LOCUS       pENTR1A-OSKM      7277 bp    DNA     circular SYN 23-SEP-2009
DEFINITION  LMBP 8210.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     Terminator      complement(106..149)
                     /invitrogen="2580000"
                     /vntifkey="43"
                     /label="rrnB T1 transcription terminator"
                     /label="rrnB T1 transcription terminator"
     Terminator      complement(281..308)
                     /invitrogen="2590000"
                     /vntifkey="43"
                     /label="rrnB T2 transcription terminator"
                     /label="rrnB T2 transcription terminator"
     Misc._recombina 358..457
                     /invitrogen="2670000"
                     /vntifkey="86"
                     /label=attL1
     Misc._feature   509..1564
                     /vntifkey="21"
                     /label=Oct4
     Misc._binding   1565..1636
                     /vntifkey="20"
                     /label=T2A
     Misc._feature   1637..2593
                     /vntifkey="21"
                     /label=Sox2
     Misc._binding   2594..2665
                     /vntifkey="20"
                     /label=T2A
     Misc._feature   2666..4087
                     /vntifkey="21"
                     /label=Klf4
     Misc._binding   4088..4159
                     /vntifkey="20"
                     /label=F2A
     Misc._feature   4166..5485
                     /vntifkey="21"
                     /label=Myc
     Misc._recombina complement(5506..5605)
                     /invitrogen="2680000"
                     /vntifkey="86"
                     /label=attL2
     CDS             5728..6537
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     Replication_ori 6601..7274
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label="pUC origin"
                     /label="pUC origin"
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCA TGGATCTCGG
       61 GGACGTCTAA CTACTAAGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT
      121 CAGTCGGAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT
      181 AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGTGAAGC AACGGCCCGG AGGGTGGCGG
      241 GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAACTAAGC AGAAGGCCAT CCTGACGGAT
      301 GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTTAGTTAGT TACTTAAGCT CGGGCCCCAA
      361 ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC AACAAATTGA TAAGCAATGC
      421 TTTTTTATAA TGCCAACTTT GTACAAAAAA GCAGGCTTTA AAGGAACCAA TTCAGTCGAC
      481 TGGATCCGGT ACCGAATTCA GATCCACCAT GGCTGGACAC CTGGCTTCAG ACTTCGCCTT
      541 CTCACCCCCA CCAGGTGGGG GTGATGGGTC AGCAGGGCTG GAGCCGGGCT GGGTGGATCC
      601 TCGAACCTGG CTAAGCTTCC AAGGGCCTCC AGGTGGGCCT GGAATCGGAC CAGGCTCAGA
