LOCUS       Cre_excised_pRMCE_DV1-mMESP1        6600 bp    DNA     circular SYN 19-MAR-1980
DEFINITION  LMBP 8177.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8177
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|Cre_excised_pRMCE_DV1-mMESP1|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT            (CStringList) 1 1 1 1 "") 
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COMMENT        #9=(CFSignal (CObList) "mMESP1" 4 0 0 54 782 0 (CStringList) 
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FEATURES             Location/Qualifiers
     Misc._recombina join(6594..6600,1..14)
                     /vntifkey="21"
                     /label=attB1
                     /note="Unknown feature type:Misc._recombina"
     CDS             1454..2170
                     /vntifkey="4"
                     /label=EGFP
     misc_feature    2508..2750
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    2751..2993
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    3543..3590
                     /vntifkey="21"
                     /label=FRT\mutant
     CDS             complement(4796..5656)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    6274..6321
                     /vntifkey="21"
                     /label=FRT
     promoter        complement(3678..3761)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(3853..4255)
                     /vntifkey="33"
                     /label=pMB1\ori
     misc_feature    6338..6441
                     /vntifkey="21"
                     /label=SA
     misc_feature    complement(6504..6537)
                     /vntifkey="21"
                     /label=loxP
     attenuator      6593..17
                     /vntifkey="0"
                     /label=attB1
     CDS             54..782
                     /vntifkey="4"
                     /label=mMESP1
     misc_marker     783..806
                     /vntifkey="22"
                     /label=FLAG\epitope
     misc_marker     807..830
                     /vntifkey="22"
                     /label=FLAG\epitope
     misc_marker     831..854
                     /vntifkey="22"
                     /label=FLAG\epitope
     attenuator      complement(879..903)
                     /vntifkey="0"
                     /label=attB2
     RBS             978..1441
                     /vntifkey="32"
                     /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES)
     promoter        3012..3519
                     /vntifkey="29"
                     /label=mPGK1\promoter
     terminator      2204..2428
                     /vntifkey="43"
                     /label=BGH\polyA
BASE COUNT     1473 a      1899 c      1818 g      1410 t 
ORIGIN
        1 gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac tggatccggt accatggccc 
       61 agcccctgtg cgagccgcgc tccgagtcct ggatcctgag tcccgctggt cggcagccac 
      121 cgatgccttc cgatgggaac agcgtctgct ccccagcctg gtcctcggac ccgtgggacg 
      181 gtgcccaggc cagcagccct gcaccaccct gcgcccgccc ggcccggcgt gctgggaccc 
      241 cgggtaggcg cgggacgcac ggtagccgcc tgggtagcgg acagcggcag agcgccagcg 
      301 agcgggagaa gctacgtatg cgcacactcg cccgcgcgct gcacgagctg cgccgcttct 
      361 tgccgccatc cgtggcacca accggccaga acctgaccaa gatcgagacg ctgcgcctgg 
      421 ccatccgcta cattggccac ctgtcggctg tgctgggact cagcgaggac aacctccggc 
      481 gacagcggca cgcggtgtca cctcgaggct gcccgctgtg ccccgacagc gacctggcgc 
      541 agtcgcagtc actcggtcct ggtttaagcc cggccgtctg cagcggggtg tcgtggggat 
      601 ccccgcctgc ctaccctaga ccccgagtcg ccgcagaatc gtgggaccca tcgttccagt 
      661 acgcagaaac agcatcccag gaaaggcagg aaatggagcc cagtccctca tctccgctct 
      721 tcagcagcga catgctggct cttctagaaa cctggacgcc gccgcaggag tggccgcctg 
      781 ccgattacaa ggatgacgat gacaaggatt acaaggatga cgatgacaag gattacaagg 
      841 atgacgatga caagtgaggc cgcactcgag atatctagac ccagctttct tgtacaaagt 
      901 ggtgattcga ggtcgacggt atcgataagc ttgatatcga attccgcccc tctccctccc 
      961 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgagcg tttgtctata 
     1021 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccgaaac ctggccctgt 
     1081 cttcttgacg acgattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt 
     1141 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaacaac gtctgtagcg 
     1201 accctttgca ggcagcggaa ccccccacct ggcgacatgg atagttgtgg aaagagtcaa 
     1261 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg 
     1321 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa 
     1381 aaacgtctag gccccccgaa ccacggggac gtggttttgg tttgaaaaac acgatgataa 
     1441 tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 
     1501 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 
     1561 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 
     1621 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 
     1681 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 
     1741 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 
     1801 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 
     1861 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 
     1921 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 
     1981 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 
     2041 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 
     2101 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 
     2161 gctgtacaag taaagcggcc gcgagctcgc tgatcagcct cgactgtgcc ttctagttgc 
     2221 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 
     2281 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 
     2341 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 
     2401 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg 
     2461 atcctctagt tggcgcgccg ggccctcgcg actcgaggaa taaattcgag ctcacgggga 
     2521 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca 
     2581 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc 
     2641 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc 
     2701 tcgctgctct ttgagcctgc agacacctgg gggatacggg gaaaaagctt gagctcacgg 
     2761 ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca 
     2821 gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc 
     2881 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc 
     2941 ttctcgctgc tctttgagcc tgcagacacc tgggggatac ggggaaaaag cttgtcgacg 
     3001 aataaattca attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg 
     3061 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt 
     3121 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt 
     3181 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt 
     3241 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat 
     3301 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg 
     3361 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg 
     3421 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg 
     3481 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcaatcgc cgccaccatg 
     3541 gagaagttcc tattccgaag ttcctattct tcaaaaggta taggaacttc cgatcgggat 
     3601 ccccaatcct gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 
     3661 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 
     3721 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 
     3781 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 
     3841 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 
     3901 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     3961 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     4021 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     4081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     4141 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     4201 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     4261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     4321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     4381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     4441 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 
     4501 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     4561 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     4621 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     4681 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     4741 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     4801 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     4861 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     4921 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     4981 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     5041 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     5101 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     5161 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     5221 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     5281 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     5341 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     5401 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     5461 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     5521 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     5581 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     5641 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 
     5701 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
     5761 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 
     5821 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 
     5881 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 
     5941 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 
     6001 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 
     6061 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 
     6121 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 
     6181 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 
     6241 cgacggccag tgaattccac aaaagtgcga tcggaagttc ctattccgaa gttcctattc 
     6301 tctagaaagt ataggaactt cttaattaag ggatctgtag ggcgcagtag tccagggttt 
     6361 ccttgatgat gtcatactta tcctgtccct tttttttcca cagctcgcgg ttgaggacaa 
     6421 actcttcgcg gtctttccag tggggatcga cggtatcgta gagtcgaggc cgctctagaa 
     6481 ctagtggatc cggaaccctt aatataactt cgtataatgt atgctatacg aagttattag 
     6541 gtccctcgac ctgcagccca agctagctta tcgataccgt cgacctcgaa tcacaagttt 
//
