LOCUS       pLenti6-mBeclin1-N-Frag-131-puro      7615 bp    DNA     circular SYN 10-FEB-2012
DEFINITION  LMBP 8595.
SOURCE
  ORGANISM
COMMENT     Accession number: LMBP 8595
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
DBSOURCE    GenBank
FEATURES             Location/Qualifiers
     Misc._recombina complement(join(7609..7615,1..14))
                     /label=attB2
     Misc._feature   58..82
                     /label="FLAG tag"
     CDS             95..103
                     /invitrogen="123000001"
                     /note="Oriinal location: 8422..8430"
                     /label="3 stops"
     Promoter        140..449
                     /invitrogen="2290000"
                     /note="Oriinal location: 8467..8776"
                     /label="SV40 early promoter"
     CDS             509..1111
                     /invitrogen="1050000"
                     /note="Oriinal location: 1..603"
                     /label=Puro(R)
     LTR             1202..1255
                     /invitrogen="1410000"
                     /note="Oriinal location: 694..747"
                     /label=deltaU3
     LTR             1203..1436
                     /invitrogen="1390000"
                     /note="Oriinal location: 695..928"
                     /label="deltaU3/3' LTR"
     LTR             1256..1436
                     /invitrogen="1430000"
                     /note="Oriinal location: 748..928"
                     /label="HIV-1 3' LTR"
     PolyA_signal    1508..1638
                     /invitrogen="1840000"
                     /note="Oriinal location: 1000..1130"
                     /label="SV40 pA"
     Replication_ori 2011..2466
                     /invitrogen="2520000"
                     /note="Oriinal location: 1503..1958"
                     /label="f1 origin"
     Promoter        2498..2596
                     /invitrogen="2330000"
                     /note="Oriinal location: 1990..2088"
                     /label="bla promoter"
     CDS             2597..3457
                     /invitrogen="1020000"
                     /note="Oriinal location: 2089..2949"
                     /label=Amp(R)
     Replication_ori 3602..4275
                     /invitrogen="2540000"
                     /note="Oriinal location: 3094..3767"
                     /label="pUC origin"
     Promoter        4683..4911
                     /invitrogen="2270000"
                     /note="Oriinal location: 4175..4403"
                     /label="RSV promoter"
     Promoter        4683..5092
                     /invitrogen="2280000"
                     /note="Oriinal location: 4175..4584"
                     /label="RSV/5' LTR hybrid promoter"
     Source          4682^4683
                     /invitrogen="765"
                     /note="Oriinal location: 4175..4174"
                     /label=Source_1
     LTR             4912..5092
                     /invitrogen="1430000"
                     /note="Oriinal location: 4404..4584"
                     /label="HIV-1 5' LTR"
     Misc._feature   5202
                     /invitrogen="123000012"
                     /note="Oriinal location: 4694..4694"
                     /label="splice donor"
     Misc._binding   5203..5247
                     /invitrogen="1780000"
                     /note="Oriinal location: 4695..4739"
                     /label="HIV-1 psi packaging signal"
     Protein_binding 5757..5990
                     /invitrogen="2440000"
                     /note="Oriinal location: 5249..5482"
                     /label="HIV-1 Rev response element"
     Misc._feature   6338
                     /invitrogen="123000013"
                     /note="Oriinal location: 5830..5830"
                     /label="splice acceptor"
     Misc._feature   6366
                     /invitrogen="123000014"
                     /note="Oriinal location: 5858..5858"
                     /label="splice acceptor"
     Promoter        6491..7073
                     /invitrogen="2170000"
                     /note="Oriinal location: 5983..6565"
                     /label="CMV promoter"
     TATA_box        6991..6994
                     /invitrogen="123000002"
                     /note="Oriinal location: 6483..6486"
                     /label="TATA box"
     Misc._recombina 7123..7143
                     /label=attB1
     Source          join(<7593..>7615,<7130..>7185)
                     /invitrogen="563"
                     /label=Source_1
     Vector          join(7593..>7615,<7130..7185)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     Restriction_sit join(7179..7184,7183^7184)
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="Number_of_matches: 2"
                     /note="cut_0_on_positive_strand: 494^495"
