LOCUS       pCSN43      5372 bp    DNA     circular UNA 04-okt.-2021
DEFINITION  LMBP 8047.
COMMENT     Accession number: LMBP 8047
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 04-OCT-2021
            NGS data implemented
FEATURES             Location/Qualifiers
     rep_origin      complement(3..458)
                     /label=f1\ori
     promoter        623..639
                     /label=T7\promoter
     promoter        683..1040
                     /label=trpC\promoter
     CDS             1041..2066
                     /label=hygromycin\resistance
     terminator      2236..2807
                     /label=trpC\terminator
     promoter        complement(3186..3202)
                     /label=T3\promoter
     RBS             complement(3228..3265)
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(3266..3349)
                     /label=lac\promoter
     rep_origin      complement(3441..3843)
                     /label=pMB1\ori
     CDS             complement(4384..5244)
                     /label=ampicillin\resistance
ORIGIN
        1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
       61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
      121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
      181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
      241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
      301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
      361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
      421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG
      481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG
      541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG
      601 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATAG GGCGAATTGG GTACCGGGCC
      661 CCCCCTCGAG GTCGACAGAA GATGATATTG AAGGAGCACT TTTTGGGCTT GGCTGGAGCT
      721 AGTGGAGGTC AACAATGAAT GCCTATTTTG GTTTAGTCGT CCAGGCGGTG AGCACAAAAT
      781 TTGTGTCGTT TGACAAGATG GTTCATTTAG GCAACTGGTC AGATCAGCCC CACTTGTAGC
      841 AGTAGCGGCG GCGCTCGAAG TGTGACTCTT ATTAGCAGAC AGGAACGAGG ACATTATTAT
      901 CATCTGCTGC TTGGTGCACG ATAACTTGGT GCGTTTGTCA AGCAAGGTAA GTGAACGACC
      961 CGGTCATACC TTCTTAAGTT CGCCCTTCCT CCCTTTATTT CAGATTCAAT CTGACTTACC
     1021 TATTCTACCC AAGCATCGAT ATGAAAAAGC CTGAACTCAC CGCGACGTCT GTCGAGAAGT
     1081 TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG GGCGAAGAAT
     1141 CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA AATAGCTGCG
     1201 CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC GCGCTCCCGA
     1261 TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC ATCTCCCGCC
     1321 GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT GTTCTGCAGC
     1381 CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG AGCGGGTTCG
     1441 GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC ATATGCGCGA
     1501 TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC AGTGCGTCCG
     1561 TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA GTCCGGCACC
     1621 TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC ATAACAGCGG
     1681 TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC AACATCTTCT
     1741 TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC
     1801 CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC
     1861 TCTATCAGAG CTTGGTTGAC GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG
     1921 ACGCAATCGT CCGATCCGGA GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG
     1981 CGGCCGTCTG GACCGATGGC TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA
     2041 GCACTCGTCC GAGGGCAAAG GAATAGAGTA GATGCCGACC GGGATCCACT TAACGTTACT
     2101 GAAATCATCA AACAGCTTGA CGAATCTGGA TATAAGATCG TTGGTGTCGA TGTCAGCTCC
     2161 GGAGTTGAGA CAAATGGTGT TCAGGATCTC GATAAGATAC GTTCATTTGT CCAAGCAGCA
     2221 AAGAGTGCCT TCTAGTGATT TAATAGCTCC ATGTCAACAA GAATAAAACG CGTTTCGGGT
     2281 TTACCTCTTC CAGATACAGC TCATCTGCAA TGCATTAATG CATTGGACCT CGCAACCCTA
     2341 GTACGCCCTT CAGGCTCCGG CGAAGCAGAA GAATAGCTTA GCAGAGTCTA TTTTCATTTT
     2401 CGGGAGACGA GATCAAGCAG ATCAACGGTC GTCAAGAGAC CTACGAGACT GAGGAATCCG
     2461 CTCTTGGCTC CACGCGACTA TATATTTGTC TCTAATTGTA CTTTGACATG CTCCTCTTCT
     2521 TTACTCTGAT AGCTTGACTA TGAAAATTCC GTCACCAGCC CCTGGGTTCG CAAAGATAAT
     2581 TGCACTGTTT CTTCCTTGAA CTCTCAAGCC TACAGGACAC ACATTCATCG TAGGTATAAA
     2641 CCTCGAAAAT CATTCCTACT AAGATGGGTA TACAATAGTA ACCATGGTTG CCTAGTGAAT
     2701 GCTCCGTAAC ACCCAATACG CCGGCCGAAA CTTTTTTACA ACTCTCCTAT GAGTCGTTTA
     2761 CCCAGAATGC ACAGGTACAC TTGTTTAGAG GTAATCCTTC TTTCTAGAGG ATCCTCTACG
     2821 CCGGACGCAT CGTGGCCGGC ATCACCGGCG CCACAGGTGC GGTTGCTGGC GCCTATATCG
     2881 CCGACATCAC CGATGGGGAA GATCGGGCTC GCCACTTCGG GCTCATGAGC GCTTGTTTCG
     2941 GCGTGGGTAT GGTGGCAGGC CCCGTGGCCG GGGGACTGTT GGGCGCCATC TCCTTGCATG
     3001 CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC TGCTTCCTAA
     3061 TGCAGGAGTC GCATAAGGGA GAGCGTCGAC GGTATCGATA AGCTTGATAT CGAATTCCTG
     3121 CAGCCCGGGG GATCCACTAG TTCTAGAGCG GCCGCCACCG CGGTGGAGCT CCAGCTTTTG
     3181 TTCCCTTTAG TGAGGGTTAA TTTCGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT
     3241 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA
     3301 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC
     3361 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG
     3421 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT
     3481 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA
     3541 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG
     3601 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     3661 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     3721 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     3781 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     3841 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     3901 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     3961 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     4021 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     4081 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     4141 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     4201 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA
     4261 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
     4321 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA
     4381 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC
     4441 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG
     4501 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT
     4561 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
     4621 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG
     4681 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC
     4741 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA
     4801 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC
     4861 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
     4921 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG
     4981 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT
     5041 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG
     5101 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC
     5161 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
     5221 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA
     5281 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG
     5341 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC AC
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