LOCUS       pLenti6(puro)-FLAG-dest-strep-hMLKL(FL)-K230M-stop      8665 bp    DNA     circular SYN 09-FEB-2012
DEFINITION  LMBP 8800.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     Misc._recombina join(8659..8665,1..14)
                     /label=attB1
     Source          join(<1514..>1536,<1..>56)
                     /label=Source_1
     Vector          join(1514..>1536,<1..56)
                     /origin="pENTR3C (2.723bp circular)          Target
                     vector: from XhoI cut at 938^939 to EcoRI cut at 496^497,
                     2.281bp          Orientation: original          Plus
                     strand 5':   no change          Plus strand 3':   no
                     change          Minus strand 5':  no change          Minus
                     strand 3':  no change"
     Misc._signal    65..100
                     /origin="strep + hMLKL( + primer) (1.486bp linear)
                     Fragment: from EcoRI cut at 11^12 to XhoI cut at
                     1471^1472, 1.460bp           Orientation: original
                     Plus strand 5':   no change           Plus strand 3':   no
                     change           Minus strand 5':  no change
                     Minus strand 3':  no change"
                     /label="strep tag"
     CDS             101..1510
                     /label="hMLKL(FL) - K230M - stop"
     Misc._recombina complement(1530..1550)
                     /label=attB2
     Misc._feature   1594..1618
                     /label="FLAG tag"
     CDS             1631..1639
                     /label="3 stops"
     Promoter        1676..1985
                     /label="SV40 early promoter"
     CDS             2045..2647
                     /label=Puro(R)
     LTR             2738..2791
                     /label=deltaU3
     LTR             2739..2972
                     /label="deltaU3/3' LTR"
     LTR             2792..2972
                     /label="HIV-1 3' LTR"
     PolyA_signal    3044..3174
                     /label="SV40 pA"
     Replication_ori 3547..4002
                     /label="f1 origin"
     Promoter        4034..4132
                     /label="bla promoter"
     CDS             4133..4993
                     /label=Amp(R)
     Replication_ori 5138..5811
                     /label="pUC origin"
     Promoter        6219..6447
                     /label="RSV promoter"
     Promoter        6219..6628
                     /label="RSV/5' LTR hybrid promoter"
     Source          6218^6219
                     /label=Source_1
     LTR             6448..6628
                     /label="HIV-1 5' LTR"
     Misc._feature   6738
                     /label="splice donor"
     Misc._binding   6739..6783
                     /label="HIV-1 psi packaging signal"
     Protein_binding 7293..7526
                     /label="HIV-1 Rev response element"
     Misc._feature   7874
                     /label="splice acceptor"
     Misc._feature   7902
                     /label="splice acceptor"
     Promoter        8027..8609
                     /label="CMV promoter"
     TATA_box        8527..8530
                     /label="TATA box"
ORIGIN
        1 GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG ACTGGATCCG GTACCGAATT
       61 CACCATGGCT AGCTGGAGCC ACCCGCAGTT CGAAAAAGCC GAAAATTTGA AGCATATTAT
      121 CACCCTTGGC CAGGTCATCC ACAAACGGTG TGAAGAGATG AAATACTGCA AGAAACAGTG
      181 CCGGCGCCTG GGCCACCGCG TCCTCGGCCT GATCAAGCCT CTGGAGATGC TCCAGGACCA
      241 AGGAAAGAGG AGCGTGCCCT CTGAGAAGTT AACCACAGCC ATGAACCGCT TCAAGGCTGC
      301 CCTGGAGGAG GCTAATGGGG AGATAGAAAA GTTCAGCAAT AGATCCAATA TCTGCAGGTT
      361 TCTAACAGCA AGCCAGGACA AAATACTCTT CAAGGACGTG AACAGGAAGC TGAGTGATGT
      421 CTGGAAGGAG CTCTCGCTGT TACTTCAGGT TGAGCAACGC ATGCCTGTTT CACCCATAAG
      481 CCAAGGAGCG TCCTGGGCAC AGGAAGATCA GCAGGATGCA GACGAAGACA GGCGAGCTTT
      541 CCAGATGCTA AGAAGAGATA ATGAAAAAAT AGAAGCTTCA CTGAGACGAT TAGAAATCAA
      601 CATGAAAGAA ATCAAGGAAA CTTTGAGGCA GTATTTACCA CCAAAATGCA TGCAGGAGAT
      661 CCCGCAAGAG CAAATCAAGG AGATCAAGAA GGAGCAGCTT TCAGGATCCC CGTGGATTCT
      721 GCTAAGGGAA AATGAAGTCA GCACACTTTA TAAAGGAGAA TACCACAGAG CTCCAGTGGC
      781 CATAATGGTA TTCAAAAAAC TCCAGGCTGG CAGCATTGCA ATAGTGAGGC AGACTTTCAA
      841 TAAGGAGATC AAAACCATGA AGAAATTCGA ATCTCCCAAC ATCCTGCGTA TATTTGGGAT
      901 TTGCATTGAT GAAACAGTGA CTCCGCCTCA ATTCTCCATT GTCATGGAGT ACTGTGAACT
      961 CGGGACCCTG AGGGAGCTGT TGGATAGGGA AAAAGACCTC ACACTTGGCA AGCGCATGGT
     1021 CCTAGTCCTG GGGGCAGCCC GAGGCCTATA CCGGCTACAC CATTCAGAAG CACCTGAACT
     1081 CCACGGAAAA ATCAGAAGCT CAAACTTCCT GGTAACTCAA GGCTACCAAG TGAAGCTTGC
     1141 AGGATTTGAG TTGAGGAAAA CACAGACTTC CATGAGTTTG GGAACTACGA GAGAAAAGAC
     1201 AGACAGAGTC AAATCTACAG CATATCTCTC ACCTCAGGAA CTGGAAGATG TATTTTATCA
     1261 ATATGATGTA AAGTCTGAAA TATACAGCTT TGGAATCGTC CTCTGGGAAA TCGCCACTGG
     1321 AGATATCCCG TTTCAAGGCT GTAATTCTGA GAAGATCCGC AAGCTGGTGG CTGTGAAGCG
     1381 GCAGCAGGAG CCACTGGGTG AAGACTGCCC TTCAGAGCTG CGGGAGATCA TTGATGAGTG
     1441 CCGGGCCCAT GATCCCTCTG TGCGGCCCTC TGTGGATGAA ATCTTAAAGA AACTCTCCAC
     1501 CTTTTCTAAG CACTCGAGAT ATCTAGACCC AGCTTTCTTG TACAAAGTGG TTGATATCCA
     1561 GCACAGTGGC GGCCGCTCGA GTCTAGAGGG CCCGGACTAC AAGGATGACG ATGACAAAAC
     1621 GCGTACCGGT TAGTAATGAG TTTGGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT
     1681 GGAAAGTCCC CAGGCTCCCC AGGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC
     1741 AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA
     1801 TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC
     1861 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC
     1921 CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT
     1981 AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT
     2041 GACCATGGCC ACCGAGTACA AGCCCACGGT GCGCCTCGCC ACCCGCGACG ACGTCCCCCG
     2101 GGCCGTACGC ACCCTCGCCG CCGCGTTCGC CGACTACCCC GCCACGCGCC ACACCGTCGA
     2161 CCCGGACCGC CACATCGAGC GGGTCACCGA GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG
     2221 GCTCGACATC GGCAAGGTGT GGGTCGCGGA CGACGGCGCC GCGGTGGCGG TCTGGACCAC
     2281 GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT CGCCGAGATC GGCTCGCGCA TGGCCGAGTT
     2341 GAGCGGTTCC CGGCTGGCCG CGCAGCAACA GATGGAAGGC CTCCTGGCGC CGCACCGGCC
     2401 CAAGGAGCCC GCGTGGTTCC TGGCCACCGT CGGCGTCTCG CCCGACCACC AGGGCAAGGG
     2461 TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT GGAGGCGGCC GAGCGCGCTG GGGTGCCCGC
     2521 CTTCCTGGAG ACCTCCGCGC CCCGCAACCT CCCCTTCTAC GAGCGGCTCG GCTTCACCGT
     2581 CACCGCCGAC GTCGAGGTGC CCGAAGGACC GCGCACCTGG TGCATGACCC GCAAGCCCGG
     2641 TGCCTGAGCT AAGCACAATT CGAGCTCGGT ACCTTTAAGA CCAATGACTT ACAAGGCAGC
