ID pDG2i-hRIP4-K51R-FLAG-puro standard; circular DNA; UNA; 10606 BP. DT 12-JAN-2011 DE LMBP 7948 CC Accession number: LMBP 7948 CC (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium) CC Last modified: 2-MAR-2020 CC CC This file is created by Vector NTI CC http://www.invitrogen.com/ CC ORIGDB|GenBank CC VNTDBDATE|-13236086| CC LSOWNER| CC VNTAUTHORNAME|Demo User| CC VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTOAUTHORTEL|0032-9-33-13-843| CC VNTOAUTHOREML|bccm.genecorner@UGent.be| CC VNTOAUTHORWWW|www.genecorner.ugent.be| CC VNTOAUTHORAD1|Ghent University| CC VNTOAUTHORAD2|Technologiepark 927| CC VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS <1..2352 FT /vntifkey="4" FT /label=hRIPK4\mutant FT misc_marker 2437..2460 FT /vntifkey="22" FT /label=FLAG\epitope FT promoter 2496..2777 FT /vntifkey="29" FT /label=SV40\early\promoter FT CDS 2887..3594 FT /vntifkey="4" FT /label=rtTA3 FT misc_feature <3595..3628 FT /vntifkey="21" FT /label=loxP FT CDS <4226..4828 FT /vntifkey="4" FT /label=puromycin\resistance FT terminator 4919..5153 FT /vntifkey="43" FT /label=RSV/HIV-1\dU3/3'\LTR FT terminator 5225..5299 FT /vntifkey="43" FT /label=SV40\polyA\early FT rep_origin 5728..6183 FT /vntifkey="33" FT /label=f1\ori FT CDS 6314..7174 FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 7715..8117 FT /vntifkey="33" FT /label=pMB1\ori FT promoter 8481..8809 FT /vntifkey="29" FT /label=RSV/HIV-1\5'\LTR FT misc_feature 8920..8964 FT /vntifkey="21" FT /label=HIV-1\psi\packaging\signal FT misc_feature 9474..9707 FT /vntifkey="21" FT /label=HIV-1\Rev\response\element FT attenuator complement(2369..2393) FT /vntifkey="0" FT /label=attB2 FT RBS 3651..4222 FT /vntifkey="32" FT /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES) FT misc_feature 4903..4918 FT /vntifkey="21" FT /label=HIV-1\cPPT FT terminator complement(5225..5299) FT /vntifkey="43" FT /label=SV40\polyA\late FT rep_origin 5435..5510 FT /vntifkey="33" FT /label=SV40\ori FT promoter complement(5544..5560) FT /vntifkey="30" FT /label=T7\promoter FT promoter 8209..8292 FT /vntifkey="30" FT /label=lac\promoter FT promoter 10224..10492 FT /vntifkey="29" FT /label=hCMVTRE\promoter FT attenuator 10535..10559 FT /vntifkey="0" FT /label=attB1 FT promoter 8356..8372 FT /vntifkey="30" FT /label=T3\promoter FT mutation 151..153 FT /vntifkey="62" FT /label=K51R FT rep_origin 2734..2809 FT /vntifkey="33" FT /label=SV40\ori SQ Sequence 10606 BP; 2585 A; 2798 C; 2890 G; 2333 t; atggagggcg acggcgggac cccatgggcc ctggcgctgc tgcgcacctt cgacgcgggc 60 gagttcacgg gctgggagaa ggtgggctcg ggcggcttcg ggcaggtgta caaggtgcgc 120 catgtccact ggaagacctg gctcgcgatc aggtgctcgc ccagcctgca cgtcgacgac 180 agggagcgca tggagctttt ggaagaagcc aagaagatgg agatggccaa gtttcgctac 240 atcctgcctg tgtatggcat ctgccgcgaa cctgtcggcc tggtcatgga gtacatggag 300 acgggctccc tggaaaagct gctggcttcg gagccattgc catgggatct ccggttccga 360 atcatccacg agacggcggt gggcatgaac ttcctgcact gcatggcccc gccactcctg 420 cacctggacc tcaagcccgc gaacatcctg ctggatgccc actaccacgt caagatttct 480 gattttggtc tggccaagtg caacgggctg tcccactcgc atgacctcag catggatggc 540 ctgtttggca caatcgccta cctccctcca gagcgcatca gggagaagag ccggctcttc 600 gacaccaagc acgatgtata cagctttgcg atcgtcatct ggggcgtgct cacacagaag 660 aagccgtttg cagatgagaa gaacatcctg cacatcatgg tgaaggtggt gaagggccac 720 cgccccgagc tgccgcccgt gtgcagagcc cggccgcgcg cctgcagcca cctgatacgc 780 ctcatgcagc ggtgctggca gggggatccg cgagttaggc ccaccttcca agaaattact 840 tctgaaaccg aggacctgtg tgaaaagcct gatgacgaag tgaaagaaac tgctcatgat 900 ctggacgtga aaagcccccc ggagcccagg agcgaggtgg tgcctgcgag gctcaagcgg 960 gcctctgccc ccaccttcga taacgactac agcctctccg agcttctctc acagctggac 1020 tctggagttt cccaggctgt cgagggcccc gaggagctca gccgcagctc ctctgagtcc 1080 aagctgccat cgtccggcag tgggaagagg ctctcggggg tgtcctcggt ggactccgcc 1140 ttctcttcca gaggatcact gtcgctgtcc tttgagcggg aaccttcaac cagcgatctg 1200 ggtaccacag acgtccagaa gaagaagctt gtggatgcca tcgtgtccgg ggacaccagc 1260 aaactgatga agatcctgca gccgcaggac gtggacctgg cactggacag cggtgccagc 1320 ctgctgcacc tggcggtgga ggccgggcaa gaggagtgcg ccaagtggct gctgctcaac 1380 aatgccaacc ccaacctgag caaccgtagg ggctccaccc cgttgcacat ggccgtggag 1440 aggagggtgc ggggtgtcgt ggagctcctg ctggcacgga agatcagtgt caacgccaag 1500 gatgaggacc agtggacagc cctccacttt gcagcccaga acggggatga gtctagcaca 1560 cggctgctgt tggagaagaa cgcctcggtc aacgaggtgg actttgaggg ccggacgccc 1620 atgcacgtgg cctgccagca cgggcaggag aatatcgtgc gcatcctgct gcgccgaggc 1680 gtggacgtga gcctgcaggg caaggatgcc tggctgccac tgcactacgc tgcctggcag 1740 ggccacctgc ccatcgtcaa gctgctggcc aagcagccgg gggtgagtgt gaacgcccag 1800 acgctggatg ggaggacgcc attgcacctg gccgcacagc gcgggcacta ccgcgtggcc 1860 cgcatcctca tcgacctgtg ctccgacgtc aacgtctgca gcctgctggc acagacaccc 1920 ctgcacgtgg ccgcggagac ggggcacacg agcactgcca ggctgctcct gcatcggggc 1980 gctggcaagg aggccgtgac ctcagacggc tacaccgctc tgcacctggc tgcccgcaac 2040 ggacacctgg ccactgtcaa gctgcttgtc gaggagaagg ccgatgtgct ggcccgggga 2100 cccctgaacc agacggcgct gcacctggct gccgcccacg ggcactcgga ggtggtggag 2160 gagttggtca gcgccgatgt cattgacctg ttcgacgagc aggggctcag cgcgctgcac 2220 ctggccgccc agggccggca cgcacagacg gtggagactc tgctcaggca tggggcccac 2280 atcaacctgc agagcctcaa gttccagggc ggccatggcc ccgccgccac actcctgcgg 2340 cgaagcaaga cccactcgag atatctagac ccagctttct tgtacaaagt ggttgatatc 2400 cagcacagtg gcggccgctc gagtctagag ggcccggact acaaggatga cgatgacaaa 2460 acgcgtaccg gttagtaatg agtttggaat taattctgtg gaatgtgtgt cagttagggt 2520 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2580 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2640 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 2700 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 2760 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 2820 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcaac 2880 gtgaccatgt ctaggctgga caagagcaaa gtcataaacg gagctctgga attactcaat 2940 ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag 3000 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag 3060 atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt 3120 ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa 3180 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg 3240 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc 3300 cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga 3360 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac 3420 cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag 3480 aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac 3540 atgctcccag ccgatgccct tgacgatttt gaccttgaca tgctccccgg gtaaataact 3600 tcgtatagta tacattatac gaagttatgg atccgcggcc gcaaattccg cccctctccc 3660 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc 3720 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc 3780 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt 3840 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct 3900 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa 3960 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt 4020 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag 4080 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt 4140 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt 4200 ttcctttgaa aaacacgata ataccatggc caccgagtac aagcccacgg tgcgcctcgc 4260 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 4320 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga 4380 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 4440 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 4500 cggctcgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 4560 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 4620 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 4680 cgagcgcgct ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 4740 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 4800 gtgcatgacc cgcaagcccg gtgcctgagc taagcacaat tcgagctcgg tacctttaag 4860 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact 4920 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct 4980 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 5040 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 5100 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag 5160 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga 5220 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 5280 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 5340 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact 5400 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 5460 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 5520 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg 5580 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 5640 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 5700 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg 5760 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 5820 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 5880 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 5940 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 6000 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 6060 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 6120 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa 6180 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 6240 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 6300 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 6360 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 6420 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 6480 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 6540 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 6600 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 6660 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 6720 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 6780 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 6840 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 6900 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 6960 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 7020 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 7080 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 7140 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 7200 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 7260 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 7320 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 7380 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 7440 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 7500 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 7560 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 7620 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 7680 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 7740 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 7800 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 7860 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 7920 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 7980 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 8040 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 8100 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 8160 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 8220 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg 8280 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga 8340 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta 8400 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat 8460 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat 8520 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 8580 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 8640 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 8700 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 8760 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 8820 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 8880 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 8940 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 9000 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 9060 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 9120 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 9180 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 9240 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 9300 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac 9360 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 9420 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct 9480 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg 9540 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg 9600 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag 9660 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt 9720 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 9780 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat 9840 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 9900 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 9960 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 10020 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 10080 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 10140 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatgg 10200 tccgaggttc tagacgagtt tactccctat cagtgataga gaacgatgtc gagtttactc 10260 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg 10320 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata 10380 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt 10440 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcaccggtc 10500 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctc 10560 tttaaaggaa ccaattcagt cgactggatc cggtaccgaa ttcacc 10606 //