LOCUS       pLenti6(bla)-DYNLT1-tdTomato-V5        8857 bp    DNA     circular     11-OCT-2010
DEFINITION  LMBP 8392.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|567776931|
COMMENT     VNTDBDATE|567776931|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLenti6(bla)-DYNLT1-tdTomato-V5|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT             "pENTR3C-DYNLT1 no stop" "" 0 0 1 426 8432 8857 151 576 0 "" "" "" 
COMMENT             "" "" "" (CObList) (CTerm 2 0 151 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 2 1 576 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
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COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT        #4=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 34 0 6904 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 8468 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 8378 0 8843 0 0 "") 
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COMMENT        #9=(CRSite (CStringList) "taa" "taa" 1 0 122 452 0 1178 0 1494 0 1506 0 
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COMMENT        #10=(CRSite (CStringList) "tta" "tta" 1 0 131 291 0 1017 0 1542 0 1560 0 
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COMMENT             0 8673 0 8697 0 8720 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "tga" "tga" 1 0 154 15 0 50 0 113 0 185 0 191 
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COMMENT        #12=(CRSite (CStringList) "tca" "tca" 1 0 118 68 0 71 0 80 0 89 0 242 0 
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COMMENT             0 8311 0 8363 0 8379 0 8391 0 8565 0 8628 0 8631 0 8844 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "cta" "cta" 1 0 98 159 0 405 0 564 0 588 0 621 
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COMMENT             8378 0 8390 0 8500 0 8562 0 8754 0 8826 0 8843 0 0 "")) 
COMMENT       (CObList 
COMMENT        #15=(CFSignal (CObList) "attB1" 21 0 0 8424 8444 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "attB2" 21 0 1 8850 13 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "tdTomato" 4 0 0 46 1473 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "3 stops" 4 0 0 1543 1551 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"123000001\"") 1 1 1 1 "4248..4256") 
COMMENT        #19=(CFSignal (CObList) "SV40 early promoter" 29 0 0 1588 1897 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2290000\"") 1 1 1 1 
COMMENT             "4293..4602") 
COMMENT        #20=(CFSignal (CObList) "TATA box" 41 0 0 8292 8295 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"123000002\"") 1 1 1 1 "2309..2312") 
COMMENT        #21=(CFSignal (CObList) "splice acceptor" 38 0 0 7667 7667 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"123000014\"") 1 1 1 1 
COMMENT             "1684..1684") 
COMMENT        #22=(CFSignal (CObList) "splice acceptor" 38 0 0 7639 7639 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"123000013\"") 1 1 1 1 
COMMENT             "1656..1656") 
COMMENT        #23=(CFSignal (CObList) "splice donor" 38 0 0 6503 6503 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"123000012\"") 1 1 1 1 "520..520") 
COMMENT        #24=(CFSignal (CObList) "V5 epitope" 4 0 0 1492 1533 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1350000\"") 1 1 1 1 "4197..4238") 
COMMENT        #25=(CFSignal (CObList) "V5 reverse primer" 27 0 1 1501 1521 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2140000\"") 1 1 1 1 
COMMENT             "complement(4206..4226)") 
COMMENT        #26=(CFSignal (CObList) "SV40 pA" 25 0 0 2809 2939 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1840000\"") 1 1 1 1 "5514..5644") 
COMMENT        #27=(CFSignal (CObList) "RSV/5' LTR hybrid promoter" 29 0 0 5984 6393 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2280000\"") 1 1 1 1 
COMMENT             "1..410") 
COMMENT        #28=(CFSignal (CObList) "RSV promoter" 29 0 0 5984 6212 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2270000\"") 1 1 1 1 "1..229") 
COMMENT        #29=(CFSignal (CObList) "HIV-1 Rev response element" 31 0 0 7058 7291 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"2440000\"") 1 1 1 1 
COMMENT             "1075..1308") 
COMMENT        #30=(CFSignal (CObList) "pUC origin" 33 0 0 4903 5576 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2540000\"") 1 1 1 1 "7608..8281") 
COMMENT        #31=(CFSignal (CObList) "HIV-1 psi packaging signal" 20 0 0 6504 6548 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1780000\"") 1 1 1 1 
COMMENT             "521..565") 
