LOCUS       pLenti6-mBeclin1-tdTomato-V5-puro       10012 bp    DNA     circular SYN 18-MAR-2015
DEFINITION  LMBP 8563.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8563
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)

COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|728661644|
COMMENT     VNTDBDATE|728732896|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLenti6-mBeclin1-tdTomato-V5-puro|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTREPLTYPE|Virus
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pLenti6-mBeclin1-tdTomato-V5-puro" 0 10012 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 9347 9346 1 10012 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 2973 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 9555 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 601 0 1825 0 2551 0 9998 
COMMENT            0 0 "") #3=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 9972 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 5 31 0 1064 0 2997 0 
COMMENT            5668 0 8186 0 0 "")) 
COMMENT       (CObList 
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COMMENT             "Fragment: from EcoRI cut at 11^12 to XhoI cut at" 
COMMENT             "1369^1370, 1.358bp           Orientation: original" 
COMMENT             "Plus strand 5':   no change           Plus strand 3':   no" 
COMMENT             "change           Minus strand 5':  no change" 
COMMENT             "Minus strand 3':  no change\"") 1 1 1 1 "65..1408") 
COMMENT        #6=(CFSignal (CObList) "attB2" 0 0 1 1425 1449 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1428..1448)") 
COMMENT        #7=(CFSignal (CObList) "tdTomato" 4 0 0 1481 2908 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1481..2908") 
COMMENT        #8=(CFSignal (CObList) "V5 epitope" 4 0 0 2927 2968 0 (CStringList) 
COMMENT            (CStringList "/invitrogen=\"1350000\"") 1 1 1 1 "2927..2968") 
COMMENT        #9=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3001 3283 0 
COMMENT            (CStringList) (CStringList "/invitrogen=\"2290000\"") 1 1 1 1 
COMMENT            "3023..3332") 
COMMENT        #10=(CFSignal (CObList) "puromycin resistance" 4 0 0 3392 3994 0 
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COMMENT             "3392..3994") 
COMMENT        #11=(CFSignal (CObList) "ampicillin resistance" 4 0 0 5480 6340 0 
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COMMENT             "5480..6340") 
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COMMENT             "8086..8130") 
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COMMENT             "8640..8873") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 0 6881 7283 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 0 7459 7496 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "lac promoter" 30 0 0 7375 7458 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "T3 promoter" 30 0 0 7522 7538 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "RSV/HIV-1 5' LTR" 29 0 0 7647 7975 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 9372 9888 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "SV40 ori" 33 0 0 3240 3315 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "HIV-1 cPPT" 21 0 0 4069 4084 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #22=(CFSignal (CObList) "RSV/HIV-1 dU3/3' LTR" 43 0 0 4085 4319 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #23=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4391 4465 0 
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COMMENT        #24=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4391 4465 0 
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COMMENT        #25=(CFSignal (CObList) "SV40 ori" 33 0 0 4601 4676 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #26=(CFSignal (CObList) "T7 promoter" 30 0 1 4710 4726 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #27=(CFSignal (CObList) "f1 ori" 33 0 0 4894 5349 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
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COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
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COMMENT                          "DNA Plasmid Phage Virus 'pLenti6-mBeclin1-tdTomato-V5-puro'" 
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COMMENT                          "Collection N\260.: 8563." 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/17/15 02:00" 1) 
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COMMENT                          "Last Modified: 03/18/15 09:48" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 10012 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 17-MAR-2015" 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/pENTR3C\">pENTR3C</a>" 
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COMMENT                          "Sequence Source: Unknown (unknown)." 1) 
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COMMENT                   "Restriction/Methylation Map") 
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COMMENT        #358=(CSampFrag "pLenti6-mBeclin1-tdTomato-V5-puro" 2671 
COMMENT              2997 "XmnI" 5668 "XmnI" (LOGPEN 0 0 0) 4.44273e-316 7.26286e-283) 
COMMENT        #359=(CSampFrag "pLenti6-mBeclin1-tdTomato-V5-puro" 2518 
COMMENT              5668 "XmnI" 8186 "XmnI" (LOGPEN 0 0 0) 4.24399e-314 5.10138e-283) 
COMMENT        #360=(CSampFrag "pLenti6-mBeclin1-tdTomato-V5-puro" 1933 
COMMENT              1064 "XmnI" 2997 "XmnI" (LOGPEN 0 0 0) 4.82156e+199 1.75004) 
COMMENT        #361=(CSampFrag "pLenti6-mBeclin1-tdTomato-V5-puro" 1857 
COMMENT              8186 "XmnI" 31 "XmnI" (LOGPEN 0 0 0) 1.32772e-300 1.09471e-300) 
COMMENT        #362=(CSampFrag "pLenti6-mBeclin1-tdTomato-V5-puro" 1033 
COMMENT              31 "XmnI" 1064 "XmnI" (LOGPEN 0 0 0) 1.37913e-316 1.02894e-314))))
FEATURES             Location/Qualifiers
     Source          join(<1412..>1434,<1..>56)
                     /invitrogen="563"
                     /vntifkey="21"
                     /label=Source_1
                     /note="Unknown feature type:Source"
     Misc._recombina join(10006..10012,1..14)
                     /vntifkey="21"
                     /label=attB1
                     /note="Unknown feature type:Misc._recombina"
     Vector          join(1412..>1434,<1..56)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Unknown feature type:Vector"
     CDS             65..1408
                     /origin="mBeclin + stop + primers (1.384bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1369^1370, 1.358bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /vntifkey="4"
                     /label=mBeclin\-\stop
     attenuator      complement(1425..1449)
                     /vntifkey="0"
                     /label=attB2
     CDS             1481..2908
                     /vntifkey="4"
                     /label=tdTomato
