LOCUS       pENTR3C-mBeclin1-N-Frag-147+stop        2736 bp    DNA     circular SYN 19-MAR-1980
DEFINITION  LMBP 8589.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8589
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Unknown Unclassified.
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|pENTR3C-mBeclin1-N-Frag-147+stop|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 57 0 350 0 952 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 485 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") 
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COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 504 0 822 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1078 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) 
COMMENT       (CObList 
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COMMENT             "complement(106..149)") 
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COMMENT        #13=(CFSignal (CObList) "Beclin1_N-term_147 + stop" 4 0 0 505 948 0 
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COMMENT              "1369^1370, 1.358bp           Orientation: original" 
COMMENT              "Plus strand 5':   no change           Plus strand 3':   no" 
COMMENT              "change           Minus strand 5':  no change" 
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COMMENT             "complement(965..1064)") 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" 
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COMMENT                          "Modification Date in the Original DB: 09-FEB-2012" 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/pENTR3C\">pENTR3C</a>" 
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FEATURES             Location/Qualifiers
     Source          join(<952..2736,1..>496)
                     /invitrogen="563"
                     /vntifkey="21"
                     /label=Source_1
                     /note="Unknown feature type:Source"
     Vector          join(952..2736,1..496)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Unknown feature type:Vector"
     Terminator      complement(106..149)
                     /invitrogen="2580000"
                     /vntifkey="21"
                     /label=rrnB\T1\transcription\terminator
                     /note="Unknown feature type:Terminator"
     Terminator      complement(281..308)
                     /invitrogen="2590000"
                     /vntifkey="21"
                     /label=rrnB\T2\transcription\terminator
                     /note="Unknown feature type:Terminator"
     Misc._recombina 358..457
                     /invitrogen="2670000"
                     /vntifkey="21"
                     /label=attL1
                     /note="Unknown feature type:Misc._recombina"
     CDS             505..948
                     /origin="mBeclin + stop + primers (1.384bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1369^1370, 1.358bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /vntifkey="4"
                     /label=Beclin1_N-term_147\+\stop
     Misc._recombina complement(965..1064)
                     /invitrogen="2680000"
                     /vntifkey="21"
                     /label=attL2
                     /note="Unknown feature type:Misc._recombina"
     CDS             1187..1996
                     /invitrogen="1210000"
                     /vntifkey="4"
                     /label=Kan(R)
     Replication_ori 2060..2733
                     /invitrogen="2540000"
                     /vntifkey="21"
                     /label=pUC\origin
                     /note="Unknown feature type:Replication_ori"
BASE COUNT      710 a       634 c       681 g       711 t 
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg 
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct 
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg 
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat 
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa 
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc 
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctctt taaaggaacc aattcagtcg 
      481 actggatccg gtaccgaatt caccatggag gggtctaagg cgtccagcag caccatgcag 
      541 gtgagcttcg tgtgccagcg ctgtagccag cctctgaaac tggacacgag cttcaagatc 
      601 ctggaccggg tcaccatcca ggaactcaca gctccattac ttaccacagc ccaggcgaaa 
      661 ccaggagaga cccaggagga agaggctaac tcaggagagg agccatttat tgaaactcgc 
      721 caggatggtg tctctcgaag attcatcccc ccagccagga tgatgtctac agaaagtgct 
      781 aatagcttca ctctgatcgg ggaggcatct gatggtggca ccatggagaa cctcagccgg 
      841 agactcaagg tcactggaga cctgtttgac atcatgtcgg gccagaccga tgtggatcac 
      901 ccgctgtgtg aggaatgcac agacactctt ttagaccagc tggactgaca ctcgagatat 
      961 ctagacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt 
     1021 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgcagct 
     1081 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg 
     1141 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca 
     1201 acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 
     1261 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc 
     1321 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 
     1381 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 
     1441 tatccgtact cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt 
     1501 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 
     1561 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt 
     1621 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga 
     1681 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc 
     1741 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 
     1801 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 
     1861 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 
     1921 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct 
     1981 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cattattcag attgggcccc 
     2041 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 
     2101 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 
     2161 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 
     2221 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 
     2281 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 
     2341 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 
     2401 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 
     2461 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 
     2521 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 
     2581 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 
     2641 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 
     2701 gttcctggcc ttttgctggc cttttgctca catgtt 
//