COMMENT        #32=(CFSignal (CObList) "HIV-1 3' LTR" 19 0 0 2557 2737 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1430000\"") 1 1 1 1 "5262..5442") 
COMMENT        #33=(CFSignal (CObList) "HIV-1 5' LTR" 19 0 0 6213 6393 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1430000\"") 1 1 1 1 "230..410") 
COMMENT        #34=(CFSignal (CObList) "f1 origin" 33 0 0 3312 3767 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2520000\"") 1 1 1 1 "6017..6472") 
COMMENT        #35=(CFSignal (CObList) "EM7 promoter" 30 0 0 1951 2018 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2340000\"") 1 1 1 1 "4656..4723") 
COMMENT        #36=(CFSignal (CObList) "deltaU3" 19 0 0 2503 2556 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1410000\"") 1 1 1 1 "5208..5261") 
COMMENT        #37=(CFSignal (CObList) "CMV promoter" 29 0 0 7792 8374 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2170000\"") 1 1 1 1 "1809..2391") 
COMMENT        #38=(CFSignal (CObList) "CMV forward primer" 27 0 0 8257 8277 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1930000\"") 1 1 1 1 
COMMENT             "2274..2294") 
COMMENT        #39=(CFSignal (CObList) "Bsd(R)" 4 0 0 2019 2417 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1050000\"") 1 1 1 1 "4724..5122") 
COMMENT        #40=(CFSignal (CObList) "bla promoter" 30 0 0 3799 3897 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"2330000\"") 1 1 1 1 "6504..6602") 
COMMENT        #41=(CFSignal (CObList) "Amp(R)" 4 0 0 3898 4758 1 (CStringList) 
COMMENT             (CStringList "/invitrogen=\"1020000\"") 1 1 1 1 "6603..7463") 
COMMENT        #42=(CFSignal (CObList) "deltaU3/3' LTR" 19 0 0 2504 2737 1 
COMMENT             (CStringList) (CStringList "/invitrogen=\"1390000\"") 1 1 1 1 
COMMENT             "5209..5442") 
COMMENT        #43=(CFSignal (CObList) "DYNLT1 no stop" 4 0 0 8492 8830 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #44=(CFSignal (CObList) "Phe" 4 0 0 8855 8857 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "669..671") 
COMMENT        #45=(CFSignal (CObList) "Ser" 4 0 0 8447 8449 1 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "166..168")) (CObList) (CObList) (CObList) 
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COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) 
COMMENT                    "pLenti6(bla)-DYNLT1-tdTomato-V5" 2) 5 "" 0 4) 
COMMENT              #48=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #49=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA 'pLenti6(bla)-DYNLT1-tdTomato-V5'" 1) 
COMMENT                     #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "attB1-attB2" 1) 
COMMENT                     #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #52=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 10/11/10 11:48" 1) 
COMMENT                     #53=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/11/10 11:48" 1) 
COMMENT                     #54=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8857 bp" 1) 
COMMENT                     #55=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
COMMENT                     #56=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #57=(CFolderPar 
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COMMENT                   "Standard Fields") 
COMMENT              #58=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) 
COMMENT                   "Annotations") 
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COMMENT                   (CGroupPar (CParagraph 11 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #60=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Mix Entry Clone pENTR3C-DYNLT1 no stop and Destination Vector pLenti6/tdTomato-V5-DEST in LR buffer and TE buffers" 
COMMENT                          1) 
COMMENT                     #61=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Thaw, vortex and add LR Clonase enzyme (LR Clonase Plus enzyme for multi-site Gateway). Incubate >= 1 hr. at 25\260." 
COMMENT                          1) 
COMMENT                     #62=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Add Proteinase K for 10 min at 37\260." 1) 
COMMENT                     #63=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Transform into DH5a E. coli cells (One Shot TOP10 for multi-site Gateway) or any non-F' episome bearing strain." 
COMMENT                          1) 
COMMENT                     #64=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Select Expression Clones on agar plates with antibiotics ampicillin or blasticidin ." 