      661 GGTATTGGGG ATCTCCCCAT GTCCGCCCGC ATACGAGTTC TGCGGAGGGA TGGCATACTG
      721 TGGACCTCAG GTTGGACTGG GCCTAGTCCC CCAAGTTGGC GTGGAGACTT TGCAGCCTGA
      781 GGGCCAGGCA GGAGCACGAG TGGAAAGCAA CTCAGAGGGA ACCTCCTCTG AGCCCTGTGC
      841 CGACCGCCCC AATGCCGTGA AGTTGGAGAA GGTGGAACCA ACTCCCGAGG AGTCCCAGGA
      901 CATGAAAGCC CTGCAGAAGG AGCTAGAACA GTTTGCCAAG CTGCTGAAGC AGAAGAGGAT
      961 CACCTTGGGG TACACCCAGG CCGACGTGGG GCTCACCCTG GGCGTTCTCT TTGGAAAGGT
     1021 GTTCAGCCAG ACCACCATCT GTCGCTTCGA GGCCTTGCAG CTCAGCCTTA AGAACATGTG
     1081 TAAGCTGCGG CCCCTGCTGG AGAAGTGGGT GGAGGAAGCC GACAACAATG AGAACCTTCA
     1141 GGAGATATGC AAATCGGAGA CCCTGGTGCA GGCCCGGAAG AGAAAGCGAA CTAGCATTGA
     1201 GAACCGTGTG AGGTGGAGTC TGGAGACCAT GTTTCTGAAG TGCCCGAAGC CCTCCCTACA
     1261 GCAGATCACT CACATCGCCA ATCAGCTTGG GCTAGAGAAG GATGTGGTTC GAGTATGGTT
     1321 CTGTAACCGG CGCCAGAAGG GCAAAAGATC AAGTATTGAG TATTCCCAAC GAGAAGAGTA
     1381 TGAGGCTACA GGGACACCTT TCCCAGGGGG GGCTGTATCC TTTCCTCTGC CCCCAGGTCC
     1441 CCACTTTGGC ACCCCAGGCT ATGGAAGCCC CCACTTCACC ACACTCTACT CAGTCCCTTT
     1501 TCCTGAGGGC GAGGCCTTTC CCTCTGTTCC CGTCACTGCT CTGGGCTCTC CCATGCATTC
     1561 AAACGGCGGC GGGTCCGGAG GAGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTGGA
     1621 GGAGAATCCT GGCCCAATGT ATAACATGAT GGAGACGGAG CTGAAGCCGC CGGGCCCGCA
     1681 GCAAGCTTCG GGGGGCGGCG GCGGAGGAGG CAACGCCACG GCGGCGGCGA CCGGCGGCAA
     1741 CCAGAAGAAC AGCCCGGACC GCGTCAAGAG GCCCATGAAC GCCTTCATGG TATGGTCCCG
     1801 GGGGCAGCGG CGTAAGATGG CCCAGGAGAA CCCCAAGATG CACAACTCGG AGATCAGCAA
     1861 GCGCCTGGGC GCGGAGTGGA AACTTTTGTC CGAGACCGAG AAGCGGCCGT TCATCGACGA
     1921 GGCCAAGCGG CTGCGCGCTC TGCACATGAA GGAGCACCCG GATTATAAAT ACCGGCCGCG
     1981 GCGGAAAACC AAGACGCTCA TGAAGAAGGA TAAGTACACG CTTCCCGGAG GCTTGCTGGC
     2041 CCCCGGCGGG AACAGCATGG CGAGCGGGGT TGGGGTGGGC GCCGGCCTGG GTGCGGGCGT
     2101 GAACCAGCGC ATGGACAGCT ACGCGCACAT GAACGGCTGG AGCAACGGCA GCTACAGCAT
     2161 GATGCAGGAG CAGCTGGGCT ACCCGCAGCA CCCGGGCCTC AACGCTCACG GCGCGGCACA
     2221 GATGCAACCG ATGCACCGCT ACGACGTCAG CGCCCTGCAG TACAACTCCA TGACCAGCTC
     2281 GCAGACCTAC ATGAACGGCT CGCCCACCTA CAGCATGTCC TACTCGCAGC AGGGCACCCC
     2341 CGGTATGGCG CTGGGCTCCA TGGGCTCTGT GGTCAAGTCC GAGGCCAGCT CCAGCCCCCC
     2401 CGTGGTTACC TCTTCCTCCC ACTCCAGGGC GCCCTGCCAG GCCGGGGACC TCCGGGACAT
     2461 GATCAGCATG TACCTCCCCG GCGCCGAGGT GCCGGAGCCC GCTGCGCCCA GTAGACTGCA
     2521 CATGGCCCAG CACTACCAGA GCGGCCCGGT GCCCGGCACG GCCATTAACG GCACACTGCC
     2581 CCTGTCGCAC ATGGGCGGCG GGTCCGGAGG AGAGGGCAGA GGAAGTCTTC TAACATGCGG
     2641 TGACGTGGAG GAGAATCCTG GCCCAATGGC TGTCAGCGAC GCTCTGCTCC CGTCCTTCTC
     2701 CACGTTCGCG TCCGGCCCGG CGGGAAGGGA GAAGACACTG CGTCCAGCAG GTGCCCCGAC
     2761 TAACCGTTGG CGTGAGGAAC TCTCTCACAT GAAGCGACTT CCCCCACTTC CCGGCCGCCC
