LOCUS       pDG1i-tdTomato-V        9808 bp    DNA     circular     23-JUN-2023
DEFINITION  LMBP 7942.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7942
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-JUN-2023
            NGS data implemented
FEATURES             Location/Qualifiers
     misc_feature    3217..3258
                     /label=V5\epitope
     promoter        3677..3742
                     /label=synthetic\EM7\promoter
     CDS             268..927
                     /label=chloramphenicol\resistance
     CDS             1269..1574
                     /label=ccdB
     CDS             3743..4141
                     /label=blasticidin\resistance
     misc_recomb     1615..1739
                     /label=attR2
     misc_recomb     complement(35..159)
                     /label=attR1
     promoter        3291..3572
                     /label=SV40\early\promoter
     rep_origin      3529..3604
                     /label=SV40\ori
     terminator      4533..4607
                     /label=SV40\polyA\early
     promoter        7789..8117
                     /label=RSV/HIV-1\5'\LTR
     rep_origin      5036..5491
                     /label=f1\ori
     CDS             5622..6482
                     /label=ampicillin\resistance
     rep_origin      6653..7244
                     /label=pMB1\ori
     promoter        7517..7600
                     /label=lac\promoter
     promoter        7664..7680
                     /label=T3\promoter
     terminator      complement(4533..4607)
                     /label=SV40\polyA\late
     rep_origin      4743..4818
                     /label=SV40\ori
     promoter        complement(4852..4868)
                     /label=T7\promoter
     misc_feature    8228..8272
                     /label=HIV-1\psi\packaging\signal
     misc_feature    8782..9015
                     /label=HIV-1\Rev\response\element
     terminator      4227..4461
                     /label=RSV/HIV-1\dU3/3'\LTR
     misc_feature    4211..4226
                     /label=HIV-1\cPPT
     promoter        9532..9800
                     /label=hCMVTRE\promoter
     CDS             1771..3198
                     /label=tdTomato
     promoter        167..219
                     /label=lacUV5\promoter
BASE COUNT     2574 a      2372 c      2520 g      2342 t 
ORIGIN
        1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg 
       61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac 
      121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca 
      181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg 
      241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt 
      301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt 
      361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat 
      421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg 
      481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt 
      541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac 
      601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg 
      661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg 
      721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc 
      781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt 
      841 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac 
      901 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa 
      961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc 
     1021 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg 
     1081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac 
     1141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg 
     1201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg 
     1261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg 
     1321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca 
     1381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg 
     1441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg 
     1501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt 
     1561 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt 
     1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt 
     1681 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt 
     1741 gatatccagc acagtggcgg ccgctcgagt atggtgagca agggcgagga ggtcatcaaa 
     1801 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc 
     1861 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 
     1921 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc 
     1981 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag 
     2041 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag 
     2101 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc 
     2161 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc 
     2221 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac 
     2281 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg 
     2341 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc 
     2401 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc 
     2461 ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc 
     2521 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc 
     2581 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag 
     2641 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac 
     2701 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc 
     2761 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg 
     2821 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc 
     2881 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc 
     2941 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg 
     3001 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg 
     3061 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac 
     3121 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc 
     3181 atggacgagc tgtacaagga gggcccgcgg ttcgaaggta agcctatccc taaccctctc 
     3241 ctcggtctcg attctacgcg taccggttag taatgagttt ggaattaatt ctgtggaatg 
     3301 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 
     3361 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 
     3421 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 
     3481 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 
     3541 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 
     3601 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg 
     3661 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata 
     3721 cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat 
     3781 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag 
     3841 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg 
     3901 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct 
     3961 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg 
     4021 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg 
     4081 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta 
     4141 agcacaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta 
     4201 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag 
     4261 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct 
     4321 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag 
     4381 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt 
     4441 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac 
     4501 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 
     4561 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 
     4621 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 
     4681 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 
     4741 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 
     4801 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag 
     4861 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 
     4921 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 
     4981 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg 
     5041 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 
     5101 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 
     5161 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 
     5221 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 
     5281 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 
     5341 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 
     5401 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 
     5461 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg 
     5521 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 
     5581 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 
     5641 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 
     5701 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 
     5761 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 
     5821 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 
     5881 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 
     5941 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 
     6001 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 
     6061 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 
     6121 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 
     6181 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 
     6241 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 
     6301 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 
     6361 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 
     6421 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 
     6481 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 
     6541 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 
     6601 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 
     6661 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 
     6721 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 
     6781 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 
     6841 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 
     6901 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 
     6961 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 
     7021 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 
     7081 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 
     7141 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 
     7201 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 
     7261 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 
     7321 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 
     7381 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 
     7441 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 
     7501 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc 
     7561 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat 
     7621 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa 
     7681 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg 
     7741 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg 
     7801 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct 
     7861 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct 
     7921 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 
     7981 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 
     8041 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 
     8101 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag 
     8161 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg 
     8221 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc 
     8281 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg 
     8341 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa 
     8401 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga 
     8461 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta 
     8521 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac 
     8581 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt 
     8641 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt 
     8701 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag 
     8761 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag 
     8821 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat 
     8881 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact 
     8941 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa 
     9001 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt 
     9061 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg 
     9121 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga 
     9181 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag 
     9241 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat 
     9301 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt 
     9361 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga 
     9421 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt 
     9481 agtgaacgga tctcgacggt atcgatggtc cgaggttcta gacgagttta ctccctatca 
     9541 gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt 
     9601 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg 
     9661 tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt 
     9721 gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg 
     9781 tttagtgaac cgtcagatcg caccggtc 
//
