LOCUS       pLenti6(puro)-FLAG-dest-strep-mRIP1-stop        9220 bp    DNA     circular SYN 18-MAR-2015
DEFINITION  LMBP 8806.
ACCESSION   pIB0097 - pLenti6/FLAG-DEST-puro
SOURCE      
  ORGANISM  
COMMENT     Accession number: 8806
            Last modified: 18-MAR-2015
            
COMMENT     VNTDATE|728671520|
COMMENT     VNTNAME|pLenti6(puro)-FLAG-dest-strep-mRIP1-stop|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTREPLTYPE|Virus
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 0 9220 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
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COMMENT        (CWordArray) (CStringList "AgeI" "ApaI" "NdeI" "NheI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 955 951 1 9220 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "AgeI" "accggt" 2 0 1 90 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "ApaI" "gggccc" 6 0 2 57 0 7379 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NdeI" "catatg" 3 0 1 6672 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 7198 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 5 114 0 2785 0 5303 0 
COMMENT            7160 0 8184 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "FLAG tag" 21 0 0 58 82 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "58..82") 
COMMENT        #6=(CFSignal (CObList) "SV40 early promoter" 29 0 0 118 400 0 
COMMENT            "140..449") 
COMMENT        #7=(CFSignal (CObList) "puromycin resistance" 4 0 0 509 1111 0 
COMMENT            "509..1111") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 0 2597 3457 0 
COMMENT            "2597..3457") 
COMMENT        #9=(CFSignal (CObList) "HIV-1 psi packaging signal" 21 0 0 5203 5247 0 
COMMENT            "5203..5247") 
COMMENT        #10=(CFSignal (CObList) "HIV-1 Rev response element" 21 0 0 5757 5990 0 
COMMENT             "5757..5990") 
COMMENT        #11=(CFSignal (CObList) "strep" 4 0 0 7194 7229 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "mRIPK1-start-stop" 4 0 0 7230 9194 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "7230..9194") 
COMMENT        #13=(CFSignal (CObList) "T3 promoter" 30 0 0 4639 4655 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "pMB1 ori" 33 0 0 3998 4400 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "lac promoter" 30 0 0 4492 4575 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "RSV/HIV-1 5' LTR" 29 0 0 4764 5092 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 6489 7005 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "SV40 ori" 33 0 0 357 432 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "HIV-1 cPPT" 21 0 0 1186 1201 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #20=(CFSignal (CObList) "RSV/HIV-1 dU3/3' LTR" 43 0 0 1202 1436 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1508 1582 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #22=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1508 1582 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #23=(CFSignal (CObList) "SV40 ori" 33 0 0 1718 1793 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #24=(CFSignal (CObList) "T7 promoter" 30 0 1 1827 1843 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #25=(CFSignal (CObList) "f1 ori" 33 0 0 2011 2466 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #26=(CFSignal (CObList) "attB1" 0 0 0 7122 7146 0 (CStringList) 
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COMMENT                          "DNA Plasmid Phage Virus 'pLenti6(puro)-FLAG-dest-strep-mRIP1-stop'" 
COMMENT                          1) 
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COMMENT                          "Collection N.\260: 8806." 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Created: 03/17/15 04:45" 1) 
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COMMENT                          "Last Modified: 03/18/15 09:54" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9220 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Division: SYN" 1) 
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COMMENT                          "Modification Date in the Original DB: 17-MAR-2015" 1) 
COMMENT                     #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/pIB0097\">pIB0097</a>; <a href=\"ncbilink:dnarna/-\">-</a>; <a href=\"ncbilink:dnarna/pLenti6/FLAG-DEST-puro\">pLenti6/FLAG-DEST-puro</a>" 
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COMMENT                   "Restriction/Methylation Map") 
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COMMENT                                   "{\\i Xmn}I (7160)" "@N (@S)" 5 -17.5046 
COMMENT                                   2.51912 0 -10 4.66076 1.04123 #336#) 
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COMMENT               #340=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -675 100) 
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COMMENT                            "Collection N\260.: 8806" "Collection N\260.: 8806" 
COMMENT                            65535 0.0495828 -2.87578 0 -10 12.0816 1.60317 Nil) 
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COMMENT                            1.74233 0.888889 0 "strep-mRIP1-stop" 
COMMENT                            "strep-mRIP1-stop" 65535 -0.0247913 0.39666 0 -10 
COMMENT                            10.4289 1.53706 Nil) 
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COMMENT       (CStringList) 34078830 14746016 
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COMMENT        #345=(CSampFrag "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 2671 114 
COMMENT              "XmnI" 2785 "XmnI" (LOGPEN 0 0 0) 1.37913e-316 2.18422e-300) 
COMMENT        #346=(CSampFrag "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 2518 2785 
COMMENT              "XmnI" 5303 "XmnI" (LOGPEN 0 0 0) 9.1033e-283 4.69778e-294) 
COMMENT        #347=(CSampFrag "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 1857 5303 
COMMENT              "XmnI" 7160 "XmnI" (LOGPEN 0 0 0) 9.10449e-283 9.10429e-283) 
COMMENT        #348=(CSampFrag "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 1150 8184 
COMMENT              "XmnI" 114 "XmnI" (LOGPEN 0 0 0) 9.10685e-283 4.69761e-294) 
COMMENT        #349=(CSampFrag "pLenti6(puro)-FLAG-dest-strep-mRIP1-stop" 1024 7160 
COMMENT              "XmnI" 8184 "XmnI" (LOGPEN 0 0 0) 9.10567e-283 4.69748e-294))))
FEATURES             Location/Qualifiers
     Misc._recombina complement(join(9211..9220,1..>7))
                     /vntifkey="21"
                     /label=attL2
                     /note="Unknown feature type:Misc._recombina"
     Misc._recombina complement(join(9214..9220,1..14))
                     /vntifkey="21"
                     /label=attB2
                     /note="Unknown feature type:Misc._recombina"
     Misc._feature   58..82
                     /vntifkey="21"
                     /label=FLAG\tag
