LOCUS       Cre-excised_pRMCE_DV1        7394 bp    DNA     circular UNA  4-NOV-2013
DEFINITION  LMBP 8195.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8195
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 4-NOV-2013

COMMENT     VNTDATE|677782603|
COMMENT     VNTNAME|Cre excised pRMCE DV1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "Cre excised pRMCE DV1" 0 7394 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "EcoRI" "EcoRV" "PvuII" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 7107 7106 1 7394 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 3780 0 4567 0 5861 0 0 
COMMENT            "") #1=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 5857 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PvuII" "cagctg" 4 0 4 1328 0 3692 0 4467 0 
COMMENT            7372 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 4 30 0 532 0 2996 0 
COMMENT            6080 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2323..3183)") 
COMMENT        #5=(CFSignal (CObList) "loxP" 21 0 1 4031 4064 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4031..4064)") 
COMMENT        #6=(CFSignal (CObList) "attR1" 0 0 1 4120 4244 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4120..4244)") 
COMMENT        #7=(CFSignal (CObList) "attR2" 0 0 0 5700 5824 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5700..5824") 
COMMENT        #8=(CFSignal (CObList) "5' UTR of the EMCV polyprotein gene (IRES)" 32 0 
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COMMENT        #9=(CFSignal (CObList) "EGFP" 4 0 0 6375 7094 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "6375..7094") 
COMMENT        #10=(CFSignal (CObList) "lac promoter" 30 0 1 1205 1288 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 1380 1782 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "mPGK1 promoter" 29 0 0 539 1046 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "539..1046") 
COMMENT        #13=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 4252 4304 0 
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COMMENT        #14=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 4353 5012 0 
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COMMENT        #15=(CFSignal (CObList) "ccdB" 4 0 0 5354 5659 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "BGH polyA" 43 0 0 7125 7349 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) 
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COMMENT        #19=(CFSignal (CObList) "FRT mutant" 21 0 0 1070 1117 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 11/04/13 04:56" 1) 
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COMMENT                          "Last Modified: 11/04/13 05:06" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7394 bp" 1) 
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COMMENT                          "Division: UNA" 1) 
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COMMENT                          "Modification Date in the Original DB: 8-JUL-2013" 1))) 
COMMENT                   "Standard Fields") 
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FEATURES             Location/Qualifiers
     misc_feature    35..277
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    278..520
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    1070..1117
                     /vntifkey="21"
                     /label=FRT\mutant
     CDS             complement(2323..3183)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    3801..3848
                     /vntifkey="21"
                     /label=FRT
     misc_feature    complement(4031..4064)
                     /vntifkey="21"
                     /label=loxP
     attenuator      complement(4120..4244)
                     /vntifkey="0"
                     /label=attR1
     attenuator      5700..5824
                     /vntifkey="0"
                     /label=attR2
     RBS             5868..6362
                     /vntifkey="32"
                     /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES)
     CDS             6375..7094
                     /vntifkey="4"
                     /label=EGFP
     promoter        complement(1205..1288)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(1380..1782)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        539..1046
                     /vntifkey="29"
                     /label=mPGK1\promoter
     promoter        4252..4304
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             4353..5012
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             5354..5659
                     /vntifkey="4"
                     /label=ccdB
     terminator      7125..7349
                     /vntifkey="43"
                     /label=BGH\polyA
BASE COUNT     1770 a      1941 c      1944 g      1739 t 
ORIGIN
        1 cgcgccgggc cctcgcgact cgaggaataa attcgagctc acggggacag ccccccccca 
       61 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg 
      121 ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc 
      181 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg 
      241 agcctgcaga cacctggggg atacggggaa aaagcttgag ctcacgggga cagccccccc 
      301 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc 
      361 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac 
      421 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct 
      481 ttgagcctgc agacacctgg gggatacggg gaaaaagctt gtcgacgaat aaattcaatt 
      541 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc 
      601 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg 
      661 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct 
      721 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt 
      781 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg 
      841 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg 
      901 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag 
      961 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc 
     1021 ctcttcctca tctccgggcc tttcgacctg caatcgccgc caccatggag aagttcctat 
     1081 tccgaagttc ctattcttca aaaggtatag gaacttccga tcgggatccc caatcctgca 
     1141 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 
     1201 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 
     1261 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 
     1321 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 
     1381 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 
     1441 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 
     1501 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 
     1561 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 
     1621 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 
     1681 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 
     1741 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 
     1801 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 
     1861 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 
     1921 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 
     1981 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 
     2041 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 
     2101 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 
     2161 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 
     2221 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 
     2281 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 
     2341 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 
     2401 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 
     2461 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 
     2521 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 
     2581 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 
     2641 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 
     2701 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 
     2761 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 
     2821 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 
     2881 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 
     2941 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 
     3001 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 
     3061 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 
     3121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 
     3181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 
     3241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 
     3301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 
     3361 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 
     3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 
     3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 
     3541 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 
     3601 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 
     3661 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 
     3721 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 
     3781 attccacaaa agtgcgatcg gaagttccta ttccgaagtt cctattctct agaaagtata 
     3841 ggaacttctt aattaaggga tctgtagggc gcagtagtcc agggtttcct tgatgatgtc 
     3901 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc 
     3961 tttccagtgg ggatcgacgg tatcgtagag tcgaggccgc tctagaacta gtggatccgg 
     4021 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg 
     4081 cagcccaagc tagcttatcg ataccgtcga cctcgaatca caagtttgta caaaaaagct 
     4141 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa 
     4201 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca 
     4261 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc 
     4321 cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca 
     4381 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc 
     4441 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga 
     4501 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc 
     4561 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc 
     4621 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc 
     4681 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa 
     4741 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct 
     4801 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg 
     4861 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc 
     4921 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac 
     4981 agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca 
     5041 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt 
     5101 atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga 
     5161 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc 
     5221 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag 
     5281 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac 
     5341 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct 
     5401 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct 
     5461 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat 
     5521 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat 
     5581 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct 
     5641 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc 
     5701 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct 
     5761 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag 
     5821 tggtgattcg aggtcgacgg tatcgataag cttgatatcg aattccgccc ctctccctcc 
     5881 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgagc gtttgtctat 
     5941 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccgaaa cctggccctg 
     6001 tcttcttgac gacgattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt 
     6061 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaacaa cgtctgtagc 
     6121 gaccctttgc aggcagcgga accccccacc tggcgacatg gatagttgtg gaaagagtca 
     6181 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt 
     6241 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa 
     6301 aaaacgtcta ggccccccga accacgggga cgtggttttg gtttgaaaaa cacgatgata 
     6361 atatggccac aaccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 
     6421 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 
     6481 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 
     6541 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 
     6601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 
     6661 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 
     6721 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 
     6781 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 
     6841 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 
     6901 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 
     6961 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 
     7021 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 
     7081 agctgtacaa gtaaagcggc cgcgagctcg ctgatcagcc tcgactgtgc cttctagttg 
     7141 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 
     7201 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 
     7261 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 
     7321 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 
     7381 gatcctctag ttgg 
//
