LOCUS       pCAXL-Mx1K49A           6749 bp    DNA     circular     13-JUL-2015
DEFINITION  LMBP 7971.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|739363243|
COMMENT     VNTNAME|pCAXL-Mx1K49A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCAXL-Mx1K49A" 0 6749 
COMMENT       (CDBMol 0 0 2 3740 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 5 4294967295 50 0 
COMMENT        1 0 (CHomObj 0 0 0 3 75) (CWordArray 4 29) (CWordArray) 
COMMENT        (CStringList "AatII" "Acc65I" "AccI" "AgeI" "AluI" "ApaI" "AvaI" "AvaII" 
COMMENT         "BamHI" "BanI" "BanII" "BclI" "BglI" "BglII" "Bsp1286I" "BssHII" 
COMMENT         "BstEII" "BstXI" "Bsu36I" "ClaI" "DdeI" "DpnI" "DraI" "EcoICRI" "EcoRI" 
COMMENT         "EcoRV" "FokI" "HaeII" "HaeIII" "HincII" "HindIII" "HinfI" "HpaI" 
COMMENT         "HpaII" "KpnI" "MboI" "MboII" "MluI" "MscI" "MspA1I" "NaeI" "NarI" 
COMMENT         "NciI" "NcoI" "NdeI" "NgoMIV" "NheI" "NotI" "NruI" "NsiI" "PacI" "PmeI" 
COMMENT         "PstI" "PvuI" "PvuII" "RsaI" "SacI" "SacII" "SalI" "ScaI" "SfiI" "SmaI" 
COMMENT         "SnaBI" "SpeI" "SphI" "SspI" "StuI" "StyI" "Tth111I" "XbaI" "XhoI" 
COMMENT         "XmaI" "XmnI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 6749 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AatII" "gacgtc" 6 0 3 6254 0 6307 0 6390 0 0 
COMMENT            "") #1=(CRSite (CStringList) "Acc65I" "ggtacc" 2 0 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "AccI" "gtmkac" 3 0 2 2599 0 6112 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AgeI" "accggt" 2 0 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AluI" "agct" 3 0 30 1037 0 1258 0 1346 0 1403 
COMMENT            0 1487 0 1507 0 1874 0 1997 0 2075 0 2339 0 2556 0 2655 0 2870 0 
COMMENT            2896 0 2984 0 3531 0 3578 0 3600 0 3695 0 3924 0 3978 0 3997 0 4236 
COMMENT            0 4462 0 4552 0 4598 0 4855 0 5376 0 5476 0 5539 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "ApaI" "gggccc" 6 0 1 229 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 6 250 0 511 0 631 0 647 0 
COMMENT            907 0 1092 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "AvaII" "ggwcc" 2 0 8 1205 0 1238 0 1712 0 1729 
COMMENT            0 2444 0 2609 0 5325 0 5547 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 3771 0 4112 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "BanI" "ggyrcc" 2 0 6 387 0 608 0 788 0 831 0 
COMMENT            3679 0 5135 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "BanII" "grgcyc" 6 0 10 218 0 229 0 550 0 574 
COMMENT             0 648 0 1190 0 1260 0 1405 0 1999 0 6512 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "BclI" "tgatca" 2 0 1 2252 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 5 3913 0 5307 0 6219 
COMMENT             0 6341 0 6412 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "BglII" "agatct" 2 0 1 3109 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "Bsp1286I" "gdgchc" 6 0 17 57 0 218 0 229 0 
COMMENT             392 0 526 0 550 0 574 0 648 0 1190 0 1260 0 1405 0 1969 0 1999 0 
COMMENT             4612 0 5773 0 5858 0 6512 0 0 "") 
COMMENT        #15=(CRSite (CStringList) "BssHII" "gcgcgc" 2 0 4 14 0 6620 0 6622 0 
COMMENT             6700 0 0 "") 
COMMENT        #16=(CRSite (CStringList) "BstEII" "ggtnacc" 2 0 0 0 "") 
COMMENT        #17=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 3 1646 0 1667 0 
COMMENT             3088 0 0 "") 
COMMENT        #18=(CRSite (CStringList) "Bsu36I" "cctnagg" 3 0 3 1182 0 2287 0 3039 0 
COMMENT             0 "") #19=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
COMMENT        #20=(CRSite (CStringList) "DdeI" "ctnag" 2 0 20 520 0 1182 0 1692 0 1702 
COMMENT             0 1875 0 1885 0 1990 0 2287 0 2437 0 2512 0 2656 0 2710 0 2740 0 
COMMENT             3039 0 3106 0 3920 0 4569 0 4978 0 5144 0 5684 0 0 "") 
COMMENT        #21=(CRSite (CStringList) "DpnI" "gatc" 3 0 27 1411 0 1476 0 1599 0 1756 
COMMENT             0 1921 0 2038 0 2254 0 2568 0 2640 0 2802 0 3111 0 3554 0 3773 0 
COMMENT             4114 0 4862 0 4937 0 4948 0 4956 0 5034 0 5046 0 5151 0 5492 0 5510 
COMMENT             0 5556 0 5814 0 5831 0 5867 0 0 "") 
COMMENT        #22=(CRSite (CStringList) "DraI" "tttaaa" 4 0 6 2273 0 2494 0 3264 0 
COMMENT             5053 0 5072 0 5764 0 0 "") 
COMMENT        #23=(CRSite (CStringList) "EcoICRI" "gagctc" 4 0 3 1258 0 1403 0 1997 0 
COMMENT             0 "") 
COMMENT        #24=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 1086 0 1097 0 3029 0 0 
COMMENT             "") #25=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 0 0 "") 
COMMENT        #26=(CRSite (CStringList) "FokI" "ggatg" 15 19 13 1512 0 1608 1 1803 0 
COMMENT             1848 1 2132 0 2262 0 2811 1 2979 0 3446 1 3811 1 5153 1 5334 1 5621 
COMMENT             1 0 "") 
COMMENT        #27=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 8 162 0 288 0 324 0 678 0 
COMMENT             792 0 835 0 4172 0 4542 0 0 "") 
COMMENT        #28=(CRSite (CStringList) "HaeIII" "ggcc" 3 0 33 134 0 227 0 297 0 378 0 
COMMENT             529 0 626 0 636 0 667 0 862 0 1157 0 1212 0 1287 0 1431 0 1468 0 
COMMENT             1959 0 2332 0 3013 0 3080 0 3901 0 3907 0 3916 0 3959 0 4135 0 4309 
COMMENT             0 4320 0 4338 0 4772 0 5230 0 5310 0 5577 0 6213 0 6406 0 6746 0 0 
COMMENT             "") 
COMMENT        #29=(CRSite (CStringList) "HincII" "gtyrac" 4 0 2 2731 0 6113 0 0 "") 
COMMENT        #30=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 2982 0 3576 0 0 "") 
COMMENT        #31=(CRSite (CStringList) "HinfI" "gantc" 2 0 11 29 0 1113 0 1529 0 2280 
COMMENT             0 2389 0 2406 0 4129 0 4194 0 4269 0 4665 0 5182 0 0 "") 