COMMENT                          1) 
COMMENT                     #65=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Express in appropriate host cells." 1))) 
COMMENT                   "Design Description") 
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COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 8384 " 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 8475 " 1))))) "BamHI: 2 sites") 1 
COMMENT                          5 "GGATCC" "CCTAGG") 
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COMMENT                                  (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 19 " 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 8419 " 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #3# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 8841 " 1))))) "EcoRV: 3 sites") 3 
COMMENT                          3 "GATATC" "CTATAG") 
COMMENT                     #76=(CRSFolderPar 
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FEATURES             Location/Qualifiers
     misc_feature    8424..8444
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(8850..13)
                     /vntifkey="21"
                     /label=attB2
     CDS             46..1473
                     /vntifkey="4"
                     /label=tdTomato
     CDS             1543..1551
                     /invitrogen="123000001"
                     /vntifkey="4"
                     /label=3\stops
     promoter        1588..1897
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     TATA_signal     8292..8295
                     /invitrogen="123000002"
                     /vntifkey="41"
                     /label=TATA\box
     splicing_signal 7667..7667
                     /invitrogen="123000014"
                     /vntifkey="38"
                     /label=splice\acceptor
     splicing_signal 7639..7639
                     /invitrogen="123000013"
                     /vntifkey="38"
                     /label=splice\acceptor
     splicing_signal 6503..6503
                     /invitrogen="123000012"
                     /vntifkey="38"
                     /label=splice\donor
     CDS             1492..1533
                     /invitrogen="1350000"
                     /vntifkey="4"
                     /label=V5\epitope
     primer          complement(1501..1521)
                     /invitrogen="2140000"
                     /vntifkey="27"
                     /label=V5\reverse\primer
     polyA_signal    2809..2939
                     /invitrogen="1840000"
                     /vntifkey="25"
                     /label=SV40\pA
     promoter        5984..6393
                     /invitrogen="2280000"
                     /vntifkey="29"
                     /label=RSV/5'\LTR\hybrid\promoter
     promoter        5984..6212
                     /invitrogen="2270000"
                     /vntifkey="29"
                     /label=RSV\promoter
     protein_bind    7058..7291
                     /invitrogen="2440000"
                     /vntifkey="31"
                     /label=HIV-1\Rev\response\element
     rep_origin      4903..5576
                     /invitrogen="2540000"
                     /vntifkey="33"
                     /label=pUC\origin
     misc_binding    6504..6548
                     /invitrogen="1780000"
                     /vntifkey="20"
                     /label=HIV-1\psi\packaging\signal
     LTR             2557..2737
                     /invitrogen="1430000"
                     /vntifkey="19"
                     /label=HIV-1\3'\LTR
     LTR             6213..6393
                     /invitrogen="1430000"
                     /vntifkey="19"
                     /label=HIV-1\5'\LTR
     rep_origin      3312..3767
                     /invitrogen="2520000"
                     /vntifkey="33"
                     /label=f1\origin
     promoter        1951..2018
                     /invitrogen="2340000"
                     /vntifkey="30"
                     /label=EM7\promoter
     LTR             2503..2556
                     /invitrogen="1410000"
                     /vntifkey="19"
                     /label=deltaU3
     promoter        7792..8374
                     /invitrogen="2170000"
                     /vntifkey="29"
                     /label=CMV\promoter
     primer          8257..8277
                     /invitrogen="1930000"
                     /vntifkey="27"
                     /label=CMV\forward\primer
     CDS             2019..2417
                     /invitrogen="1050000"
                     /vntifkey="4"
                     /label=Bsd(R)
     promoter        3799..3897
                     /invitrogen="2330000"
                     /vntifkey="30"
                     /label=bla\promoter
     CDS             3898..4758
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=Amp(R)
     LTR             2504..2737
                     /invitrogen="1390000"
                     /vntifkey="19"
                     /label=deltaU3/3'\LTR
     CDS             8492..8830
                     /vntifkey="4"
                     /label=DYNLT1\no\stop
     CDS             8855..8857
                     /vntifkey="4"
                     /label=Phe
     CDS             8447..8449
                     /vntifkey="4"
                     /label=Ser
BASE COUNT     2303 a      2216 c      2298 g      2040 t 
ORIGIN
        1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtatggt gagcaagggc 
       61 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc 
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 
      181 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag 
      241 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag 
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg 
      361 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg 
      421 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag 
      481 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc 
      541 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag 
      601 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac 
      661 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc 
      721 ctggggcatg gcaccggcag caccggcagc ggcagctccg gcaccgcctc ctccgaggac 
      781 aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg 
      841 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag 
      901 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc 
      961 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac 
     1021 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc 
     1081 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg 
     1141 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc 
     1201 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac 
     1261 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg 
     1321 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc 
     1381 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac 
     1441 ctgttcctgt acggcatgga cgagctgtac aaggagggcc cgcggttcga aggtaagcct 
     1501 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agtttggaat 
     1561 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc 
     1621 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 
     1681 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 
     1741 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 
     1801 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc 
     1861 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct 
     1921 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata 