                     /note="Oriinal location: 4176..5603"
     CDS             2927..2968
                     /invitrogen="1350000"
                     /vntifkey="4"
                     /label=V5\epitope
                     /note="Oriinal location: 5622..5663"
     promoter        3001..3283
                     /invitrogen="2290000"
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             3392..3994
                     /invitrogen="1050000"
                     /vntifkey="4"
                     /label=puromycin\resistance
     CDS             5480..6340
                     /invitrogen="1020000"
                     /vntifkey="4"
                     /label=ampicillin\resistance
     Misc._binding   8086..8130
                     /invitrogen="1780000"
                     /vntifkey="21"
                     /label=HIV-1\psi\packaging\signal
                     /note="Unknown feature type:Misc._binding  Oriinal location: 521..565"
     Protein_binding 8640..8873
                     /invitrogen="2440000"
                     /vntifkey="21"
                     /label=HIV-1\Rev\response\element
                     /note="Unknown feature type:Protein_binding  Oriinal location: 1075..1308"
     rep_origin      6881..7283
                     /vntifkey="33"
                     /label=pMB1\ori
     RBS             7459..7496
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        7375..7458
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        7522..7538
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        7647..7975
                     /vntifkey="29"
                     /label=RSV/HIV-1\5'\LTR
     promoter        9372..9888
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      3240..3315
                     /vntifkey="33"
                     /label=SV40\ori
     misc_feature    4069..4084
                     /vntifkey="21"
                     /label=HIV-1\cPPT
     terminator      4085..4319
                     /vntifkey="43"
                     /label=RSV/HIV-1\dU3/3'\LTR
     terminator      4391..4465
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4391..4465)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      4601..4676
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(4710..4726)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      4894..5349
                     /vntifkey="33"
                     /label=f1\ori
     attenuator      10005..17
                     /vntifkey="0"
                     /label=attB1
BASE COUNT     2537 a      2575 c      2685 g      2215 t 
ORIGIN
        1 gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg actggatccg gtaccgaatt 
       61 caccatggag gggtctaagg cgtccagcag caccatgcag gtgagcttcg tgtgccagcg 
      121 ctgtagccag cctctgaaac tggacacgag cttcaagatc ctggaccggg tcaccatcca 
      181 ggaactcaca gctccattac ttaccacagc ccaggcgaaa ccaggagaga cccaggagga 
      241 agaggctaac tcaggagagg agccatttat tgaaactcgc caggatggtg tctctcgaag 
      301 attcatcccc ccagccagga tgatgtctac agaaagtgct aatagcttca ctctgatcgg 
      361 ggaggcatct gatggtggca ccatggagaa cctcagccgg agactcaagg tcactggaga 
      421 cctgtttgac atcatgtcgg gccagaccga tgtggatcac ccgctgtgtg aggaatgcac 
      481 agacactctt ttagaccagc tggacactca gctcaatgtc actgagaatg aatgtcagaa 
      541 ctacaaacgc tgtttggaga tcctagagca gatgaatgag gatgacagtg agcagctgca 
      601 gagggagctg aaggagctgg ccttggagga ggagaggctg atccaggagc tggaagatgt 
      661 ggaaaagaac cgcaaggtgg tggcagagaa cctggagaag gtccaggctg aggcggagag 
      721 attggaccag gaggaagctc agtaccagcg ggagtatagt gagtttaaaa ggcagcagct 
      781 ggagttggat gacgaactca agagtgtgga gaaccaggtg cgctacgccc agatccagct 
      841 ggacaagctc aagaaaacca atgtcttcaa tgccaccttc cacatctggc acagcggaca 
      901 gtttggcaca atcaataatt tcagactggg tcgcttgccc agtgttcctg tggagtggaa 
      961 tgaaatcaat gctgcctggg gccagacagt gctgctgctc catgctttgg ccaataagat 
     1021 gggtctgaag tttcagaggt accgacttgt tccctatgga aatcattcct atctggagtc 
     1081 tctgacagac aaatctaagg agttgccgtt atactgttct gggggtttgc ggtttttctg 
     1141 ggacaacaag tttgaccatg caatggtagc ttttctggac tgtgtgcagc agttcaaaga 
     1201 agaggtggaa aaaggagaga ctcgattttg tcttccgtac aggatggacg tggagaaagg 
     1261 caagattgaa gacactggag gcagtggcgg ctcctattcc atcaaaaccc agtttaactc 
     1321 ggaggagcag tggacaaaag cgctcaagtt catgctgacc aatctcaagt ggggtcttgc 
     1381 ctgggtgtcc tcacagttct ataacaagca ctcgagatat ctagacccag ctttcttgta 
     1441 caaagtggtt gatatccagc acagtggcgg ccgctcgagt atggtgagca agggcgagga 
     1501 ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga 
     1561 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct 
     1621 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat 
     1681 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc 
     1741 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac 
     1801 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg 
     1861 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc 
     1921 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa 
     1981 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc 
     2041 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga 
     2101 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg 
     2161 gcatggcacc ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa 
     2221 catggccgtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg 
     2281 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 
     2341 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca 
     2401 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa 
     2461 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct 
     2521 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat 
     2581 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga 
     2641 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc 
     2701 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa 
     2761 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca 
     2821 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt 
     2881 cctgtacggc atggacgagc tgtacaagga gggcccgcgg ttcgaaggta agcctatccc 
     2941 taaccctctc ctcggtctcg attctacgcg taccggttag taatgagttt ggaattaatt 
     3001 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag 
     3061 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 
     3121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 
     3181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     3241 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 
     3301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 
     3361 atccattttc ggatctgatc agcacgtgac catggccacc gagtacaagc ccacggtgcg 
     3421 cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga 
     3481 ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct 
     3541 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 
     3601 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 