COMMENT        #32=(CRSite (CStringList) "HpaI" "gttaac" 4 0 0 0 "") 
COMMENT        #33=(CRSite (CStringList) "HpaII" "ccgg" 2 0 25 87 0 145 0 219 0 308 0 
COMMENT             379 0 531 0 582 0 612 0 637 0 670 0 678 0 835 0 981 0 2210 0 2333 0 
COMMENT             3653 0 4501 0 4648 0 4674 0 4864 0 5268 0 5302 0 5369 0 5479 0 5721 
COMMENT             0 0 "") #34=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 0 0 "") 
COMMENT        #35=(CRSite (CStringList) "MboI" "gatc" 1 0 27 1409 0 1474 0 1597 0 1754 
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COMMENT             4112 0 4860 0 4935 0 4946 0 4954 0 5032 0 5044 0 5149 0 5490 0 5508 
COMMENT             0 5554 0 5812 0 5829 0 5865 0 0 "") 
COMMENT        #36=(CRSite (CStringList) "MboII" "gaaga" 14 13 22 1013 1 1437 0 1832 0 
COMMENT             1901 1 1904 1 1932 1 1959 0 2046 0 2115 0 2415 0 2645 0 2678 0 2942 
COMMENT             0 2945 0 3531 1 3562 0 4165 1 4956 0 5027 1 5782 1 5860 1 5969 1 0 
COMMENT             "") #37=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 3018 0 0 "") 
COMMENT        #38=(CRSite (CStringList) "MscI" "tggcca" 4 0 1 3080 0 0 "") 
COMMENT        #39=(CRSite (CStringList) "MspA1I" "cmgckg" 4 0 6 395 0 1874 0 2896 0 
COMMENT             4636 0 4881 0 5822 0 0 "") 
COMMENT        #40=(CRSite (CStringList) "NaeI" "gccggc" 4 0 2 679 0 836 0 0 "") 
COMMENT        #41=(CRSite (CStringList) "NarI" "ggcgcc" 3 0 2 789 0 832 0 0 "") 
COMMENT        #42=(CRSite (CStringList) "NciI" "ccsgg" 3 0 14 87 0 146 0 220 0 308 0 
COMMENT             380 0 531 0 583 0 613 0 638 0 670 0 2334 0 4674 0 5370 0 5721 0 0 
COMMENT             "") 
COMMENT        #43=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 3867 0 6489 0 0 "") 
COMMENT        #44=(CRSite (CStringList) "NdeI" "catatg" 3 0 4 3244 0 3304 0 3311 0 
COMMENT             6363 0 0 "") 
COMMENT        #45=(CRSite (CStringList) "NgoMIV" "gccggc" 2 0 2 677 0 834 0 0 "") 
COMMENT        #46=(CRSite (CStringList) "NheI" "gctagc" 2 0 0 0 "") 
COMMENT        #47=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3011 0 0 "") 
COMMENT        #48=(CRSite (CStringList) "NruI" "tcgcga" 4 0 0 0 "") 
COMMENT        #49=(CRSite (CStringList) "NsiI" "atgcat" 6 0 1 3782 0 0 "") 
COMMENT        #50=(CRSite (CStringList) "PacI" "ttaattaa" 6 0 0 0 "") 
COMMENT        #51=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 0 0 "") 
COMMENT        #52=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 1583 0 2080 0 3571 0 0 
COMMENT             "") #53=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 5557 0 0 "") 
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COMMENT        #55=(CRSite (CStringList) "RsaI" "gtac" 3 0 9 2085 0 2293 0 2507 0 2820 
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COMMENT        #56=(CRSite (CStringList) "SacI" "gagctc" 6 0 3 1260 0 1405 0 1999 0 0 
COMMENT             "") #57=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 396 0 0 "") 
COMMENT        #58=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 6111 0 0 "") 
COMMENT        #59=(CRSite (CStringList) "ScaI" "agtact" 4 0 2 2820 0 5667 0 0 "") 
COMMENT        #60=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 1 3913 0 0 "") 
COMMENT        #61=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #62=(CRSite (CStringList) "SnaBI" "tacgta" 4 0 1 6469 0 0 "") 
COMMENT        #63=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 6128 0 0 "") 
COMMENT        #64=(CRSite (CStringList) "SphI" "gcatgc" 6 0 0 0 "") 
COMMENT        #65=(CRSite (CStringList) "SspI" "aatatt" 4 0 3 2268 0 2789 0 5991 0 0 
COMMENT             "") 
COMMENT        #66=(CRSite (CStringList) "StuI" "aggcct" 4 0 2 1468 0 3959 0 0 "") 
COMMENT        #67=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 4 2671 0 3867 0 3960 0 
COMMENT             6489 0 0 "") 
COMMENT        #68=(CRSite (CStringList) "Tth111I" "gacnnngtc" 5 0 0 0 "") 
COMMENT        #69=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 990 0 0 "") 
COMMENT        #70=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1092 0 0 "") 
COMMENT        #71=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "") 
COMMENT        #72=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 2937 0 5786 0 0 "")) 
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COMMENT        #81=(CFSignal (CObList) "K49A" 62 0 0 1253 1255 0 (CStringList) 
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COMMENT                          "DNA 'pCAXL-Mx1K49A'" 1) 
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COMMENT                          "Currently local object. Original author: Judith Verhelst" 
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COMMENT                          "Created: 07/13/15 10:40" 1) 
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COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 1253  End: 1255" 1) 
COMMENT                                     #143=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "WT: AAG" 1))) "K49A"))) 
COMMENT                           "Mutation (1 total)"))) "Feature Map") 
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COMMENT                     (CObjectList 
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COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28867904 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AatII: 3 sites") 5 1 "GACGTC" 
COMMENT                            "CTGCAG") 
COMMENT                      #146=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28867816 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "Acc65I: 0 sites") 1 5 "GGTACC" 
COMMENT                            "CCATGG") 