                     /note="cut_0_on_negative_strand: 490^491"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     CDS             7194..7589
                     /origin="mBeclin + stop + primers (1.384bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1369^1370, 1.358bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label=mBeclin1-N-term_131_nostop
ORIGIN
        1 CTTGTACAAA GTGGTTGATA TCCAGCACAG TGGCGGCCGC TCGAGTCTAG AGGGCCCGGA
       61 CTACAAGGAT GACGATGACA AAACGCGTAC CGGTTAGTAA TGAGTTTGGA ATTAATTCTG
      121 TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGGCAG GCAGAAGTAT
      181 GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC
      241 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC
      301 TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT
      361 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA
      421 GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC
      481 CATTTTCGGA TCTGATCAGC ACGTGACCAT GGCCACCGAG TACAAGCCCA CGGTGCGCCT
      541 CGCCACCCGC GACGACGTCC CCCGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA
      601 CCCCGCCACG CGCCACACCG TCGACCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA
      661 AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG
      721 CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA
      781 GATCGGCTCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA
      841 AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT
      901 CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC
      961 GGCCGAGCGC GCTGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT
     1021 CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC
     1081 CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AGCTAAGCAC AATTCGAGCT CGGTACCTTT
     1141 AAGACCAATG ACTTACAAGG CAGCTGTAGA TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG
     1201 ACTGGAAGGG CTAATTCACT CCCAACGAAG ACAAGATCTG CTTTTTGCTT GTACTGGGTC
     1261 TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA ACCCACTGCT
     1321 TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA
     1381 CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT CTAGCAGTAG
     1441 TAGTTCATGT CATCTTATTA TTCAGTATTT ATAACTTGCA AAGAAATGAA TATCAGAGAG
     1501 TGAGAGGAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA
     1561 TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA
     1621 TGTATCTTAT CATGTCTGGC TCTAGCTATC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA
     1681 ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA
     1741 GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA
     1801 GGCCTAGGGA CGTACCCAAT TCGCCCTATA GTGAGTCGTA TTACGCGCGC TCACTGGCCG
     1861 TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG
     1921 CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC
     1981 AACAGTTGCG CAGCCTGAAT GGCGAATGGG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG
     2041 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC
     2101 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA
     2161 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC
     2221 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT
     2281 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA
     2341 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT
     2401 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGCTTA
     2461 CAATTTAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA
     2521 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA
     2581 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC
     2641 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA
     2701 AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT
     2761 TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG
     2821 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA
     2881 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT
     2941 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT
     3001 ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA
     3061 TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA
     3121 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA
     3181 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC
     3241 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC
     3301 CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG
     3361 TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT
     3421 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA
     3481 TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT
     3541 TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA
     3601 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG
     3661 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC
     3721 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT
     3781 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC
     3841 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT
     3901 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG
     3961 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT
     4021 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG
     4081 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG
     4141 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
     4201 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
     4261 GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC
     4321 CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT
     4381 GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT
     4441 TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG AGCGCAACGC
     4501 AATTAATGTG AGTTAGCTCA CTCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
     4561 TCGTATGTTG TGTGGAATTG TGAGCGGATA ACAATTTCAC ACAGGAAACA GCTATGACCA
     4621 TGATTACGCC AAGCGCGCAA TTAACCCTCA CTAAAGGGAA CAAAAGCTGG AGCTGCAAGC
     4681 TTAATGTAGT CTTATGCAAT ACTCTTGTAG TCTTGCAACA TGGTAACGAT GAGTTAGCAA
     4741 CATGCCTTAC AAGGAGAGAA AAAGCACCGT GCATGCCGAT TGGTGGAAGT AAGGTGGTAC
     4801 GATCGTGCCT TATTAGGAAG GCAACAGACG GGTCTGACAT GGATTGGACG AACCACTGAA
     4861 TTGCCGCATT GCAGAGATAT TGTATTTAAG TGCCTAGCTC GATACATAAA CGGGTCTCTC
     4921 TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG
     4981 CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT
     5041 GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTGGCGCC
     5101 CGAACAGGGA CTTGAAAGCG AAAGGGAAAC CAGAGGAGCT CTCTCGACGC AGGACTCGGC
     5161 TTGCTGAAGC GCGCACGGCA AGAGGCGAGG GGCGGCGACT GGTGAGTACG CCAAAAATTT
     5221 TGACTAGCGG AGGCTAGAAG GAGAGAGATG GGTGCGAGAG CGTCAGTATT AAGCGGGGGA
     5281 GAATTAGATC GCGATGGGAA AAAATTCGGT TAAGGCCAGG GGGAAAGAAA AAATATAAAT
     5341 TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT CGCAGTTAAT CCTGGCCTGT
     5401 TAGAAACATC AGAAGGCTGT AGACAAATAC TGGGACAGCT ACAACCATCC CTTCAGACAG
     5461 GATCAGAAGA ACTTAGATCA TTATATAATA CAGTAGCAAC CCTCTATTGT GTGCATCAAA
     5521 GGATAGAGAT AAAAGACACC AAGGAAGCTT TAGACAAGAT AGAGGAAGAG CAAAACAAAA
     5581 GTAAGACCAC CGCACAGCAA GCGGCCGCTG ATCTTCAGAC CTGGAGGAGG AGATATGAGG
     5641 GACAATTGGA GAAGTGAATT ATATAAATAT AAAGTAGTAA AAATTGAACC ATTAGGAGTA
     5701 GCACCCACCA AGGCAAAGAG AAGAGTGGTG CAGAGAGAAA AAAGAGCAGT GGGAATAGGA
     5761 GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA GGAAGCACTA TGGGCGCAGC GTCAATGACG
     5821 CTGACGGTAC AGGCCAGACA ATTATTGTCT GGTATAGTGC AGCAGCAGAA CAATTTGCTG
     5881 AGGGCTATTG AGGCGCAACA GCATCTGTTG CAACTCACAG TCTGGGGCAT CAAGCAGCTC
     5941 CAGGCAAGAA TCCTGGCTGT GGAAAGATAC CTAAAGGATC AACAGCTCCT GGGGATTTGG
     6001 GGTTGCTCTG GAAAACTCAT TTGCACCACT GCTGTGCCTT GGAATGCTAG TTGGAGTAAT
     6061 AAATCTCTGG AACAGATTTG GAATCACACG ACCTGGATGG AGTGGGACAG AGAAATTAAC
     6121 AATTACACAA GCTTAATACA CTCCTTAATT GAAGAATCGC AAAACCAGCA AGAAAAGAAT
     6181 GAACAAGAAT TATTGGAATT AGATAAATGG GCAAGTTTGT GGAATTGGTT TAACATAACA
     6241 AATTGGCTGT GGTATATAAA ATTATTCATA ATGATAGTAG GAGGCTTGGT AGGTTTAAGA
     6301 ATAGTTTTTG CTGTACTTTC TATAGTGAAT AGAGTTAGGC AGGGATATTC ACCATTATCG
     6361 TTTCAGACCC ACCTCCCAAC CCCGAGGGGA CCCGACAGGC CCGAAGGAAT AGAAGAAGAA
     6421 GGTGGAGAGA GAGACAGAGA CAGATCCATT CGATTAGTGA ACGGATCTCG ACGGTATCGA
     6481 TAAGCTTGGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
     6541 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
     6601 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
     6661 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
     6721 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
     6781 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG
     6841 CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT
     6901 TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA
     6961 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT
     7021 CAGATCGCCT GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA CCGACTCTAG
     7081 AGGATCCACT AGTCCAGTGT GGTGGAATTC TGCAGATATC AACAAGTTTG TACAAAAAAG
     7141 CAGGCTCTTT AAAGGAACCA ATTCAGTCGA CTGGATCCGG TACCGAATTC ACCATGGAGG
     7201 GGTCTAAGGC GTCCAGCAGC ACCATGCAGG TGAGCTTCGT GTGCCAGCGC TGTAGCCAGC
     7261 CTCTGAAACT GGACACGAGC TTCAAGATCC TGGACCGGGT CACCATCCAG GAACTCACAG
     7321 CTCCATTACT TACCACAGCC CAGGCGAAAC CAGGAGAGAC CCAGGAGGAA GAGGCTAACT
     7381 CAGGAGAGGA GCCATTTATT GAAACTCGCC AGGATGGTGT CTCTCGAAGA TTCATCCCCC
     7441 CAGCCAGGAT GATGTCTACA GAAAGTGCTA ATAGCTTCAC TCTGATCGGG GAGGCATCTG
     7501 ATGGTGGCAC CATGGAGAAC CTCAGCCGGA GACTCAAGGT CACTGGAGAC CTGTTTGACA
     7561 TCATGTCGGG CCAGACCGAT GTGGATTGAC ACTCGAGATA TCTAGACCCA GCTTT
//
