LOCUS       pEntry-L4-rtTA-IRES-Puro-pA-Ins/Ins-TRE-R1        6006 bp    DNA     circular     22-NOV-2013
DEFINITION  LMBP 8212.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8212
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 22-NOV-2013
            
COMMENT     VNTDATE|667053447|
COMMENT     VNTNAME|pEntry L4 rtTA-IRES-Puro-pA-Ins/Ins-TRE R1|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT            3590 0 4695 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PvuII" "cagctg" 4 0 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 4 7 0 974 0 4239 0 5260 0 
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FEATURES             Location/Qualifiers
     misc_feature    271..513
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     misc_feature    15..257
                     /vntifkey="21"
                     /label=chicken\ß-globin\5'HS4\insulator\core\sequence
     promoter        532..900
                     /vntifkey="29"
                     /label=hCMVT\promoter
     RBS             4501..5044
                     /vntifkey="32"
                     /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES)
     CDS             5045..5644
                     /vntifkey="4"
                     /label=puromycin\resistance
     attenuator      3303..3398
                     /vntifkey="0"
                     /label=attL4
     attenuator      complement(1030..1153)
                     /vntifkey="0"
                     /label=attR1
     CDS             1277..2086
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     terminator      5731..5955
                     /vntifkey="43"
                     /label=BGH\polyA
     CDS             3496..4500
                     /vntifkey="4"
                     /label=rtTA
     rep_origin      2546..2948
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      complement(3027..3070)
                     /vntifkey="43"
                     /label=T1\terminator
     terminator      3202..3229
                     /vntifkey="43"
                     /label=T2\terminator
BASE COUNT     1455 a      1581 c      1622 g      1348 t 
ORIGIN
        1 ggccgcggcg cgccgagctc acggggacag ccccccccca aagcccccag ggatgtaatt 
       61 acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc cggtccggcg 
      121 ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg ggaaggtggc 
      181 acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga cacctggggg 
      241 atacggggaa aaagcttggc caaaaaggcc gagctcacgg ggacagcccc cccccaaagc 
      301 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct 
      361 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg 
      421 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc 
      481 tgcagacacc tgggggatac ggggaaaaag cttttaatta agcggccgca ctcgagttta 
      541 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat 
      601 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg 
      661 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc 
      721 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga 
      781 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc 
      841 gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg 
      901 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg 
      961 atccagcctc cgcggccccg aattcggtac cggatccagt cgagatatct agaaagggcg 
     1021 aattcgaccc aagtttgtac aaaaaagttg aacgagaaac gtaaaatgat ataaatatca 
     1081 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata 
     1141 tgcagtcact atgatgcagt cactatggct ctggcccgtg tctcaaaatc tctgatgtta 
     1201 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag 
     1261 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc 
     1321 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg 
     1381 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg 
     1441 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga 
     1501 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact 
     1561 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg 
     1621 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg 
     1681 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa 
     1741 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca 
     1801 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg 
     1861 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt 
     1921 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg 
     1981 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga 
     2041 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa 
     2101 ttggttgtaa cattattcag attgggcccc gttccactga gcgtcagacc ccgtagaaaa 
     2161 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 
     2221 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 
     2281 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 
     2341 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 
     2401 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 
     2461 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 
     2521 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc 
     2581 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 
     2641 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 
     2701 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 
     2761 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 
     2821 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 
     2881 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 
     2941 ggaagagcgc ccaatacgca aaccgctagc atggatctcg gggacgtcta actactaagc 
     3001 gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcggaa gactgggcct 
     3061 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag 
     3121 cggatttgaa cgttgtgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa 
     3181 ctgccaggca tcaaactaag cagaaggcca tcctgacgga tggccttttt gcgtttctac 
     3241 aaactcttcc tgttagttag ttacttaagc tcgggcccgc gttaacgcta ccatggagct 
     3301 ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca 
     3361 atgctttttt ataatgccaa ctttgtatag aaaagttggc tccgaattcg cccttttaaa 
     3421 ggaaccaatt cagtcgaggt cgacggtatc gataagcttg atatcgaatt caccatgcca 
     3481 aagagaccca gaccctctag attagataaa agtaaagtga ttaacagcgc attagagctg 
     3541 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gcttggtgta 
     3601 gagcagccta cactgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc 
     3661 attgagatgt tagataggca ccatactcac ttttgccctt taaaagggga aagctggcaa 
     3721 gattttttac gcaataacgc taaaagtttt agatgtgctt tactaagtca tcgcaatgga 
     3781 gcaaaagtac attcagatac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa 
     3841 ttagcctttt tatgccaaca aggtttttca ctagagaacg cgttatatgc actcagcgct 
     3901 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa 
     3961 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta 
     4021 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga 
     4081 ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca gccgcgcgcg tacgaaaaac 
     4141 aattacgggt ctaccatcga gggcctgctc gatctcccgg acgacgacgc ccccgaagag 
     4201 gcggggctgg cggctccgcg cctgtccttt ctccccgcgg gacacacgcg cagactgtcg 
     4261 acggcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg cgaggacgtg 
     4321 gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg ggacggggat 
     4381 tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct ggatatggcc 
     4441 gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta cggtgggtag 
     4501 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata 
     4561 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt 
     4621 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa 
     4681 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag 
     4741 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca 
     4801 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc 
     4861 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat 
     4921 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta 
     4981 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga 
     5041 taatatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc 
     5101 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc 
     5161 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 
     5221 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 
     5281 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 
     5341 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa 
     5401 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 
     5461 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 
     5521 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 
     5581 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 
     5641 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc 
     5701 gatgatctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 
     5761 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 
     5821 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 
     5881 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 
     5941 cggtgggctc tatggcttct gaggcggaaa gaacctgcag cccgggggat ccactagttc 
     6001 tagagc 
//