COMMENT                      #147=(CRSFolderPar 
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COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 2599 " 1) 
COMMENT                                      #150=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6112 " 1))))) "AccI: 2 sites") 
COMMENT                            2 4 "GTMKAC" "CAKMTG") 
COMMENT                      #151=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28867040 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AgeI: 0 sites") 1 5 "ACCGGT" 
COMMENT                            "TGGCCA") 
COMMENT                      #152=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28866528 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AluI: 30 sites") 2 2 "AGCT" "TCGA") 
COMMENT                      #153=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28866128 (8 0) 1 1 1 0 178) 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 229 " 1))))) "ApaI: 1 site") 5 
COMMENT                            1 "GGGCCC" "CCCGGG") 
COMMENT                      #156=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28865904 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AvaI: 6 sites") 1 5 "CYCGRG" 
COMMENT                            "GRGCYC") 
COMMENT                      #157=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28864832 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "AvaII: 8 sites") 1 4 "GGWCC" 
COMMENT                            "CCWGG") 
COMMENT                      #158=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28864624 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 3771 " 1) 
COMMENT                                      #161=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #8# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 4112 " 1))))) "BamHI: 2 sites") 
COMMENT                            1 5 "GGATCC" "CCTAGG") 
COMMENT                      #162=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28279256 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BanI: 6 sites") 1 5 "GGYRCC" 
COMMENT                            "CCRYGG") 
COMMENT                      #163=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28870496 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BanII: 10 sites") 5 1 "GRGCYC" 
COMMENT                            "CYCGRG") 
COMMENT                      #164=(CRSFolderPar 
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COMMENT                             (CGroupPar (CParagraph 28870408 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 2252 " 1))))) "BclI: 1 site") 
COMMENT                            1 5 "TGATCA" "ACTAGT") 
COMMENT                      #167=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28869424 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BglI: 5 sites") 7 4 "GCCNNNNNGGC" 
COMMENT                            "CGGNNNNNCCG") 
COMMENT                      #168=(CRSFolderPar 
COMMENT                            (CFolderPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 3109 " 1))))) "BglII: 1 site") 
COMMENT                            1 5 "AGATCT" "TCTAGA") 
COMMENT                      #171=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28097616 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "Bsp1286I: 17 sites") 5 1 "GDGCHC" 
COMMENT                            "CHCGDG") 
COMMENT                      #172=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28102848 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BssHII: 4 sites") 1 5 "GCGCGC" 
COMMENT                            "CGCGCG") 
COMMENT                      #173=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28868688 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BstEII: 0 sites") 1 6 "GGTNACC" 
COMMENT                            "CCANTGG") 
COMMENT                      #174=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28109272 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "BstXI: 3 sites") 8 4 
COMMENT                            "CCANNNNNNTGG" "GGTNNNNNNACC") 
COMMENT                      #175=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28093032 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "Bsu36I: 3 sites") 2 5 "CCTNAGG" 
COMMENT                            "GGANTCC") 
COMMENT                      #176=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28059536 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" 
COMMENT                            "TAGCTA") 
COMMENT                      #177=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28060000 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "DdeI: 20 sites") 1 4 "CTNAG" 
COMMENT                            "GANTC") 
COMMENT                      #178=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28125888 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "DpnI: 27 sites") 2 2 "GATC" "CTAG") 
COMMENT                      #179=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28125656 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "DraI: 6 sites") 3 3 "TTTAAA" 
COMMENT                            "AAATTT") 
COMMENT                      #180=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28052552 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EcoICRI: 3 sites") 3 3 "GAGCTC" 
COMMENT                            "CTCGAG") 
COMMENT                      #181=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28060824 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EcoRI: 3 sites") 1 5 "GAATTC" 
COMMENT                            "CTTAAG") 
