ID pEntry\attR2-IRES-eGFP-luc+-pA-attL3 standard; circular DNA; ; 6273 BP. DE LMBP 8200. CC Accession number: LMBP 8200 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 30-JUL-2013 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator complement(2173..2200) FT /vntifkey="43" FT /label=T2\terminator FT terminator 2332..2375 FT /vntifkey="43" FT /label=T1\terminator FT promoter complement(149..165) FT /vntifkey="30" FT /label=T7\promoter FT CDS 299..1108 FT /vntifkey="4" FT /label=Tn903\neomycin\resistance FT attenuator complement(14..109) FT /vntifkey="0" FT /label=attL3 FT CDS 4062..5711 FT /vntifkey="4" FT /label=LUCm FT CDS 3321..4037 FT /vntifkey="4" FT /label=EGFP FT rep_origin 1625..2027 FT /vntifkey="33" FT /label=pMB1\ori FT terminator 5727..5818 FT /vntifkey="43" FT /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA FT misc_feature 5819..6175 FT /vntifkey="21" FT /label=3'\flanking\region\of\rabbit\ß-globin FT attenuator complement(2529..2653) FT /vntifkey="0" FT /label=attR2 FT RBS 2711..3291 FT /vntifkey="32" FT /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES) SQ Sequence 6273 BP; 1597 A; 1542 C; 1536 G; 1598 t; tcgagatatc tagcaacttt attatacaaa gttggcatta taaaaaagca ttgcttatca 60 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg gagctccatg 120 gtagcgttaa cgcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt 180 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 240 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat 300 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt 360 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt 420 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa 480 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac 540 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg 600 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc 660 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag 720 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc 780 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca 840 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa 900 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc 960 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt 1020 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt 1080 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca 1140 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag 1200 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 1260 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 1320 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 1380 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 1440 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 1500 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 1560 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 1620 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa 1680 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 1740 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 1800 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 1860 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 1920 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 1980 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 2040 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 2100 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt 2160 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca 2220 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc 2280 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga 2340 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct 2400 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct 2460 taagctcggg ccctgcagct ctagagctcg aattctacag gtcactaata ccatctaagt 2520 agttgattca tagtgactgc atatgttgtg ttttacagta ttatgtagtc tgttttttat 2580 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcaactttct 2640 tgtacaaagt ggttaaagga accaattcag tcgaggtcga cggtatcgat aagcttgata 2700 tcgaattccg cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct 2760 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg 2820 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt 2880 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg 2940 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac 3000 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg 3060 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct 3120 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg 3180 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag 3240 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca 3300 accatggtat ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc 3360 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 3420 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 3480 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 3540 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 3600 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 3660 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 3720 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 3780 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 3840 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 3900 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 3960 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 4020 tggacgagct gtacaagtac tcagatccac cggtcgccac cgaagacgcc aaaaacataa 4080 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata 4140 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg 4200 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga 4260 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat 4320 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca 4380 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg 4440 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa 4500 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg 4560 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata 4620 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg 4680 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg 4740 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg 4800 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat 4860 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg 4920 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta 4980 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg 5040 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag 5100 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat 5160 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag 5220 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga 5280 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg 5340 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc 5400 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag 5460 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg 5520 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa 5580 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg 5640 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga 5700 tcgccgtgta attctagagc ggccgcgatc tttttccctc tgccaaaaat tatggggaca 5760 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa 5820 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt 5880 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg 5940 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc 6000 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg 6060 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt 6120 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg 6180 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaagggcgaa 6240 ttctgcagat atccatcaca ctggcggccg cac 6273 //