     2821 CTACGACCTG GCGGCGACGG TGGCCACAGA CCTGGAGAGT GGCGGAGCTG GTGCAGCTTG
     2881 CAGCAGTAAC AACCCGGCCC TCCTAGCCCG GAGGGAGACC GAGGAGTTCA ACGACCTCCT
     2941 GGACCTAGAC TTTATCCTTT CCAACTCGCT AACCCACCAG GAATCGGTGG CCGCCACCGT
     3001 GACCACCTCG GCGTCAGCTT CATCCTCGTC TTCCCCGGCG AGCAGCGGCC CTGCCAGCGC
     3061 GCCCTCCACC TGCAGCTTCA GCTATCCGAT CCGGGCCGGG GGTGACCCGG GCGTGGCTGC
     3121 CAGAAACACA GGTGGAGGGC TCCTCTACAG CCGAGAATCT GCGCCACCTC CCACGGCCCC
     3181 CTTCAACCTG GGGGACATCA ATGACGTGAG CCCCTCGGGC GGCTTCGTGG CTGAGCTCCT
     3241 GCGGCCGGAG TTGGACCCAG TATACATTCC GCCACAGCAG CCTCAGCCGC CAGGTGGGGG
     3301 GCTGATGGGC AAGTTTGTGC TGAAGGCGTC TCTGACCACC CCTGGCAGCG AGTACAGCAG
     3361 CCCTTCGGTC ATCAGTGTTA GCAAAGGAAG CCCAGACGGC AGCCACCCCG TGGTAGTGGC
     3421 GCCCTACAGC GGTGGCCCGC CGCGCATGTG CCCCAAGATT AAGCAAGAGG CGGTCCCGTC
     3481 CTGCACGGTC AGCCGGTCCC TAGAGGCCCA TTTGAGCGCT GGACCCCAGC TCAGCAACGG
     3541 CCACCGGCCC AACACACACG ACTTCCCCCT GGGGCGGCAG CTCCCCACCA GGACTACCCC
     3601 TACACTGAGT CCCGAGGAAC TGCTGAACAG CAGGGACTGT CACCCTGGCC TGCCTCTTCC
     3661 CCCAGGATTC CATCCCCATC CGGGGGCCAA CTACCCTCCT TTCCTGCCAG ACCAGATGCA
     3721 GTCACAAGTC CCCTCTCTCC ATTATCAAGA GCTCATGCCA CCGGGTTCCT GCCTGCCAGA
     3781 GGAGCCCAAG CCAAAGAGGG GAAGAAGGTC GTGGCCCCGG AAAAGAACAG CCACCCACAC
     3841 TTGTGACTAT GCAGGCTGTG GCAAAACCTA TACCAAGAGT TCTCATCTCA AGGCACACCT
     3901 GCGAACTCAC ACAGGCGAGA AACCTTACCA CTGTGACTGG GACGGCTGTG GGTGGAAATT
     3961 CGCCCGCTCC GATGAACTGA CCAGGCACTA CCGCAAACAC ACAGGGCACC GGCCCTTTCA
     4021 GTGCCAGAAG TGTGACAGGG CCTTTTCCAG GTCGGACCAC CTTGCCTTAC ACATGAAGAG
     4081 GCACTTTGGC AGCGGAAAAC AGCTGTTGAA TTTTGACCTT CTCAAGTTGG CGGGAGACGT
     4141 GGAGTCCAAC CCAGGGCCCG TCGAGATGCC CCTCAACGTG AACTTCACCA ACAGGAACTA
     4201 TGACCTCGAC TACGACTCCG TACAGCCCTA TTTCATCTGC GACGAGGAAG AGAATTTCTA
     4261 TCACCAGCAA CAGCAGAGCG AGCTGCAGCC GCCCGCGCCC AGTGAGGATA TCTGGAAGAA
     4321 ATTCGAGCTG CTTCCCACCC CGCCCCTGTC CCCGAGCCGC CGCTCCGGGC TCTGCTCTCC
     4381 ATCCTATGTT GCGGTCGCTA CGTCCTTCTC CCCAAGGGAA GACGATGACG GCGGCGGTGG
     4441 CAACTTCTCC ACCGCCGATC AGCTGGAGAT GATGACCGAG TTACTTGGAG GAGACATGGT
     4501 GAACCAGAGC TTCATCTGCG ATCCTGACGA CGAGACCTTC ATCAAGAACA TCATCATCCA
     4561 GGACTGTATG TGGAGCGGTT TCTCAGCCGC TGCCAAGCTG GTCTCGGAGA AGCTGGCCTC
     4621 CTACCAGGCT GCGCGCAAAG ACAGCACCAG CCTGAGCCCC GCCCGCGGGC ACAGCGTCTG
     4681 CTCCACCTCC AGCCTGTACC TGCAGGACCT CACCGCCGCC GCGTCCGAGT GCATTGACCC
     4741 CTCAGTGGTC TTTCCCTACC CGCTCAACGA CAGCAGCTCG CCCAAATCCT GTACCTCGTC
     4801 CGATTCCACG GCCTTCTCTC CTTCCTCGGA CTCGCTGCTG TCCTCCGAGT CCTCCCCACG
     4861 GGCCAGCCCT GAGCCCCTAG TGCTGCATGA GGAGACACCG CCCACCACCA GCAGCGACTC
     4921 TGAAGAAGAG CAAGAAGATG AGGAAGAAAT TGATGTGGTG TCTGTGGAGA AGAGGCAAAC