COMMENT                      #182=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28047752 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "EcoRV: 0 sites") 3 3 "GATATC" 
COMMENT                            "CTATAG") 
COMMENT                      #183=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28104304 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "FokI: 13 sites") 14 18 
COMMENT                            "GGATGNNNNNNNNNNNNNN" "CCTACNNNNNNNNNNNNNN") 
COMMENT                      #184=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28118232 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "HaeII: 8 sites") 5 1 "RGCGCY" 
COMMENT                            "YCGCGR") 
COMMENT                      #185=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28100488 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "HaeIII: 33 sites") 2 2 "GGCC" 
COMMENT                            "CCGG") 
COMMENT                      #186=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28100280 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #187=(CGroupPar 
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COMMENT                                     (CObjectList 
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COMMENT                                            " N1: 2731 " 1) 
COMMENT                                      #189=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #29# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6113 " 1))))) 
COMMENT                             "HincII: 2 sites") 3 3 "GTYRAC" "CARYTG") 
COMMENT                      #190=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28125192 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #191=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #30# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #192=(CLinePar 
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COMMENT                                            " N1: 2982 " 1) 
COMMENT                                      #193=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #30# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 3576 " 1))))) 
COMMENT                             "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") 
COMMENT                      #194=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 27939640 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "HinfI: 11 sites") 1 4 "GANTC" 
COMMENT                            "CTNAG") 
COMMENT                      #195=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28126944 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "HpaI: 0 sites") 3 3 "GTTAAC" 
COMMENT                            "CAATTG") 
COMMENT                      #196=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28126712 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "HpaII: 25 sites") 1 3 "CCGG" 
COMMENT                            "GGCC") 
COMMENT                      #197=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28119008 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "KpnI: 0 sites") 5 1 "GGTACC" 
COMMENT                            "CCATGG") 
COMMENT                      #198=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28061056 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "MboI: 27 sites") 1 5 "NGATCN" 
COMMENT                            "NCTAGN") 
COMMENT                      #199=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28030256 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "MboII: 22 sites") 13 12 
COMMENT                            "GAAGANNNNNNNNN" "CTTCTNNNNNNNNN") 
COMMENT                      #200=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28124288 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #201=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #37# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #202=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #37# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3018 " 1))))) "MluI: 1 site") 
COMMENT                            1 5 "ACGCGT" "TGCGCA") 
COMMENT                      #203=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28059768 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #204=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #38# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #205=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #38# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3080 " 1))))) "MscI: 1 site") 
COMMENT                            3 3 "TGGCCA" "ACCGGT") 
COMMENT                      #206=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28093856 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "MspA1I: 6 sites") 3 3 "CMGCKG" 
COMMENT                            "GKCGMC") 
COMMENT                      #207=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28094088 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #208=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #40# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #209=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #40# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 679 " 1) 
COMMENT                                      #210=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #40# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 836 " 1))))) "NaeI: 2 sites") 
COMMENT                            3 3 "GCCGGC" "CGGCCG") 
COMMENT                      #211=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28060232 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #212=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #41# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #213=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 789 " 1) 
