LOCUS       pGFP-N-GW               6448 bp    DNA     circular     28-OCT-2014
DEFINITION  LMBP 8367.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8367
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-OCT-2014
            
            
COMMENT     VNTDATE|664551722|
COMMENT     VNTNAME|pGFP-N-GW|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "NcoI" "NheI" "NotI" "PstI" "XhoI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT            5276 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 3292 0 3457 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 963 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1846 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 3432 0 0 "")) 
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COMMENT        #6=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 372 939 0 
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COMMENT        #7=(CFSignal (CObList) "attR1" 0 0 1 1719 1843 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 1851 1903 0 
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COMMENT        #9=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 1952 2611 0 
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COMMENT        #13=(CFSignal (CObList) "f1 ori" 33 0 1 3735 4190 0 (CStringList) 
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FEATURES             Location/Qualifiers
     rep_origin      39..365
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        372..939
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     attenuator      complement(1719..1843)
                     /vntifkey="0"
                     /label=attR1
     promoter        1851..1903
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             1952..2611
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             2953..3258
                     /vntifkey="4"
                     /label=ccdB
     attenuator      3299..3423
                     /vntifkey="0"
                     /label=attR2
     terminator      3585..3681
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      complement(3735..4190)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        4342..4623
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4580..4655
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             4715..5509
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      5700..5769
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     promoter        4191..4286
                     /vntifkey="30"
                     /label=ampicillin\resistance\promoter
     RBS             4287..4321
                     /vntifkey="32"
                     /label=5'\UTR\of\amp,\incl.\RBS
     terminator      complement(3585..3681)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             984..1700
                     /vntifkey="4"
                     /label=EGFP
BASE COUNT     1625 a      1631 c      1649 g      1543 t 
ORIGIN
        1 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 
       61 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 
      121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 
      181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 
      241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 
      301 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 
      361 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat 
      421 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 
      481 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 
      541 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 
      601 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 
      661 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 
      721 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 
      781 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 
      841 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 
      901 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc 
      961 cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg 
     1021 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 
     1081 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 
     1141 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 
     1201 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 
     1261 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 
     1321 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 
     1381 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 
     1441 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 
     1501 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 
     1561 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 
     1621 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 
     1681 gcatggacga gctgtacaag tccggactca gatcatcaac aagtttgtac aaaaaagctg 
     1741 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac 
     1801 agactacata atactgtaaa acacaacata tccagtcact atggcggccg cattaggcac 
     1861 cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc 
     1921 ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac 
     1981 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca 
     2041 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa 
     2101 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca 
     2161 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc 
     2221 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca 
     2281 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa 
     2341 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg 
     2401 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt 
     2461 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca 
     2521 ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca 
     2581 gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac taaaagccag 
     2641 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta 
     2701 tacccgaagt atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt gacagttgac 
     2761 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca 
     2821 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg 
     2881 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca 
     2941 gggactggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg 
     3001 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg 
     3061 gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc 
     3121 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc 
     3181 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg 
     3241 atgttctggg gaatataaat gtcaggctcc gttatacaca gccagtctgc aggtcgacca 
     3301 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta 
     3361 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt 
     3421 ggttgatgat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga 
     3481 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt 
     3541 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt 
     3601 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     3661 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     3721 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg 
     3781 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa 
     3841 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa 
     3901 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg 
     3961 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa 
     4021 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa 
     4081 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct 
     4141 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac 
     4201 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 
     4261 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 
     4321 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 
     4381 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 
     4441 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 
     4501 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 
     4561 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 
     4621 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 
     4681 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 
     4741 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 
     4801 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 
     4861 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 
     4921 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 
     4981 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 
     5041 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 
     5101 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 
     5161 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 
     5221 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 
     5281 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 
     5341 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 
     5401 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 
     5461 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 
     5521 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 
     5581 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 
     5641 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa 
     5701 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat 
     5761 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac 
     5821 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc 
     5881 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca 
     5941 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt 
     6001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 
     6061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     6121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     6181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     6241 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 
     6301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     6361 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     6421 cagcggtcgg gctgaacggg gggttcgt 
//
