LOCUS       pCR3-EGFPC-GW           7475 bp    DNA     circular     28-OCT-2014
DEFINITION  LMBP 8375.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 8375
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-OCT-2014
            
            
            
COMMENT     VNTDATE|664565548|
COMMENT     VNTNAME|pCR3-EGFPC-GW|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pCR3-EGFPC-GW" 0 7475 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
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COMMENT        (CWordArray) (CStringList "HindIII" "NcoI" "PstI" "XhoI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 665 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 4 386 0 1470 0 5016 0 5713 
COMMENT            0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 715 0 2295 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2452 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3176 0 0 "")) 
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COMMENT        #5=(CFSignal (CObList) "HSV-TK polyA" 43 0 1 4526 4595 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "SV40 ori" 33 0 1 5634 5709 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "SV40 early promoter" 29 0 1 5666 5947 0 
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COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6026 6886 0 
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COMMENT        #9=(CFSignal (CObList) "f1 ori" 33 0 0 7018 7473 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 1 4786 5574 0 
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COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 3482 3808 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "T7 promoter" 30 0 0 638 654 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "BGH polyA" 43 0 0 3234 3458 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 955 1614 0 
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FEATURES             Location/Qualifiers
     terminator      complement(4526..4595)
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     rep_origin      complement(5634..5709)
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(5666..5947)
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             complement(6026..6886)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      7018..7473
                     /vntifkey="33"
                     /label=f1\ori
     CDS             complement(4786..5574)
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     rep_origin      complement(3482..3808)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        638..654
                     /vntifkey="30"
                     /label=T7\promoter
     terminator      3234..3458
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        10..593
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     attenuator      complement(722..846)
                     /vntifkey="0"
                     /label=attR1
     promoter        854..906
                     /vntifkey="30"
                     /label=lacUV5\promoter
     CDS             955..1614
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             1956..2261
                     /vntifkey="4"
                     /label=ccdB
     attenuator      2302..2426
                     /vntifkey="0"
                     /label=attR2
     CDS             2458..3174
                     /vntifkey="4"
                     /label=EGFP
     promoter        complement(3192..3208)
                     /vntifkey="30"
                     /label=SP6\promoter
BASE COUNT     1845 a      1914 c      1900 g      1816 t 
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 
      181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 
      661 cccaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc 
      721 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 
      781 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 
      841 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat 
      901 gtgtggattt tgagttagga tccggcgaga ttttcaggag ctaaggaagc taaaatggag 
      961 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 
     1021 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 
     1081 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 
     1141 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg 
     1201 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 
     1261 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa 
     1321 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 
     1381 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 
     1441 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag 
     1501 gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc 
     1561 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct 
     1621 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt 
     1681 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc 
     1741 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt 
     1801 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga 
     1861 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa 
     1921 cggctctttt gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata 
     1981 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg 
     2041 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg 
     2101 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg 
     2161 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg 
     2221 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tccgttatac 
     2281 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta 
     2341 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct 
     2401 cgttcagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagcgtg 
     2461 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 
     2521 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 
     2581 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 
     2641 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 
     2701 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 
     2761 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 
     2821 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 
     2881 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 
     2941 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 
     3001 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 
     3061 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 
     3121 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaatctaga 
     3181 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc 
     3241 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 
     3301 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 
     3361 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 
     3421 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 
     3481 tggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
     3541 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
     3601 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
     3661 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
     3721 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
     3781 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
     3841 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     3901 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     3961 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     4021 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     4081 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     4141 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     4201 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     4261 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     4321 gtatatatga gtaacctgag gctatggcag ggcctgccgc cccgacgttg gctgcgagcc 
     4381 ctgggccttc acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt 
     4441 ggccccaatg gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc 
     4501 gtttatgaac aaacgaccca acaccgtgcg ttttattctg tctttttatt gccgtcatag 
     4561 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc ctagggtggg 
     4621 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa 
     4681 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca 
     4741 ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc 
     4801 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag 
     4861 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat 
     4921 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc 
     4981 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc 
     5041 gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc 
     5101 ttgatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg 
     5161 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc 
     5221 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc 
     5281 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc 
     5341 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc 
     5401 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag 
     5461 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag 
     5521 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa 
     5581 cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc 
     5641 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa 
     5701 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg 
     5761 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc 
     5821 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc 
     5881 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat 
     5941 tccacagctg gttctttccg cctcaggact cttccttttt caataaatca atctaaagta 
     6001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 
     6061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 
     6121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 
     6181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 
     6241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 
     6301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 
     6361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 
     6421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 
     6481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 
     6541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 
     6601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 
     6661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 
     6721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 
     6781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 
     6841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 
     6901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
     6961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
     7021 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     7081 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     7141 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 
     7201 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 
     7261 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     7321 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     7381 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     7441 cgaattttaa caaaatatta acgcttacaa tttac 
//
