LOCUS       pENTR1A-NLS-LacZ        5707 bp    DNA     circular UNA 22-NOV-2013
DEFINITION  LMBP 8208.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8208
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 22-NOV-2013
COMMENT     VNTDATE|677778478|
COMMENT     VNTNAME|pENTR1A-NLS-LacZ|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT            3558 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 3465 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 476 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NsiI" "atgcat" 6 0 2 4076 0 4342 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 6 1655 0 2108 0 2588 0 3314 
COMMENT            0 3508 0 4226 0 0 "")) 
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COMMENT            "complement(5292..5335)") 
COMMENT        #8=(CFSignal (CObList) "T2 terminator" 43 0 0 5630 5657 0 (CStringList) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 11/04/13 03:47" 1) 
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COMMENT                          "length: 5707 bp" 1) 
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COMMENT        #234=(CSampFrag "pENTR1A-NLS-LacZ" 1922 4342 "NsiI" 557 "HincII" 
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FEATURES             Location/Qualifiers
     rep_origin      5069..5394
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(5455..5498)
                     /vntifkey="43"
                     /label=T1\terminator
     terminator      5630..5657
                     /vntifkey="43"
                     /label=T2\terminator
     CDS             3800..4609
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     attenuator      1..96
                     /vntifkey="0"
                     /label=attL2
     attenuator      complement(3581..3676)
                     /vntifkey="0"
                     /label=attL1
     CDS             complement(375..3422)
                     /vntifkey="4"
                     /label=lacZ
     CDS             complement(3441..3461)
                     /vntifkey="4"
                     /label=SV40\large\T\NLS
BASE COUNT     1363 a      1472 c      1484 g      1388 t 
ORIGIN
        1 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gataagcaat 
       61 gctttcttat aatgccaact ttgtacaaga aagctgggtc tagagtcgag gccgagtttg 
      121 tcagaaagca gaccaaacag cggttggaat aatagcgaga acagagaaat agcggcaaaa 
      181 ataatacccg tatcactttt gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac 
      241 gggaagtagg ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag 
      301 tttgtgtttt ttaaatagta cataatggat ttccttacgc gaaatacggg cagacatggc 
      361 ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg accggcgctc 
      421 agctgtaatt ccgccgatac tgacgggctc caggagtcgt cgccaccaat ccccatatgg 
      481 aaaccgtcga tattcagcca tgtgccttct tccgcgtgca gcagatggcg atggctggtt 
      541 tccatcagtt gctgttgact gtagcggctg atgttgaact ggaagtcgcc gcgccactgg 
      601 tgtgggccat aattcaattc gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg 
      661 tacggggtat acatgtctga caatggcaga tcccagcggt caaaacaggc ggcagtaagg 
      721 cggtcgggat agttttcttg cggccctaat ccgagccagt ttacccgctc tgctacctgc 
      781 gccagctggc agttcaggcc aatccgcgcc ggatgcggtg tatcgctcgc cacttcaaca 
      841 tcaacggtaa tcgccatttg accactacca tcaatccggt aggttttccg gctgataaat 
      901 aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa tcagcaccgc atcagcaagt 
      961 gtatctgccg tgcactgcaa caacgctgct tcggcctggt aatggcccgc cgccttccag 
     1021 cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc 
     1081 agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca gttgtttttt atcgccaatc 
     1141 cacagctgtg aaagaaagcc tgactggcgg ttaaattgcc aacgcttatt acccagctcg 
     1201 atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg cgtgggacgc ggcggggagc 
     1261 gtcacactga ggttttccgc cagacgccac tgctgccagg cgctgatgtg cccggcttct 
     1321 gaccatccgg tcgcgttcgg ttgcactacg cgtactgtga gccagagttg cccggcgctc 
     1381 tccggctgcg gtagttcagg cagttcaatc aactgtttac cttgtggagc gacatccaga 
     1441 ggcacttcac cgcttgccag cggcttacca tccagcgcca ccatccagtg caggagctcg 
     1501 ttatcgctat gacggaacag gtattcgctg gtcacttcga tggtttgccc ggataaacgg 
     1561 aactggaaaa actgctgctg gtgttttgct tccgtcagcg ctggatgcgg cgtgcggtcg 
     1621 gcaaagacca gaccgttcat acagaactgg cgatcgttcg gcgtatcgcc aaaatcaccg 
     1681 ccgtaagccg accacgggtt gccgttttca tcatatttaa tcagcgactg atccacccag 
     1741 tcccagacga agccgccctg taaacgggga tactgacgaa acgcctgcca gtatttagcg 
     1801 aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc aaaggatcag cgggcgcgtc 
