LOCUS       pLenti6-mBeclin1-C-Frag-122-V5-puro      8224 bp    DNA     circular SYN 10-FEB-2012
DEFINITION  LMBP 8560.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8560
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
DBSOURCE    GenBank
FEATURES             Location/Qualifiers
     Misc._recombina complement(join(8218..8224,1..14))
                     /label=attB2
     CDS             68..109
                     /invitrogen="1350000"
                     /note="Oriinal location: 8371..8412"
                     /label="V5 epitope"
     CDS             119..127
                     /invitrogen="123000001"
                     /note="Oriinal location: 8422..8430"
                     /label="3 stops"
     Promoter        164..473
                     /invitrogen="2290000"
                     /note="Oriinal location: 8467..8776"
                     /label="SV40 early promoter"
     CDS             533..1135
                     /invitrogen="1050000"
                     /note="Oriinal location: 1..603"
                     /label=Puro(R)
     LTR             1226..1279
                     /invitrogen="1410000"
                     /note="Oriinal location: 694..747"
                     /label=deltaU3
     LTR             1227..1460
                     /invitrogen="1390000"
                     /note="Oriinal location: 695..928"
                     /label="deltaU3/3' LTR"
     LTR             1280..1460
                     /invitrogen="1430000"
                     /note="Oriinal location: 748..928"
                     /label="HIV-1 3' LTR"
     PolyA_signal    1532..1662
                     /invitrogen="1840000"
                     /note="Oriinal location: 1000..1130"
                     /label="SV40 pA"
     Replication_ori 2035..2490
                     /invitrogen="2520000"
                     /note="Oriinal location: 1503..1958"
                     /label="f1 origin"
     Promoter        2522..2620
                     /invitrogen="2330000"
                     /note="Oriinal location: 1990..2088"
                     /label="bla promoter"
     CDS             2621..3481
                     /invitrogen="1020000"
                     /note="Oriinal location: 2089..2949"
                     /label=Amp(R)
     Replication_ori 3626..4299
                     /invitrogen="2540000"
                     /note="Oriinal location: 3094..3767"
                     /label="pUC origin"
     Source          4706^4707
                     /invitrogen="765"
                     /note="Oriinal location: 4175..4174"
                     /label=Source_1
     Promoter        4707..4935
                     /invitrogen="2270000"
                     /note="Oriinal location: 4175..4403"
                     /label="RSV promoter"
     Promoter        4707..5116
                     /invitrogen="2280000"
                     /note="Oriinal location: 4175..4584"
                     /label="RSV/5' LTR hybrid promoter"
     LTR             4936..5116
                     /invitrogen="1430000"
                     /note="Oriinal location: 4404..4584"
                     /label="HIV-1 5' LTR"
     Misc._feature   5226
                     /invitrogen="123000012"
                     /note="Oriinal location: 4694..4694"
                     /label="splice donor"
     Misc._binding   5227..5271
                     /invitrogen="1780000"
                     /note="Oriinal location: 4695..4739"
                     /label="HIV-1 psi packaging signal"
     Protein_binding 5781..6014
                     /invitrogen="2440000"
                     /note="Oriinal location: 5249..5482"
                     /label="HIV-1 Rev response element"
     Misc._feature   6362
                     /invitrogen="123000013"
                     /note="Oriinal location: 5830..5830"
                     /label="splice acceptor"
     Misc._feature   6390
                     /invitrogen="123000014"
                     /note="Oriinal location: 5858..5858"
                     /label="splice acceptor"
     Promoter        6515..7097
                     /invitrogen="2170000"
                     /note="Oriinal location: 5983..6565"
                     /label="CMV promoter"
     TATA_box        7015..7018
                     /invitrogen="123000002"
                     /note="Oriinal location: 6483..6486"
                     /label="TATA box"
     Misc._recombina 7147..7167
                     /label=attB1
     Source          join(<8202..>8224,<7154..>7209)
                     /invitrogen="563"
                     /label=Source_1
     Vector          join(8202..>8224,<7154..7209)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     CDS             7218..8198
                     /origin="mBeclin + stop + primers (1.384bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1369^1370, 1.358bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="mBeclin-C- Frag_122 - stop"
ORIGIN
        1 CTTGTACAAA GTGGTTGATA TCCAGCACAG TGGCGGCCGC TCGAGTCTAG AGGGCCCGCG
