ID pCR3-EGFPC-GW standard; circular DNA; ; 7475 BP. DE LMBP 8375. CC Accession number: LMBP 8375 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 28-OCT-2014 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT terminator complement(4526..4595) FT /vntifkey="43" FT /label=HSV-TK\polyA FT rep_origin complement(5634..5709) FT /vntifkey="33" FT /label=SV40\ori FT promoter complement(5666..5947) FT /vntifkey="29" FT /label=SV40\early\promoter FT CDS complement(6026..6886) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin 7018..7473 FT /vntifkey="33" FT /label=f1\ori FT CDS complement(4786..5574) FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT rep_origin complement(3482..3808) FT /vntifkey="33" FT /label=pMB1\ori FT promoter 638..654 FT /vntifkey="30" FT /label=T7\promoter FT terminator 3234..3458 FT /vntifkey="43" FT /label=BGH\polyA FT promoter 10..593 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT attenuator complement(722..846) FT /vntifkey="0" FT /label=attR1 FT promoter 854..906 FT /vntifkey="30" FT /label=lacUV5\promoter FT CDS 955..1614 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS 1956..2261 FT /vntifkey="4" FT /label=ccdB FT attenuator 2302..2426 FT /vntifkey="0" FT /label=attR2 FT CDS 2458..3174 FT /vntifkey="4" FT /label=EGFP FT promoter complement(3192..3208) FT /vntifkey="30" FT /label=SP6\promoter SQ Sequence 7475 BP; 1845 A; 1914 C; 1900 G; 1816 t; gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag 60 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct 120 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc 180 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg 240 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat 300 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 360 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 420 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 480 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 540 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc 600 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga 660 cccaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc 720 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 780 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 840 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat 900 gtgtggattt tgagttagga tccggcgaga ttttcaggag ctaaggaagc taaaatggag 960 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt 1020 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg 1080 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt 1140 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg 1200 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt 1260 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa 1320 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg 1380 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat 1440 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag 1500 gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc 1560 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct 1620 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt 1680 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc 1740 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt 1800 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga 1860 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa 1920 cggctctttt gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata 1980 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg 2040 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg 2100 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg 2160 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg 2220 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tccgttatac 2280 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta 2340 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct 2400 cgttcagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcgagcgtg 2460 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 2520 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 2580 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 2640 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 2700 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 2760 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 2820 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 2880 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 2940 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 3000 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 3060 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 3120 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaatctaga 3180 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc 3240 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 3300 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 3360 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 3420 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 3480 tggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3540 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3600 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3660 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3720 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3780 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 3840 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 3900 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 3960 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 4020 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 4080 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 4140 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 4200 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 4260 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4320 gtatatatga gtaacctgag gctatggcag ggcctgccgc cccgacgttg gctgcgagcc 4380 ctgggccttc acccgaactt ggggggtggg gtggggaaaa ggaagaaacg cgggcgtatt 4440 ggccccaatg gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc 4500 gtttatgaac aaacgaccca acaccgtgcg ttttattctg tctttttatt gccgtcatag 4560 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc ctagggtggg 4620 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa 4680 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca 4740 ggttgggcgt cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc 4800 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag 4860 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat 4920 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc 4980 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc 5040 gtcgggcatg ctcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc 5100 ttgatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg 5160 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc 5220 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc 5280 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc 5340 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc 5400 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag 5460 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag 5520 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa 5580 cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc 5640 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa 5700 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg 5760 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc 5820 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc 5880 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat 5940 tccacagctg gttctttccg cctcaggact cttccttttt caataaatca atctaaagta 6000 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 6060 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6120 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6180 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6240 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6300 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 6360 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6420 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6480 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6540 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6600 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 6660 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 6720 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 6780 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 6840 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 6900 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 6960 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7020 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 7080 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 7140 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 7200 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 7260 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 7320 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 7380 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 7440 cgaattttaa caaaatatta acgcttacaa tttac 7475 //