     2701 TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA
     2761 ACGAAGACAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC
     2821 CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG
     2881 AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG
     2941 ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT TCATGTCATC TTATTATTCA
     3001 GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG AGGAACTTGT TTATTGCAGC
     3061 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC
     3121 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGCTCTA
     3181 GCTATCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT
     3241 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT
     3301 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGGACGTA CCCAATTCGC
     3361 CCTATAGTGA GTCGTATTAC GCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG
     3421 AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC
     3481 GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG
     3541 AATGGGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG
     3601 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC
     3661 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC
     3721 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA
     3781 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA
     3841 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG
     3901 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA
     3961 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTAGGTGGCA CTTTTCGGGG
     4021 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
     4081 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
     4141 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
     4201 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
     4261 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
     4321 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
     4381 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
     4441 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
     4501 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
     4561 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
     4621 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
     4681 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
     4741 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
     4801 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
     4861 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
     4921 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
     4981 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
     5041 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
     5101 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
     5161 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
     5221 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
     5281 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
     5341 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
     5401 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
     5461 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
     5521 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
     5581 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
     5641 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
     5701 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
     5761 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC
     5821 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC
     5881 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC
     5941 AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG
     6001 GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA
     6061 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG
     6121 CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA
     6181 CCCTCACTAA AGGGAACAAA AGCTGGAGCT GCAAGCTTAA TGTAGTCTTA TGCAATACTC
     6241 TTGTAGTCTT GCAACATGGT AACGATGAGT TAGCAACATG CCTTACAAGG AGAGAAAAAG
     6301 CACCGTGCAT GCCGATTGGT GGAAGTAAGG TGGTACGATC GTGCCTTATT AGGAAGGCAA
     6361 CAGACGGGTC TGACATGGAT TGGACGAACC ACTGAATTGC CGCATTGCAG AGATATTGTA
     6421 TTTAAGTGCC TAGCTCGATA CATAAACGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG
     6481 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG
     6541 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC
     6601 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG
     6661 GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG
     6721 GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA
     6781 GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA
     6841 TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA
     6901 GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC
     6961 AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT
     7021 ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG
     7081 AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG
     7141 CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT
     7201 AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA
     7261 GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA
     7321 GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA
     7381 TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT
     7441 CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA
     7501 AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC
     7561 ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT
     7621 CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC
     7681 TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT
     7741 AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA
     7801 TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA
     7861 GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG
     7921 AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA
     7981 TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGATAAG CTTGGGAGTT CCGCGTTACA
     8041 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA
     8101 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG
     8161 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG
     8221 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC
     8281 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG
     8341 ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA
     8401 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT
     8461 CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG
     8521 GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA
     8581 CGCTGTTTTG ACCTCCATAG AAGACACCGA CTCTAGAGGA TCCACTAGTC CAGTGTGGTG
     8641 GAATTCTGCA GATATCAACA AGTTT
//