     1861 tctccaggta gcgaaagcca ttttttgatg gaccatttcg gcacagccgg gaagggctgg 
     1921 tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg tggccgtggt gtcggctccg 
     1981 ccgccttcat actgcaccgg gcgggaagga tcgacagatt tgatccagcg atacagcgcg 
     2041 tcgtgattag cgccgtggcc tgattcattc cccagcgacc agatgatcac actcgggtga 
     2101 ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca tcgccggtag ccagcgcgga 
     2161 tcatcggtca gcagattcat tggcaccatg ccgtgggttt caatattggc ttcatccacc 
     2221 acatacaggc cgtagcggtc gcacagcgtg taccacagcg gatggttcgg ataatgcgaa 
     2281 cagcgcacgg cgttaaagtt gttctgcttc atcagcagga tatcctgcac catcgtctgc 
     2341 tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc tcgaatcagc 
     2401 aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca 
     2461 tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac caccgcacga 
     2521 tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag acgtagtgtg 
     2581 acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg cgcggtgccg 
     2641 ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta gtcacgcaac 
     2701 tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc attaaagcga 
     2761 gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga gacgtcacgg 
     2821 aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc actccaacgc 
     2881 agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca 
     2941 gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca cagatgaaac 
     3001 gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca gctttcatca 
     3061 acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa cggcggattg 
     3121 accgtaatgg gataggttac gttggtgtag atgggcgcat cgtaaccgtg catctgccag 
     3181 tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca gctttccggc 
     3241 accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct gcgcaactgt 
     3301 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 
     3361 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 
     3421 acgggatccc ccatgctccc cactttgcgt ttcttcttgg gccccatggt ggatcctgag 
     3481 actggggaat tccccggatc cgtcgatcga ctctagatat ctcgagtgcg gccgcgaatt 
     3541 cggtaccgga tccagtcgac tgaattggtt cctttaaagc ctgctttttt gtacaaagtt 
     3601 ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa 
     3661 aataaaatca ttatttgcca tccagctgca gctctggccc gtgtctcaaa atctctgatg 
     3721 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa 
     3781 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa 
     3841 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc 
     3901 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca 
     3961 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac 
     4021 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt 
     4081 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc 
     4141 aggtgaaaat attgttgatg cgctggcagt gtccctgcgc cggttgcatt cgattcctgt 
     4201 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat 
     4261 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga 
     4321 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca 
     4381 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga 
     4441 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct 
     4501 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc 
     4561 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt 
     4621 taattggttg taacattatt cagattgggc cccgttccac tgagcgtcag accccgtaga 
     4681 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 
     4741 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 
     4801 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 
     4861 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 
     4921 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 
     4981 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 
     5041 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 
     5101 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 
     5161 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 
     5221 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 
     5281 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 
     5341 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgctagcat 
     5401 ggatctcggg gacgtctaac tactaagcga gagtagggaa ctgccaggca tcaaataaaa 
     5461 cgaaaggctc agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 
     5521 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga 
     5581 gggtggcggg caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc 
     5641 ctgacggatg gcctttttgc gtttctacaa actcttcctg ttagttagtt acttaagctc 
     5701 gggcccc 
//
