LOCUS       pCB1003                 4123 bp    DNA     circular     22-JAN-2013
DEFINITION  LMBP 8050.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8050
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 22-JAN-2013
            
COMMENT     VNTDATE|650983592|
COMMENT     VNTNAME|pCB1003|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCB1003" 0 4123 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 150 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "BamHI" "EcoRI" "HpaI" "NdeI" "PvuI" "SalI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 397 0 1834 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NdeI" "catatg" 3 0 2 185 0 1225 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 280 0 1139 0 3507 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HpaI" "gttaac" 4 0 2 411 0 1824 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SalI" "gtcgac" 2 0 3 403 0 1828 0 1867 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1855 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 1 1907 1944 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(1913..1950)") 
COMMENT        #7=(CFSignal (CObList) "lac promoter" 30 0 1 1945 2028 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1951..2034)") 
COMMENT        #8=(CFSignal (CObList) "pMB1 ori" 33 0 1 2120 2522 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2126..2528)") 
COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3063 3923 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(3069..3929)") 
COMMENT        #10=(CFSignal (CObList) "hygromycin resistance" 4 0 0 774 1799 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "780..1805") 
COMMENT        #11=(CFSignal (CObList) "trpC promoter" 29 0 0 408 770 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCB1003'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 01/14/13 12:46" 1) 
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COMMENT                          "Last Modified: 01/22/13 02:41" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4123 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 16-JAN-2013" 1))) 
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FEATURES             Location/Qualifiers
     RBS             complement(1907..1944)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(1945..2028)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(2120..2522)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3063..3923)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             774..1799
                     /vntifkey="4"
                     /label=hygromycin\resistance
     promoter        408..770
                     /vntifkey="29"
                     /label=trpC\promoter
BASE COUNT      979 a      1047 c      1101 g       996 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgtcgacgtt aacagaagat 
      421 gatattgaag gagcattttt gggcttggct ggagctagtg gaggtcaaca catcaatgct 
      481 attttggttt agtcgtccag gcggatcaca aaatttgtgt cgtttgacaa gatggttcat 
      541 ttaggcaact ggtcagatca gcccacttgt aagcagtagc ggcggcgctc gaagtgtgac 
      601 tcttattagc agacaggaac gaggacatta ttatcatctg ctgcttggtg cacgataact 
      661 tgtgcgtttg tcaagcaagg taagtgaacg acccggtcat accttcttaa gttcgccctt 
      721 cctcccttta tttcagattc aatctgactt acctattcta ccgaagcatc caaatgaaaa 
      781 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct 
      841 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag 
      901 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg 
      961 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt 
     1021 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc 
     1081 tgcctgaaac cgaactgccc gctgttctgg agccggtcgc ggaggccatg gatgcgatcg 
     1141 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc 
     1201 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc 
     1261 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc 
     1321 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca 
     1381 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg 
     1441 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg 
     1501 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcgcctcc 
     1561 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt 
     1621 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga 
     1681 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag 
     1741 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga 
     1801 gtagatgccg accgggttcc gttaacgtcg acgaattcga gctcggtacc cggggatcct 
     1861 ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg 
     1921 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 
     1981 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 
     2041 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 
     2101 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     2161 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     2221 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     2281 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     2341 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     2401 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     2461 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     2521 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     2581 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     2641 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     2701 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     2761 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     2821 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     2881 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     2941 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     3001 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     3061 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     3121 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     3181 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     3241 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     3301 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     3361 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     3421 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     3481 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     3541 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     3601 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     3661 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 
     3721 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     3781 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     3841 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     3901 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     3961 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     4021 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 
     4081 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc 
//