COMMENT                                      #214=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #41# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 832 " 1))))) "NarI: 2 sites") 
COMMENT                            2 4 "GGCGCC" "CCGCGG") 
COMMENT                      #215=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28107336 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NciI: 14 sites") 2 3 "CCSGG" 
COMMENT                            "GGSCC") 
COMMENT                      #216=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28117064 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #217=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #43# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #218=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #43# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3867 " 1) 
COMMENT                                      #219=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #43# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 6489 " 1))))) "NcoI: 2 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
COMMENT                      #220=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28114712 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NdeI: 4 sites") 2 4 "CATATG" 
COMMENT                            "GTATAC") 
COMMENT                      #221=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28107128 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #222=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #45# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #223=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #45# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 677 " 1) 
COMMENT                                      #224=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #45# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 834 " 1))))) "NgoMIV: 2 sites") 
COMMENT                            1 5 "GCCGGC" "CGGCCG") 
COMMENT                      #225=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28114952 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NheI: 0 sites") 1 5 "GCTAGC" 
COMMENT                            "CGATCG") 
COMMENT                      #226=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28106808 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #227=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #47# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #228=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #47# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3011 " 1))))) "NotI: 1 site") 
COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                      #229=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28046696 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "NruI: 0 sites") 3 3 "TCGCGA" 
COMMENT                            "AGCGCT") 
COMMENT                      #230=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28101560 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #231=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #49# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #232=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #49# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3782 " 1))))) "NsiI: 1 site") 
COMMENT                            5 1 "ATGCAT" "TACGTA") 
COMMENT                      #233=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28109792 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PacI: 0 sites") 5 3 "TTAATTAA" 
COMMENT                            "AATTAATT") 
COMMENT                      #234=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28096984 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PmeI: 0 sites") 4 4 "GTTTAAAC" 
COMMENT                            "CAAATTTG") 
COMMENT                      #235=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28107992 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "PstI: 3 sites") 5 1 "CTGCAG" 
COMMENT                            "GACGTC") 
COMMENT                      #236=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28107888 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #237=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #53# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #238=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #53# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 5557 " 1))))) "PvuI: 1 site") 
COMMENT                            4 2 "CGATCG" "GCTAGC") 
COMMENT                      #239=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28111840 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #240=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #54# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #241=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #54# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 1874 " 1) 
COMMENT                                      #242=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #54# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 2896 " 1))))) "PvuII: 2 sites") 
COMMENT                            3 3 "CAGCTG" "GTCGAC") 
COMMENT                      #243=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28108304 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "RsaI: 9 sites") 2 2 "GTAC" "CATG") 
COMMENT                      #244=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28114888 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SacI: 3 sites") 5 1 "GAGCTC" 
COMMENT                            "CTCGAG") 
