
     1261 gggacaaagg ggctatgtgg tcttcctgga gagcctggag ttttactacc cagaacttta 
     1321 caaactggtg actggaaagg aacccacccg gagattctcc accattgtgg tggaggaagg 
     1381 ccatgagggc ctcacacact tcctgatgaa cgaggtcatc aaactgcagc agcaagtgaa 
     1441 agccaaggac cttcagcgct gtgagctgct ggccaagtcc cggcaactgg aggatgagaa 
     1501 gaagcagctg agcctgatac gggtggagct gctgaccttc caggagcgat actacaagat 
     1561 gaaggaggag cgggacagct acaatgacga gctcgtcaag gtcaaggacg acaactacaa 
     1621 cttagccatg cgctacgccc agctcagtga ggagaaaaac atggcggtga tgaggagccg 
     1681 cgacctccaa ctcgagatcg accagctcat taaacaccga ctgaacaaga tggaggagga 
     1741 atgcaagctg gagagaaatc agtccctcaa gctcaagaat gacatcgaga accggcccag 
     1801 gaaggagcag gtcctggagc tggagcggga gaatgagatg ctgaagacga aaattcagga 
     1861 gctgcagtcc atcatccagg ctggcaagcg cagcctccct gactcagaca aggccatctt 
     1921 ggacatcctg gaacatgacc ggaaggaggc gctagaggac cggcaggaac tggtcaacaa 
     1981 aatttacaac ctacaagagg aagtccgcca ggcggaggag ctgcgggata agtacctgga 
     2041 ggagaaggaa gacctggaac tcaagtgttc aaccctgggg aaggactgtg aaatgtacaa 
     2101 gcaccgcatg aacacagtta tgctgcagct ggaggaggtg gagcgggagc gggaccaggc 
     2161 cttccactcc cgagatgagg cacagacaca gtactcacag tgcttaatcg agaaggacaa 
     2221 gtaccggaag cagatccggg agctggagga gaagaacgat gagatgcgta ttgagatggt 
     2281 gaggagggag gcctgtattg tcaacctgga aagcaagctc cggcgcctgt ccaaggacaa 
     2341 cggcagcctc gaccagagtc tgcctagaca ccttccagcc accatcatct cacagaacct 
     2401 tggagacacc agccccagga ccaatggcca ggaagctgat gattcttcaa cctcagaaga 
     2461 gtctcccgaa gacagcaagt actttctgcc ttaccaccca ccccggcgcc ggatgaacct 
     2521 aaagggcatc cagctgcaga gagccaaatc ccccatcagc atgaagcaag catctgagtt 
     2581 tcaagtcaag gggcacgaag aggatttcac agacggcagc cccagttcct cccgctcgct 
     2641 gcctgtcacc agctctttct ccaagatgca accccatcgg agccgcagca gcatcatgtc 
     2701 aatcacggca gagcccccgg gaaatgactc catagtcaga cgctgtaagg aagatgcgcc 
     2761 acaccggagc acggtggaag aagacaacga tagctgtggg tttgatgcct tagaccttga 
     2821 cgatgaaaat cacgaacgtt attcctttgg acctccctcc atccactcct cctcctcttc 
     2881 acaccagtca gagggactgg atgcctacga cctggagcag gtcaacctca tgttacgaaa 
     2941 gttctctttg gaaaggccct tccggccatc ggtcacatct gggggtcacg tgcggggcac 
     3001 cgggcccttg gtccagcaca caactctgaa tggcgatggg ctcatcacgc agctcaccct 
     3061 tctgggcggc aatgcacgcg ggagcttcat tcactctgtc aagccaggct cactggctga 
     3121 gagggccgga ctgcgtgagg gccaccaact cctgctgctg gaaggttgca tccgaggcga 
     3181 aaggcagagc gttccactgg atgcgtgcac aaaagaagag gcccgttgga ccatccagag 
     3241 gtgcagtggc ctcatcactc tgcattacaa ggtcaaccat gaaggatacc ggaagctgct 
     3301 gaaggagatg gaggatggtc tgatcacatc aggggactcg ttctatatcc gcctgaacct 
     3361 gaacatctcc agccagctgg atgcctgctc catgtccctc aagtgtgacg acgtggtgca 
     3421 tgtcctagac accatgtacc aggacaggca cgagtggctg tgtgcacgag tcgacccctt 
     3481 cactgaccaa gacctggaca cgggcaccat ccccagctac agccgggctc aacagcttct 
     3541 cctggtgaag ctccagcggt tggttcacag aggcaaccgg gaagaggcag acagcgctca 
     3601 ccacaccctg cgcagcctcc ggaacaccct gcagcccgaa gagatgcttt cgacgagcga 
     3661 cccccgagtc agcccccgcc tctccagagc gagtttcttc tttggccagc tcctgcagtt 
     3721 tgtcagccgg tcagaaaaca agtacaaaag aatgaacagc aatgagcgcg tgagaatcat 
     3781 ctctgggagt cccctgggga gcctctcccg gtcctcgctg gatgccacca aactcctgac 
     3841 cgagaagcat gaagaactgg atcctgagaa tgagctcagc cggaacctca ccctgatccc 
     3901 ttacagcctg gtgcgcgctt tccactgtga gcgccgcagg cctgtgctct tcacgcccac 
     3961 catgctggcc aagacattgg tgcagaagct gctcaactca gggggtgcca tggagttcac 
     4021 catctgcaag tcagatattg tcacaagaga tgagttcctc cgaaagcaga agacagagac 
     4081 catcatctac tcccgggaaa agaaccccaa cacctttgaa tgcatcgtcc ctgccaacat 
     4141 tgaggctgtg gcagccaaga acaaacactg cctgctggag gctgggatcg gctgtgtgcg 
     4201 cgacctgatc aagtgcaagg tgtaccccat agtgctgctc atccgggtga gcgagaagaa 
     4261 catcaaacgg ttcaggaagc tgctgccgcg gccagagacg gaagaggaat tcctgcgagt 
     4321 gtgcaggctc aaagagaagg agctggaggc gctgccctgc ctctacgcca ccgtggaagc 
     4381 tgagatgtgg agcagcgtgg aggagctgct gcgagtcctc aaagacaaga ttgtagagga 
     4441 gcagcgcaag accatctggg tggacgagga ccagctgtga gggcccgttt aaacccgctg 
     4501 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 
     4561 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 
     4621 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 
     4681 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 
     4741 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 
     4801 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 
     4861 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 
     4921 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga 
     4981 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 
     5041 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 
     5101 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc 
     5161 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 
     5221 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca 
     5281 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 
     5341 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 
     5401 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 
     5461 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 
     5521 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 
     5581 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 
     5641 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 
     5701 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 
     5761 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 
     5821 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 
     5881 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 
     5941 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 
     6001 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 
     6061 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 
     6121 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 
     6181 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 
     6241 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 
     6301 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 
     6361 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 
     6421 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 
     6481 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 
     6541 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 
     6601 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     6661 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 
     6721 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 
     6781 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 
     6841 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 
     6901 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 
     6961 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
     7021 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
     7081 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     7141 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     7201 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     7261 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     7321 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 
     7381 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     7441 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     7501 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     7561 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 
     7621 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     7681 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     7741 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     7801 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     7861 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     7921 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     7981 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     8041 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     8101 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     8161 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     8221 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     8281 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     8341 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     8401 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     8461 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     8521 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     8581 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     8641 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     8701 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     8761 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     8821 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     8881 ccgcgcacat ttccccgaaa agtgccacct gacgtc 
//
