LOCUS       pLenti6(puro)-FLAG-dest-strep-hMLKL(1-210)      7882 bp    DNA     circular SYN 26-FEB-2013
DEFINITION  LMBP 8808.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES             Location/Qualifiers
     Misc._recombina <1..17
                     /label=attL1
     Misc._recombina join(7876..7882,1..14)
                     /label=attB1
     Source          join(<731..>753,<1..>56)
                     /label=Source_1
     Vector          join(731..>753,<1..56)
                     /origin="pENTR3C (2.723bp circular)Target vector: fromXhoI
                     cut at 938^939 to EcoRI cut at 496^497,2.281bpOrientation:
                     originalPlus strand 5':   nochangePlus strand 3':   no
                     changeMinus strand 5':  nochangeMinus strand 3':  no
                     change"
     PCR_fragment    join(<57..125,126..708,complement(709..>734))
                     /note="Forward primer name: YD255-FWhMLKL+strep-start(EZ)"
                     /note="Forward
                     primer:atccggtaccgaattcaccatggctagctggagccacccgcagttcgaaaaa
                     gccGAAAATTTGAAGCATATTATCACCC"
                     /note="Forward primer misprimes: 55"
                     /note="Reverse primer name: YD237-RV hMLKL Nterm(-stop)"
                     /note="Reverse
                     primer:gtctagataTctcgagTgCCTTAGCAGAATCCACGGG"
                     /note="Reverse primer misprimes: 16"
                     /note="Fragment length: 700"
                     /label="Fragment (hMLKL (YD255-FW hMLKL+strep-start(EZ) -
                     YD237-RV hMLKL Nterm(-stop)))"
                     /label="Fragment (hMLKL (YD255-FW hMLKL+strep-start(EZ) - YD237-RV hMLKL Nterm(-stop)))"
     CDS             65..100
                     /label=strep
     CDS             101..727
                     /label="hMLKL 1-210-start"
                     /label="hMLKL 1-210-start"
     Misc._recombina complement(744..>760)
                     /label=attL2
     Misc._recombina complement(747..767)
                     /label=attB2
     Misc._feature   811..835
                     /label="FLAG tag"
     CDS             848..856
                     /label="3 stops"
     Promoter        893..1202
                     /label="SV40 early promoter"
     CDS             1262..1864
                     /label=Puro(R)
     LTR             1955..2008
                     /label=deltaU3
     LTR             1956..2189
                     /label="deltaU3/3' LTR"
     LTR             2009..2189
                     /label="HIV-1 3' LTR"
     PolyA_signal    2261..2391
                     /label="SV40 pA"
     Replication_ori 2764..3219
                     /label="f1 origin"
     Promoter        3251..3349
                     /label="bla promoter"
     CDS             3350..4210
                     /label=Amp(R)
     Replication_ori 4355..5028
                     /label="pUC origin"
     Promoter        5436..5664
                     /label="RSV promoter"
     Promoter        5436..5845
                     /label="RSV/5' LTR hybrid promoter"
     Source          5435^5436
                     /label=Source_1
     LTR             5665..5845
                     /label="HIV-1 5' LTR"
     Misc._feature   5955
                     /label="splice donor"
     Misc._binding   5956..6000
                     /label="HIV-1 psi packaging signal"
     Protein_binding 6510..6743
                     /label="HIV-1 Rev response element"
     Misc._feature   7091
                     /label="splice acceptor"
     Misc._feature   7119
                     /label="splice acceptor"
     Promoter        7244..7826
                     /label="CMV promoter"
     TATA_box        7744..7747
                     /label="TATA box"
ORIGIN
        1 GTACAAAAAA GCAGGCTCTT TAAAGGAACC AATTCAGTCG ACTGGATCCG GTACCGAATT
       61 CACCATGGCT AGCTGGAGCC ACCCGCAGTT CGAAAAAGCC GAAAATTTGA AGCATATTAT
      121 CACCCTTGGC CAGGTCATCC ACAAACGGTG TGAAGAGATG AAATACTGCA AGAAACAGTG
      181 CCGGCGCCTG GGCCACCGCG TCCTCGGCCT GATCAAGCCT CTGGAGATGC TCCAGGACCA
      241 AGGAAAGAGG AGCGTGCCCT CTGAGAAGTT AACCACAGCC ATGAACCGCT TCAAGGCTGC
      301 CCTGGAGGAG GCTAATGGGG AGATAGAAAA GTTCAGCAAT AGATCCAATA TCTGCAGGTT
      361 TCTAACAGCA AGCCAGGACA AAATACTCTT CAAGGACGTG AACAGGAAGC TGAGTGATGT
      421 CTGGAAGGAG CTCTCGCTGT TACTTCAGGT TGAGCAACGC ATGCCTGTTT CACCCATAAG
      481 CCAAGGAGCG TCCTGGGCAC AGGAAGATCA GCAGGATGCA GACGAAGACA GGCGAGCTTT
      541 CCAGATGCTA AGAAGAGATA ATGAAAAAAT AGAAGCTTCA CTGAGACGAT TAGAAATCAA
      601 CATGAAAGAA ATCAAGGAAA CTTTGAGGCA GTATTTACCA CCAAAATGCA TGCAGGAGAT
      661 CCCGCAAGAG CAAATCAAGG AGATCAAGAA GGAGCAGCTT TCAGGATCCC CGTGGATTCT
      721 GCTAAGGCAC TCGAGATATC TAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCCAGCA
      781 CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC GGACTACAAG GATGACGATG ACAAAACGCG
      841 TACCGGTTAG TAATGAGTTT GGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA
      901 AAGTCCCCAG GCTCCCCAGG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC
      961 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT
     1021 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC
     1081 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA
     1141 GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG
     1201 CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGAC
     1261 CATGGCCACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC
     1321 CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC
     1381 GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT
     1441 CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC
     1501 GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC TCGCGCATGG CCGAGTTGAG
     1561 CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA
     1621 GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT
     1681 GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCTGGGG TGCCCGCCTT
     1741 CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC
     1801 CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC
     1861 CTGAGCTAAG CACAATTCGA GCTCGGTACC TTTAAGACCA ATGACTTACA AGGCAGCTGT
     1921 AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG
     1981 AAGACAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG
     2041 GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT
     2101 GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC
     2161 CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA TGTCATCTTA TTATTCAGTA
     2221 TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG AACTTGTTTA TTGCAGCTTA
     2281 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT
     2341 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGCTCTAGCT
     2401 ATCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG
     2461 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC GGCCTCTGAG
     2521 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GGACGTACCC AATTCGCCCT
     2581 ATAGTGAGTC GTATTACGCG CGCTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA
     2641 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA
     2701 ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT
     2761 GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA
     2821 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG
     2881 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT
     2941 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG
     3001 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA
     3061 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT
     3121 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT
     3181 TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA
     3241 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
     3301 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA
     3361 ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA
     3421 CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA
     3481 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT
     3541 TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC
     3601 CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC
     3661 ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC
     3721 CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA
     3781 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA
     3841 ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT
     3901 GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA
     3961 ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC
     4021 GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT
     4081 TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG
     4141 TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA
     4201 GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA
     4261 TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC
     4321 TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC
     4381 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC
     4441 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT
     4501 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
     4561 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC
     4621 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA
     4681 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC
     4741 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG
     4801 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
     4861 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT
     4921 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA
     4981 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC
     5041 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG
     5101 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT
     5161 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
     5221 TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA
     5281 GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG
     5341 ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG CAATTAACCC
     5401 TCACTAAAGG GAACAAAAGC TGGAGCTGCA AGCTTAATGT AGTCTTATGC AATACTCTTG
     5461 TAGTCTTGCA ACATGGTAAC GATGAGTTAG CAACATGCCT TACAAGGAGA GAAAAAGCAC
     5521 CGTGCATGCC GATTGGTGGA AGTAAGGTGG TACGATCGTG CCTTATTAGG AAGGCAACAG
     5581 ACGGGTCTGA CATGGATTGG ACGAACCACT GAATTGCCGC ATTGCAGAGA TATTGTATTT
     5641 AAGTGCCTAG CTCGATACAT AAACGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG
     5701 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT
     5761 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT
     5821 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA
     5881 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG
     5941 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG
     6001 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC
     6061 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG
     6121 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA
     6181 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA
     6241 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG
     6301 CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG CAAGCGGCCG
     6361 CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA
     6421 TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA GAGAAGAGTG
     6481 GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA
     6541 GCAGGAAGCA CTATGGGCGC AGCGTCAATG ACGCTGACGG TACAGGCCAG ACAATTATTG
     6601 TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA ACAGCATCTG
     6661 TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA
     6721 TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC
     6781 ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC
     6841 ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA
     6901 ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA
     6961 TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC
     7021 ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG
     7081 AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC AACCCCGAGG
     7141 GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG AGACAGATCC
     7201 ATTCGATTAG TGAACGGATC TCGACGGTAT CGATAAGCTT GGGAGTTCCG CGTTACATAA
     7261 CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA
     7321 ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG
     7381 TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC
     7441 CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA
     7501 TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG
     7561 CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT
     7621 CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA
     7681 AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG
     7741 GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC
     7801 TGTTTTGACC TCCATAGAAG ACACCGACTC TAGAGGATCC ACTAGTCCAG TGTGGTGGAA
     7861 TTCTGCAGAT ATCAACAAGT TT
//