COMMENT                      #245=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28078000 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #246=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #57# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #247=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #57# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 396 " 1))))) "SacII: 1 site") 
COMMENT                            4 2 "CCGCGG" "GGCGCC") 
COMMENT                      #248=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28078368 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #249=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #58# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #250=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #58# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 6111 " 1))))) "SalI: 1 site") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
COMMENT                      #251=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28091432 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #252=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #59# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #253=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #59# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2820 " 1) 
COMMENT                                      #254=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #59# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 5667 " 1))))) "ScaI: 2 sites") 
COMMENT                            3 3 "AGTACT" "TCATGA") 
COMMENT                      #255=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28105256 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #256=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #60# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #257=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #60# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3913 " 1))))) "SfiI: 1 site") 
COMMENT                            8 5 "GGCCNNNNNGGCC" "CCGGNNNNNCCGG") 
COMMENT                      #258=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 28109064 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG" 
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FEATURES             Location/Qualifiers
     rep_origin      complement(4163..4573)
                     /vntifkey="33"
                     /label=ORI
     promoter        6502..1087
                     /vntifkey="30"
                     /label=AG
     CDS             complement(5116..5973)
                     /vntifkey="4"
                     /label=Amp\R
                     /note="ORF: Frame #1   Start: atg (2098)   Stop:  taa (2955)"
     polyA_signal    3109..3200
                     /vntifkey="25"
                     /label=b-globin\polyA
     3'UTR           3201..3557
                     /vntifkey="50"
                     /label=3'\FR\of\b-globin
     promoter        3636..3719
                     /vntifkey="29"
                     /label=Lac\promoter
     CDS             1109..3004
                     /vntifkey="4"
                     /label=A2G\Mx1
                     /note="NM_010846"
     promoter        6113..6490
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
     mutation        1253..1255
                     /vntifkey="62"
                     /label=K49A
                     /note="WT: AAG"
BASE COUNT     1578 a      1673 c      1857 g      1611 t        30 others
ORIGIN
        1 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc cgtgccccgc 
       61 tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga 
      121 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggctcg 
      181 tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct ttgtgcgggg 
      241 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggcccg 
      301 cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcgtgt 
      361 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg cgaggggaac 
      421 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcggcggtc 
      481 gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg 
      541 tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca 
      601 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaggggcg 
      661 cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta 
      721 tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctggcg gagccgaaat 
      781 ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg aagcggtgcg gcgccggcag 
      841 gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccatct 
      901 ccagcctcgg ggctgccgca gggggacggc tgccttcggg ggggacgggg cagggcgggg 
      961 ttcggcttct ggcgtgtgac cggcgggntc tagancctct gctaaccatg ttcatgcctt 
     1021 cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg 
     1081 caaagaattc ctcgagaatt cgattacgat ggattctgtg aataatctgt gcaggcacta 
     1141 tgaggagaag gtgcggccct gtattgacct catcgacacc ctgagggctc tgggtgtgga 
     1201 gcaggacctg gccctgcctg ccatcgctgt cattggggac cagagttcag gggcgagctc 
     1261 tgttctggaa gcactgtctg gagtggccct ccccagaggc agtggtattg tcaccagatg 
     1321 ccctctggtg ctgaaattga ggaagctgaa agaaggagag gagtggagag gcaaagtctc 
     1381 ctatgatgac atcgaagtgg agctctctga tccttcagag gtggaagagg ccattaacaa 
     1441 gggtcagaac ttcattgctg gggtaggcct ggggatcagt gataagctca ttagcctgga 
     1501 tgtcagctcc ccaaatgtcc cagacctgac tctcattgac ctgcctggaa ttaccagggt 
     1561 ggctgtaggc aaccagcctg cagacatagg acgccagatc aagagactta tcaagacata 
     1621 catccaaaaa caagagacca tcaacctggt ggtagtcccc agcaatgtgg acattgctac 
     1681 cacagaggct ctgagcatgg ctcaggaggt ggaccctgaa ggggatagga ccataggggt 
     1741 cttgaccaag cctgatctgg tggacagagg tgctgaaggc aaggtcttgg atgtgatgcg 
