LOCUS       pRMCE-DV2               6391 bp    DNA     circular UNA 21-DEC-2016
DEFINITION  LMBP 8190.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-11555561|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRMCE-DV2|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     misc_feature    418..465
                     /vntifkey="21"
                     /label=FRT
     misc_feature    490..732
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    746..988
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     attenuator      2820..2944
                     /vntifkey="0"
                     /label=attR3
     attenuator      complement(1099..1223)
                     /vntifkey="0"
                     /label=attR4
     CDS             2474..2779
                     /vntifkey="4"
                     /label=ccdB
     CDS             1473..2132
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     misc_feature    3043..3285
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    3286..3528
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    4078..4125
                     /vntifkey="21"
                     /label=FRT\mutant
     CDS             complement(5331..6191)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        3547..4054
                     /vntifkey="29"
                     /label=mPGK1\promoter
     promoter        complement(4213..4296)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(4388..4790)
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     1530 a      1677 c      1683 g      1501 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccacaaaagt gcgatcggaa 
      421 gttcctattc cgaagttcct attctctaga aagtatagga acttcttaat taagcggccg 
      481 cggcgcgccg agctcacggg gacagccccc ccccaaagcc cccagggatg taattacgtc 
      541 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc 
      601 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg 
      661 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct gggggatacg 
      721 gggaaaaagc ttggccaaaa aggccgagct cacggggaca gccccccccc aaagccccca 
      781 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct 
      841 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg 
      901 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag 
      961 acacctgggg gatacgggga aaaagctttt aattaagcgg ccgcggcgcg ccgggccctc 
     1021 gatcaagctt atcgataccg tcgacctcga agctcgtttt cccagtcacg acgttgtaaa 
     1081 acgacggcca gtgaattatc aactttgtat agaaaagttg aacgagaaac gtaaaatgat 
     1141 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa 
     1201 acacaacata tccagtcact atggcggccg ctaagttggc agcatcaccc gacgcacttt 
     1261 gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc 
     1321 ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg 
     1381 taagaggttc caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt 
     1441 tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca 
     1501 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc 
     1561 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga 
     1621 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc 
     1681 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc 
     1741 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc 
     1801 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa 
     1861 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct 
     1921 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg 
     1981 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc 
     2041 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac 
     2101 agtactgcga tgagtggcag ggcggggcgt aatctagagg atccggctta ctaaaagcca 
     2161 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt 
     2221 atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga 
     2281 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc 
     2341 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag 
     2401 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac 
     2461 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct 
     2521 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct 
     2581 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat 
     2641 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat 
     2701 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct 
     2761 gatgttctgg ggaatataaa tgtcaggctc cgttatacac agccagtctg caggtcgacc 
     2821 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct 
     2881 aatttaatat attgatattt atatcatttt acgtttctcg ttcagcttta ttatacaaag 
     2941 ttgatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctcg 
     3001 ggtcgacggt atcgataagc tagcgactcg aggaataaat tcgagctcac ggggacagcc 
     3061 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag 
     3121 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg 
     3181 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct 
     3241 gctctttgag cctgcagaca cctgggggat acggggaaaa agcttgagct cacggggaca 
     3301 gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc 
     3361 gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt 
     3421 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc 
     3481 gctgctcttt gagcctgcag acacctgggg gatacgggga aaaagcttgt cgacgaataa 
     3541 attcaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 
     3601 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca 
     3661 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact 
     3721 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa 
     3781 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag 
     3841 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag 
     3901 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc 
     3961 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg 
     4021 ctgttctcct cttcctcatc tccgggcctt tcgacctgca atcgccgcca ccatggagaa 
     4081 gttcctattc cgaagttcct attcttcaaa aggtatagga acttccgatc gggatcccca 
     4141 atcctgcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 
     4201 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 
     4261 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     4321 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     4381 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 
     4441 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 
     4501 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 
     4561 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 
     4621 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 
     4681 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 
     4741 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 
     4801 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 
     4861 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 
     4921 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 
     4981 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 
     5041 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 
     5101 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 
     5161 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 
     5221 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 
     5281 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 
     5341 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 
     5401 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 
     5461 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 
     5521 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 
     5581 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 
     5641 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 
     5701 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 
     5761 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 
     5821 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 
     5881 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 
     5941 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
     6001 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
     6061 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
     6121 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
     6181 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
     6241 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
     6301 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 
     6361 aaaaataggc gtatcacgag gccctttcgt c 
//