                     /note="Unknown feature type:Misc._feature"
     promoter        118..400
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             509..1111
                     /vntifkey="4"
                     /label=puromycin\resistance
     CDS             2597..3457
                     /vntifkey="4"
                     /label=ampicillin\resistance
     Source          4682^4683
                     /vntifkey="21"
                     /label=Source_1
                     /note="Unknown feature type:Source  Oriinal location: 4175..4174"
     Misc._binding   5203..5247
                     /vntifkey="21"
                     /label=HIV-1\psi\packaging\signal
                     /note="Unknown feature type:Misc._binding  Oriinal location: 4695..4739"
     Protein_binding 5757..5990
                     /vntifkey="21"
                     /label=HIV-1\Rev\response\element
                     /note="Unknown feature type:Protein_binding  Oriinal location: 5249..5482"
     Source          join(<9198..>9220,<7130..>7185)
                     /vntifkey="21"
                     /label=Source_1
                     /note="Unknown feature type:Source"
     Vector          join(9198..>9220,<7130..7185)
                     /origin="pENTR3C (2.723bp circular)Target vector: fromXhoI
                     cut at 938^939 to EcoRI cut at 496^497,2.281bpOrientation:
                     originalPlus strand 5':   nochangePlus strand 3':   no
                     changeMinus strand 5':  nochangeMinus strand 3':  no
                     change"
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Unknown feature type:Vector"
     CDS             7194..7229
                     /vntifkey="4"
                     /label=strep
     CDS             7230..9194
                     /vntifkey="4"
                     /label=mRIPK1-start-stop
     promoter        4639..4655
                     /vntifkey="30"
                     /label=T3\promoter
     rep_origin      3998..4400
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        4492..4575
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        4764..5092
                     /vntifkey="29"
                     /label=RSV/HIV-1\5'\LTR
     promoter        6489..7005
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      357..432
                     /vntifkey="33"
                     /label=SV40\ori
     misc_feature    1186..1201
                     /vntifkey="21"
                     /label=HIV-1\cPPT
     terminator      1202..1436
                     /vntifkey="43"
                     /label=RSV/HIV-1\dU3/3'\LTR
     terminator      1508..1582
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1508..1582)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      1718..1793
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1827..1843)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      2011..2466
                     /vntifkey="33"
                     /label=f1\ori
     attenuator      7122..7146
                     /vntifkey="0"
                     /label=attB1
     attenuator      complement(9211..15)
                     /vntifkey="0"
                     /label=attB2
BASE COUNT     2493 a      2223 c      2365 g      2139 t 
ORIGIN
        1 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgga 
       61 ctacaaggat gacgatgaca aaacgcgtac cggttagtaa tgagtttgga attaattctg 
      121 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat 
      181 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 
      241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 
      301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 
      361 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 
      421 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 
      481 cattttcgga tctgatcagc acgtgaccat ggccaccgag tacaagccca cggtgcgcct 
      541 cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt tcgccgacta 
      601 ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca ccgagctgca 
      661 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg 
      721 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga 
      781 gatcggctcg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga 
      841 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt 
      901 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc 
      961 ggccgagcgc gctggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt 
     1021 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac 
     1081 ctggtgcatg acccgcaagc ccggtgcctg agctaagcac aattcgagct cggtaccttt 
     1141 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 
     1201 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc 
     1261 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
     1321 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
     1381 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 
     1441 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 
     1501 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     1561 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     1621 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta 
     1681 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 
     1741 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 
     1801 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg 
     1861 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 
     1921 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 
     1981 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg 
     2041 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     2101 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     2161 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 
     2221 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     2281 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     2341 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 
     2401 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta 
     2461 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 
     2521 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 
     2581 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 
     2641 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 
     2701 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 
     2761 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 
     2821 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 
     2881 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 
     2941 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 
     3001 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 
     3061 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 
     3121 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 
     3181 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 
     3241 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 