     1801 gaacctggtg tatcctctga agaagggcta catgatagtc aagtgcagag gtcagcagga 
     1861 catccaagag cagctgagcc tgactgaggc ttttcagaaa gagcaagtct tcttcaagga 
     1921 tcactcatac ttcagcattc ttctggaaga tgggaaggcc acagtgccct gcctggcaga 
     1981 gagactgact gaggagctca cctcccacat ctgtaaatca ctgccactat tggaagatca 
     2041 aataaatagc agtcatcaga gtgcaagcga ggagctgcag aagtacggtg cagacatacc 
     2101 agaagatgac agaacgagga tgtcctttct ggtgaataaa atcagtgcct tcaataggaa 
     2161 tatcatgaat ttgatacaag cacaggaaac cgtatcagag ggagacagcc ggttgtttac 
     2221 caaactgcga aatgagtttc ttgcttggga tgatcatatt gaggaatatt ttaaaaaaga 
     2281 ttctcctgag gtacaaagca agatgaaaga atttgaaaat cagtatcgtg gccgggagct 
     2341 gccagggttt gtggactaca aggcatttga gagcatcatc aaaaagcgag tcaaagccct 
     2401 ggaagagtct gctgtgaaca tgctgcgcag ggtcactaag atggtccaaa ctgccttcgt 
     2461 aaagatttta tcaaatgatt ttggtgattt tttaaacctc tgctgtactg ctaagtccaa 
     2521 aattaaagaa ataagattaa accaagaaaa ggaagctgaa aacctgatcc gacttcactt 
     2581 ccagatggaa cagattgtct actgccagga ccaggtttac aaggaaacct tgaagacgat 
     2641 cagagagaag gaagctgaga aagagaagac caaggcatta ataaaccctg ctacctttca 
     2701 aaataactct cagtttcctc aaaaggggtt gactaccact gagatgaccc agcacctgaa 
     2761 agcctactac caggagtgca gacggaatat tgggagacag atccctctga ttatccagta 
     2821 cttcatcctg aaaacatttg gggaggaaat agagaaaatg atgcttcagc ttttacagga 
     2881 caccagtaag tgcagctggt tcctggagga gcagagtgac accagagaga agaagaagtt 
     2941 cctgaaaagg cggcttttaa ggctggatga ggctcggcag aagcttgcca aattctccga 
     3001 ttaaatttgc ggccgcacgc gttcgaggaa ttcactcctc aggtgcaggc tgcctatcag 
     3061 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc 
     3121 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa 
     3181 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg 
     3241 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca 
     3301 acatatgcca tatgctggct gccatgaaca aaggtggcta taaagaggtc atcagtatat 
     3361 gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat 
     3421 tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca 
     3481 tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct 
     3541 tctcttatga agatcnnnnc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc 
     3601 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 
     3661 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 
     3721 cactgcccgc tttccagtcg ggaaacctgt cgtgccagnn nnnnnnnnng gatccnatgc 
     3781 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 
     3841 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 
     3901 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 
     3961 taggcttttg caaaaagctn aacttgttta ttgcagctta taatggttac aaataaagca 
     4021 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     4081 ccaaactcat caatgtatct tatcatgtct ggatccnctg cattaatgaa tcggccaacg 
     4141 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     4201 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     4261 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     4321 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     4381 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     4441 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     4501 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 
     4561 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     4621 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     4681 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     4741 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 
     4801 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     4861 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     4921 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     4981 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     5041 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     5101 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     5161 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     5221 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     5281 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     5341 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     5401 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 
     5461 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     5521 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     5581 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     5641 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     5701 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 
     5761 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     5821 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     5881 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     5941 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     6001 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     6061 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgnnnnng tcgacattga 
     6121 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 
     6181 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     6241 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     6301 tgacgtcaat gggtggacta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     6361 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     6421 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     6481 gctattacca tggnnnnngt cgaggtgagc cccacgttct gcttcactct ccccatctcc 
     6541 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg 
     6601 ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg 
     6661 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt 
     6721 tatggcgagg cggcggcggc ggcggccct 
//
