LOCUS       pA-HYG-OSCAR            4833 bp    DNA     circular     17-JAN-2013
DEFINITION  LMBP 8056.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8056
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 17-JAN-2013
COMMENT     VNTDATE|650972138|
COMMENT     VNTNAME|pA-HYG OSCAR|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pA-HYG OSCAR" 0 4833 
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COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
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COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #2=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 665 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 2607 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "T7 promoter" 30 0 0 626 642 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "attP4" 0 0 1 678 909 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "hygromycin resistance" 4 0 1 959 1984 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "attP1R" 0 0 1 2361 2592 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "T3 promoter" 30 0 1 2647 2663 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pA-HYG OSCAR'" 1) 
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COMMENT                          1) 
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COMMENT                          "Created: 01/14/13 09:35" 1) 
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COMMENT                          "Last Modified: 01/17/13 11:24" 1) 
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COMMENT                          "length: 4833 bp" 1) 
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FEATURES             Location/Qualifiers
     rep_origin      complement(3..458)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        626..642
                     /vntifkey="30"
                     /label=T7\promoter
     attenuator      complement(678..909)
                     /vntifkey="0"
                     /label=attP4
     CDS             complement(959..1984)
                     /vntifkey="4"
                     /label=hygromycin\resistance
     attenuator      complement(2361..2592)
                     /vntifkey="0"
                     /label=attP1R
     promoter        complement(2647..2663)
                     /vntifkey="30"
                     /label=T3\promoter
     RBS             complement(2689..2726)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        complement(2727..2810)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(2902..3304)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3845..4705)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        complement(1991..2342)
                     /vntifkey="29"
                     /label=trpC\promoter
BASE COUNT     1224 a      1209 c      1188 g      1212 t 
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 
      661 ccgcggtgcc ggaattctac aggtcactaa taccatctaa gtagttgatt catagtgact 
      721 gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata 
      781 tattgatatt tatatcattt tacgtttctc gttcaacttt tctatacaaa gttggcatta 
      841 taaaaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa 
      901 tcattatttg gccgctctag aactagtgga tccaactggt tcccggtcgg catctactct 
      961 attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta 
     1021 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc 
     1081 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat 
     1141 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga 
     1201 ggcgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca 
     1261 tacaagccaa ccacggcctc cagaagagga tgttggcgac ctcgtattgg gaatccccga 
     1321 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt 
     1381 tggagccgaa atccgcatgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca 
     1441 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc 
     1501 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac 
     1561 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga 
     1621 tcgcatccat ggcctccgcg accggctgga gaacagcggg cagttcggtt tcaggcaggt 
     1681 cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaact 
     1741 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat 
     1801 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc 
     1861 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga 
     1921 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt 
     1981 tcatttggat gcttgggtag aataggtaag tcagattgaa tctgaaataa agggaggaag 
     2041 ggcgaactta agaaggtatg accgggtcgt ccacttacct tgcttgacaa acgcaccaag 
     2101 ttatcgtgca ccaagcagca gatgataata atgtcctcgt tcctgtctgc taataagagt 
     2161 cacacttcga gcgccgccgc tactgctaca agtggggctg atctgaccag ttgcctaaat 
     2221 gaaccatctt gtcaaacgac acaaattttg tgctcaccgc ctggacgact aaaccaaaat 
     2281 aggcattcat tgttgacctc cactagctcc agccaagccc aaaaaatgct ccttcaatat 
     2341 cagttgggct gcaggaattc tacaggtcac taataccatc taagtagttg attcatagtg 
     2401 actgcatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta 
     2461 atatattgat atttatatca ttttacgttt ctcgttcaac ttttttgtac aaagttggca 
     2521 ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata 
     2581 aaatcattat ttggagctct agagcgtcga cctcgagggg gggcccggta cccagctttt 
     2641 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg 
     2701 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     2761 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     2821 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 
     2881 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     2941 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     3001 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     3061 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     3121 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     3181 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     3241 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     3301 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     3361 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     3421 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     3481 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
     3541 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     3601 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     3661 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     3721 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     3781 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     3841 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     3901 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     3961 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     4021 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     4081 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     4141 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     4201 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     4261 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     4321 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     4381 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     4441 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     4501 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     4561 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     4621 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     4681 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     4741 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     4801 gggttccgcg cacatttccc cgaaaagtgc cac 
//