     3301 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 
     3361 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 
     3421 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 
     3481 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 
     3541 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 
     3601 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 
     3661 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 
     3721 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 
     3781 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 
     3841 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 
     3901 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 
     3961 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 
     4021 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 
     4081 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 
     4141 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 
     4201 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 
     4261 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 
     4321 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 
     4381 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 
     4441 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 
     4501 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 
     4561 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca 
     4621 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc 
     4681 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa 
     4741 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac 
     4801 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa 
     4861 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc 
     4921 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 
     4981 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 
     5041 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc 
     5101 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc 
     5161 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt 
     5221 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga 
     5281 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat 
     5341 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt 
     5401 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag 
     5461 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa 
     5521 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa 
     5581 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg 
     5641 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 
     5701 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 
     5761 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 
     5821 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 
     5881 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 
     5941 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 
     6001 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 
     6061 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 
     6121 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 
     6181 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 
     6241 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 
     6301 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 
     6361 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 
     6421 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 
     6481 taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 
     6541 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 
     6601 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 
     6661 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 
     6721 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 
     6781 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag 
     6841 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt 
     6901 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 
     6961 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt 
     7021 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgactctag 
     7081 aggatccact agtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag 
     7141 caggctcttt aaaggaacca attcagtcga ctggatccgg taccgaattc accatggcta 
     7201 gctggagcca cccgcagttc gaaaaagccc aaccagacat gtccttggac aatattaaga 
     7261 tggcatccag tgacctgctg gagaagacag acctagacag cggaggcttc gggaaggtgt 
     7321 ccttgtgtta ccacagaagc catggatttg tcatcctgaa aaaagtatac acagggccca 
     7381 accgcgctga gtacaatgag gttctcttgg aagaggggaa gatgatgcac agactgagac 
     7441 acagtcgagt ggtgaagcta ctgggcatca tcatagaaga agggaactat tcgctggtga 
     7501 tggagtacat ggagaagggc aacctgatgc acgtgctaaa gacccagata gatgtcccac 
     7561 tttcattgaa aggaaggata atcgtggagg ccatagaagg catgtgctac ttacatgaca 
     7621 aaggtgtgat acacaaggac ctgaagcctg agaatatcct cgttgatcgt gactttcaca 
     7681 ttaagatagc cgatcttggt gtggcttcct ttaagacatg gagcaaactg actaaggaga 
     7741 aagacaacaa gcagaaagaa gtgagcagca ccactaagaa gaacaatggt ggtacccttt 
     7801 actacatggc acccgaacac ctgaatgaca tcaatgcaaa gcccacggag aagtcggacg 
     7861 tgtacagctt tggcattgtc ctttgggcaa tatttgcaaa aaaggagccc tatgagaatg 
     7921 tcatctgtac tgagcagttc gtgatctgca taaaatctgg gaacaggcca aatgtagagg 
     7981 aaatccttga gtactgtcca agggagatca tcagcctcat ggagcggtgc tggcaggcga 
     8041 tcccagaaga caggccaaca tttcttggca ttgaagaaga atttaggcct ttttacttaa 
     8101 gtcattttga agaatatgta gaagaggatg tggcaagttt aaagaaagag tatccagatc 
     8161 aaagcccagt gctgcagaga atgttttcac tgcagcatga ctgtgtaccc ttacctccga 
     8221 gcaggtcaaa ttcagaacaa cctggatcgc tgcacagttc ccaggggctc cagatgggtc 
     8281 ctgtggagga gtcctggttt tcttcctccc cagagtaccc acaggacgag aatgatcgca 
     8341 gtgtgcaggc taagctgcaa gaggaagcca gctatcatgc ttttggaata tttgcagaga 
     8401 aacagacaaa accgcagcca aggcagaatg aggcttacaa cagagaggag gaaaggaaac 
     8461 gaagggtctc tcatgacccc tttgcacagc agagagctcg tgagaatatt aagagtgcag 
     8521 gagcaagagg tcattctgat cccagcacaa cgagtcgtgg aattgcagtg caacagctgt 
     8581 catggccagc cacccaaaca gtttggaaca atggattgta taatcagcat ggatttggaa 
     8641 ctacaggtac aggagtttgg tatccgccaa atctaagcca aatgtatagt acttataaaa 
     8701 ctccagtgcc agagaccaac ataccgggaa gcacacccac catgccatac ttctctgggc 
     8761 cagtagcaga tgacctcata aaatatacta tattcaatag ttctggtatt cagattggaa 
     8821 accacaatta tatggatgtt ggactgaatt cacaaccacc aaacaatact tgcaaagaag 
     8881 agtcgacttc cagacaccaa gccatctttg ataacaccac tagtctgact gatgaacacc 
     8941 tgaaccctat cagggaaaac ctgggaaggc agtggaaaaa ctgtgcccgc aagctgggct 
     9001 tcactgagtc tcagatcgat gaaatcgacc atgactatga aagagatgga ctgaaagaga 
     9061 aagtttacca aatgcttcag aagtggctga tgcgggaagg caccaaaggg gccacagtgg 
     9121 gaaagttggc ccaggcactt caccaatgtt gcaggataga cctgctgaac cacttgattc 
     9181 gtgccagcca gagccactcg agatatctag acccagcttt 
//
