LOCUS       pOSCAR                  9422 bp    DNA     circular     17-JAN-2013
DEFINITION  LMBP 8055.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8055
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-JAN-2013
COMMENT     VNTDATE|650972316|
COMMENT     VNTNAME|pOSCAR|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 4 774 0 1878 0 7374 0 
COMMENT            8664 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuII" "cagctg" 4 0 1 3687 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 4 2139 0 3583 0 4294 0 
COMMENT            4556 0 0 "")) 
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COMMENT        #6=(CFSignal (CObList) "attP2R" 0 0 1 4061 4292 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "chloramphenicol resistance" 4 0 1 3141 3800 0 
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COMMENT                          "DNA Plasmid 'pOSCAR'" 1) 
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COMMENT                          "Created: 01/14/13 09:38" 1) 
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FEATURES             Location/Qualifiers
     rep_origin      complement(7272..1615)
                     /vntifkey="33"
                     /label=pVS1\ori
     attenuator      complement(1906..2137)
                     /vntifkey="0"
                     /label=attP3
     attenuator      complement(4061..4292)
                     /vntifkey="0"
                     /label=attP2R
     CDS             complement(3141..3800)
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             complement(2494..2799)
                     /vntifkey="4"
                     /label=ccdB
     rep_origin      6586..6988
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             5001..5972
                     /vntifkey="4"
                     /label=streptomycin/spectinomycin\resistance\(aadA)
BASE COUNT     2133 a      2540 c      2435 g      2314 t 
ORIGIN
        1 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga 
       61 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc 
      121 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg 
      181 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc 
      241 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg 
      301 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga 
      361 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt 
      421 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat 
      481 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag 
      541 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc 
      601 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa 
      661 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc 
      721 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc 
      781 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg 
      841 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc 
      901 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg 
      961 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc 
     1021 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg 
     1081 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg 
     1141 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg 
     1201 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca 
     1261 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc 
     1321 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg 
     1381 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc 
     1441 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag 
     1501 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca 
     1561 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc 
     1621 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg 
     1681 gataaacctt ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta 
     1741 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga 
     1801 caatctgatc caagctcaag ctaagcttca ggaaacagct atgaccatgt aatacgactc 
     1861 actatagggg atatcgcggc cgcgttaacg ctaccatgga gctccaaata atgattttat 
     1921 tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt tttataatgc 
     1981 caactttgta taataaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa 
     2041 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atgcagtcac 
     2101 tatgaatcaa ctacttagat ggtattagtg acctgtagaa ttcgagctcg accgacagcc 
     2161 ttccaaatgt tcttctcaaa cggaatcgtc gtatccagcc tactcgctat tgtcctcaat 
     2221 gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac 
     2281 aaaataaaaa catctaccta ttcatatacg ctagtgtcat agtcctgaaa atcatctgca 
     2341 tcaagaacaa tttcacaact cttatacttt tctcttacaa gtcgttcggc ttcatctgga 
     2401 ttttcagcct ctatacttac taaacgtgat aaagtttctg taatttctac tgtatcgacc 
     2461 tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat 
     2521 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga 
     2581 gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac 
     2641 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat 
     2701 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata 
     2761 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagtcc ctgttctcgt 
     2821 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt 
     2881 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca 
     2941 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt 
     3001 cactgtaata cgctgcttca tagcacacct ctttttgaca tacttcgggt atacatatca 
     3061 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggctttt 
     3121 agtaagccgg atccacgcga ttacgccccg ccctgccact catcgcagta ctgttgtaat 
     3181 tcattaagca ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc 
     3241 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg 
     3301 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg 
     3361 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg 
     3421 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca 
     3481 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca 
     3541 ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc 
     3601 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg 
     3661 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact 
     3721 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat 
     3781 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa 
     3841 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga 
     3901 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca 
     3961 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcggc gcaaagtgcg 
     4021 tcgggtgatg ctgccaactt agtcgacgct ctagagctcc aaataatgat tttattttga 
     4081 ctgatagtga cctgttcgtt gcaacaaatt gataagcaat gcttttttat aatgccaact 
     4141 ttgtacaaga aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag 
     4201 attttgcata aaaaacagac tacataatac tgtaaaacac aacatatgca gtcactatga 
     4261 atcaactact tagatggtat tagtgacctg tagaattcga gctctagagc tgcagggccc 
     4321 gagcttaaga ctggccgtcg ttttacggta ccgcgatcgc tcgcgacctg caggcataaa 
     4381 gccgtcagtg tccgcataaa gaaccaccca taatacccat aatagctgtt tgccatcgct 
     4441 accttaggac cgttatagtt aattaccctg ttatccctat taattaagag ctcgctacct 
     4501 taagagagga tatcggcgcg cctctagaat ttaaatggat cctacgtact cgaggaattc 
     4561 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc 
     4621 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg 
     4681 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc 
     4741 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg 
     4801 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt 
     4861 aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc 
     4921 ctcgttcggt tcgtaagctg taatgcaagt agcgtaactg ccgtcacgca actggtccag 
     4981 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttcttgtta 
     5041 tgacatgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg 
     5101 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc 
     5161 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat 
     5221 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 
     5281 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 
     5341 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 
     5401 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 
     5461 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 
     5521 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 
     5581 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 
     5641 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 
     5701 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 
     5761 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 
     5821 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac 
     5881 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga 
     5941 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga 
     6001 cgccgcttcg ccggcgttaa ctcaagcgat tagatgcact aagcacataa ttgctcacag 
     6061 ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac 
     6121 aaattgggag atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga 
     6181 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 
     6241 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 
     6301 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 
     6361 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 
     6421 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 
     6481 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 
     6541 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 
     6601 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 
     6661 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 
     6721 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 
     6781 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 
     6841 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 
     6901 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 
     6961 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg 
     7021 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag 
     7081 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc 
     7141 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 
     7201 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 
     7261 gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt 
     7321 ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg 
     7381 ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt 
     7441 tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga 
     7501 gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca 
     7561 gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc 
     7621 cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct 
     7681 tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa 
     7741 ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc 
     7801 tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc 
     7861 acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg 
     7921 aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg 
     7981 ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg 
     8041 ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc 
     8101 ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc 
     8161 accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac 
     8221 cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac 
     8281 ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc 
     8341 cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc 
     8401 cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg 
     8461 tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc 
     8521 atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt 
     8581 tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg 
     8641 gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc 
     8701 tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc 
     8761 agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgtcacgccg attcctcggg 
     8821 cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta cgcctggcca 
     8881 accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt 
     8941 ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt 
     9001 actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta 
     9061 ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca 
     9121 tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac 
     9181 ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat 
     9241 gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg 
     9301 ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt 
     9361 gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc 
     9421 gt 
//