     3661 cgagatcggc tcgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 
     3721 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 
     3781 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 
     3841 ggcggccgag cgcgctgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 
     3901 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 
     3961 cacctggtgc atgacccgca agcccggtgc ctgagctaag cacaattcga gctcggtacc 
     4021 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 
     4081 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg 
     4141 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 
     4201 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 
     4261 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 
     4321 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 
     4381 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 
     4441 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 
     4501 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc 
     4561 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 
     4621 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 
     4681 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg 
     4741 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 
     4801 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 
     4861 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg 
     4921 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg 
     4981 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc 
     5041 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa 
     5101 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc 
     5161 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 
     5221 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt 
     5281 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc 
     5341 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 
     5401 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 
     5461 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 
     5521 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 
     5581 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 
     5641 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 
     5701 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 
     5761 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 
     5821 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 
     5881 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 
     5941 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 
     6001 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 
     6061 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 
     6121 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 
     6181 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 
     6241 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 
     6301 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 
     6361 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 
     6421 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 
     6481 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 
     6541 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 
     6601 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 
     6661 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 
     6721 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 
     6781 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 
     6841 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 
     6901 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 
     6961 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 
     7021 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 
     7081 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 
     7141 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 
     7201 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 
     7261 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 
     7321 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 
     7381 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 
     7441 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 
     7501 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca 
     7561 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 
     7621 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 
     7681 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 
     7741 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 
     7801 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 
     7861 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 
     7921 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 
     7981 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 
     8041 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 
     8101 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 
     8161 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 
     8221 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 
     8281 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 
     8341 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 
     8401 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 
     8461 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 
     8521 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 
     8581 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 
     8641 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 
     8701 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 
     8761 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 
     8821 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 
     8881 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 
     8941 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 
     9001 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 
     9061 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 
     9121 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 
     9181 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 
     9241 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 
     9301 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat 
     9361 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg 
     9421 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata 
     9481 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 
     9541 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 
     9601 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 
     9661 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 
     9721 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 
     9781 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 
     9841 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 
     9901 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc 
     9961 tagaggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt tt 
//