       61 GTTCGAAGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGC GTACCGGTTA
      121 GTAATGAGTT TGGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA
      181 GGCTCCCCAG GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG
      241 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC
      301 AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC
      361 CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC
      421 TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
      481 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGA CCATGGCCAC
      541 CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC GTCCCCCGGG CCGTACGCAC
      601 CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC ACCGTCGACC CGGACCGCCA
      661 CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG CGCGTCGGGC TCGACATCGG
      721 CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC TGGACCACGC CGGAGAGCGT
      781 CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CTCGCGCATG GCCGAGTTGA GCGGTTCCCG
      841 GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG CACCGGCCCA AGGAGCCCGC
      901 GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG GGCAAGGGTC TGGGCAGCGC
      961 CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCTGGG GTGCCCGCCT TCCTGGAGAC
     1021 CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC TTCACCGTCA CCGCCGACGT
     1081 CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC AAGCCCGGTG CCTGAGCTAA
     1141 GCACAATTCG AGCTCGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG TAGATCTTAG
     1201 CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC GAAGACAAGA
     1261 TCTGCTTTTT GCTTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC
     1321 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT
     1381 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC
     1441 AGTGTGGAAA ATCTCTAGCA GTAGTAGTTC ATGTCATCTT ATTATTCAGT ATTTATAACT
     1501 TGCAAAGAAA TGAATATCAG AGAGTGAGAG GAACTTGTTT ATTGCAGCTT ATAATGGTTA
     1561 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG
     1621 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGGCTCTAGC TATCCCGCCC
     1681 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC
     1741 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG
     1801 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGGACGTACC CAATTCGCCC TATAGTGAGT
     1861 CGTATTACGC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG
     1921 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG
     1981 AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGGACGCGC
     2041 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC
     2101 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG
     2161 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT
     2221 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC
     2281 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT
     2341 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA
     2401 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA
     2461 ATTTTAACAA AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA ATGTGCGCGG
     2521 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA
     2581 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG
     2641 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC
     2701 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT
     2761 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT
     2821 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA
     2881 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC
     2941 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT
     3001 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC
     3061 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT
     3121 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC
     3181 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA
     3241 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG
     3301 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT
     3361 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC
     3421 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA
     3481 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT
     3541 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA
     3601 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC
     3661 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT
     3721 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC
     3781 GCAGATACCA AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC
     3841 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG
     3901 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG
     3961 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA
     4021 ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC
     4081 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG
     4141 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG
     4201 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT
     4261 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC
     4321 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG
     4381 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC
     4441 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG
     4501 GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA
     4561 GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT
     4621 TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG
     4681 GGAACAAAAG CTGGAGCTGC AAGCTTAATG TAGTCTTATG CAATACTCTT GTAGTCTTGC
     4741 AACATGGTAA CGATGAGTTA GCAACATGCC TTACAAGGAG AGAAAAAGCA CCGTGCATGC
     4801 CGATTGGTGG AAGTAAGGTG GTACGATCGT GCCTTATTAG GAAGGCAACA GACGGGTCTG
     4861 ACATGGATTG GACGAACCAC TGAATTGCCG CATTGCAGAG ATATTGTATT TAAGTGCCTA
     4921 GCTCGATACA TAAACGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC
     4981 TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG
     5041 TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG
     5101 TGGAAAATCT CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG AAACCAGAGG
     5161 AGCTCTCTCG ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC GAGGGGCGGC
     5221 GACTGGTGAG TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA GATGGGTGCG
     5281 AGAGCGTCAG TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT CGGTTAAGGC
     5341 CAGGGGGAAA GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG GAGCTAGAAC
     5401 GATTCGCAGT TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA ATACTGGGAC
     5461 AGCTACAACC ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT AATACAGTAG
     5521 CAACCCTCTA TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA GCTTTAGACA
     5581 AGATAGAGGA AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGCGGCC GCTGATCTTC
     5641 AGACCTGGAG GAGGAGATAT GAGGGACAAT TGGAGAAGTG AATTATATAA ATATAAAGTA
     5701 GTAAAAATTG AACCATTAGG AGTAGCACCC ACCAAGGCAA AGAGAAGAGT GGTGCAGAGA
     5761 GAAAAAAGAG CAGTGGGAAT AGGAGCTTTG TTCCTTGGGT TCTTGGGAGC AGCAGGAAGC
     5821 ACTATGGGCG CAGCGTCAAT GACGCTGACG GTACAGGCCA GACAATTATT GTCTGGTATA
     5881 GTGCAGCAGC AGAACAATTT GCTGAGGGCT ATTGAGGCGC AACAGCATCT GTTGCAACTC
     5941 ACAGTCTGGG GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG ATACCTAAAG
     6001 GATCAACAGC TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC CACTGCTGTG
     6061 CCTTGGAATG CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA CACGACCTGG
     6121 ATGGAGTGGG ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT AATTGAAGAA
     6181 TCGCAAAACC AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA ATGGGCAAGT
     6241 TTGTGGAATT GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT CATAATGATA
     6301 GTAGGAGGCT TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT GAATAGAGTT
     6361 AGGCAGGGAT ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG GGGACCCGAC
     6421 AGGCCCGAAG GAATAGAAGA AGAAGGTGGA GAGAGAGACA GAGACAGATC CATTCGATTA
     6481 GTGAACGGAT CTCGACGGTA TCGATAAGCT TGGGAGTTCC GCGTTACATA ACTTACGGTA
     6541 AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT
     6601 GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG
     6661 TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC
     6721 GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT
     6781 CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG
     6841 CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC
     6901 ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT
     6961 AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
     7021 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC
     7081 CTCCATAGAA GACACCGACT CTAGAGGATC CACTAGTCCA GTGTGGTGGA ATTCTGCAGA
     7141 TATCAACAAG TTTGTACAAA AAAGCAGGCT CTTTAAAGGA ACCAATTCAG TCGACTGGAT
     7201 CCGGTACCGA ATTCACCATG ATCATGTCGG GCCAGACCGA TGTGGATCAC CCGCTGTGTG
     7261 AGGAATGCAC AGACACTCTT TTAGACCAGC TGGACACTCA GCTCAATGTC ACTGAGAATG
     7321 AATGTCAGAA CTACAAACGC TGTTTGGAGA TCCTAGAGCA GATGAATGAG GATGACAGTG
     7381 AGCAGCTGCA GAGGGAGCTG AAGGAGCTGG CCTTGGAGGA GGAGAGGCTG ATCCAGGAGC
     7441 TGGAAGATGT GGAAAAGAAC CGCAAGGTGG TGGCAGAGAA CCTGGAGAAG GTCCAGGCTG
     7501 AGGCGGAGAG ATTGGACCAG GAGGAAGCTC AGTACCAGCG GGAGTATAGT GAGTTTAAAA
     7561 GGCAGCAGCT GGAGTTGGAT GACGAACTCA AGAGTGTGGA GAACCAGGTG CGCTACGCCC
     7621 AGATCCAGCT GGACAAGCTC AAGAAAACCA ATGTCTTCAA TGCCACCTTC CACATCTGGC
     7681 ACAGCGGACA GTTTGGCACA ATCAATAATT TCAGACTGGG TCGCTTGCCC AGTGTTCCTG
     7741 TGGAGTGGAA TGAAATCAAT GCTGCCTGGG GCCAGACAGT GCTGCTGCTC CATGCTTTGG
     7801 CCAATAAGAT GGGTCTGAAG TTTCAGAGGT ACCGACTTGT TCCCTATGGA AATCATTCCT
     7861 ATCTGGAGTC TCTGACAGAC AAATCTAAGG AGTTGCCGTT ATACTGTTCT GGGGGTTTGC
     7921 GGTTTTTCTG GGACAACAAG TTTGACCATG CAATGGTAGC TTTTCTGGAC TGTGTGCAGC
     7981 AGTTCAAAGA AGAGGTGGAA AAAGGAGAGA CTCGATTTTG TCTTCCGTAC AGGATGGACG
     8041 TGGAGAAAGG CAAGATTGAA GACACTGGAG GCAGTGGCGG CTCCTATTCC ATCAAAACCC
     8101 AGTTTAACTC GGAGGAGCAG TGGACAAAAG CGCTCAAGTT CATGCTGACC AATCTCAAGT
     8161 GGGGTCTTGC CTGGGTGTCC TCACAGTTCT ATAACAAGCA CTCGAGATAT CTAGACCCAG
     8221 CTTT
//