     1981 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct ttgtctcaag 
     2041 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag 
     2101 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg 
     2161 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg 
     2221 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga 
     2281 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag 
     2341 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt 
     2401 atgtgtggga gggctaagca caattcgagc tcggtacctt taagaccaat gacttacaag 
     2461 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac 
     2521 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc 
     2581 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg 
     2641 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc 
     2701 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt 
     2761 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt 
     2821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     2881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg 
     2941 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 
     3001 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 
     3061 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa 
     3121 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga 
     3181 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 
     3241 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 
     3301 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     3361 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     3421 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     3481 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
     3541 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     3601 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     3661 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     3721 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt 
     3781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 
     3841 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg 
     3901 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt 
     3961 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga 
     4021 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 
     4081 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt 
     4141 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 
     4201 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 
     4261 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 
     4321 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 
     4381 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 
     4441 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 
     4501 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 
     4561 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 
     4621 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 
     4681 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 
     4741 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 
     4801 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     4861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     4921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     4981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     5041 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 
     5101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     5161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     5221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     5281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     5341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     5401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     5461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     5521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 
     5581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 
     5641 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 
     5701 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 
     5761 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 
     5821 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 
     5881 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca 
     5941 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa 
     6001 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga 
     6061 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa 
     6121 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata 
     6181 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag 
     6241 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 
     6301 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 
     6361 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc 
     6421 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc 
     6481 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa 
     6541 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga 
     6601 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc 
     6661 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg 
     6721 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc 
     6781 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac 
     6841 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca 
     6901 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat 
     6961 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga 
     7021 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct 
     7081 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac 
     7141 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac 
     7201 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg 
     7261 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca 
     7321 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt 
     7381 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac 
     7441 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat 
     7501 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa 
     7561 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt 
     7621 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa 
     7681 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag 
     7741 acagatccat tcgattagtg aacggatctc gacggtatcg ataagcttgg gagttccgcg 
     7801 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 
     7861 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 
     7921 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 
     7981 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 
     8041 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 
     8101 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat 
     8161 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 
     8221 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac 
     8281 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc 
     8341 atccacgctg ttttgacctc catagaagac accgactcta gaggatccac tagtccagtg 
     8401 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctctt taaaggaacc 
     8461 aattcagtcg actggatccg gtaccgaatt catggaagac taccaggctg cggaggagac 
     8521 tgcttttgtt gttgatgaag tgagcaacat tgtaaaagag gctatagaaa gcgcaattgg 
     8581 tggtaacgct tatcaacaca gcaaagtgaa ccagtggacc acaaatgtag tagaacaaac 
     8641 tttaagccaa ctcaccaagc tgggaaaacc atttaaatac atcgtgacct gtgtaattat 
     8701 gcagaagaat ggagctggat tacacacagc aagttcctgc ttctgggaca gctctactga 
     8761 cgggagctgc actgtgcgat gggagaataa gaccatgtac tgcatcgtca gtgccttcgg 
     8821 actgtctatt gcctcgagat atctagaccc agctttc 
//