     4981 CCCTGCCAAG AGGTCGGAGT CGGGCTCATC TCCATCCCGA GGCCACAGCA AACCTCCGCA
     5041 CAGCCCACTG GTCCTCAAGA GGTGCCACGT CTCCACTCAC CAGCACAACT ACGCCGCACC
     5101 CCCCTCCACA AGGAAGGACT ATCCAGCTGC CAAGAGGGCC AAGTTGGACA GTGGCAGGGT
     5161 CCTGAAGCAG ATCAGCAACA ACCGCAAGTG CTCCAGCCCC AGGTCCTCAG ACACGGAGGA
     5221 AAACGACAAG AGGCGGACAC ACAACGTCTT GGAACGTCAG AGGAGGAACG AGCTGAAGCG
     5281 CAGCTTTTTT GCCCTGCGTG ACCAGATCCC TGAATTGGAA AACAACGAAA AGGCCCCCAA
     5341 GGTAGTGATC CTCAAAAAAG CCACCGCCTA CATCCTGTCC ATTCAAGCAG ACGAGCACAA
     5401 GCTCACCTCT GAAAAGGACT TATTGAGGAA ACGACGAGAA CAGTTGAAAC ACAAACTCGA
     5461 ACAGCTTCGA AACTCTGGTG CATGAGTCGA GATGCATCGA TCTAGACCCA GCTTTCTTGT
     5521 ACAAAGTTGG CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA
     5581 TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGCAGC TCTGGCCCGT GTCTCAAAAT
     5641 CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT GAACAATAAA ACTGTCTGCT
     5701 TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG
     5761 CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC
     5821 GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT
     5881 CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC
     5941 TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT
     6001 GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT
     6061 CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT CCCTGCGCCG GTTGCATTCG
     6121 ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA
     6181 TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG
     6241 CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC
     6301 GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT
     6361 TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG
     6421 AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT
     6481 GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA
     6541 GAATTGGTTA ATTGGTTGTA ACATTATTCA GATTGGGCCC CGTTCCACTG AGCGTCAGAC
     6601 CCGGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC
     6661 TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA
     6721 ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA
     6781 GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT
     6841 CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG
     6901 GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC
     6961 ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA
     7021 TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG
     7081 GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT
     7141 CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG
     7201 CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG
     7261 CCTTTTGCTC ACATGTT
//
